ARSFL-092 (genetic_marker) Fragaria ananassa | GDR

Marker Overview

Marker Overview


Name	ARSFL-092
Genbank ID	N/A
Type	SSR
Species	Fragaria x ananassa
Germplasm	Earliglow
Source Type	genomic clone
Repeat Motif	CT8&GA13
PCR Condition	Final reaction volume of 12.5ul comprising 1.5ul template DNA, 1x PCR buffer, 1.5mM Mg2+, 200 M dNTPs, 0.2uM each primer and 0.25U Taq polymerase. PCR cycles: initial denaturation step of 94oC (2min), then 10 cycles of 94oC (30s), 55-50oC annealing temperature decreasing by 0.5oC per cycle (45s) and 72oC (1min), followed by 25 cycles of 94oC (30s), 50oC (45s) and 72oC (1min), and a final elongation step of 72oC (5min)
Primer 1	ARSFL-092.ARSFL-092F: TCCGGTGACGAATCTAAAGG
Primer 2	ARSFL-092.ARSFL-092R: GAAGAACAAGCACCACCACA
Publication	[view all]
Contact	D. Sargent

Contact

Name

Details

D. Sargent

First name:	Dan
Last name:	Sargent
Institution:	East Malling Research
Address:	New Road, East Malling, Kent ME19 6BJ, UK
Email:	dan.sargent@emr.ac.uk
Phone:	+44 1732 523747

Publications


Year	Publication
2006	Nier S, Simpson DW, Tobutt KR, Sargent DJ. A genetic linkage map of an inter-specific diploid Fragaria BC1 mapping population and its comparison with the Fragaria reference map (FV x FN). Journal of Horticultural Science and Biotechnology. 2006; 81(4):645-650.
2006	Molecular ecology notes. Oxford, UK : Blackwell Science Ltd 2006 Mar., v. 6, no. 1 p. 197-200. DUM0003559ISSN 1471-8278

Germplasm


Stock Name	Type
Earliglow	accession

Map Positions


#	Map Name	Linkage Group	Bin	Position	Locus	MapViewer
1	Fragaria-815x903-BC1	I	N/A	33.47	ARSFL-092	View