Publication Overview
Abstract Simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers were evaluated in an
effort to reliably DNA fingerprint sweet cherry (Prunus avium L.) cultivars and advanced selections from the breeding
program at the Summerland Research and Development Center (Summerland, BC, Canada). SSR markers were
found that differentiated the 35 cultivars and selections tested. However, groups of cultivars closely related to the
parental cultivars, Lapins and Sweetheart, were differentiated by only a few SSR markers each. These last few
markers were discovered by specifically screening within these small groups of cultivars and the resulting markers
had lower discriminating power (Dj) statistics within the full set of 35 cultivars and selections. To further characterize
the differences in one of these closely related groups, SNP markers were identified in the cultivar Sweetheart and an
analysis was made of how these markers segregated into three of its open-pollinated progeny. Large blocks of the
‘Sweetheart’ genome (34%) did not contain informative SNP markers, which was consistent with its ancestry where
the cultivar Van is both a parent and grandparent. The three progeny cultivars differed from ‘Sweetheart’ at 14%,
31%, and 29% of the 3011 SNP positions tested. These were located in blocks of linked haplotypes covering from 2.5 to
20 million bps each and were distinct for the three cultivars. The cultivar Staccato, which required the most effort for
SSR marker discrimination, also had the lowest number of SNP position differences from ‘Sweetheart’ (14%). These
informative SNP markers were located in only five small regions of the sweet cherry genome, which also contained the
discriminating SSR markers and provides an explanation for the difficulty of locating SSR markers for this cultivar.
In addition to clearly differentiating these cultivars, this SNP analysis shows the level of variation expected within this
closely related group.
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