|
|
Marker Overview
| Name | EMFn136 |
|---|
| Genbank ID | AM051332 |
|---|
| Type | SSR |
|---|
| Species | Fragaria nubicola |
|---|
| Germplasm | EMR-601 |
|---|
| Source Type | genomic clone |
|---|
| Repeat Motif | CT14
Direct |
|---|
| PCR Condition | Final reaction volume of 12.5ul comprising 1.5ul template DNA, 1x PCR buffer, 1.5mM Mg2+, 200 M dNTPs, 0.2uM each primer and 0.25U Taq polymerase. PCR cycles: initial denaturation step of 94oC (2min), then 10 cycles of 94oC (30s), 55-50oC annealing temperature decreasing by 0.5oC per cycle (45s) and 72oC (1min), followed by 25 cycles of 94oC (30s), 50oC (45s) and 72oC (1min), and a final elongation step of 72oC (5min) |
|---|
| Primer 1 | EMFn136.EMFn136F: TTTCTCTTTTGCTCCATAGTTCC |
|---|
| Primer 2 | EMFn136.EMFn136R: TTCATCAGGATCCAGAAGTCC |
|---|
| Primer 3 | EMFn136.Forward: TTTCTCTTTTGCTCCATAGTTCC |
|---|
| Primer 4 | EMFn136.Reverse (no gttt): TTCATCAGGATCCAGAAGTCC |
|---|
| Publication | [view all] |
|---|
| Contact | D. Sargent
Eric van de Weg
|
|---|
Publications
| Year | Publication |
|---|
| 2006 | Nier S, Simpson DW, Tobutt KR, Sargent DJ. A genetic linkage map of an inter-specific diploid Fragaria BC1 mapping population and its comparison with the Fragaria reference map (FV x FN). Journal of Horticultural Science and Biotechnology. 2006; 81(4):645-650. |
| 2006 | Sargent DJ, Clarke J, Simpson DW, Tobutt KR, Arús P, Monfort A, Vilanova S, Denoyes-Rothan B, Rousseau M, Folta KM, Bassil NV, Battey NH. An enhanced microsatellite map of diploid Fragaria. Theoretical and Applied Genetics. 2006 May; 112(7):1349-1359. |
Sequence
>EMFn136 ID=EMFn136; Name=EMFn136; organism=Fragaria nubicola; type=genetic_marker; length=242bp
TTTCTCTTTTGCTCCATAGTTCCCCAGAACTGGACTGGTTCTATTTAGTT
CTCTCTCTCTCTCTCTCTCTCTCTCTCTTTGCTATATGTGTCGGTGGTTG
AGCTAATCTGAATTTGTGACTTACCCCTGTTTCTTTTCTTGTTTCCTGAT
CCAAAAGCAGTTCTTATCTTCTGCTTTTCATAATTTAAAGATGTAAACTT
ATCTTTTGATTTATGATTTAGGGACTTCTGGATCCTGATGAA
|
