High-density mapping suggests cytoplasmic male sterility with two restorer genes in almond × peach progenies

Publication Overview
TitleHigh-density mapping suggests cytoplasmic male sterility with two restorer genes in almond × peach progenies
AuthorsDonoso JM, Eduardo I, Picañol R, Batlle I, Howad W, Aranzana MJ, Arús P.
TypeJournal Article
Journal NameHorticulture Research
Volume2
Year2015
CitationDonoso JM, Eduardo I, Picañol R, Batlle I, Howad W, Aranzana MJ, Arús P. High-density mapping suggests cytoplasmic male sterility with two restorer genes in almond × peach progenies. Horticulture Research 2015 2: 15016

Abstract

Peach (Prunus persica) and almond (Prunus dulcis) are two sexually compatible species that produce fertile offspring. Almond, a highly polymorphic species, is a potential source of new genes for peach that has a strongly eroded gene pool. Here we describe the genetics of a male sterile phenotype that segregated in two almond (‘Texas’) × peach (‘Earlygold’) progenies: an F2 (T×E) and a backcross one (T1E) to the ‘Earlygold’ parent. High-density maps were developed using a 9k peach SNP chip and 135 simple-sequence repeats. Three highly syntenic and collinear maps were obtained: one for the F2 (T×E) and two for the backcross, T1E (for the hybrid) and E (for ‘Earlygold’). A major reduction of recombination was observed in the interspecific maps (T×E and T1E) compared to the intraspecific parent (E). The E map also had extensive monomorphic genomic regions suggesting the presence of large DNA fragments identical by descent. Our data for the male sterility character were consistent with the existence of cytoplasmic male sterility, where individuals having the almond cytoplasm required the almond allele in at least one of two independent restorer genes, Rf1 and Rf2, to be fertile. The restorer genes were located in a 3.4 Mbp fragment of linkage group 2 (Rf1) and 1.4 Mbp of linkage group 6 (Rf2). Both fragments contained several genes coding for pentatricopeptide proteins, demonstrated to be responsible for restoring fertility in other species. The implications of these results for using almond as a source of novel variability in peach are discussed.
File Attachments
AttachmentSize
File TxE_GDR.xlsx868.74 KB
File T1E_GDR.xlsx2.71 MB
File E_GDR.xlsx816.01 KB
File MS_GDR.xlsx1.47 MB
Features
This publication contains information about 480 features:
Feature NameUniquenameType
SNP_IGA_869278SNP_IGA_869278genetic_marker
SNP_IGA_870119SNP_IGA_870119genetic_marker
SNP_IGA_913799SNP_IGA_913799genetic_marker
SNP_IGA_95340SNP_IGA_95340genetic_marker
SNP_IGA_95950SNP_IGA_95950genetic_marker
SNP_IGA_97126SNP_IGA_97126genetic_marker
RosCOS0544-090RosCOS0544-090genetic_marker
RosCOS1328-366_snp_sweet_cherry_Pp2_22162942RosCOS1328-366_snp_sweet_cherry_Pp2_22162942genetic_marker
RosCOS3786-396_snp_sweet_cherry_Pp5_16429681RosCOS3786-396_snp_sweet_cherry_Pp5_16429681genetic_marker
snp_1_39059187snp_1_39059187genetic_marker
snp_2_22274363snp_2_22274363genetic_marker
SNP_IGA_101065SNP_IGA_101065genetic_marker
SNP_IGA_101331SNP_IGA_101331genetic_marker
SNP_IGA_101815SNP_IGA_101815genetic_marker
SNP_IGA_102819SNP_IGA_102819genetic_marker
SNP_IGA_103507SNP_IGA_103507genetic_marker
SNP_IGA_105122SNP_IGA_105122genetic_marker
SNP_IGA_106000SNP_IGA_106000genetic_marker
SNP_IGA_10635SNP_IGA_10635genetic_marker
SNP_IGA_106646SNP_IGA_106646genetic_marker
SNP_IGA_108298SNP_IGA_108298genetic_marker
SNP_IGA_108299SNP_IGA_108299genetic_marker
SNP_IGA_109223SNP_IGA_109223genetic_marker
SNP_IGA_109619SNP_IGA_109619genetic_marker
SNP_IGA_109897SNP_IGA_109897genetic_marker

Pages

Featuremaps
This publication contains information about 3 maps:
Map Name
Prunus-TE-F2-2015
Prunus-TE-BC1-T1E
Prunus-TE-BC1-E
Properties
Additional details for this publication include:
Property NameValue
URLhttp://www.nature.com/articles/hortres201516