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| Map Set Name: |
Almond-FT-F1 |
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| Abbreviated Name: |
Almond-FT-F1 |
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| Accession ID: |
101 |
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| Species: |
Prunus amygdalus (Almond) |
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| Map Type: |
Genetic |
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| Map Units: |
cM |
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| Published On: |
17 March, 2010 |
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FxT linkage map:
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Fifty-four RAPD (random amplified polymorphic DNA) markers and 6 SSRs (simple sequence repeats) were included in a molecular marker map with 120 RFLPs (restriction fragment length polymorphisms) and 7 isozyme genes previously constructed using the offspring of a cross between the almond (Prunus amygdalus) cultivars 'Ferragnes' and 'Tuono'. Only highly reproducible RAPDs segregating 1:1 were used. To identify these markers, a total of 325 primers were screened, from which 41 produced RAPDs useful for mapping. Polymorphism was detected in six of the eight Prunus SSRs (simple sequence repeats) studied, thus enabling these to be mapped. All markers were placed on the 8 linkage groups previously identified. The number of new markers included in the map of 'Ferragnes' was 33 for a total of 126, and 30 in the map of 'Tuono' for a total of 99. The sizes of the maps of 'Ferragnes' (415 cM) and 'Tuono' (416 cM) were similar, representing a 5% increase over the maps constructed solely with isozymes and RFLPs. The estimated total size of the almond map was of 457 cM. Some markers were placed in zones with low density of markers and others in the extreme of linkage groups. The use of RAPD markers to complete genetic maps constructed with transferable markers is discussed. |
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Location of the self-incompatibility gene:
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A progeny obtained from the almond cross 'Ferragnhs' W 'Tuono' (Prunus amygdalus Batsch) was used to study the self-incompatibility trait in three different ways: fruit set, pollen tube growth and stylar ribonuclease activity. As expected from the genotypes of the parents, all progeny appeared phenotypically as self-compatible. However, the progeny could be scored for the segregation of stylar ribonuclease isozymes and thus allowed the incompatibility locus to be placed on the almond linkage map. |
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