Fine mapping of the Rvi5 (Vm) apple scab resistance locus in the ‘Murray’ apple genotype

Publication Overview
TitleFine mapping of the Rvi5 (Vm) apple scab resistance locus in the ‘Murray’ apple genotype
AuthorsCova V, Bandara NL, Liang W, Tartarini S, Patocchi A, Troggio M, Velasco R, Komjanc M
TypeJournal Article
Volume35
Year2015
Page(s)200
CitationCova V, Bandara NL, Liang W, Tartarini S, Patocchi A, Troggio M, Velasco R, Komjanc M. Fine mapping of the Rvi5 (Vm) apple scab resistance locus in the ‘Murray’ apple genotype. Molecular Breeding. 2015. 35: 200.

Abstract

Apple scab, caused by the fungal pathogen Venturia inaequalis, is the most devastating pathogen in the apple-growing industry. In the last two decades, many studies have been initiated to identify new resistances to apple scab and to introgress them into new cultivars through breeding. The Rvi6 gene from Malus floribunda 821 has been the one most intensively used in breeding programmes worldwide, but the identification of new pathogen strains that are virulent to Rvi6 has increased the need for pyramiding of more than one resistance gene to obtain cultivars with durable resistance. Here, we report on the fine mapping of the Rvi5 apple scab resistance locus using two large segregating populations. A region of about 1 cM at the distal end of LG17 carrying the Rvi5 resistance gene was detailed by developing and mapping 10 molecular markers, including SCAR, SSR and SNP markers. The Rvi5 locus was restricted to a region of 228 kb on the ‘Golden Delicious’ reference genome between the two flanking SSR markers FMACH_Vm4 and FMACH_Vm2. Three co-segregating molecular markers were also developed (SSR FMACH_Vm3, Vm–SCAR1 and Vm-SNP5). All these markers will facilitate the development of marker-assisted selection protocols for this gene using both low-cost methods and high-throughput systems. The findings of this study will thus be useful for further investigation of the Rvi5 resistance locus of ‘Murray’, aimed at candidate gene identification and the physical isolation of the resistance gene.
Features
This publication contains information about 15 features:
Feature NameUniquenameType
CH04f08CH04f08genetic_marker
Hi02f12Hi02f12genetic_marker
OPB12_SCAROPB12_SCARgenetic_marker
CH05d08CH05d08genetic_marker
Hi07h02Hi07h02genetic_marker
FMACH_Vm4FMACH_Vm4genetic_marker
FMACH_Vm3FMACH_Vm3genetic_marker
FMACH_Vm2FMACH_Vm2genetic_marker
FMACH_Vm1FMACH_Vm1genetic_marker
Vm-SNP1Vm-SNP1genetic_marker
Vm-SNP2Vm-SNP2genetic_marker
Vm-SNP3Vm-SNP3genetic_marker
Vm-SNP4Vm-SNP4genetic_marker
Vm-SNP5Vm-SNP5genetic_marker
resistance to Venturia inaequalisScab resistance-Rvi5heritable_phenotypic_marker
Featuremaps
This publication contains information about 1 maps:
Map Name
Apple-GDxM-F1