Publication Overview
TitleMicrosatellite marker development in rose and its application in tetraploid mapping
AuthorsZhang L.H., Byrne D.H., Ballard R.E., Rajapakse S.
TypeJournal Article
Journal NameJournal of the American Society for Horticultural Science
Volume131
Issue3
Year2006
Page(s)380-387
CitationZhang L.H., Byrne D.H., Ballard R.E., Rajapakse S.. Microsatellite marker development in rose and its application in tetraploid mapping. Journal of the American Society for Horticultural Science. 2006; 131(3):380-387.

Abstract

Microsatellite or simple sequence repeat (SSR) markers were developed from Rosa wichurana Crépin to combine two previously constructed tetraploid rose (Rosa hybrida L.) genetic maps. To isolate SSR-containing sequences from rose a small-insert genomic library was constructed from diploid Rosa wichurana and screened with several SSR probes. Specific primers were designed for 43 unique SSR regions, of which 30 primer pairs gave rise to clear PCR products. Seventeen SSR primer pairs (57%) produced polymorphism in the tetraploid rose 90-69 mapping family. These markers were incorporated into existing maps of the parents 86-7 and 82-1134, which were constructed primarily with AFLP markers. The current map of the male parent, amphidiploid 86-7, consists of 286 markers assigned to 14 linkage groups and covering 770 cM. The map of the female tetraploid parent, 82-1134, consists of 256 markers assigned to 20 linkage groups and covering 920 cM. Nineteen rose SSR loci were mapped on the 86-7 map and 11 on the 82-1134 map. Several homeologous linkage groups within maps were identified based on SSR markers. In addition, some of the SSR markers provided anchoring points between the two parental maps. SSR markers were also useful for joining small linkage groups. Based on shared SSR markers, consensus orders for four rose linkage groups between parental maps were generated. Microsatellite markers developed in this study will provide valuable tools for many aspects of rose research including future consolidation of diploid and tetraploid rose genetic linkage maps, genetic, phylogenetic and population analyses, cultivar identification, and marker-assisted selection.
Features
This publication contains information about 531 features:
Feature NameUniquenameType
EAACMCAT5EAACMCAT5genetic_marker
EAACMCAT49EAACMCAT49genetic_marker
EAACMCAT48EAACMCAT48genetic_marker
EAACMCAT47EAACMCAT47genetic_marker
EAACMCAT46EAACMCAT46genetic_marker
EAACMCAT45EAACMCAT45genetic_marker
EAACMCAT44EAACMCAT44genetic_marker
EAACMCAT42EAACMCAT42genetic_marker
EAACMCAT41EAACMCAT41genetic_marker
EAACMCAT40EAACMCAT40genetic_marker
EAACMCAT4EAACMCAT4genetic_marker
EAACMCAT38EAACMCAT38genetic_marker
EAACMCAT36EAACMCAT36genetic_marker
EAACMCAT35EAACMCAT35genetic_marker
EAACMCAT33EAACMCAT33genetic_marker
EAACMCAT32EAACMCAT32genetic_marker
EAACMCAT31EAACMCAT31genetic_marker
EAACMCAT30EAACMCAT30genetic_marker
EAACMCAT3EAACMCAT3genetic_marker
EAACMCAT28EAACMCAT28genetic_marker
EAACMCAT27EAACMCAT27genetic_marker
EAACMCAT26EAACMCAT26genetic_marker
EAACMCAT25EAACMCAT25genetic_marker
EAACMCAT24EAACMCAT24genetic_marker
EAACMCAT19EAACMCAT19genetic_marker

Pages

Featuremaps
This publication contains information about 2 maps:
Map Name
Rose-82-1134-F2
Rose-86-7-F2
Stocks
This publication contains information about 4 stocks:
Stock NameUniquenameType
90-6990-69accession
82-113482-1134accession
86-786-7accession
90-69_F290-69_F2population