GD103 (genetic_marker) Malus domestica

Marker Overview
NameGD103
Genbank IDN/A
TypeSSR
SpeciesMalus x domestica
Repeat Motif(GA)20
PCR ConditionTwo multiplexed primer sets, GD 12, 15 and 96 and GD100 and 103, were amplified in 25-microliter mixtures containing 25 ng of genomic DNA, 10-45 pmol of each primer, 0.20 mM of dNTPs, 2.0 mM MgSO4, 1 X buffer, and 0.25 units of polymerase. The multiplexed primer set GD142, 147 and 162 was amplified in a 20-microliter mixture containing 30 ng of genomic DNA, 20 pmols of each primer pair, same as above for dNTPs and MgSO4, 1 X buffer and 0.20 units polymerase. Multiplex primer set GD 142, 147 and 162 was amplified using a touchdown. Initially, 2 min at 94 degree followed by 2 cycles of 94C for 60 s, 65C 30 s, and 72C for 45s. The following 18 cycles had an annealing temp reduced by 1 degree per 2 cycles. The last 5 cycles maintained the 55C annealing temperature. The multiplex primer sets GD 12, GD 15, GD 96 and GD 100 and GD 103 were amplified following a 4-min denaturation at 94C . Reaction conditions were 25 cycles at 94C for 60s, 55C for 120s, 72C for 120s and a 10m extension at 72C.
Primer 1GD103.F: CGGCGAGAAAAAAAAACAATG
Primer 2GD103.R: GGATAACCGTCCCCCTCTTC
Product Length90-133
Max Length133
Publication[view all]
ContactS. Hokanson
Contact
NameDetails
S. Hokanson
First name:Stan
Last name:Hokanson
Institution:University of Minnesota
Address:Department of Horticultural Science, 1970 Folwell Avenue, St. Paul, MN 55108
Country:United States
Email:hokan017@umn.edu
Phone:16126241203
Fax:(301)504-5062
Last update:Apr 1998
Relationships

This genetic_marker is adjacent to the following primer feature(s):

Feature NameUnique NameSpeciesType
FGD103.FMalus x domesticaprimer
RGD103.RMalus x domesticaprimer


The following marker_locus feature(s) are an instance of this genetic_marker:

Feature NameUnique NameSpeciesType
GD103GD103Malus x domesticamarker_locus
GD103.zGD103.zMalus x domesticamarker_locus
GD103GD103-38.4Malus x domesticamarker_locus
GD103GD103-4.5Malus x domesticamarker_locus
GD103GD103-8.3Malus x domesticamarker_locus
GD103GD103-3.4Malus x domesticamarker_locus


Map Positions
#Map NameLinkage GroupBinPositionLocusMapViewer
1Apple-2000-2012-F1ch5N/A70GD103View
2Apple-PF-F1-2012ch5N/A68GD103View
3Apple-GDxJ-F1-2012LG5N/A88GD103View
4Apple-JGD-F1-2014J5N/A82.3GD103View
5Apple-RGxPI6-F1LG5N/A78GD103View
6Apple-MM-F15N/A54.3GD103.zView
7Apple-M432-2012LG5N/A49.9GD103.zView
8Apple-M27xM116-2016LG5N/A54.5GD103.zView
9Apple-JM7xS63-F1J4N/A38.4GD103View
10Apple-JM7xS63-F1S4N/A4.5GD103View
11Apple-JM7xS63-F1J14N/A8.3GD103View
12Apple-JM7xS63-F1S14N/A3.4GD103View