CRA ISF Peach Fruit Mesocarp Project Description

Peach (Prunus persica) represents a model species for prunus and a reference species for Rosaceae, To develop an extensive Prunus EST database for identification and cloning of genes important to fruit and tree development, we generated 9984 high-quality expressed sequence tags (ESTs) from a peach cDNA library of developing fruit mesocarp. After assembly and annotation, a putative peach unigene set consisting of 3842 ESTs was defined.

Further annotation includes Gene Ontology (GO) classification and computational detection of simple sequence repeats (SSR) and open reading frame (ORF). Using core markers from the general Prunus genetic map, BAC clones were anchored on the genetic map providing a framework for construction of a physical and a transcript map. To develop a transcript map, 1236 ESTs from the putative peach unigene set and additional 68 peach cDNA clones were hybridized against the peach BAC library. Hybridizing ESTs to genetically anchored BACs immediately localized 11.2% of the ESTs on the genetic map.

October 29, 2002 : Abbott Peach ESTs Analyzed for SSRs

August 20, 2002  : Abbott Peach ESTs Submitted to GenBank

• Description
• Libraries Information
• Protocol/Downloads
• Unigene Details
• Gene Homology
• Microsatellite Analysis
• Download Data
• Contact
• Publication