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Marker Overview
| Name | EMFvi166 |
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| Genbank ID | AJ564352 |
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| Type | SSR |
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| Species | Fragaria viridis |
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| Germplasm | EMR-903 |
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| Source Type | genomic clone |
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| Repeat Motif | AG |
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| PCR Condition | Final reaction volume of 12.5ul comprising 1.5ul template DNA, 1x PCR buffer, 1.5mM Mg2+, 200 M dNTPs, 0.2uM each primer and 0.25U Taq polymerase. PCR cycles: initial denaturation step of 94oC (2min), then 10 cycles of 94oC (30s), 55-50oC annealing temperature decreasing by 0.5oC per cycle (45s) and 72oC (1min), followed by 25 cycles of 94oC (30s), 50oC (45s) and 72oC (1min), and a final elongation step of 72oC (5min) |
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| Primer 1 | EMFvi166.EMFvi166F: ACCGACAGCTGAGTTAGAGGAG |
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| Primer 2 | EMFvi166.EMFvi166R: AGTCATAGGACCCCACTTCAAA |
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| Publication | [view all] |
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| Contact | D. Sargent
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| Comment | c: present in one parent only (1:1 segregation), h: heterozyogous (1:3 segregation), d: distorted c markers, y:distorted h markers |
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Sequence
>EMFvi166 ID=EMFvi166; Name=EMFvi166; organism=Fragaria viridis; type=genetic_marker; length=269bp
ACCGACAGCTGAGTTAGAGGAGGGGTCAAAGCCTTAAAGGTTAAAGCTTT
AATGGAGTTGGAGTAGAATAGAGAGAGAGAGAGAGAGAGAGAAACCCTAG
CTTTTATTTGCAGGGAGAGCTCGTGAAAAAGAAGTTGAAAAGTTGAGAAA
AGGGGGAGGAGGAAGAAGAAGAAGAGGGAGGAGGGACTAGTTGGGCTTTG
GTTTTGGCTTTGTCACTTTGTAGATTGGGGTTTAGATTCTTTGAGCTTTT
GAAGTGGGGTCCTATGACT
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