A genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica

Publication Overview
TitleA genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica
AuthorsBushakra JM, Bryant DW, Dossett M, Vining KJ, VanBuren R, Gilmore BS, Lee J, Mockler TC, Finn CE, Bassil NV
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Volume128
Year2015
Page(s)1631-1646
CitationBushakra JM, Bryant DW, Dossett M, Vining KJ, VanBuren R, Gilmore BS, Lee J, Mockler TC, Finn CE, Bassil NV. A genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica. TAG. Theoretical and applied genetics. (2015) 128:1631-1646

Abstract

KEY MESSAGE
We have constructed a densely populated, saturated genetic linkage map of black raspberry and successfully placed a locus for aphid resistance. Black raspberry (Rubus occidentalis L.) is a high-value crop in the Pacific Northwest of North America with an international marketplace. Few genetic resources are readily available and little improvement has been achieved through breeding efforts to address production challenges involved in growing this crop. Contributing to its lack of improvement is low genetic diversity in elite cultivars and an untapped reservoir of genetic diversity from wild germplasm. In the Pacific Northwest, where most production is centered, the current standard commercial cultivar is highly susceptible to the aphid Amphorophora agathonica Hottes, which is a vector for the Raspberry mosaic virus complex. Infection with the virus complex leads to a rapid decline in plant health resulting in field replacement after only 3-4 growing seasons. Sources of aphid resistance have been identified in wild germplasm and are used to develop mapping populations to study the inheritance of these valuable traits. We have constructed a genetic linkage map using single-nucleotide polymorphism and transferable (primarily simple sequence repeat) markers for F1 population ORUS 4305 consisting of 115 progeny that segregate for aphid resistance. Our linkage map of seven linkage groups representing the seven haploid chromosomes of black raspberry consists of 274 markers on the maternal map and 292 markers on the paternal map including a morphological locus for aphid resistance. This is the first linkage map of black raspberry and will aid in developing markers for marker-assisted breeding, comparative mapping with other Rubus species, and enhancing the black raspberry genome assembly.

Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
JewelJewelcultivar
NC84-10-3NC84-10-3cultivar
ORUS3778-1ORUS3778-1cultivar
ORUS3021-2ORUS3021-2cultivar
ORUS4153-1ORUS4153-1cultivar
ORUS3021-2_x_ORUS4153-1ORUS3021-2_x_ORUS4153-1population
Features
This publication contains information about 442 features:
Feature NameUniquenameType
Ag4_AphidRAg4_AphidRgenetic_marker
Rh_MEa13bG01Rh_MEa13bG01genetic_marker
Rh_MEa13cF08Rh_MEa13cF08genetic_marker
Rh_MEa2cA01Rh_MEa2cA01genetic_marker
Rh_MEa6bG05Rh_MEa6bG05genetic_marker
Rh_MEa7aB01Rh_MEa7aB01genetic_marker
Ri1407Ri1407genetic_marker
RiCHEa1B16RiCHEa1B16genetic_marker
Ro_CBE2P01bRo_CBE2P01bgenetic_marker
Ro_CBEa0001L24Ro_CBEa0001L24genetic_marker
Ro_CBEa0002P01bRo_CBEa0002P01bgenetic_marker
Ro_CBEa0003K17Ro_CBEa0003K17genetic_marker
Ro_CBEa0004G23Ro_CBEa0004G23genetic_marker
Ro_CBEa0009J05Ro_CBEa0009J05genetic_marker
Ro_CBEa10N20Ro_CBEa10N20genetic_marker
Ro_CBEa11M11Ro_CBEa11M11genetic_marker
Ro_CBEa1L24Ro_CBEa1L24genetic_marker
Ro_CBEa3K17Ro_CBEa3K17genetic_marker
Ro_CBEa4G23Ro_CBEa4G23genetic_marker
Ro_CBEa9J05Ro_CBEa9J05genetic_marker
Ro9324S289Ro9324S289genetic_marker
Rub110aRub110agenetic_marker
RUB116aRUB116agenetic_marker
Rub126bRub126bgenetic_marker
Rub16aRub16agenetic_marker

Pages

Featuremaps
This publication contains information about 3 maps:
Map Name
Black_Raspberry-ORUS3021-2-F1
Black_Raspberry-ORUS4153-1-F1
Black_Raspberry-O3xO4-F1
Properties
Additional details for this publication include:
Property NameValue
eISSN1432-2242
ISSN1432-2242
Journal AbbreviationTheor. Appl. Genet.
LanguageEnglish
Language AbbrENG
Publication Date2015 Jun 3
Publication ModelPrint-Electronic
Publication TypeJournal Article
Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PubMedPMID:26037086