A genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica

Publication Overview
TitleA genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica
AuthorsBushakra JM, Bryant DW, Dossett M, Vining KJ, VanBuren R, Gilmore BS, Lee J, Mockler TC, Finn CE, Bassil NV
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Volume128
Year2015
Page(s)1631-1646
CitationBushakra JM, Bryant DW, Dossett M, Vining KJ, VanBuren R, Gilmore BS, Lee J, Mockler TC, Finn CE, Bassil NV. A genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica. TAG. Theoretical and applied genetics. (2015) 128:1631-1646

Abstract

KEY MESSAGE
We have constructed a densely populated, saturated genetic linkage map of black raspberry and successfully placed a locus for aphid resistance. Black raspberry (Rubus occidentalis L.) is a high-value crop in the Pacific Northwest of North America with an international marketplace. Few genetic resources are readily available and little improvement has been achieved through breeding efforts to address production challenges involved in growing this crop. Contributing to its lack of improvement is low genetic diversity in elite cultivars and an untapped reservoir of genetic diversity from wild germplasm. In the Pacific Northwest, where most production is centered, the current standard commercial cultivar is highly susceptible to the aphid Amphorophora agathonica Hottes, which is a vector for the Raspberry mosaic virus complex. Infection with the virus complex leads to a rapid decline in plant health resulting in field replacement after only 3-4 growing seasons. Sources of aphid resistance have been identified in wild germplasm and are used to develop mapping populations to study the inheritance of these valuable traits. We have constructed a genetic linkage map using single-nucleotide polymorphism and transferable (primarily simple sequence repeat) markers for F1 population ORUS 4305 consisting of 115 progeny that segregate for aphid resistance. Our linkage map of seven linkage groups representing the seven haploid chromosomes of black raspberry consists of 274 markers on the maternal map and 292 markers on the paternal map including a morphological locus for aphid resistance. This is the first linkage map of black raspberry and will aid in developing markers for marker-assisted breeding, comparative mapping with other Rubus species, and enhancing the black raspberry genome assembly.

Features
This publication contains information about 442 features:
Feature NameUniquenameType
RO_S495_85717RO_S495_85717genetic_marker
RO_S496_44208RO_S496_44208genetic_marker
RO_S5_481465RO_S5_481465genetic_marker
RO_S5_672048RO_S5_672048genetic_marker
RO_S500_45749RO_S500_45749genetic_marker
RO_S500_53440RO_S500_53440genetic_marker
RO_S502_126385RO_S502_126385genetic_marker
RO_S505_61312RO_S505_61312genetic_marker
RO_S509_84964RO_S509_84964genetic_marker
RO_S51_580471RO_S51_580471genetic_marker
RO_S52_429297RO_S52_429297genetic_marker
RO_S525_75530RO_S525_75530genetic_marker
RO_S529_27629RO_S529_27629genetic_marker
RO_S53_419253RO_S53_419253genetic_marker
RO_S530_106983RO_S530_106983genetic_marker
RO_S536_107187RO_S536_107187genetic_marker
RO_S537_5881RO_S537_5881genetic_marker
RO_S537_5882RO_S537_5882genetic_marker
RO_S540_84061RO_S540_84061genetic_marker
RO_S544_70128RO_S544_70128genetic_marker
RO_S547_52503RO_S547_52503genetic_marker
RO_S548_89207RO_S548_89207genetic_marker
RO_S55_479636RO_S55_479636genetic_marker
RO_S563_15061RO_S563_15061genetic_marker
RO_S565_20930RO_S565_20930genetic_marker

Pages

Featuremaps
This publication contains information about 3 maps:
Map Name
Black_Raspberry-ORUS3021-2-F1
Black_Raspberry-ORUS4153-1-F1
Black_Raspberry-O3xO4-F1
Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
JewelJewelaccession
NC84-10-3NC84-10-3accession
ORUS3778-1ORUS3778-1accession
ORUS3021-2ORUS3021-2accession
ORUS4153-1ORUS4153-1accession
ORUS3021-2_x_ORUS4153-1ORUS3021-2_x_ORUS4153-1population