Genetic Analysis of Strawberry Fruit Aroma and Identification of O-Methyltransferase FaOMT as the Locus Controlling Natural Variation in Mesifurane Content

Publication Overview
TitleGenetic Analysis of Strawberry Fruit Aroma and Identification of O-Methyltransferase FaOMT as the Locus Controlling Natural Variation in Mesifurane Content
AuthorsZorrilla-Fontanesi Y, Rambla J, Cabeza A, Medina JJ, Sánchez-Sevilla JF, Valpuesta V, Botella MA, Granell A, Amaya I
TypeJournal Article
Journal NamePlant Physiology
Volume159
Issue2
Year2012
Page(s)851-870
CitationZorrilla-Fontanesi Y, Rambla J, Cabeza A, Medina JJ, Sánchez-Sevilla JF, Valpuesta V, Botella MA, Granell A, Amaya I. Genetic Analysis of Strawberry Fruit Aroma and Identification of O-Methyltransferase FaOMT as the Locus Controlling Natural Variation in Mesifurane Content. Plant Physiology. 2012; 159(2):851-870.

Abstract

Improvement of strawberry (Fragaria x ananassa) fruit flavor is an important goal in breeding programs. To investigate genetic factors controlling this complex trait, a strawberry mapping population derived from genotype ‘1392’, selected for its superior flavor, and ‘232’ was profiled for volatile compounds over 4 years by headspace solid phase microextraction coupled to gas chromatography and mass spectrometry. More than 300 volatile compounds were detected, of which 87 were identified by comparison of mass spectrum and retention time to those of pure standards. Parental line ‘1392’ displayed higher volatile levels than ‘232’, and these and many other compounds with similar levels in both parents segregated in the progeny. Cluster analysis grouped the volatiles into distinct chemically related families and revealed a complex metabolic network underlying volatile production in strawberry fruit. Quantitative trait loci (QTL) detection was carried out over 3 years based on a double pseudo-testcross strategy. Seventy QTLs covering 48 different volatiles were detected, with several of them being stable over time and mapped as major QTLs. Loci controlling γ-decalactone and mesifurane content were mapped as qualitative traits. Using a candidate gene approach we have assigned genes that are likely responsible for several of the QTLs. As a proof of concept we show that one homoeolog of the O-methyltransferase gene (FaOMT) is the locus responsible for the natural variation of mesifurane content. Sequence analysis identified 30 bp in the promoter of this FaOMT homoeolog containing putative binding sites for basic/helix-loop-helix, MYB, and BZIP transcription factors. This polymorphism fully cosegregates with both the presence of mesifurane and the high expression of FaOMT during ripening.
Features
This publication contains information about 207 features:
Feature NameUniquenameType
ethyl acetate contentqEAC.232x1392-ch3M.6.2009QTL
1-penten-3-one contentq1PN3ON.232x1392-ch3M.2QTL
2-pentenal contentq2PAL.232x1392-ch3M.2QTL
butyl acetate contentqBAC.232x1392-ch6F.1.2007QTL
butyl acetate contentqBAC.232x1392-ch6M.1.2007QTL
butyl acetate contentqBAC.232x1392-ch6F.1.2008QTL
butyl acetate contentqBAC.232x1392-ch6M.1.2008QTL
butyl acetate contentqBAC.232x1392-ch6F.1.2009QTL
butyl acetate contentqBAC.232x1392-ch6M.1.2009QTL
methyl pentanoate contentqMPNAT.232x1392-ch1F.1.2008QTL
methyl pentanoate contentqMPNAT.232x1392-ch1M.1b.2008QTL
methyl pentanoate contentqMPNAT.232x1392-ch1F.2.2008QTL
methyl pentanoate contentqMPNAT.232x1392-ch1M.2.2008QTL
methyl pentanoate contentqMPNAT.232x1392-ch1F.2.2009QTL
methyl pentanoate contentqMPNAT.232x1392-ch6F.1.2008QTL
methyl pentanoate contentqMPNAT.232x1392-ch6M.1.2008QTL
3-methylbutyl acetate contentq3MBAC.232x1392-ch3F.2.2007QTL
3-methylbutyl acetate contentq3MBAC.232x1392-ch3M.2.2007QTL
3-methylbutyl acetate contentq3MBAC.232x1392-ch3F.2.2008QTL
3-methylbutyl acetate contentq3MBAC.232x1392-ch3M.2.2008QTL
3-methylbutyl acetate contentq3MBAC.232x1392-ch3F.2.2009QTL
3-methylbutyl acetate contentq3MBAC.232x1392-ch3M.2.2009QTL
3-methylbutyl acetate contentq3MBAC.232x1392-ch3F.6.2008QTL
3-methylbutyl acetate contentq3MBAC.232x1392-ch3M.6.2008QTL
3-methylbutyl acetate contentq3MBAC.232x1392-ch1F.2.2009QTL

Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Strawberry fruit aroma traits-Amaya-2012
Featuremaps
This publication contains information about 2 maps:
Map Name
Strawberry-232x1392-F1
Strawberry-232x1392-F1-2012