High-density genetic linkage map construction and identification of fruit-related QTLs in pear using SNP and SSR markers

Publication Overview
TitleHigh-density genetic linkage map construction and identification of fruit-related QTLs in pear using SNP and SSR markers
AuthorsWu J, Li LT, Li M, Khan MA, Li XG, Chen H, Yin H, Zhang SL
TypeJournal Article
Journal NameJournal of experimental botany
Year2014
CitationWu J, Li LT, Li M, Khan MA, Li XG, Chen H, Yin H, Zhang SL. High-density genetic linkage map construction and identification of fruit-related QTLs in pear using SNP and SSR markers. Journal of experimental botany. 2014 Aug 16.

Abstract

Pear (Pyrus spp) is an important fruit crop, grown in all temperate regions of the world, with global production ranked after grape and apples among deciduous tree crops. A high-density linkage map is a valuable tool for fine mapping quantitative trait loci (QTL) and map-based gene cloning. In this study, we firstly constructed a high-density linkage map of pear using SNPs integrated with SSRs, developed by the rapid and robust technology of restriction-associated DNA sequencing (RADseq). The linkage map consists of 3143 SNP markers and 98 SSRs, 3241 markers in total, spanning 2243.4 cM, with an average marker distance of 0.70 cM. Anchoring SSRs were able to anchor seventeen linkage groups to their corresponding chromosomes. Based on this high-density integrated pear linkage map and two years of fruit phenotyping, a total of 32 potential QTLs for 11 traits, including length of pedicel (LFP), single fruit weight (SFW), soluble solid content (SSC), transverse diameter (TD), vertical diameter (VD), calyx status (CS), flesh colour (FC), juice content (JC), number of seeds (NS), skin colour (SC), and skin smooth (SS), were identified and positioned on the genetic map. Among them, some important fruit-related traits have for the first time been identified, such as calyx status, length of pedicel, and flesh colour, and reliable localization of QTLs were verified repeatable. This high-density linkage map of pear is a worthy reference for mapping important fruit traits, QTL identification, and comparison and combination of different genetic maps.

Projects
This publication contains information about 1 projects:
Project NameDescription
Pear-fruit_phenotype-Wu-2014
Features
This publication contains information about 3,175 features:
Feature NameUniquenameType
Pyb05_140Pyb05_140genetic_marker
Pyb05_141Pyb05_141genetic_marker
Pyb05_142Pyb05_142genetic_marker
Pyb05_143Pyb05_143genetic_marker
Pyb05_144Pyb05_144genetic_marker
Pyb05_146Pyb05_146genetic_marker
Pyb05_149Pyb05_149genetic_marker
Pyb05_154Pyb05_154genetic_marker
Pyb05_157Pyb05_157genetic_marker
Pyb05_158Pyb05_158genetic_marker
Pyb05_159Pyb05_159genetic_marker
Pyb05_160Pyb05_160genetic_marker
Pyb05_164Pyb05_164genetic_marker
Pyb05_169Pyb05_169genetic_marker
Pyb05_171Pyb05_171genetic_marker
Pyb05_173Pyb05_173genetic_marker
Pyb05_175Pyb05_175genetic_marker
Pyb05_177Pyb05_177genetic_marker
Pyb05_178Pyb05_178genetic_marker
Pyb05_180Pyb05_180genetic_marker
Pyb05_182Pyb05_182genetic_marker
Pyb05_183Pyb05_183genetic_marker
Pyb05_184Pyb05_184genetic_marker
Pyb05_185Pyb05_185genetic_marker
Pyb05_191Pyb05_191genetic_marker

Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
Pear-BD-F1-2014-genetic
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
DangshansuliDangshansuliaccession
Bayuehong_x_DangshansuliBayuehong_x_Dangshansulipopulation
BayuehongBayuehongaccession
Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PubMedPMID:25129128
AGL: USDA National Agricultural LibraryAGL:4893819