BGT23b, BGT23b (genetic_marker) Pyrus sp.

Marker Overview
NameBGT23b
Genbank IDN/A
TypeSSR
SpeciesPyrus sp.
Primer 1BGT23b.forward: CAC GAC GTT GTA AAA CGA CCAC ATT CAA AGA TTA AGA T
Primer 2BGT23b.reverse: ACT CAG CCT TTT TTT CCC AC
PolymorphismP_ BGT23b
Publication[view all]
CommentGenomic DNA was extracted from leaf tissue using Qiagen DNeasy 96 plant kits (QIAGEN GmbH, Hilden Germany). Sixteen SSR primers were selected for amplification based on previously published studies (Table 2; Liebhard et al. 2002; Yamamoto et al. 2002). The M13 primer, 5’ CACGACGTTGTAAAACGA 3’, with fluorescent dye label (6FAM, or VIC, or NED) was covalently bound to the 5’ end for detection on the ABI 3730 was synthetized from Applied Biosystems (Carlsbad, CA). The two unlabeled primers consisted of a specific SSR-targeting forward primer with a 5’ M13 tail and a specific SSR-targeting reverse primer was synthetized from Sangon Biotech (Shanghai, China). Amplification was carried out in two PCR cycles. The first PCR amplification was performed in a 10μl solution of 20 ng genomic DNA, 1×PCR buffer, 0.25 mM dNTP, 0.6 unit Taq DNA polymerase, 2 mM MgCl2, 0.24μM reverse primer, 0.24 μM forward primer with M13 tail. The first PCR amplification was performed under the following conditions: 94℃ (5 min), 35 cycles at 94℃(30 s)/annealing temperature (An.T) (30 s)/72℃ (45 s), and a final extension at 72℃ for 10 min. using the optimal annealing temperature for each locus (Table 2). The second amplification reaction was performed in a 12.1μl solution of 10μl PCR products of the first PCR circle, 0.3μM fluorescent labeled M13 primer, 0.1× supplied PCR buffer, 0.4 unit of Taq DNA polymerase. The conditions of the second PCR amplification were as follows: 94℃ (5 min), 16 cycles at 94℃ (30 s)/53℃(45 s)/72℃ (45 s), and a final extension at 72℃for10 min. The PCR products were cleaned with ethanol, and then denaturated at 94℃ for 5 min. Finally the fluorescent labeled PCR products at each locus were separated using an ABI 3730 DNA Analyzer (Applied Biosystems, Carlsbad, CA).
Relationships

This genetic_marker is adjacent to the following primer feature(s):

Feature NameUnique NameSpeciesType
forwardBGT23b.forwardPyrus sp.primer
reverseBGT23b.reversePyrus sp.primer


The following marker_locus feature(s) are an instance of this genetic_marker:

Feature NameUnique NameSpeciesType
BGT23bBGT23bPyrus sp.marker_locus
BGT23bBGT23b-28.2Pyrus sp.marker_locus
BGT23bBGT23b-86.22Pyrus sp.marker_locus


Map Positions
#Map NameLinkage GroupBinPositionLocusMapViewer
1Pear-BH-F1Ba2N/A32BGT23bView
2Pear-BD-F1-2014-geneticLG2N/A78.9BGT23bView
3Pear-Bartlett-F1-2007Ba2N/A29.2BGT23bView
4Pear-La_France-F1-2007La2N/A36.3BGT23bView
5Pear-Ba-F1-2013Ba2N/A28.2BGT23bView
6Pear-integrated_consensus_map-IPCG-2017LG2N/A86.22BGT23bView