Aligned genetic linkage maps of apple rootstock cultivar ‘JM7’ and Malus sieboldii ‘Sanashi 63’ constructed with novel EST-SSRs

Publication Overview
TitleAligned genetic linkage maps of apple rootstock cultivar ‘JM7’ and Malus sieboldii ‘Sanashi 63’ constructed with novel EST-SSRs
AuthorsMoriya S, Iwanami H, Kotoda N, Haji T, Okada K, Terakami S, Mimida N, Yamamoto T, Abe K
TypeJournal Article
Journal NameTree genetics & genomes
Volume8
Issue4
Year2012
Page(s)709-723
CitationMoriya S, Iwanami H, Kotoda N, Haji T, Okada K, Terakami S, Mimida N, Yamamoto T, Abe K. Aligned genetic linkage maps of apple rootstock cultivar ‘JM7’ and Malus sieboldii ‘Sanashi 63’ constructed with novel EST-SSRs. Tree genetics & genomes. 2012; 8(4):709-723.

Abstract

Identification of markers associated with genes of interest and quantitative trait loci (QTLs), combined with high-density genetic linkage maps, can help reduce labor and costs by enabling marker-assisted selection (MAS). In this study, a dwarfing apple rootstock cultivar ‘JM7’ (Malus prunifolia × Malus pumila ‘Malling 9’) and wild apple Malus sieboldii ‘Sanashi 63’ (section Sorbomalus) were used for constructing genetic linkage maps. Here, a species from section Sorbomalus was used for the first time as a target species in a genome-wide mapping study. We also developed and mapped 137 novel-expressed sequence tag-simple sequence repeat (EST-SSR) markers. The genetic linkage maps of ‘JM7’ and ‘Sanashi 63’ consisted of 415 and 310 loci and spanned 998.0 and 981.8 cM, respectively, comparable to the reference map of Malus × domestica ‘Discovery’. A BLASTN search revealed that all of the EST-SSR sequences used in this study exhibited very high homology to one or more previously characterized apple genome contigs. Although the most homologous contigs of 89 EST-SSRs were located within the same linkage groups (LGs) identified by mapping analysis, the other 48 EST-SSRs were aligned into contigs positioned in different LGs than those identified by mapping. When search criteria were expanded to include the five most homologous contigs of each EST-SSR, at least one of the top five contigs for 15 of these 48 EST-SSRs corresponded to the LG obtained by mapping. The maps of ‘JM7’ and ‘Sanashi 63’ may be useful for analyzing important rootstock characteristics and identifying markers for MAS.
Features
This publication contains information about 307 features:
Feature NameUniquenameType
MEST022MEST022genetic_marker
MEST023MEST023genetic_marker
MEST024MEST024genetic_marker
MEST026MEST026genetic_marker
MEST028MEST028genetic_marker
MEST029MEST029genetic_marker
MEST030MEST030genetic_marker
MEST031MEST031genetic_marker
MEST032MEST032genetic_marker
MEST033MEST033genetic_marker
MEST034MEST034genetic_marker
MEST036MEST036genetic_marker
MEST037MEST037genetic_marker
MEST038MEST038genetic_marker
MEST039MEST039genetic_marker
MEST041MEST041genetic_marker
MEST043MEST043genetic_marker
MEST044MEST044genetic_marker
MEST045MEST045genetic_marker
MEST049MEST049genetic_marker
MEST050MEST050genetic_marker
MEST052MEST052genetic_marker
MEST054MEST054genetic_marker
MEST056MEST056genetic_marker
MEST060MEST060genetic_marker

Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
Apple-JM7xS63-F1
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
JM7JM7accession
Sanashi 63Sanashi 63accession
JM7_x_Sanashi 63JM7_x_Sanashi 63population