Mapping with a few plants: using selective mapping for microsatellite saturation of the Prunus reference map

Publication Overview
TitleMapping with a few plants: using selective mapping for microsatellite saturation of the Prunus reference map
AuthorsHowad W, Yamamoto T, Dirlewanger E, Testolin R, Cosson P, Cipriani G, Monforte AJ, Georgi L, Abbott AG, Arús P
TypeJournal Article
Journal NameGenetics
Volume171
Issue3
Year2005
Page(s)1305-1309
CitationHowad W, Yamamoto T, Dirlewanger E, Testolin R, Cosson P, Cipriani G, Monforte AJ, Georgi L, Abbott AG, Arús P. Mapping with a few plants: using selective mapping for microsatellite saturation of the Prunus reference map. 2005 Nov; 171(3):1305-1309.

Abstract

The concept of selective (or bin) mapping is used here for the first time, using as an example the Prunus reference map constructed with an almond x peach F2 population. On the basis of this map, a set of six plants that jointly defined 65 possible different genotypes for the codominant markers mapped on it was selected. Sixty-three of these joint genotypes corresponded to a single chromosomal region (a bin) of the Prunus genome, and the two remaining corresponded to two bins each. The 67 bins defined by these six plants had a 7.8-cM average length and a maximum individual length of 24.7 cM. Using a unit of analysis composed of these six plants, their F1 hybrid parent, and one of the parents of the hybrid, we mapped 264 microsatellite (or simple-sequence repeat, SSR) markers from 401 different microsatellite primer pairs. Bin mapping proved to be a fast and economic strategy that could be used for further map saturation, the addition of valuable markers (such as those based on microsatellites or ESTs), and giving a wider scope to, and a more efficient use of, reference mapping populations.

Features
This publication contains information about 106 features:
Feature NameUniquenameType
aprigms18aprigms18genetic_marker
aprigms24aprigms24genetic_marker
EPPCU0004EPPCU0004genetic_marker
EPPCU0027EPPCU0027genetic_marker
EPPCU0053EPPCU0053genetic_marker
EPPCU0443EPPCU0443genetic_marker
EPPCU0445EPPCU0445genetic_marker
EPPCU0449EPPCU0449genetic_marker
EPPCU0532EPPCU0532genetic_marker
EPPCU0863EPPCU0863genetic_marker
EPPCU0888EPPCU0888genetic_marker
EPPCU0961EPPCU0961genetic_marker
EPPCU1090EPPCU1090genetic_marker
EPPCU1098EPPCU1098genetic_marker
EPPCU1106EPPCU1106genetic_marker
EPPCU1169EPPCU1169genetic_marker
EPPCU1198EPPCU1198genetic_marker
EPPCU1363EPPCU1363genetic_marker
EPPCU1589EPPCU1589genetic_marker
EPPCU1775EPPCU1775genetic_marker
EPPCU1794EPPCU1794genetic_marker
EPPCU1902EPPCU1902genetic_marker
EPPCU1945EPPCU1945genetic_marker
EPPCU1966EPPCU1966genetic_marker
EPPCU2000EPPCU2000genetic_marker

Pages

Featuremaps
This publication contains information about 2 maps:
Map Name
Prunus Bin Map
Prunus-TE-F2
Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PubMedPMID:16118196