A genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica

Publication Overview
TitleA genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica
AuthorsBushakra JM, Bryant DW, Dossett M, Vining KJ, VanBuren R, Gilmore BS, Lee J, Mockler TC, Finn CE, Bassil NV
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Volume128
Year2015
Page(s)1631-1646
CitationBushakra JM, Bryant DW, Dossett M, Vining KJ, VanBuren R, Gilmore BS, Lee J, Mockler TC, Finn CE, Bassil NV. A genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag 4 conferring resistance to the aphid Amphorophora agathonica. TAG. Theoretical and applied genetics. (2015) 128:1631-1646

Abstract

KEY MESSAGE
We have constructed a densely populated, saturated genetic linkage map of black raspberry and successfully placed a locus for aphid resistance. Black raspberry (Rubus occidentalis L.) is a high-value crop in the Pacific Northwest of North America with an international marketplace. Few genetic resources are readily available and little improvement has been achieved through breeding efforts to address production challenges involved in growing this crop. Contributing to its lack of improvement is low genetic diversity in elite cultivars and an untapped reservoir of genetic diversity from wild germplasm. In the Pacific Northwest, where most production is centered, the current standard commercial cultivar is highly susceptible to the aphid Amphorophora agathonica Hottes, which is a vector for the Raspberry mosaic virus complex. Infection with the virus complex leads to a rapid decline in plant health resulting in field replacement after only 3-4 growing seasons. Sources of aphid resistance have been identified in wild germplasm and are used to develop mapping populations to study the inheritance of these valuable traits. We have constructed a genetic linkage map using single-nucleotide polymorphism and transferable (primarily simple sequence repeat) markers for F1 population ORUS 4305 consisting of 115 progeny that segregate for aphid resistance. Our linkage map of seven linkage groups representing the seven haploid chromosomes of black raspberry consists of 274 markers on the maternal map and 292 markers on the paternal map including a morphological locus for aphid resistance. This is the first linkage map of black raspberry and will aid in developing markers for marker-assisted breeding, comparative mapping with other Rubus species, and enhancing the black raspberry genome assembly.

Features
This publication contains information about 442 features:
Feature NameUniquenameType
RO_S30_582093RO_S30_582093genetic_marker
RO_S31_314682RO_S31_314682genetic_marker
RO_S314_148478RO_S314_148478genetic_marker
RO_S314_179125RO_S314_179125genetic_marker
RO_S316_186017RO_S316_186017genetic_marker
RO_S318_155707RO_S318_155707genetic_marker
RO_S32_375704RO_S32_375704genetic_marker
RO_S323_22819RO_S323_22819genetic_marker
RO_S325_109080RO_S325_109080genetic_marker
RO_S327_60426RO_S327_60426genetic_marker
RO_S329_82033RO_S329_82033genetic_marker
RO_S33_584984RO_S33_584984genetic_marker
RO_S331_120820RO_S331_120820genetic_marker
RO_S332_25238RO_S332_25238genetic_marker
RO_S335_26380RO_S335_26380genetic_marker
RO_S337_128476RO_S337_128476genetic_marker
RO_S337_42274RO_S337_42274genetic_marker
RO_S337-126476RO_S337-126476genetic_marker
RO_S34_759158RO_S34_759158genetic_marker
RO_S341_24920RO_S341_24920genetic_marker
RO_S344_12492RO_S344_12492genetic_marker
RO_S348_11401RO_S348_11401genetic_marker
RO_S35_190714RO_S35_190714genetic_marker
RO_S353_156014RO_S353_156014genetic_marker
RO_S355_30318RO_S355_30318genetic_marker

Pages

Featuremaps
This publication contains information about 3 maps:
Map Name
Black_Raspberry-ORUS3021-2-F1
Black_Raspberry-ORUS4153-1-F1
Black_Raspberry-O3xO4-F1
Stocks
This publication contains information about 6 stocks:
Stock NameUniquenameType
JewelJewelaccession
NC84-10-3NC84-10-3accession
ORUS3778-1ORUS3778-1accession
ORUS3021-2ORUS3021-2accession
ORUS4153-1ORUS4153-1accession
ORUS3021-2_x_ORUS4153-1ORUS3021-2_x_ORUS4153-1population