Microsatellite markers spanning the apple (Malus x domestica Borkh.) genome

Publication Overview
TitleMicrosatellite markers spanning the apple (Malus x domestica Borkh.) genome
AuthorsSilfverberg-Dilworth E, Matasci CL, Van de Weg WE, Van Kaauwen MPW, Walser M, Kodde LP, Soglio V, Gianfranceschi L, Durel CE, Costa F, Yamamoto T, Koller B, Gessler C Patocchi A
TypeJournal Article
Journal NameTree Genetics and Genomes
Volume2
Issue4
Year2006
Page(s)202-224
CitationSilfverberg-Dilworth E, Matasci CL, Van de Weg WE, Van Kaauwen MPW, Walser M, Kodde LP, Soglio V, Gianfranceschi L, Durel CE, Costa F, Yamamoto T, Koller B, Gessler C Patocchi A. Microsatellite markers spanning the apple (Malus x domestica Borkh.) genome. Tree Genetics and Genomes. 2006; 2(4):202-224.

Abstract

A new set of 148 apple microsatellite markers has been developed and mapped on the apple reference linkage map Fiesta x Discovery. One-hundred and seventeen markers were developed from genomic libraries enriched with the repeats GA, GT, AAG, AAC and ATC; 31 were developed from EST sequences. Markers derived from sequences containing dinucleotide repeats were generally more polymorphic than sequences containing trinucleotide repeats. Additional eight SSRs from published apple, pear, and Sorbus torminalis SSRs, whose position on the apple genome was unknown, have also been mapped. The transferability of SSRs across Maloideae species resulted in being efficient with 41% of the markers successfully transferred. For all 156 SSRs, the primer sequences, repeat type, map position, and quality of the amplification products are reported. Also presented are allele sizes, ranges, and number of SSRs found in a set of nine cultivars. All this information and those of the previous CH-SSR series can be searched at the apple SSR database (http://www. hidras.unimi.it) to which updates and comments can be added. A large number of apple ESTs containing SSR repeats are available and should be used for the development of new apple SSRs. The apple SSR database is also meant to become an international platform for coordinating this effort. The increased coverage of the apple genome with SSRs allowed the selection of a set of 86 reliable, highly polymorphic, and overall the apple genome well-scattered SSRs. These SSRs cover about 85% of the genome with an average distance of one marker per 15 cM.
Features
This publication contains information about 998 features:
Feature NameUniquenameType
CN444794-SSRCN444794-SSRgenetic_marker
CN444636-SSRCN444636-SSRgenetic_marker
CN444542-SSRCN444542-SSRgenetic_marker
CH05h05CH05h05genetic_marker
CH05g11CH05g11genetic_marker
CH05g08CH05g08genetic_marker
CH05g03CH05g03genetic_marker
CH05f06CH05f06genetic_marker
CH05f04CH05f04genetic_marker
CH05e06CH05e06genetic_marker
CH05e05CH05e05genetic_marker
CH05d11CH05d11genetic_marker
CH05d04CH05d04genetic_marker
CH05d03CH05d03genetic_marker
CH05d02CH05d02genetic_marker
CH05c07CH05c07genetic_marker
CH05c06CH05c06genetic_marker
CH05c04CH05c04genetic_marker
CH05a09CH05a09genetic_marker
CH05a05CH05a05genetic_marker
CH05a04CH05a04genetic_marker
CH04h02CH04h02genetic_marker
CH04g10CH04g10genetic_marker
CH04g07CH04g07genetic_marker
CH04g04CH04g04genetic_marker

Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
Apple-FD-F1-2006