Rubus L. subgenus Rubus Watson Genome v1.0 Assembly & Annotation

Summary:
The tetraploid genome assembly for thornless tetraploid blackberry selection BL1 (Rubus L. subgenus Rubus Watson) was generated using the Oxford Nanopore Technology (ONT) and Hi-C scaffolding, comprising 919 Mb placed on 27 pseudochromosomes with an N50 of 35.73 Mb. ONT data was assembled using NECAT/0.0.1. Gene prediction was performed using the BRAKER/v2 pipeline utilizing the transcriptome generated from RNA-Seq of thorny shoots, thornless shoots, leaves, green fruits, and publicly available RNA-Seq datasets for blackberry. RepeatMasker/4.1.1 was used for annotation of repetitive elements. 

Citation: 
Paudel, D., Peng, Z., Parajuli, S., Parrish, S. B., & Deng, Z. (2023). A chromosome-scale and haplotype-resolved genome assembly of tetraploid blackberry (Rubus L. subgenus Rubus Watson). bioRxiv, 2023-04.