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Marker Overview
Name | EMFvi146 |
Genbank ID | AJ564351 |
Type | SSR |
Species | Fragaria viridis |
Germplasm | EMR-903 |
Source Type | genomic clone |
Repeat Motif | AC 17mer |
PCR Condition | Final reaction volume of 12.5ul comprising 1.5ul template DNA, 1x PCR buffer, 1.5mM Mg2+, 200 M dNTPs, 0.2uM each primer and 0.25U Taq polymerase. PCR cycles: initial denaturation step of 94oC (2min), then 10 cycles of 94oC (30s), 55-50oC annealing temperature decreasing by 0.5oC per cycle (45s) and 72oC (1min), followed by 25 cycles of 94oC (30s), 50oC (45s) and 72oC (1min), and a final elongation step of 72oC (5min) |
Primer 1 | EMFvi146.EMFvi146F: ACGAAAAACAGCATACACATCG |
Primer 2 | EMFvi146.EMFvi146R: TACCGCCCCTTAAGTTTGAGTA |
Primer 3 | EMFvi146.Forward: ACGAAAAACAGCATACACATCG |
Primer 4 | EMFvi146.Reverse (no gttt): TACCGCCCCTTAAGTTTGAGTA |
Publication | [view all] |
Contact | D. Sargent Eric van de Weg
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Sequence
>EMFvi146 ID=EMFvi146; Name=EMFvi146; organism=Fragaria viridis; type=genetic_marker; length=196bp ACGAAAAACAGCATACACATCGCGATAAACTCTTGAATGACTCTACTTAA CAATGTCGTCATTCTCTTCCATGATGCTAACTCTTCTCAAAAATATCAAT ATCTACACACACACACACACATTCATTTTCTCTTCCTTTTCGTATTACTT TTCGTCAACTCGCTTCTACATTTTTACTCAAACTTAAGGGGCGGTA
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