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| Map Set Name: |
Fragaria-815x903-BC1 |
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| Abbreviated Name: |
Fragaria-815x903-BC1 |
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| Accession ID: |
36 |
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| Species: |
Fragaria (Strawberry) |
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| Map Type: |
Genetic |
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| Map Units: |
cM |
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| Published On: |
01 May, 2006 |
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Description:
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We have produced a reduced linkage map of a diploid Fragaria backcross progeny (F. vesca W [F. vesca W F. viridis]) denoted 815 x 903BC from 31 microsatellite (SSR) and two gene-specific markers selected from the diploid Fragaria reference map FV W FN. The 33 sequence tagged-site (STS) markers selected, segregated in seven linkage groups, and marker order was conserved between FV W FN and 815 x 903BC. Significant segregation distortion, observed on three of the seven linkage groups, was accompanied by a reduction in observed recombination frequency and, therefore, genetic distance between markers mapped in these linkage groups. However, this distortion did not affect marker order compared to FV W FN and may indicate non-random chromosomal divergence during speciation in Fragaria. Our findings indicate that no major genome reorganisation has occurred during speciation in the genus, and suggest that STS linkage maps of other Fragaria populations, including those of the cultivated strawberry F. W ananassa, could be constructed using the diploid Fragaria reference map FV W FN as a standard reference point. |
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Updates:
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Sargent et al. The development and mapping of functional markers in Fragaria and their transferability and potential for mapping in other genera. Accepted for TAG
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Abstract of the updates:
We have developed 46 primer pairs from exon sequences flanking polymorphic introns of 23 Fragaria gene sequences and one Malus sequence deposited in the EMBL database. Sequencing of a set of the PCR products amplified with the novel primer pairs in diploid Fragaria showed the products to be homologous to the sequences from which the primers were originally designed. By scoring the segregation of the 24 genes in two diploid Fragaria progenies FVWFN (F. vesca W F. nubicola F2) and 815W903BC (F. vesca W F. viridis BC1) 29 genetic loci at discrete positions on the seven linkage groups previously characterised could be mapped, bringing to 35 the total number of known function genes mapped in Fragaria. Twenty primer pairs, representing 14 genes, amplified a product of the expected size in both Malus and Prunus. To demonstrate the applicability of these gene-specific loci to comparative mapping in Rosaceae, five markers that displayed clear polymorphism between the parents of a Malus and a Prunus mapping population were selected. The markers were then scored and mapped in at least one of the two additional progenies. |
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