A genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica

Publication Overview
TitleA genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica
AuthorsBushakra JM, Bryant DW, Dossett M, Vining KJ, VanBuren R, Gilmore BS, Lee J, Mockler TC, Finn CE, Bassil NV
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Volume128
Year2015
Page(s)1631-1646
CitationBushakra JM, Bryant DW, Dossett M, Vining KJ, VanBuren R, Gilmore BS, Lee J, Mockler TC, Finn CE, Bassil NV. A genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica. TAG. Theoretical and applied genetics. (2015) 128:1631-1646

Abstract

KEY MESSAGE
We have constructed a densely populated, saturated genetic linkage map of black raspberry and successfully placed a locus for aphid resistance. Black raspberry (Rubus occidentalis L.) is a high-value crop in the Pacific Northwest of North America with an international marketplace. Few genetic resources are readily available and little improvement has been achieved through breeding efforts to address production challenges involved in growing this crop. Contributing to its lack of improvement is low genetic diversity in elite cultivars and an untapped reservoir of genetic diversity from wild germplasm. In the Pacific Northwest, where most production is centered, the current standard commercial cultivar is highly susceptible to the aphid Amphorophora agathonica Hottes, which is a vector for the Raspberry mosaic virus complex. Infection with the virus complex leads to a rapid decline in plant health resulting in field replacement after only 3-4 growing seasons. Sources of aphid resistance have been identified in wild germplasm and are used to develop mapping populations to study the inheritance of these valuable traits. We have constructed a genetic linkage map using single-nucleotide polymorphism and transferable (primarily simple sequence repeat) markers for F1 population ORUS 4305 consisting of 115 progeny that segregate for aphid resistance. Our linkage map of seven linkage groups representing the seven haploid chromosomes of black raspberry consists of 274 markers on the maternal map and 292 markers on the paternal map including a morphological locus for aphid resistance. This is the first linkage map of black raspberry and will aid in developing markers for marker-assisted breeding, comparative mapping with other Rubus species, and enhancing the black raspberry genome assembly.

Features
This publication contains information about 442 features:
Feature NameUniquenameType
RO_S122_230445RO_S122_230445genetic_marker
RO_S122_74787RO_S122_74787genetic_marker
RO_S124_714RO_S124_714genetic_marker
RO_S127_265724RO_S127_265724genetic_marker
RO_S129_153488RO_S129_153488genetic_marker
RO_S13_940743RO_S13_940743genetic_marker
RO_S1313_1723RO_S1313_1723genetic_marker
RO_S134_217734RO_S134_217734genetic_marker
RO_S134_333655RO_S134_333655genetic_marker
RO_S134_395808RO_S134_395808genetic_marker
RO_S134_74067RO_S134_74067genetic_marker
RO_S134_83955RO_S134_83955genetic_marker
RO_S135_1836RO_S135_1836genetic_marker
RO_S136_346904RO_S136_346904genetic_marker
RO_S138_172691RO_S138_172691genetic_marker
RO_S139_144731RO_S139_144731genetic_marker
RO_S139_162862RO_S139_162862genetic_marker
RO_S1391_6231RO_S1391_6231genetic_marker
RO_S14_44081RO_S14_44081genetic_marker
RO_S14_877960RO_S14_877960genetic_marker
RO_S14_952525RO_S14_952525genetic_marker
RO_S140_40577RO_S140_40577genetic_marker
RO_S142_215410RO_S142_215410genetic_marker
RO_S142_375833RO_S142_375833genetic_marker
RO_S142_7482RO_S142_7482genetic_marker

Pages

Featuremaps
This publication contains information about 3 maps:
Map Name
Black_Raspberry-ORUS3021-2-F1
Black_Raspberry-ORUS4153-1-F1
Black_Raspberry-O3xO4-F1
Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
JewelJewelaccession
NC84-10-3NC84-10-3accession
ORUS3778-1ORUS3778-1accession
ORUS3021-2ORUS3021-2accession
ORUS4153-1ORUS4153-1accession
ORUS3021-2_x_ORUS4153-1ORUS3021-2_x_ORUS4153-1population