A Multidisciplinary Approach Providing New Insight into Fruit Flesh Browning Physiology in Apple (Malus x domestica Borkh.)

Publication Overview
TitleA Multidisciplinary Approach Providing New Insight into Fruit Flesh Browning Physiology in Apple (Malus x domestica Borkh.)
AuthorsDi Guardo M, Tadiello A, Farneti B, Lorenz G, Masuero D, Vrhovsek U, Costa G, Velasco R, Costa F
TypeJournal Article
Journal NamePloS one
Volume8
Issue10
Year2013
Page(s)e78004
CitationDi Guardo M, Tadiello A, Farneti B, Lorenz G, Masuero D, Vrhovsek U, Costa G, Velasco R, Costa F. A Multidisciplinary Approach Providing New Insight into Fruit Flesh Browning Physiology in Apple (Malus x domestica Borkh.). PloS one. 2013; 8(10):e78004.

Abstract

In terms of the quality of minimally processed fruit, flesh browning is fundamentally important in the development of an aesthetically unpleasant appearance, with consequent off-flavours. The development of browning depends on the enzymatic action of the polyphenol oxidase (PPO). In the 'Golden Delicious' apple genome ten PPO genes were initially identified and located on three main chromosomes (2, 5 and 10). Of these genes, one element in particular, here called Md-PPO, located on chromosome 10, was further investigated and genetically mapped in two apple progenies ('Fuji x Pink Lady' and 'Golden Delicious x Braeburn'). Both linkage maps, made up of 481 and 608 markers respectively, were then employed to find QTL regions associated with fruit flesh browning, allowing the detection of 25 QTLs related to several browning parameters. These were distributed over six linkage groups with LOD values spanning from 3.08 to 4.99 and showed a rate of phenotypic variance from 26.1 to 38.6%. Anchoring of these intervals to the apple genome led to the identification of several genes involved in polyphenol synthesis and cell wall metabolism. Finally, the expression profile of two specific candidate genes, up and downstream of the polyphenolic pathway, namely phenylalanine ammonia lyase (PAL) and polyphenol oxidase (PPO), provided insight into flesh browning physiology. Md-PPO was further analyzed and two haplotypes were characterised and associated with fruit flesh browning in apple.

Projects
This publication contains information about 1 projects:
Project NameDescription
Apple-Flesh_browning-Guardo-2013
Features
This publication contains information about 85 features:
Feature NameUniquenameType
KF561112KF561112.1region
KF561111KF561111.1region
PPO_1PPO_1genetic_marker
PPO_2PPO_2genetic_marker
PPO_3PPO_3genetic_marker
PPO_SSR_ch2PPO_SSR_ch2genetic_marker
PPO_SSR_ch5aPPO_SSR_ch5agenetic_marker
PPO_SSR_ch5bPPO_SSR_ch5bgenetic_marker
PPO_SSR_ch5cPPO_SSR_ch5cgenetic_marker
PPO_SSR_ch5dPPO_SSR_ch5dgenetic_marker
PPO_SSR_ch5ePPO_SSR_ch5egenetic_marker
PPO_SSR_ch10aPPO_SSR_ch10agenetic_marker
PPO_SSR_ch10bPPO_SSR_ch10bgenetic_marker
PPO_SSR_ch10cPPO_SSR_ch10cgenetic_marker
PPO_SSR_ch10dPPO_SSR_ch10dgenetic_marker
Md-PALMd-PALgenetic_marker
Md-PPOMd-PPOgenetic_marker
Md-ACTMd-ACTgenetic_marker
CONS40CONS40genetic_marker
CONS14CONS14genetic_marker
CONS43CONS43genetic_marker
CH05eCH05egenetic_marker
CONS5CONS5genetic_marker
CONS93CONS93genetic_marker
CONS67CONS67genetic_marker

Pages

Featuremaps
This publication contains information about 2 maps:
Map Name
Apple-FjxPL-F1-2013
Apple-GDxBr-F1-2013
Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
BraeburnBraeburncultivar
FujiFujicultivar
Pink LadyPink Ladycultivar
Golden DeliciousGolden Deliciouscultivar
Fuji_x_Pink LadyFuji_x_Pink Ladypopulation
GoldenDelicious_x_BraeburnGoldenDelicious_x_Braeburnpopulation
Cross References
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DatabaseAccession
PMID: PubMedPMID:24205065