A genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica

Publication Overview
TitleA genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica
AuthorsBushakra JM, Bryant DW, Dossett M, Vining KJ, VanBuren R, Gilmore BS, Lee J, Mockler TC, Finn CE, Bassil NV
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Volume128
Year2015
Page(s)1631-1646
CitationBushakra JM, Bryant DW, Dossett M, Vining KJ, VanBuren R, Gilmore BS, Lee J, Mockler TC, Finn CE, Bassil NV. A genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica. TAG. Theoretical and applied genetics. (2015) 128:1631-1646

Abstract

KEY MESSAGE
We have constructed a densely populated, saturated genetic linkage map of black raspberry and successfully placed a locus for aphid resistance. Black raspberry (Rubus occidentalis L.) is a high-value crop in the Pacific Northwest of North America with an international marketplace. Few genetic resources are readily available and little improvement has been achieved through breeding efforts to address production challenges involved in growing this crop. Contributing to its lack of improvement is low genetic diversity in elite cultivars and an untapped reservoir of genetic diversity from wild germplasm. In the Pacific Northwest, where most production is centered, the current standard commercial cultivar is highly susceptible to the aphid Amphorophora agathonica Hottes, which is a vector for the Raspberry mosaic virus complex. Infection with the virus complex leads to a rapid decline in plant health resulting in field replacement after only 3-4 growing seasons. Sources of aphid resistance have been identified in wild germplasm and are used to develop mapping populations to study the inheritance of these valuable traits. We have constructed a genetic linkage map using single-nucleotide polymorphism and transferable (primarily simple sequence repeat) markers for F1 population ORUS 4305 consisting of 115 progeny that segregate for aphid resistance. Our linkage map of seven linkage groups representing the seven haploid chromosomes of black raspberry consists of 274 markers on the maternal map and 292 markers on the paternal map including a morphological locus for aphid resistance. This is the first linkage map of black raspberry and will aid in developing markers for marker-assisted breeding, comparative mapping with other Rubus species, and enhancing the black raspberry genome assembly.

Features
This publication contains information about 442 features:
Feature NameUniquenameType
RO_S780_47083RO_S780_47083genetic_marker
RO_S780_9754RO_S780_9754genetic_marker
RO_S781_3964RO_S781_3964genetic_marker
RO_S79_351594RO_S79_351594genetic_marker
RO_S798_31088RO_S798_31088genetic_marker
RO_S799_55672RO_S799_55672genetic_marker
RO_S80_151911RO_S80_151911genetic_marker
RO_S80_303146RO_S80_303146genetic_marker
RO_S804_55106RO_S804_55106genetic_marker
RO_S81_168310RO_S81_168310genetic_marker
RO_S818_21981RO_S818_21981genetic_marker
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RO_S8331915RO_S8331915genetic_marker
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RO_S842_37425RO_S842_37425genetic_marker
RO_S85_209336RO_S85_209336genetic_marker
RO_S850_14502RO_S850_14502genetic_marker
RO_S86_272676RO_S86_272676genetic_marker
RO_S865_3480RO_S865_3480genetic_marker
RO_S87_253649RO_S87_253649genetic_marker
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RO_S88_93014RO_S88_93014genetic_marker
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RO_S90_260135RO_S90_260135genetic_marker
RO_S921_19750RO_S921_19750genetic_marker

Pages

Featuremaps
This publication contains information about 3 maps:
Map Name
Black_Raspberry-ORUS3021-2-F1
Black_Raspberry-ORUS4153-1-F1
Black_Raspberry-O3xO4-F1
Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
JewelJewelaccession
NC84-10-3NC84-10-3accession
ORUS3778-1ORUS3778-1accession
ORUS3021-2ORUS3021-2accession
ORUS4153-1ORUS4153-1accession
ORUS3021-2_x_ORUS4153-1ORUS3021-2_x_ORUS4153-1population