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| Map Set Name: |
Pear-Bartlett |
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| Abbreviated Name: |
Pear-Bartlett |
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| Accession ID: |
44 |
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| Species: |
Pyrus (Pear) |
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| Map Type: |
Genetic |
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| Map Units: |
cM |
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| Published On: |
01 December, 2004 |
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| Map Set Name: |
Pear-Housui |
[ Show Only This Set ] |
| Abbreviated Name: |
Pear-Housui |
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| Accession ID: |
45 |
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| Species: |
Pyrus (Pear) |
[ View Species Info ] |
| Map Type: |
Genetic |
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| Map Units: |
cM |
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| Published On: |
01 December, 2004 |
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Description (abstract):
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Genetic linkage maps of the Japanese pear (Pyrus pyrifolia Nakai) cultivar 'Housui' and the European pear (Pyrus communis L.) cultivar 'Bartlett' were constructed based on Amplified Fragment Length Polymorphism markers (AFLPs), Simple Sequence Repeat markers (SSRs) (from pear, apple and Prunus), isozymes, and phenotypic traits by using their F1 progenies. The map of the female parent 'Bartlett' consisted of 256 loci including 178 AFLPs, 76 SSRs (32 pear, 39 apple, 5 Prunus SSRs), 1 isozyme and a self-incompatibility locus on 19 linkage groups over a total length of 1020 cM. The map of 'Housui' contained 180 loci including 110 AFLPs, 64 SSRs (29 pear, 29 apple, 6 Prunus SSRs), 2 phenotypic traits and 4 other markers on 20 linkage groups encompassing a genetic distance of 995 cM. These 2 pear maps were aligned using 37 co-dominant markers that showed segregating alleles in both parents. Out of 80 tested SSR markers developed from apple, more than four-fifth could produce discrete amplified fragments in pear. Thirty-eight apple SSR markers showed 39 segregating loci in the linkage map of 'Bartlett', while 27 markers produced 29 loci in 'Housui'. All pear linkage groups could be successfully aligned to the apple consensus map by at least 1 apple SSRs, suggesting that positions and linkages of SSR loci were well conserved between pear and apple. The self-incompatibility locus (S locus) was mapped to linkage group 17 in Japanese and European pears as well as apple. Our results are the first major effort in comparative mapping of pear and apple. |
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Description:
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Population name: BH
Cross combination: Bartlett (Pyrus communis) x Housui (Pyrus pyrifolia)
Population type: F1
Number of individuals: 63
Number of loci: 177
Number of linkage groups: 20
Mapping software: JoinMap ver. 2
LOD value for grouping: 6.0 |
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| Map Set Name: |
Pear-Kin1a-F1 |
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| Abbreviated Name: |
Kinchaku1a |
[ Download Map Set Data ] |
| Accession ID: |
46 |
[ View Map Set In Matrix ] |
| Species: |
Pyrus (Pear) |
[ View Species Info ] |
| Map Type: |
Genetic |
[ View Map Type Info ] |
| Map Units: |
cM |
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| Published On: |
01 August, 2006 |
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Description (abstract:
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Pear scab (caused by Venturia nashicola) is one of the most harmful diseases of pears, especially Japanese and Chinese pear species. The molecular identification and early selection of resistant plants could greatly improve pear breeding. We have identified the position of the scab resistance gene, designated Vnk in an indigenous Japanese pear cultivar Kinchaku, within the pear genome by using simple sequence repeat (SSR) markers derived from pear and apple. The position of Vnk was identified in the central region of linkage group 1 of Kinchaku. Several amplified fragment length polymorphism (AFLP) markers linked to Vnk were obtained by bulked segregant analysis. Among them, the AFLP marker closest to Vnk was converted into a sequence tagged site (STS) marker. Four random amplified polymorphic DNA (RAPD) markers previously found to be loosely associated with Vnk (Iketani et al. 2001) were successfully converted into STS markers. Six markers (one SSR Hi02c07 and five STSs converted from AFLP and RAPD) showed tight linkages to Vnk, being mapped with distances ranging from 2.4 to 12.4 cM. The SSR CH-Vf2, which was isolated from a BAC clone of the contig containing the apple scab gene Vf, was mapped at the bottom of linkage group 1 in Kinchaku, suggesting that the Vnk and Vf loci are located in different genomic regions of the same homologous linkage group. |
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Description:
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Population name: Shuurei x 314-32
Cross combination: Shuurei x 314-32 (Kinchaku x Housui)
Population type: F1
Number of individuals: 112
Number of loci: 14
Number of linkage groups: 1
Mapping software: JoinMap ver. 3
LOD value for grouping: 5.0
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| Map Set Name: |
Pear-Kin1b-F1 |
[ Show Only This Set ] |
| Abbreviated Name: |
Kinchaku1b |
[ Download Map Set Data ] |
| Accession ID: |
47 |
[ View Map Set In Matrix ] |
| Species: |
Pyrus (Pear) |
[ View Species Info ] |
| Map Type: |
Genetic |
[ View Map Type Info ] |
| Map Units: |
cM |
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| Published On: |
01 August, 2006 |
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Description (abstract):
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Pear scab (caused by Venturia nashicola) is one of the most harmful diseases of pears, especially Japanese and Chinese pear species. The molecular identification and early selection of resistant plants could greatly improve pear breeding. We have identified the position of the scab resistance gene, designated Vnk in an indigenous Japanese pear cultivar Kinchaku, within the pear genome by using simple sequence repeat (SSR) markers derived from pear and apple. The position of Vnk was identified in the central region of linkage group 1 of Kinchaku. Several amplified fragment length polymorphism (AFLP) markers linked to Vnk were obtained by bulked segregant analysis. Among them, the AFLP marker closest to Vnk was converted into a sequence tagged site (STS) marker. Four random amplified polymorphic DNA (RAPD) markers previously found to be loosely associated with Vnk (Iketani et al. 2001) were successfully converted into STS markers. Six markers (one SSR Hi02c07 and five STSs converted from AFLP and RAPD) showed tight linkages to Vnk, being mapped with distances ranging from 2.4 to 12.4 cM. The SSR CH-Vf2, which was isolated from a BAC clone of the contig containing the apple scab gene Vf, was mapped at the bottom of linkage group 1 in Kinchaku, suggesting that the Vnk and Vf loci are located in different genomic regions of the same homologous linkage group. |
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Description:
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Population name: Shuurei x 314-32
Cross combination: Shuurei x 314-32 (Kinchaku x Housui)
Population type: F1
Number of individuals: 112
Number of loci: 14
Number of linkage groups: 1
Mapping software: JoinMap ver. 3
LOD value for grouping: 5.0
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| Map Set Name: |
Pear-AFxMRB-F1 |
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| Abbreviated Name: |
Pear-AFxMRB-F1 |
[ Download Map Set Data ] |
| Accession ID: |
63 |
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| Species: |
Pyrus (Pear) |
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| Map Type: |
Genetic |
[ View Map Type Info ] |
| Map Units: |
cM |
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| Published On: |
10 August, 2010 |
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