| Library Name | Organism | Cultivar | Development Stage | Tissue Type | Number of ESTs | Note |
| LIBEST_020089 Paradormant buds from Rubus idaeus L. cv Glen Ample | Rubus idaeus | Glen Ample | 1200 - 2500 Chilling Units | buds | 131 | Vector: pSPORT1 |
| LIBEST_021969 Roots from Rubus strigosus | Rubus idaeus subsp. strigosus | Latham | roots | 21 | Vector: pSPORT1; Sequenced with M13 forward & reverse primers | |
| LIBEST_025071 Roots from Rubus idaeus subsp. strigosus | Rubus idaeus subsp. strigosus | Latham | Roots | 5 | Vector: pSPORT1; sequenced with M13 forward and reverse primers | |
| LIBEST_025167 Red raspberry root library | Rubus idaeus subsp. strigosus | Latham | Roots | 30 | Vector: pSPORT1; Sequenced with universal M13 reverse primer | |
| LIBEST_022999 RH_MEa blackberry emerging leaf | Rubus ulmifolius var. inermis x Rubus thyrsiger | Merton Thornless | Newly emerging | Leaf | 2678 | Vector: pDONR222; Site_1: sfiI; Site_2: sfiII; Rubus F2 backcross to F1 of R. ulmifolius (R. rusticanus) inermis x R. thyrsiger. The tetraploid blackberry cultivar, Merton Thornless (PI 553276), was selected for EST development, because it is the source of the thornless trait in many commercially grown thornless cultivars. Young expanding leaves of Merton Thornless were harvested in July, 2004, from plants growing on the North Farm of the Beltsville Agricultural Research Center. No enrichment was done of the total RNA extracted before mRNA was separated from total RNA. The cDNA library was cloned in the Gateway system (Invitrogen Corp., Carlsbad, Calif.) with the pDONR222 vector and ElectroMAX DH10B T1 Phage Resistant Cells (Invitrogen Corp.). Kanamycin was used to select colonies containing vectors. The M13F primer was used to sequence from the 5 end of 3884 clones. |
| LIBEST_020088 Endodormant buds from Rubus idaeus L. cv Glen Ample | Rubus idaeus | Glen Ample | 0 - 1200 Chilling Units | buds | 196 | Vector: pBluescript |
