Genetic mapping of the pear scab resistance gene Vnk of Japanese pear cultivar Kinchaku

Publication Overview
TitleGenetic mapping of the pear scab resistance gene Vnk of Japanese pear cultivar Kinchaku
AuthorsTerakami S, Shoda M, Adachi Y, Gonai T, Kasumi M, Sawamura Y, Iketani H, Kotobuki K, Patocchi A, Gessler C, Hayashi T, Yamamoto T
TypeComparative Study
Media TitleTheoretical and Applied Genetics
Volume113
Issue4
Year2006
Page(s)743-752
CitationTerakami S, Shoda M, Adachi Y, Gonai T, Kasumi M, Sawamura Y, Iketani H, Kotobuki K, Patocchi A, Gessler C, Hayashi T, Yamamoto T. Genetic mapping of the pear scab resistance gene Vnk of Japanese pear cultivar Kinchaku. Theoretical and Applied Genetics. 2006 Aug; 113(4):743-752.

Abstract

Pear scab (caused by Venturia nashicola) is one of the most harmful diseases of pears, especially Japanese and Chinese pear species. The molecular identification and early selection of resistant plants could greatly improve pear breeding. We have identified the position of the scab resistance gene, designated Vnk in an indigenous Japanese pear cultivar Kinchaku, within the pear genome by using simple sequence repeat (SSR) markers derived from pear and apple. The position of Vnk was identified in the central region of linkage group 1 of Kinchaku. Several amplified fragment length polymorphism (AFLP) markers linked to Vnk were obtained by bulked segregant analysis. Among them, the AFLP marker closest to Vnk was converted into a sequence tagged site (STS) marker. Four random amplified polymorphic DNA (RAPD) markers previously found to be loosely associated with Vnk (Iketani et al. 2001) were successfully converted into STS markers. Six markers (one SSR Hi02c07 and five STSs converted from AFLP and RAPD) showed tight linkages to Vnk, being mapped with distances ranging from 2.4 to 12.4 cM. The SSR CH-Vf2, which was isolated from a BAC clone of the contig containing the apple scab gene Vf, was mapped at the bottom of linkage group 1 in Kinchaku, suggesting that the Vnk and Vf loci are located in different genomic regions of the same homologous linkage group.

Features
This publication contains information about 17 features:
Feature NameUniquenameType
Hi02c07Hi02c07genetic_marker
PS12A02-1PS12A02-1genetic_marker
AC/CAC-118AC/CAC-118genetic_marker
GT/CAG-205GT/CAG-205genetic_marker
NH013aNH013agenetic_marker
TT/CTT-170TT/CTT-170genetic_marker
CT/CAC-77CT/CAC-77genetic_marker
TA/CTG-83TA/CTG-83genetic_marker
STS-OPW2STS-OPW2genetic_marker
GG/CAC-123GG/CAC-123genetic_marker
STS-CT/CTASTS-CT/CTAgenetic_marker
STS-OPO9STS-OPO9genetic_marker
STS-OPAW13STS-OPAW13genetic_marker
CH-Vf2CH-Vf2genetic_marker
STS-OPAQ11STS-OPAQ11genetic_marker
AG04AG04genetic_marker
resistance to Venturia nashicolapear scab disease resistance-Vnkheritable_phenotypic_marker
Featuremaps
This publication contains information about 2 maps:
Map Name
Pear-Kin1a-F1
Pear-Kin1b-F1