Putative resistance gene markers associated with quantitative trait loci for fire blight resistance in Malus ‘Robusta 5’ accessions

Publication Overview
TitlePutative resistance gene markers associated with quantitative trait loci for fire blight resistance in Malus ‘Robusta 5’ accessions
AuthorsGardiner SE, Norelli JL, de Silva N, Fazio G, Peil A, Malnoy M, Horner M, Bowatte D, Carlisle C, Wiedow C, Wan Y, Bassett CL, Baldo AM, Celton JM, Richter K, Aldwinckle HS, Bus VG.
TypeJournal Article
Journal NameBMC Genetics
Volume13
Year2012
Page(s)25
CitationGardiner SE, Norelli JL, Silva N, Fazio G, Peil A, Malnoy M, Horner M, Bowatte D, Carlisle C, Wiedow C, Wan Y, Bassett CL, Baldo AM, Celton J-M, Richter K, Aldwinckle HS, Bus VGM. Putative resistance gene markers associated with quantitative trait loci for fire blight resistance in Malus ‘Robusta 5’ accessions. BMC Genetics. 2012; 13:25

Abstract

BACKGROUND: Breeding of fire blight resistant scions and rootstocks is a goal of several international apple breeding programs, as options are limited for management of this destructive disease caused by the bacterial pathogen Erwinia amylovora. A broad, large-effect quantitative trait locus (QTL) for fire blight resistance has been reported on linkage group 3 of Malus 'Robusta 5'. In this study we identified markers derived from putative fire blight resistance genes associated with the QTL by integrating further genetic mapping studies with bioinformatics analysis of transcript profiling data and genome sequence databases. RESULTS: When several defined E.amylovora strains were used to inoculate three progenies from international breeding programs, all with 'Robusta 5' as a common parent, two distinct QTLs were detected on linkage group 3, where only one had previously been mapped. In the New Zealand 'Malling 9' X 'Robusta 5' population inoculated with E. amylovora ICMP11176, the proximal QTL co-located with SNP markers derived from a leucine-rich repeat, receptor-like protein (MxdRLP1) and a closely linked class 3 peroxidase gene. While the QTL detected in the German 'Idared' X 'Robusta 5' population inoculated with E. amylovora strains Ea222_JKI or ICMP11176 was approximately 6 cM distal to this, directly below a SNP marker derived from a heat shock 90 family protein gene (HSP90). In the US 'Otawa3' X 'Robusta5' population inoculated with E. amylovora strains Ea273 or E2002a, the position of the LOD score peak on linkage group 3 was dependent upon the pathogen strains used for inoculation. One of the five MxdRLP1 alleles identified in fire blight resistant and susceptible cultivars was genetically associated with resistance and used to develop a high resolution melting PCR marker. A resistance QTL detected on linkage group 7 of the US population co-located with another HSP90 gene-family member and a WRKY transcription factor previously associated with fire blight resistance. However, this QTL was not observed in the New Zealand or German populations. CONCLUSIONS: The results suggest that the upper region of 'Robusta 5' linkage group 3 contains multiple genes contributing to fire blight resistance and that their contributions to resistance can vary depending upon pathogen virulence and other factors. Mapping markers derived from putative fire blight resistance genes has proved a useful aid in defining these QTLs and developing markers for marker-assisted breeding of fire blight resistance.
Features
This publication contains information about 12 features:
Feature NameUniquenameType
2mo32mo3genetic_marker
5msu35msu3genetic_marker
ARGH31ARGH31genetic_marker
E40M47-75E40M47-75genetic_marker
NZsnEB151679NZsnEB151679genetic_marker
NZsnEH034548NZsnEH034548genetic_marker
resistance to Erwinia amylovoraqREA.M9R5-ch3-R5QTL
resistance to Erwinia amylovoraqREA.IR5-ch3-R5QTL
resistance to Erwinia amylovoraqREA.O3R5-ch3.1-R5QTL
resistance to Erwinia amylovoraqREA.O3R5-ch3.2-R5QTL
resistance to Erwinia amylovoraqREA.O3R5-ch7.1-R5QTL
resistance to Erwinia amylovoraqREA.O3R5-ch7.2-R5QTL
Projects
This publication contains information about 1 projects:
Project NameDescription
Apple fire blight resistance-Gardiner-2012
Featuremaps
This publication contains information about 4 maps:
Map Name
Apple-M9xR5-F1-2012
Apple-IxR5-F1-2012
Apple-O3xR5-US/Ea273-F1
Apple-O3xR5-US/E2002a-F1