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Description:
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Genetic linkage maps for two apricot cultivars
have been constructed using AFLP, RAPD, RFLP and
SSR markers in 81 F1 individuals from the cross 'Goldrich'
W 'Valenciano'. This family segregated for resistance
to 'plum pox virus' (PPV), the most-important virus
affecting Prunus species. Of the 160 RAPD arbitrary
primers screened a total of 44 were selected. Sixty one
polymorphic RAPD markers were scored on the mapping
population: 30 heterozygous in 'Goldrich', 19 heterozygous
in 'Valenciano', segregating 1:1, and 12 markers
heterozygous in both parents, segregating 3:1. A total of
33 and 19 RAPD markers were mapped on the 'Goldrich'
and 'Valenciano' maps respectively. Forteen primer combinations
were used for AFLPs and all of them detected
polymorphism. Ninety five markers segregating 1:1 were
identified, of which 62 were heterozygous in the female
parent 'Goldrich' and 33 in the male parent 'Valenciano'.
Forty five markers were present in both parents and segregated
3:1. A total of 82 and 48 AFLP markers were
mapped on the 'Goldrich' and 'Valenciano' maps. Twelve
RFLPs probes were screened in the population, resulting
in five loci segregating in the family, one locus heterozygous
for 'Valenciano' and four heterozygous for both,
segregating 1:2:1. Of the 45 SSRs screened 17 segregated
in the mapping family, resulting in seven loci heterozygous
for the maternal parent and ten heterozygous for
both, segregating 1:2:1 or 1:1:1:1. A total of 16 and 13
co-dominant markers were mapped in the female and
male parent maps respectively. A total of 132 markers
were placed into eight linkage groups on the 'Goldrich'. |
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