QTL and candidate gene mapping for polyphenolic composition in apple fruit

Publication Overview
TitleQTL and candidate gene mapping for polyphenolic composition in apple fruit
AuthorsChagné D, Krieger C, Rassam M, Sullivan M, Fraser J, André C, Pindo M, Troggio M, Gardiner SE, Henry RA, Allan AC, McGhie TK, Laing WA
TypeJournal Article
Journal NamePlant Biology
CitationQTL and candidate gene mapping for polyphenolic composition in apple fruit. BMC Plant Biology 2012, 12:12


Background The polyphenolic products of the phenylpropanoid pathway, including proanthocyanidins, anthocyanins and flavonols, possess antioxidant properties that may provide health benefits. To investigate the genetic architecture of control of their biosynthesis in apple fruit, various polyphenolic compounds were quantified in progeny from a 'Royal Gala' × 'Braeburn' apple population segregating for antioxidant content, using ultra high performance liquid chromatography of extracts derived from fruit cortex and skin. Results Construction of genetic maps for 'Royal Gala' and 'Braeburn' enabled detection of 79 quantitative trait loci (QTL) for content of 17 fruit polyphenolic compounds. Seven QTL clusters were stable across two years of harvest and included QTLs for content of flavanols, flavonols, anthocyanins and hydroxycinnamic acids. Alignment of the parental genetic maps with the apple whole genome sequence in silico enabled screening for co-segregation with the QTLs of a range of candidate genes coding for enzymes in the polyphenolic biosynthetic pathway. This co-location was confirmed by genetic mapping of markers derived from the gene sequences. Leucoanthocyanidin reductase (LAR1) co-located with a QTL cluster for the fruit flavanols catechin, epicatechin, procyanidin dimer and five unknown procyanidin oligomers identified near the top of linkage group (LG) 16, while hydroxy cinnamate/quinate transferase (HCT/HQT) co-located with a QTL for chlorogenic acid concentration mapping near the bottom of LG 17. Conclusion We conclude that LAR1 and HCT/HQT are likely to influence the concentration of these compounds in apple fruit and provide useful allele-specific markers for marker assisted selection of trees bearing fruit with healthy attributes.
This publication contains information about 69 features:
Feature NameUniquenameType
chlorogenic acid contentqCLGA.RGxBr.2008-ch13(BB)QTL
chlorogenic acid contentqCLGA.RGxBr.2008-ch17(BB)QTL
chlorogenic acid contentqCLGA.RGxBr.2008-ch17(RG)QTL
chlorogenic acid contentqCLGA.RGxBr.2008-ch17(RG).1QTL
chlorogenic acid contentqCLGA.RGxBr.2010-ch17(RG).1QTL
chlorogenic acid contentqCLGA.RGxBr.2010-ch17(RG)QTL
p-coumaroyl quinic acid contentqCQA.RGxBr.2008-ch1(RG)QTL
p-coumaroyl quinic acid contentqCQA.RGxBr.2010-ch1(RG)QTL
p-coumaroyl quinic acid contentqCQA.RGxBr.2008-ch6(BB)QTL
p-coumaroyl quinic acid contentqCQA.RGxBr.2008-ch14(BB)QTL
p-coumaroyl quinic acid contentqCQA.RGxBr.2008-ch14(BB).1QTL
p-coumaroyl quinic acid contentqCQA.RGxBr.2008-ch14(RG)QTL
p-coumaroyl quinic acid contentqCQA.RGxBr.2008-ch14(RG).1QTL
p-coumaroyl quinic acid contentqCQA.RGxBr.2010-ch14(BB)QTL
p-coumaroyl quinic acid contentqCQA.RGxBr.2010-ch14(RG)QTL
p-coumaroyl quinic acid contentqCQA.RGxBr.2008-ch15(BB)QTL
p-coumaroyl quinic acid contentqCQA.RGxBr.2008-ch9(BB)QTL
cyanidin 3-O-arabinoside contentqC3ARA.RGxBr.2008-ch9(RG)QTL
cyanidin 3-O-arabinoside contentqC3ARA.RGxBr.2008-ch9(BB)QTL
cyanidin 3-O-arabinoside contentqC3ARA.RGxBr.2008-ch9(RG).1QTL
cyanidin 3-O-arabinoside contentqC3ARA.RGxBr.2010-ch9(BB)QTL
cyanidin 3-O-arabinoside contentqC3ARA.RGxBr.2010-ch9(RG)QTL
catechin contentqCAT.RGxBr.2008-ch16(BB)QTL
catechin contentqCAT.RGxBr.2008-ch16(RG)QTL
catechin contentqCAT.RGxBr.2010-ch16(BB)QTL


This publication contains information about 2 maps:
Map Name
Apple-RGxBr-Royal Gala-F1