Characterization of microsatellite markers in peach [Prunus persica (L.) Batsch]

Publication Overview
TitleCharacterization of microsatellite markers in peach [Prunus persica (L.) Batsch]
AuthorsB. Sosinski, M. Gannavarapu, L. D. Hager, L. E. Beck, G.J. King, C. D. Ryder, S. Rajapakse, W. V. Baird, R. E.
TypeJournal Article
Journal NameTheoretical and Applied Genetics
Volume101
Issue3
Year2000
Page(s)421-428
CitationB. Sosinski, M. Gannavarapu, L. D. Hager, L. E. Beck, G.J. King, C. D. Ryder, S. Rajapakse, W. V. Baird, R. E. Ballard, A. G. Abbott. Characterization of microsatellite markers in peach [Prunus persica (L.) Batsch] Theoretical and Applied Genetics 2000 101 (3):421-428.

Abstract

Microsatellites have emerged as an important system of molecular markers. We evaluated the potential of microsatellites for use in genetic studies of peach [Prunus persica (L.) Batsch]. Microsatellite loci in peach were identified by screening a pUC8 genomic library, a λZAPII leaf cDNA library, as well as through database searches. Primer sequences for the microsatellite loci were tested from the related Rosaceae species apple (Malus×domestica) and sour cherry (Prunus cerasus L.). The genomic library was screened for CT, CA and AGG repeats, while the cDNA library was screened for (CT)n- and (CA)n-containing clones. Estimates of microsatellite frequencies were determined from the genomic library screening, and indicate that CT repeats occur every 100 kb, CA repeats every 420 kb, and AGG repeats every 700 kb in the peach genome. Microsatellite- containing clones were sequenced, and specific PCR primers were designed to amplify the microsatellite- containing regions from genomic DNA. The level of microsatellite polymorphism was evaluated among 28 scion peach cultivars which displayed one to four alleles per primer pair. Five microsatellites were found to segregate in intraspecific peach-mapping crosses. In addition, these microsatellite markers were tested for their utility in cross-species amplification for use in comparative mapping both within the Rosaceae, and with the un- related species Arabidopsis thaliana L.
Features
This publication contains information about 5 features:
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pchcms4pchcms4genetic_marker
pchgms1pchgms1genetic_marker
pchgms3pchgms3genetic_marker