Construction of a dense genetic linkage map for apple rootstocks using SSRs developed from Malus ESTs and Pyrus genomic sequences

Publication Overview
TitleConstruction of a dense genetic linkage map for apple rootstocks using SSRs developed from Malus ESTs and Pyrus genomic sequences
AuthorsCelton J-M, Tustin DS, Chagne D, Gardiner SE
TypeJournal Article
Journal NameTree Genetics and Genomes
Volume5
Issue1
Year2009
Page(s)93-107
CitationCelton J-M, Tustin DS, Chagne D, Gardiner SE. Construction of a dense genetic linkage map for apple rootstocks using SSRs developed from Malus ESTs and Pyrus genomic sequences. Tree Genetics and Genomes. 2009; 5(1):93-107.

Abstract

Marker-assisted selection (MAS) offers quick and reliable prediction of the phenotypes of seedlings in large populations and thus opens new approaches for selection to breeders of apple (Malus x domestica Borkh.). The development of framework maps enables the discovery of genetic markers linked to desired traits. Although genetic maps have been reported for apple scion cultivars, none has previously been constructed for apple rootstocks. We report the construction of framework genetic maps in a cross between ‘M.9’ (‘Malling 9’) and ‘R.5’ (‘Robusta 5’) apple rootstocks. The maps comprise 224 simple sequence repeat (SSR) markers, 18 sequence-characterised amplified regions, 14 single nucleotide polymorphisms and 42 random amplified polymorphic DNAs. A new set of 47 polymorphic SSRs was developed from apple EST sequences and used for construction of this rootstock map. All 17 linkage groups have been identified and aligned to existing apple genetic maps. The maps span 1,175.7 cM (‘M.9’) and 1,086.7 cM (‘R.5’). To improve the efficiency of mapping markers to this framework map, we developed a bin mapping set. Applications of these new genetic maps include the elucidation of the genetic basis of the dwarfing effect of the apple rootstock ‘M.9’ and the analysis of disease and insect resistance traits such as fire blight (Erwinia amylovora), apple scab (Venturia inaequalis) and woolly apple aphid (Eriosoma lanigerum). Markers for traits mapped in this population will be of direct use to apple breeders for MAS and for identification of causative genes by map-based cloning.
Features
This publication contains information about 257 features:
Feature NameUniquenameType
Hi24f04Hi24f04genetic_marker
Hi23d11Hi23d11genetic_marker
Hi22g06Hi22g06genetic_marker
Hi22f06Hi22f06genetic_marker
Hi08f05Hi08f05genetic_marker
Hi08e04Hi08e04genetic_marker
Hi07d12Hi07d12genetic_marker
Hi05g12Hi05g12genetic_marker
Hi05b09Hi05b09genetic_marker
Hi04g05Hi04g05genetic_marker
Hi03e04Hi03e04genetic_marker
Hi03a03Hi03a03genetic_marker
Hi02g06Hi02g06genetic_marker
Hi02f12Hi02f12genetic_marker
Hi02c07Hi02c07genetic_marker
Hi01d06Hi01d06genetic_marker
CH05h05CH05h05genetic_marker
CH05g07CH05g07genetic_marker
CH05e06CH05e06genetic_marker
CH05d11CH05d11genetic_marker
CH05d04CH05d04genetic_marker
CH05c07CH05c07genetic_marker
CH05c06CH05c06genetic_marker
CH05c04CH05c04genetic_marker
CH05a04CH05a04genetic_marker

Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
Apple-M9xR5-F1-2009