ddRAD-seq derived genome-wide SNPs, high density linkage map and QTLs for fruit quality traits in strawberry (Fragaria x ananassa).

Publication Overview
TitleddRAD-seq derived genome-wide SNPs, high density linkage map and QTLs for fruit quality traits in strawberry (Fragaria x ananassa).
AuthorsNatarajan S, Hossain MR, Kim HT, Denison MIJ, Ferdous MJ, Jung HJ, Park JI, Nou IS
TypeJournal Article
Journal Name3 Biotech
Volume10
Issue8
Year2020
Page(s)353
CitationNatarajan S, Hossain MR, Kim HT, Denison MIJ, Ferdous MJ, Jung HJ, Park JI, Nou IS. ddRAD-seq derived genome-wide SNPs, high density linkage map and QTLs for fruit quality traits in strawberry (Fragaria x ananassa).. 3 Biotech. 2020 Aug; 10(8):353.

Abstract

Understanding the genetic determinants are essential for improving the fruit quality traits of strawberry. In this study, we focused on mapping the loci for fruit-length (FL), -diameter (FD), -weight (FW) and -soluble solid content (SSC) using the genome-wide single nucleotide polymorphisms (SNPs) identified via ddRAD-sequencing of the F1 population raised from Maehyang (♀) X Festival (♂). A total of 12,698 high quality SNPs were identified of which 1554 SNPs that showed significant Mendelian segregation (p < 0.05) were mapped to 53 linkage groups (LG) spanning a total of 2937.93 cM with an average marker density of 2.14 cM/locus. Six QTLs for FL and four QTLs for each of FD, FW and SSC were identified that explained 24-35%, 21-42%, 24-54% and 23-50% of overall phenotypic variations, respectively. The genes that lie within these QTL regions were extracted and discussed thoroughly. In addition, a high resolution melting marker (MF154) were designed based on the SNP A1723G of the UDP-glucose 4-epimerase GEPI48-like gene FAN_iscf00021287. The marker detected the high vs low sugar containing F1 plants and commercial cultivars with 81.39% and 86.95% detection accuracy, respectively. These SNPs, linkage map, QTLs and candidate genes will be helpful in understanding and improving the fruit quality traits of strawberry.

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Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Strawberry-Fruit_quality-Natarajan-2020
Featuremaps
This publication contains information about 1 maps:
Map Name
Strawberry-Maehyang_x_Festival-F1
Stocks
This publication contains information about 2 stocks:
Stock NameUniquenameType
MaehyangMaehyangaccession
Maehyang_x_Festival-F1Maehyang_x_Festival-F1population