EMFn207 (genetic_marker) Fragaria nubicola

Marker Overview
NameEMFn207
Genbank IDAM051346
TypeSSR
SpeciesFragaria nubicola
GermplasmEMR-601
Source Typegenomic clone
Repeat MotifAG12
Direct
PCR ConditionFinal reaction volume of 12.5ul comprising 1.5ul template DNA, 1x PCR buffer, 1.5mM Mg2+, 200 M dNTPs, 0.2uM each primer and 0.25U Taq polymerase. PCR cycles: initial denaturation step of 94oC (2min), then 10 cycles of 94oC (30s), 55-50oC annealing temperature decreasing by 0.5oC per cycle (45s) and 72oC (1min), followed by 25 cycles of 94oC (30s), 50oC (45s) and 72oC (1min), and a final elongation step of 72oC (5min)
Primer 1EMFn207.EMFn207F: TTGGCAAGAATTTATAGCATCG
Primer 2EMFn207.EMFn207R: TCAGGATGTCTTCAGCAAGG
Primer 3EMFn207.Forward: TTGGCAAGAATTTATAGCATCG
Primer 4EMFn207.Reverse (no gttt): TCAGGATGTCTTCAGCAAGG
Publication[view all]
ContactD. Sargent
Eric van de Weg
Contact
NameDetails
D. Sargent
First name:Dan
Last name:Sargent
Institution:East Malling Research
Address:New Road, East Malling, Kent ME19 6BJ, UK
Email:dan.sargent@emr.ac.uk
Phone:+44 1732 523747
Eric van de Weg
First name:Eric
Last name:van de Weg
Title:researcher
Institution:Wageningen-UR Plant Breeding, Wageningen University and Research Centre
Address:P.O. Box 16, 6700 AA Wageningen, The Netherlands
Country:The Netherlands
Email:eric.vandeweg@wur.nl
Keywords:allo-octoploid strawberry
Germplasm
Stock NameType
EMR-601accession
Map Positions
#Map NameLinkage GroupBinPositionLocusMapViewer
1Fragaria-815x903-BC1IIIN/A49.11EMFn207View
2Fragaria-FVxFN-F2-2010FG3N/A43.92EMFn207View
3Strawberry-HK-F1LG3DN/A61EMFn207View
4Strawberry-HK-F1LG3C_HN/A73.2EMFn207View
5Strawberry-HK-F1LG3C_KN/A73.2EMFn207View
6Strawberry-High_Density_SSR_Map-F13BN/A76.46EMFn207View
7Strawberry-RVxFB-NIL-2017LG3N/A58.1EMFn207View
8Strawberry-Reikou-S13BIIIN/A56.49EMFn207aView
9Strawberry-Reikou-S13BIIIN/A56.49EMFn207bView
Sequence
>EMFn207 ID=EMFn207; Name=EMFn207; organism=Fragaria nubicola; type=genetic_marker; length=239bp
TTGGCAAGAATTTATAGCATCGTGCAGTAAGAAAATACAGATATAGGAAT
TAACGGGAGAAAGAAGAACCTTCAAGAAGCGGCCCCTGCGGTTCTCGCCG
ATATCAAAGTAGAAGACCTGAAGACAAAGGCGAGAAAAGTAAAGATCATG
AGCAGAGAGAGAGAGAGAGAGAGAGAGGGCAGAGAGAGAGAGAGAGTGCC
TTGGTGTCGAGCTGCAATTCCTTGCTGAAGACATCCTGA