Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region
Publication Overview
Abstract Sharka disease, caused by the Plum pox virus (PPV), is one of
the main limiting factors for stone fruit crops worldwide. Only
a few resistance sources have been found in apricot (Prunus
armeniaca L.), and most studies have located a major PPV
resistance locus (PPVres) on linkage group 1 (LG1). However,
the mapping accuracy was not sufficiently reliable and PPVres
was predicted within a low confidence interval. In this study,
we have constructed two high-density simple sequence repeat
(SSR) improved maps with 0.70 and 0.68 markers/cm, corresponding
to LG1 of ‘Lito’ and ‘Goldrich’ PPV-resistant cultivars,
respectively. Using these maps, and excluding genotype–
phenotype incongruent individuals, a new binary trait locus
(BTL) analysis for PPV resistance was performed, narrowing
down the PPVres support intervals to 7.3 and 5.9 cm in ‘Lito’
and ‘Goldrich’, respectively. Subsequently, 71 overlapping oligonucleotides
(overgo) probes were hybridized against an
apricot bacterial artificial chromosome (BAC) library, identifying
870 single BACs from which 340 were anchored onto a map
region of approximately 30–40 cm encompassing PPVres.
Partial BAC contigs assigned to the two allelic haplotypes
(resistant/susceptible) of the PPVres locus were built by highinformation
content fingerprinting (HICF). In addition, a total of
300 BAC-derived sequences were obtained, and 257 showed
significant homology with the peach genome scaffold_1 corresponding
to LG1. According to the peach syntenic genome
sequence, PPVres was predicted within a region of 2.16 Mb in
which a few candidate resistance genes were identified
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