Publication Overview
TitleMicrosatellite marker development in rose and its application in tetraploid mapping
AuthorsZhang L.H., Byrne D.H., Ballard R.E., Rajapakse S.
TypeJournal Article
Journal NameJournal of the American Society for Horticultural Science
Volume131
Issue3
Year2006
Page(s)380-387
CitationZhang L.H., Byrne D.H., Ballard R.E., Rajapakse S.. Microsatellite marker development in rose and its application in tetraploid mapping. Journal of the American Society for Horticultural Science. 2006; 131(3):380-387.

Abstract

Microsatellite or simple sequence repeat (SSR) markers were developed from Rosa wichurana Crépin to combine two previously constructed tetraploid rose (Rosa hybrida L.) genetic maps. To isolate SSR-containing sequences from rose a small-insert genomic library was constructed from diploid Rosa wichurana and screened with several SSR probes. Specific primers were designed for 43 unique SSR regions, of which 30 primer pairs gave rise to clear PCR products. Seventeen SSR primer pairs (57%) produced polymorphism in the tetraploid rose 90-69 mapping family. These markers were incorporated into existing maps of the parents 86-7 and 82-1134, which were constructed primarily with AFLP markers. The current map of the male parent, amphidiploid 86-7, consists of 286 markers assigned to 14 linkage groups and covering 770 cM. The map of the female tetraploid parent, 82-1134, consists of 256 markers assigned to 20 linkage groups and covering 920 cM. Nineteen rose SSR loci were mapped on the 86-7 map and 11 on the 82-1134 map. Several homeologous linkage groups within maps were identified based on SSR markers. In addition, some of the SSR markers provided anchoring points between the two parental maps. SSR markers were also useful for joining small linkage groups. Based on shared SSR markers, consensus orders for four rose linkage groups between parental maps were generated. Microsatellite markers developed in this study will provide valuable tools for many aspects of rose research including future consolidation of diploid and tetraploid rose genetic linkage maps, genetic, phylogenetic and population analyses, cultivar identification, and marker-assisted selection.
Features
This publication contains information about 531 features:
Feature NameUniquenameType
KAGMCCA07KAGMCCA07genetic_marker
KAGMCCA06KAGMCCA06genetic_marker
KAGMCCA05KAGMCCA05genetic_marker
KAGMCCA02KAGMCCA02genetic_marker
KAGMCCA01KAGMCCA01genetic_marker
KAGMCAT30KAGMCAT30genetic_marker
KAGMCAT29KAGMCAT29genetic_marker
KAGMCAT28KAGMCAT28genetic_marker
KAGMCAT27KAGMCAT27genetic_marker
KAGMCAT26KAGMCAT26genetic_marker
KAGMCAT25KAGMCAT25genetic_marker
KAGMCAT24KAGMCAT24genetic_marker
KAGMCAT23KAGMCAT23genetic_marker
KAGMCAT22KAGMCAT22genetic_marker
KAGMCAT21KAGMCAT21genetic_marker
KAGMCAT19KAGMCAT19genetic_marker
KAGMCAT18KAGMCAT18genetic_marker
KAGMCAT17KAGMCAT17genetic_marker
KAGMCAT16KAGMCAT16genetic_marker
KAGMCAT15KAGMCAT15genetic_marker
KAGMCAT14KAGMCAT14genetic_marker
KAGMCAT12KAGMCAT12genetic_marker
KAGMCAT11KAGMCAT11genetic_marker
KAGMCAT10KAGMCAT10genetic_marker
KAGMCAT09KAGMCAT09genetic_marker

Pages

Featuremaps
This publication contains information about 2 maps:
Map Name
Rose-82-1134-F2
Rose-86-7-F2
Stocks
This publication contains information about 4 stocks:
Stock NameUniquenameType
90-6990-69accession
82-113482-1134accession
86-786-7accession
90-69_F290-69_F2population