A genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica

Publication Overview
TitleA genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica
AuthorsBushakra JM, Bryant DW, Dossett M, Vining KJ, VanBuren R, Gilmore BS, Lee J, Mockler TC, Finn CE, Bassil NV
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Volume128
Year2015
Page(s)1631-1646
CitationBushakra JM, Bryant DW, Dossett M, Vining KJ, VanBuren R, Gilmore BS, Lee J, Mockler TC, Finn CE, Bassil NV. A genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica. TAG. Theoretical and applied genetics. (2015) 128:1631-1646

Abstract

KEY MESSAGE
We have constructed a densely populated, saturated genetic linkage map of black raspberry and successfully placed a locus for aphid resistance. Black raspberry (Rubus occidentalis L.) is a high-value crop in the Pacific Northwest of North America with an international marketplace. Few genetic resources are readily available and little improvement has been achieved through breeding efforts to address production challenges involved in growing this crop. Contributing to its lack of improvement is low genetic diversity in elite cultivars and an untapped reservoir of genetic diversity from wild germplasm. In the Pacific Northwest, where most production is centered, the current standard commercial cultivar is highly susceptible to the aphid Amphorophora agathonica Hottes, which is a vector for the Raspberry mosaic virus complex. Infection with the virus complex leads to a rapid decline in plant health resulting in field replacement after only 3-4 growing seasons. Sources of aphid resistance have been identified in wild germplasm and are used to develop mapping populations to study the inheritance of these valuable traits. We have constructed a genetic linkage map using single-nucleotide polymorphism and transferable (primarily simple sequence repeat) markers for F1 population ORUS 4305 consisting of 115 progeny that segregate for aphid resistance. Our linkage map of seven linkage groups representing the seven haploid chromosomes of black raspberry consists of 274 markers on the maternal map and 292 markers on the paternal map including a morphological locus for aphid resistance. This is the first linkage map of black raspberry and will aid in developing markers for marker-assisted breeding, comparative mapping with other Rubus species, and enhancing the black raspberry genome assembly.

Features
This publication contains information about 442 features:
Feature NameUniquenameType
RO_S671_62748RO_S671_62748genetic_marker
RO_S68_477340RO_S68_477340genetic_marker
RO_S681_58391RO_S681_58391genetic_marker
RO_S684_63660RO_S684_63660genetic_marker
RO_S689_55838RO_S689_55838genetic_marker
RO_S7_295570RO_S7_295570genetic_marker
RO_S7_379506RO_S7_379506genetic_marker
RO_S70_150228RO_S70_150228genetic_marker
RO_S705_264391RO_S705_264391genetic_marker
RO_S705_64391RO_S705_64391genetic_marker
RO_S71_420905RO_S71_420905genetic_marker
RO_S714_73912RO_S714_73912genetic_marker
RO_S721_59214RO_S721_59214genetic_marker
RO_S723_48232RO_S723_48232genetic_marker
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RO_S73_33754RO_S73_33754genetic_marker
RO_S733_27643RO_S733_27643genetic_marker
RO_S75_381030RO_S75_381030genetic_marker
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RO_S76_223569RO_S76_223569genetic_marker
RO_S77_150939RO_S77_150939genetic_marker
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RO_S78_386742RO_S78_386742genetic_marker

Pages

Featuremaps
This publication contains information about 3 maps:
Map Name
Black_Raspberry-ORUS3021-2-F1
Black_Raspberry-ORUS4153-1-F1
Black_Raspberry-O3xO4-F1
Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
JewelJewelaccession
NC84-10-3NC84-10-3accession
ORUS3778-1ORUS3778-1accession
ORUS3021-2ORUS3021-2accession
ORUS4153-1ORUS4153-1accession
ORUS3021-2_x_ORUS4153-1ORUS3021-2_x_ORUS4153-1population