The Peach v2.0 release: high-resolution linkage mapping and deep resequencing improve chromosome-scale assembly and contiguity

Publication Overview
TitleThe Peach v2.0 release: high-resolution linkage mapping and deep resequencing improve chromosome-scale assembly and contiguity
AuthorsVerde I, Jenkins J, Dondini L, Micali S, Pagliarani G, Vendramin E, Paris R, Aramini V, Gazza L, Rossini L, Bassi D, Troggio M, Shu S, Grimwood J, Tartarini S, Dettori MT, Schmutz J
TypeJournal Article
Journal NameBMC genomics
Volume18
Issue1
Year2017
Page(s)225
CitationVerde I, Jenkins J, Dondini L, Micali S, Pagliarani G, Vendramin E, Paris R, Aramini V, Gazza L, Rossini L, Bassi D, Troggio M, Shu S, Grimwood J, Tartarini S, Dettori MT, Schmutz J. The Peach v2.0 release: high-resolution linkage mapping and deep resequencing improve chromosome-scale assembly and contiguity. BMC genomics. 2017 Mar 11; 18(1):225.

Abstract

BACKGROUND
The availability of the peach genome sequence has fostered relevant research in peach and related Prunus species enabling the identification of genes underlying important horticultural traits as well as the development of advanced tools for genetic and genomic analyses. The first release of the peach genome (Peach v1.0) represented a high-quality WGS (Whole Genome Shotgun) chromosome-scale assembly with high contiguity (contig L50 214.2 kb), large portions of mapped sequences (96%) and high base accuracy (99.96%). The aim of this work was to improve the quality of the first assembly by increasing the portion of mapped and oriented sequences, correcting misassemblies and improving the contiguity and base accuracy using high-throughput linkage mapping and deep resequencing approaches.

RESULTS
Four linkage maps with 3,576 molecular markers were used to improve the portion of mapped and oriented sequences (from 96.0% and 85.6% of Peach v1.0 to 99.2% and 98.2% of v2.0, respectively) and enabled a more detailed identification of discernible misassemblies (10.4 Mb in total). The deep resequencing approach fixed 859 homozygous SNPs (Single Nucleotide Polymorphisms) and 1347 homozygous indels. Moreover, the assembled NGS contigs enabled the closing of 212 gaps with an improvement in the contig L50 of 19.2%.

CONCLUSIONS
The improved high quality peach genome assembly (Peach v2.0) represents a valuable tool for the analysis of the genetic diversity, domestication, and as a vehicle for genetic improvement of peach and related Prunus species. Moreover, the important phylogenetic position of peach and the absence of recent whole genome duplication (WGD) events make peach a pivotal species for comparative genomics studies aiming at elucidating plant speciation and diversification processes.

Features
This publication contains information about 533 features:
Feature NameUniquenameType
DCAS11_305881DCAS11_305881genetic_marker
DCAS11_663201DCAS11_663201genetic_marker
DCAS12_456548DCAS12_456548genetic_marker
DCAS12_644162DCAS12_644162genetic_marker
DCAS12_668994DCAS12_668994genetic_marker
DCAS13_560511DCAS13_560511genetic_marker
DCAS14_258268DCAS14_258268genetic_marker
DCAS14_526857DCAS14_526857genetic_marker
DCAS15_12748DCAS15_12748genetic_marker
DCAS15_214189DCAS15_214189genetic_marker
DCAS16_301284DCAS16_301284genetic_marker
DCAS17_318485DCAS17_318485genetic_marker
DCAS18_41535DCAS18_41535genetic_marker
DCAS2_1005038DCAS2_1005038genetic_marker
DCAS2_10181662DCAS2_10181662genetic_marker
DCAS2_10707212DCAS2_10707212genetic_marker
DCAS2_11440211DCAS2_11440211genetic_marker
DCAS2_12914582DCAS2_12914582genetic_marker
DCAS2_13506543DCAS2_13506543genetic_marker
DCAS2_14225514DCAS2_14225514genetic_marker
DCAS2_14910313DCAS2_14910313genetic_marker
DCAS2_15813909DCAS2_15813909genetic_marker
DCAS2_16518447DCAS2_16518447genetic_marker
DCAS2_2201732DCAS2_2201732genetic_marker
DCAS2_3135931DCAS2_3135931genetic_marker

Pages

Featuremaps
This publication contains information about 6 maps:
Map Name
Prunus-TxE-peachV2.0
Peach-CA-BC1-2017
Peach-PF-BC1-2017
Peach-PF-F1-2017
Peach-PF-Recurrent-2017
Prunus-MDxSD-2017
Stocks
This publication contains information about 4 stocks:
Stock NameUniquenameType
Texas_x_Earligold-F2Texas_x_Earligold-F2population
IF7310828_x_Ferganensis-BC1IF7310828_x_Ferganensis-BC1population
Contender_x _Ambra-F2Contender_x _Ambra-F2population
Maria Dolce_x_SD81-F1Maria Dolce_x_SD81-F1population