Publication Overview
TitleMicrosatellite marker development in rose and its application in tetraploid mapping
AuthorsZhang L.H., Byrne D.H., Ballard R.E., Rajapakse S.
TypeJournal Article
Journal NameJournal of the American Society for Horticultural Science
Volume131
Issue3
Year2006
Page(s)380-387
CitationZhang L.H., Byrne D.H., Ballard R.E., Rajapakse S.. Microsatellite marker development in rose and its application in tetraploid mapping. Journal of the American Society for Horticultural Science. 2006; 131(3):380-387.

Abstract

Microsatellite or simple sequence repeat (SSR) markers were developed from Rosa wichurana Crépin to combine two previously constructed tetraploid rose (Rosa hybrida L.) genetic maps. To isolate SSR-containing sequences from rose a small-insert genomic library was constructed from diploid Rosa wichurana and screened with several SSR probes. Specific primers were designed for 43 unique SSR regions, of which 30 primer pairs gave rise to clear PCR products. Seventeen SSR primer pairs (57%) produced polymorphism in the tetraploid rose 90-69 mapping family. These markers were incorporated into existing maps of the parents 86-7 and 82-1134, which were constructed primarily with AFLP markers. The current map of the male parent, amphidiploid 86-7, consists of 286 markers assigned to 14 linkage groups and covering 770 cM. The map of the female tetraploid parent, 82-1134, consists of 256 markers assigned to 20 linkage groups and covering 920 cM. Nineteen rose SSR loci were mapped on the 86-7 map and 11 on the 82-1134 map. Several homeologous linkage groups within maps were identified based on SSR markers. In addition, some of the SSR markers provided anchoring points between the two parental maps. SSR markers were also useful for joining small linkage groups. Based on shared SSR markers, consensus orders for four rose linkage groups between parental maps were generated. Microsatellite markers developed in this study will provide valuable tools for many aspects of rose research including future consolidation of diploid and tetraploid rose genetic linkage maps, genetic, phylogenetic and population analyses, cultivar identification, and marker-assisted selection.
Features
This publication contains information about 531 features:
Feature NameUniquenameType
KAAMCAC14KAAMCAC14genetic_marker
KAAMCAC13KAAMCAC13genetic_marker
KAAMCAC11KAAMCAC11genetic_marker
KAAMCAC10KAAMCAC10genetic_marker
KAAMCAC09KAAMCAC09genetic_marker
KAAMCAC07KAAMCAC07genetic_marker
KAAMCAC06KAAMCAC06genetic_marker
KAAMCAC05KAAMCAC05genetic_marker
KAAMCAC01KAAMCAC01genetic_marker
EAGGMCTG9EAGGMCTG9genetic_marker
EAGGMCTG7EAGGMCTG7genetic_marker
EAGGMCTG6EAGGMCTG6genetic_marker
EAGGMCTG40EAGGMCTG40genetic_marker
EAGGMCTG4EAGGMCTG4genetic_marker
EAGGMCTG39EAGGMCTG39genetic_marker
EAGGMCTG37EAGGMCTG37genetic_marker
EAGGMCTG35EAGGMCTG35genetic_marker
EAGGMCTG34EAGGMCTG34genetic_marker
EAGGMCTG32EAGGMCTG32genetic_marker
EAGGMCTG3EAGGMCTG3genetic_marker
EAGGMCTG28EAGGMCTG28genetic_marker
EAGGMCTG27EAGGMCTG27genetic_marker
EAGGMCTG23EAGGMCTG23genetic_marker
EAGGMCTG22EAGGMCTG22genetic_marker
EAGGMCTG20EAGGMCTG20genetic_marker

Pages

Featuremaps
This publication contains information about 2 maps:
Map Name
Rose-82-1134-F2
Rose-86-7-F2
Stocks
This publication contains information about 4 stocks:
Stock NameUniquenameType
90-6990-69accession
82-113482-1134accession
86-786-7accession
90-69_F290-69_F2population