Narrowing down the single homoeologous FaPFRU locus controlling flowering in cultivated octoploid strawberry using a selective mapping strategy

Publication Overview
TitleNarrowing down the single homoeologous FaPFRU locus controlling flowering in cultivated octoploid strawberry using a selective mapping strategy
AuthorsPerrotte J, Gaston A, Potier A, Petit A, Rothan C, Denoyes B
TypeJournal Article
Journal NamePlant Biotechnology Journal
Volume14
Issue11
Year2016
Page(s)2176-2189
CitationPerrotte J, Gaston A, Potier A, Petit A, Rothan C, Denoyes B . Narrowing down the single homoeologous FaPFRU locus controlling flowering in cultivated octoploid strawberry using a selective mapping strategy. Plant Biotechnology Journal. 2016; 14(11):2176-2189.

Abstract

Extending the period of fruit production is a way to substantially increase crop yield in many fruit or ornamental species. In the cultivated octoploid strawberry (Fragaria × ananassa), the most consumed small fruit worldwide, fruit production season can be extended by selecting the perpetual flowering (PF) cultivars. This trait is of considerable interest to growers and to the food industry. Four homoeologous loci controlling a single trait can be expected in such a complex octoploid species. However, we recently showed that the PF trait is under the control of the single dominant FaPFRU locus (J. Exp. Bot., 2013, 64, 1837), making it potentially amenable to marker-assisted selection (MAS). Here, we report the successful use of a strategy, based on a selective mapping using a reduced sample of individuals, to identify nine markers in close linkage to the FaPFRU allelic variant. Thus, this strategy can be used to fine map the target homoeologous loci in other complex polyploid crop species. Recombinant analysis further enabled us to reduce the locus to a region flanked by two markers, Bx083_206 and Bx215_131, corresponding to a 1.1 Mb region in the diploid F. vesca reference genome. This region comprises 234 genes, including 15 flowering associated genes. Among these, the FLOWERING LOCUS T (FT) is known to be a key activator of flowering. The close association between the PF trait and the FaPFRU flanking markers was validated using an additional segregating population and genetic resources. This study lays the foundation for effective and rapid breeding of PF strawberry cultivars by MAS.
Features
This publication contains information about 135 features:
Feature NameUniquenameType
tcaa317tcaa317genetic_marker
b008161b008161genetic_marker
b008163b008163genetic_marker
ccaa220ccaa220genetic_marker
gtat238gtat238genetic_marker
b008165b008165genetic_marker
ars4277ars4277genetic_marker
b026121b026121genetic_marker
tcaa360tcaa360genetic_marker
b032154b032154genetic_marker
catc270catc270genetic_marker
tggx318tggx318genetic_marker
tgat165tgat165genetic_marker
gtgx380gtgx380genetic_marker
gtta242gtta242genetic_marker
gaac250gaac250genetic_marker
tgtg215tgtg215genetic_marker
tgtg237tgtg237genetic_marker
u008109u008109genetic_marker
tgtc328tgtc328genetic_marker
tgag400tgag400genetic_marker
gctg308gctg308genetic_marker
cctc242cctc242genetic_marker
r55e178r55e178genetic_marker
i102272i102272genetic_marker

Pages

Projects
This publication contains information about 1 projects:
Project NameDescription
Strawberry-perpetual_flowering-Denoyes-2016
Featuremaps
This publication contains information about 3 maps:
Map Name
Strawberry-CxCF-F1-2016f
Strawberry-CxCF-F1-2016m
Strawberry-CxCF-F1-Capitola-2016
Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
CF1116CF1116accession
Capitola_x_CF1116-F1-2016Capitola_x_CF1116-F1-2016population
CapitolaCapitolaaccession