Publication Overview
TitleConstruction of a High-Density Simple Sequence Repeat Consensus Genetic Map for Pear (Pyrus spp.)
AuthorsChen H, Song Y, Li L, Khan M, Korban S, Wu J, Zhang S
TypeJournal Article
Journal NamePlant Molecular Biology Reporter
Volume33
Issue2
Year2015
Page(s)316–325
CitationChen H, Song Y, Li L, Khan M, Korban S, Wu J, Zhang S. Construction of a High-Density Simple Sequence Repeat Consensus Genetic Map for Pear (Pyrus spp.). Plant Molecular Biology Reporter. 2015; 33(2):316–325.

Abstract

As an important fruit crop that is widely grown commercially in temperate regions of the world, pear (Pyrus) is a target for pursuing efficient breeding strategies. Construction of a reliable and dense genetic linkage map is critical for undertaking marker-assisted breeding. In this study, a population of 56 F1 seedlings of ‘Bayuehong’ × ‘Dangshansuli’ was used to construct a high-density simple sequence repeat (SSR)-based genetic linkage map. A total of 1,756 SSR markers, including 1,341 newly designed SSRs based on whole-genome sequencing of an Asiatic pear along with 415 previously reported SSRs, were first evaluated for polymorphism. Based on 894 SSRs demonstrating polymorphism, a consensus genetic map consisting of 734 loci distributed along all 17 linkage groups (LG) was constructed, with a total length of 1,661.4 cM and with an average marker interval of 2.26 cM. Comparisons among different maps of pear and apple were then made based on positions of previously mapped SSR markers on the consensus map. As a result, homologous linkage groups LG3 and LG11, LG5 and LG10, LG9 and LG17, LG13 and LG16, LG8 and LG15 have been identified. This high-density SSR map along with a set of SSR markers covering the whole genome of pear will greatly facilitate integration of independent maps, aid in pursuing comparative genome studies, and in evaluation of different germplasm in future genetic and breeding studies.
Features
This publication contains information about 437 features:
Feature NameUniquenameType
NAUpy96xNAUpy96xgenetic_marker
NAUpy97aNAUpy97agenetic_marker
NAUpy97bNAUpy97bgenetic_marker
NAUpy97iNAUpy97igenetic_marker
NAUpy97rNAUpy97rgenetic_marker
NAUpy97xNAUpy97xgenetic_marker
NAUpy98dNAUpy98dgenetic_marker
NAUpy98fNAUpy98fgenetic_marker
NAUpy98nNAUpy98ngenetic_marker
NAUpy99aNAUpy99agenetic_marker
NAUpy99fNAUpy99fgenetic_marker
NAUpy99rNAUpy99rgenetic_marker

Pages

Featuremaps
This publication contains information about 5 maps:
Map Name
Pear-BD-F1-2015
Pear-Bayuehong-F1-2015
Pear-Dangshansuli-F1-2015
Pear-Bartlett-F1-2007
Pear-La_France-F1-2007
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
Bayuehong_x_DangshansuliBayuehong_x_Dangshansulipopulation
DangshansuliDangshansuliaccession
BayuehongBayuehongaccession