Mapping with a few plants: using selective mapping for microsatellite saturation of the Prunus reference map

Publication Overview
TitleMapping with a few plants: using selective mapping for microsatellite saturation of the Prunus reference map
AuthorsHowad W, Yamamoto T, Dirlewanger E, Testolin R, Cosson P, Cipriani G, Monforte AJ, Georgi L, Abbott AG, Arús P
TypeJournal Article
Journal NameGenetics
Volume171
Issue3
Year2005
Page(s)1305-1309
CitationHowad W, Yamamoto T, Dirlewanger E, Testolin R, Cosson P, Cipriani G, Monforte AJ, Georgi L, Abbott AG, Arús P. Mapping with a few plants: using selective mapping for microsatellite saturation of the Prunus reference map. 2005 Nov; 171(3):1305-1309.

Abstract

The concept of selective (or bin) mapping is used here for the first time, using as an example the Prunus reference map constructed with an almond x peach F2 population. On the basis of this map, a set of six plants that jointly defined 65 possible different genotypes for the codominant markers mapped on it was selected. Sixty-three of these joint genotypes corresponded to a single chromosomal region (a bin) of the Prunus genome, and the two remaining corresponded to two bins each. The 67 bins defined by these six plants had a 7.8-cM average length and a maximum individual length of 24.7 cM. Using a unit of analysis composed of these six plants, their F1 hybrid parent, and one of the parents of the hybrid, we mapped 264 microsatellite (or simple-sequence repeat, SSR) markers from 401 different microsatellite primer pairs. Bin mapping proved to be a fast and economic strategy that could be used for further map saturation, the addition of valuable markers (such as those based on microsatellites or ESTs), and giving a wider scope to, and a more efficient use of, reference mapping populations.

Features
This publication contains information about 106 features:
Feature NameUniquenameType
EPPCU2005EPPCU2005genetic_marker
EPPCU2046EPPCU2046genetic_marker
EPPCU2256EPPCU2256genetic_marker
EPPCU2288EPPCU2288genetic_marker
EPPCU2407EPPCU2407genetic_marker
EPPCU2432EPPCU2432genetic_marker
EPPCU2520EPPCU2520genetic_marker
EPPCU2602EPPCU2602genetic_marker
EPPCU2673EPPCU2673genetic_marker
EPPCU2828EPPCU2828genetic_marker
EPPCU2842EPPCU2842genetic_marker
EPPCU2957EPPCU2957genetic_marker
EPPCU2979EPPCU2979genetic_marker
EPPCU3049EPPCU3049genetic_marker
EPPCU3062EPPCU3062genetic_marker
EPPCU3088EPPCU3088genetic_marker
EPPCU3090EPPCU3090genetic_marker
EPPCU3132EPPCU3132genetic_marker
EPPCU3216EPPCU3216genetic_marker
EPPCU3664EPPCU3664genetic_marker
EPPCU3766EPPCU3766genetic_marker
EPPCU3855EPPCU3855genetic_marker
EPPCU3886EPPCU3886genetic_marker
EPPCU4092EPPCU4092genetic_marker
EPPCU4205EPPCU4205genetic_marker

Pages

Featuremaps
This publication contains information about 2 maps:
Map Name
Prunus Bin Map
Prunus-TE-F2