CH02D12 (genetic_marker) Malus domestica

Marker Overview
NameCH02D12
Genbank IDN/A
TypeSSR
SpeciesMalus x domestica
Repeat Motif(GA)19
PCR ConditionPCR in 15 ul total volume with 10-50 ng DNA template and 0.3 to 0.7 pM of primers combined with 1.5 unites Taq Polymerase (Promega, Madison, Wis.), 1x Promega magnesium free buffer, 0.25 mM MgCl2, adn 0.25 mM dNTP (Promega). PCR amplifications were carried out using a PTC200 thermocycler (MJ Research, Reno, Nev.). The PCR proram had an initial denaturation step of 2 min at 95C followed by 30 cycles of 30 s at 95C, 30 s at 58C, 15 s at 72 C adn ending with a final extension step of 2 min at 72C. PCR rxn were diluted 1:1 in 95% formamide, 50 mM EDTA bromophenol blue loading dye, adn denatured at 95C for 3 min.
94 °C for 5 min, 35x (94 °C for 60 s, 55 °C for 60 s, 72 °C for 60 s), 72°C for 10 min
Primer 1CH02D12.F: AACCAGATTTGCTTGCCATC
Primer 2CH02D12.R: GCTGGTGGTAAACGTGGTG
Primer 3CH02d12-Forward: AACCAGATTTGCTTGCCATC
Primer 4CH02d12-Reverse: GCTGGTGGTAAACGTGGTG
Product Length177-199
244-245
Max Length245
PolymorphismP_ CH02D12
Publication[view all]
ContactC. Gessler
Miyuki Kunihisa
Contact
NameDetails
C. Gessler
First name:Cesare
Last name:Gessler
Institution:ETH Zurich
Address:ZTH Zurich Institut f. Integrative Biologie LFW C 15 Universitatstrasse 2 8092 Zurich
Country:Switzerland
Email:cesare.gessler@agrl.ethz.ch
Phone:+41 44 632 38 71
Fax:+41 (0) 632 11 08
Last update:May 2002
Miyuki Kunihisa
First name:Miyuki
Last name:Kunihisa
Institution:NARO Institute of Fruit Tree Science
Address:2-1 Fujimoto, Tsukuba, Ibaraki 305-8605
Country:Japan
Email:miyuky@affrc.go.jp
Phone:81-29-838-6437
Keywords:fruit drop
Map Positions
#Map NameLinkage GroupBinPositionLocusMapViewer
1Apple-SG-F111N/A26.9CH02d12_SGView
2Apple-FD-F1-2006F11N/A23.6CH02d12View
3Apple-PF-F1-2012ch11N/A21CH02d12View
4Apple-OA-F1-OrinOR11N/A28.9CH02d12View
5Apple-GDxJ-F1-2012LG11N/A63.7CH02d12View
6Apple-JGD-F1-2014J11N/A7.4CH02d12View
7Apple-JGD-F1-2014G11N/A5.8CH02d12View
8Apple-RGTxGD-F1-2015GD_11N/A33.48CH02d12View
9Pear-BD-F1-2015LG11N/A57.9CH02D12View
10Pear-Bayuehong-F1-2015LG11N/A58.02CH02D12View
11Pear-integrated_consensus_map-IPCG-2017LG11N/A154.7CH02D12View
12Apple-JM7xS63-F1J11N/A21.7CH02D12View
13Apple-JM7xS63-F1S11N/A17CH02D12View