A genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica

Publication Overview
TitleA genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica
AuthorsBushakra JM, Bryant DW, Dossett M, Vining KJ, VanBuren R, Gilmore BS, Lee J, Mockler TC, Finn CE, Bassil NV
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Volume128
Year2015
Page(s)1631-1646
CitationBushakra JM, Bryant DW, Dossett M, Vining KJ, VanBuren R, Gilmore BS, Lee J, Mockler TC, Finn CE, Bassil NV. A genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica. TAG. Theoretical and applied genetics. (2015) 128:1631-1646

Abstract

KEY MESSAGE
We have constructed a densely populated, saturated genetic linkage map of black raspberry and successfully placed a locus for aphid resistance. Black raspberry (Rubus occidentalis L.) is a high-value crop in the Pacific Northwest of North America with an international marketplace. Few genetic resources are readily available and little improvement has been achieved through breeding efforts to address production challenges involved in growing this crop. Contributing to its lack of improvement is low genetic diversity in elite cultivars and an untapped reservoir of genetic diversity from wild germplasm. In the Pacific Northwest, where most production is centered, the current standard commercial cultivar is highly susceptible to the aphid Amphorophora agathonica Hottes, which is a vector for the Raspberry mosaic virus complex. Infection with the virus complex leads to a rapid decline in plant health resulting in field replacement after only 3-4 growing seasons. Sources of aphid resistance have been identified in wild germplasm and are used to develop mapping populations to study the inheritance of these valuable traits. We have constructed a genetic linkage map using single-nucleotide polymorphism and transferable (primarily simple sequence repeat) markers for F1 population ORUS 4305 consisting of 115 progeny that segregate for aphid resistance. Our linkage map of seven linkage groups representing the seven haploid chromosomes of black raspberry consists of 274 markers on the maternal map and 292 markers on the paternal map including a morphological locus for aphid resistance. This is the first linkage map of black raspberry and will aid in developing markers for marker-assisted breeding, comparative mapping with other Rubus species, and enhancing the black raspberry genome assembly.

Features
This publication contains information about 442 features:
Feature NameUniquenameType
RO_S577_10160RO_S577_10160genetic_marker
RO_S59_190044RO_S59_190044genetic_marker
RO_S590_9650RO_S590_9650genetic_marker
RO_S595_84360RO_S595_84360genetic_marker
RO_S6_272056RO_S6_272056genetic_marker
RO_S60_604050RO_S60_604050genetic_marker
RO_S604_66577RO_S604_66577genetic_marker
RO_S608_26380RO_S608_26380genetic_marker
RO_S609_54570RO_S609_54570genetic_marker
RO_S61_295208RO_S61_295208genetic_marker
RO_S61_40795RO_S61_40795genetic_marker
RO_S612_66375RO_S612_66375genetic_marker
RO_S615_66181RO_S615_66181genetic_marker
RO_S62_439154RO_S62_439154genetic_marker
RO_S6272056RO_S6272056genetic_marker
RO_S63_573175RO_S63_573175genetic_marker
RO_S630_40278RO_S630_40278genetic_marker
RO_S631_78691RO_S631_78691genetic_marker
RO_S64_165666RO_S64_165666genetic_marker
RO_S648_18748RO_S648_18748genetic_marker
RO_S65_461926RO_S65_461926genetic_marker
RO_S650_32526RO_S650_32526genetic_marker
RO_S651_61319RO_S651_61319genetic_marker
RO_S653_21318RO_S653_21318genetic_marker
RO_S654_42261RO_S654_42261genetic_marker

Pages

Featuremaps
This publication contains information about 3 maps:
Map Name
Black_Raspberry-ORUS3021-2-F1
Black_Raspberry-ORUS4153-1-F1
Black_Raspberry-O3xO4-F1
Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
JewelJewelaccession
NC84-10-3NC84-10-3accession
ORUS3778-1ORUS3778-1accession
ORUS3021-2ORUS3021-2accession
ORUS4153-1ORUS4153-1accession
ORUS3021-2_x_ORUS4153-1ORUS3021-2_x_ORUS4153-1population