Prunus library information

 Library Name  Organism  Cultivar  Development Stage  Tissue Type  Number of ESTs  Note
 LIBEST_015606 Prunus persica fruit mesocarp final maturation steps, post-climateric  Prunus persica    final maturation steps, post-climateric  fruit mesocarp  23  tissue_lib: S4 ; country: Italy
 LIBEST_027714 Pr_av_leaf_bud  Prunus avium    swollen bud  leaf bud  6014  E.coli NEB 10-beta
 LIBEST_016673 Prunus persica fruit mesocarp plus epidermis 80 days after bloom  Prunus persica  Fantasia  80 days after bloom  fruit mesocarp plus epidermis  928  
 LIBEST_012965 Ripe Apricot Fruit Lambda Zap II Library  Prunus armeniaca  Bergeron  Ripe stage    4166  Organ: Fruit; Vector: Lambda Zap II; Site_1: Eco RI; Site_2: XhoI; Oriented library, construction described in Molecular cloning and expression of a cDNA encoding 1-aminocyclopropane-1-carboxylate (ACC) oxidase from apricot fruit (Prunus armeniaca cv. Bergeron) by Mbeguie-Mbeguie D, Chahine H, Gomez RM, Gouble B, Audergon JM, Souty M, Albagnac G, Fils-Lycaon B in Physiol Plant 105:294-303 1999
 LIBEST_021001 Subtraction suppressive hybridization library A Forward  Prunus persica  Canadian Harmony on Tennessee Natural rootstock  current years growth  bark (mostly cambium)  12  Organ: branch; Vector: pCR2.1; Site_1: EcoR1; Site_2: EcoR1; Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturers protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the A Forward library represent sequences downregulated at 5oC in nightbreak.
 LIBEST_026267 Fruits and flowers of Prunus mume cv. Xiyeqing  Prunus mume  Xiyeqing  mixed  Fruits and flowers  4473  Organ: Fruits and flowers; Vector: pDNR-LIB; Fruits and flowers of Prunus mume Xiyeqing at different development stages were collected from the resource nursery of Nanjing Agricultural University, Najing PRC. The materials were collected from 2009.2-2009.5. The flowers included four growing stages: small bud, medium bud, half-unfolding flower and completely unfolding flower, while the fruits were collected at 0.3, 0.6, 0.9, 1.2, 1.8, 2.4, 3.6 cm diameter stages.
 LIBEST_019256 PU3  Prunus persica  O`Henry    Mesocarp  6639  Organ: Fruit; Vector: pDNR-1r; Site_1: XhoI; Site_2: SmaI
 LIBEST_017722 Prunus armeniaca cv. Goldrich  Prunus armeniaca  Goldrich      24  
 LIBEST_020208 YG/PR  Prunus cerasus  Montmorency  Young-green and pre-ripe  Pericarp  1243  Organ: Fruit
 LIBEST_009616 Cherry cDNA Library  Prunus avium        21  
 LIBEST_011149 Peach developing fruit mesocarp  Prunus persica  Loring    Mesocarp  9984  Vector: pBluescript II SK(-); Site_1: EcoRI; Site_2: XhoI; authority=Prunus persica L. Batsh; The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis go to http://www.genome.clemson.edu/projects/peach. To order this clone go to http://www.genome.clemson.edu/orders
 LIBEST_012964 Green Apricot Fruit Lambda Zap II Library  Prunus armeniaca  Bergeron  Green stage    5380  Organ: Fruit; Vector: Lambda Zap II; Site_1: Eco RI; Site_2: XhoI; Oriented library, construction described in Molecular cloning and expression of a cDNA encoding 1-aminocyclopropane-1-carboxylate (ACC) oxidase from apricot fruit (Prunus armeniaca cv. Bergeron) by Mbeguie-Mbeguie D, Chahine H, Gomez RM, Gouble B, Audergon JM, Souty M, Albagnac G, Fils-Lycaon B in Physiol Plant 105:294-303 1999
 LIBEST_016335 S3  Prunus persica    from pre-climacteric to climacteric  fruit mesocarp  2919  
 LIBEST_022200 TDFs from plum cv. Cacanska Najbolja  Prunus domestica  Cacanska Najbolja    leaf  30  Vector: pGEM-T; cDNA-AFLP was performed to identyfy genes differentially expressed in plum upon Plum pox virus (PPV) infection
 LIBEST_022919 Subtraction suppressive hybridization library C Reverse  Prunus persica  Canadian Harmony on Tennessee Natural rootstock  current years growth  bark (mostly cambium)  23  Organ: branch; Vector: pCR2.1; Site_1: EcoR1; Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMCRT system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturers protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the C Reverse library represent sequences downregulated during night break photoperiods at 5oC. Night break simulates a long day (16 h light/8 h dark), but the actual light exposure is equivalent to a short day (8 h light/16 h dark) photoperiod.
 LIBEST_018884 PU1  Prunus persica  O`Henry    Mesocarp  9729  Organ: Fruit; Vector: pDNR-1r; Site_1: XhoI; Site_2: SmaI
 LIBEST_017153 Prunus persica L. leaf tissue, Plum Pox Virus infected  Prunus persica  Baby gold 5    Leaf  2144  Leaf tissue of Prunus persica L. infected by Plum pox virus
 LIBEST_019257 PU4  Prunus persica  O`Henry    Mesocarp  6634  Organ: Fruit; Vector: pDNR-1r; Site_1: XhoI; Site_2: SmaI
 LIBEST_022201 TDFs from plum cv. Cacanska Rodna  Prunus domestica  Cacanska Rodna    leaf  24  Vector: pGEM-T; cDNA-AFLP was performed to identyfy genes differentially expressed in plum upon Plum pox virus (PPV) infection
 LIBEST_027858 Peach fruit after harvest Lib  Prunus persica  Dixiland    mesocarp  126  Organ: fruit
 LIBEST_012963 Half-Ripe Apricot Fruit Lambda Zap II Library  Prunus armeniaca  Bergeron  Half-Ripe stage    5522  Organ: Fruit; Vector: Lambda Zap II; Site_1: Eco RI; Site_2: XhoI; Oriented library, construction described in Molecular cloning and expression of a cDNA encoding 1-aminocyclopropane-1-carboxylate (ACC) oxidase from apricot fruit (Prunus armeniaca cv. Bergeron) by Mbeguie-Mbeguie D, Chahine H, Gomez RM, Gouble B, Audergon JM, Souty M, Albagnac G, Fils-Lycaon B in Physiol Plant 105:294-303 1999
 LIBEST_016334 S4  Prunus persica    post-climacteric  fruit mesocarp  2908  
 LIBEST_009614 Almond Lambda Zap II cDNA Library  Prunus dulcis        64  synonym: Prunus amygdalus
 LIBEST_008685 sour cherry fruit library  Prunus cerasus  Montmorency  8 days or 44 days after pollination    12  Organ: Fruit; Vector: pBluescriptSK; Site_1: 5-EcoRI; Site_2: 3-XhoI; PRA and PRB libraries were constructed with mRNA extracted from fruits harvested 44 days after pollination. YGC and YGD libraries were constructed from mRNA extracted from fruits 8 days after pollination. TAG_LIB=YGD
 LIBEST_010977 almond cDNA library  Prunus dulcis      pistils  1006  Organ: flower; Vector: pZL1; Site_1: Sal I; Site_2: Not I; Total RNAs were isolated from pistils using Trizol reagent (Invitrogen, USA). Then, polyA+ mRNA was isolated using oligo(dT) cellulose as described. cDNA was synthesized using a lambda-zipLox cDNA synthesis kit(CAT No.19643-014, Invitrogen, USA). The phage library was converted through mass excision to a plasmid library in the vector pZL1. The plasmid library was plated on 15-cm LB agar plates with 100ug/mL ampicillin. Individual clones were picked at random and propagated. The 5ends of the cDNA clones were sequenced on ABI Prism377 DNA sequencer.
 LIBEST_010509 Apricot cDNA library  Prunus armeniaca  Stark Early Orange  Shoot bursting, post-dormancy    11  Organ: leaf; Vector: pGEM-T
 LIBEST_024792 RD  Prunus persica    adult  flower bud  233  Vector: pGEM-T Easy; Enriched in dormancy released bud
 LIBEST_018175 Cherry differentially expressed  Prunus avium x P. cerasus x P. canescens  Bing/Gisela5 or Bing/Gisela6    Xylem, Bark  89  Organ: Stem; ve samples were excised from the cDNA-AFLP gel and amplified by PCR. The PCR product was cloned in the pGEM-T Easy vector and sequenced with the M13 forward primer.
 LIBEST_024303 Subtraction library (endodormant buds minus ecodormant buds)  Prunus mume  Nanko    lateral buds  6  country: Japan ; lat_lon: 34 N 135 E
 LIBEST_015035 S3II  Prunus persica  Redhaven  S3II, 95 days after full bloom  mesocarp  1267  
 LIBEST_024793 ZS  Prunus persica    adult  flower bud  62  Vector: pGEM-T Easy; Enriched in Zincal 5 dormancy released bud
 LIBEST_024302 Prunus mume lateral buds  Prunus mume  Nanko    lateral buds  1  country: Japan ; lat_lon: 34 N 135 E
 LIBEST_022917 Subtraction suppressive hybridization library B Reverse  Prunus persica  Canadian Harmony on Tennessee Natural rootstock  current years growth  bark (mostly cambium)  42  Organ: branch; Vector: pCR2.1; Site_1: EcoR1; Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturers protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the B Reverse library represent sequences downregulated during long day (16 h light/8 h dark) photoperiods at 25oC.
 LIBEST_010486 Prunus persica fruit  Prunus persica      fruit  4  
 LIBEST_016672 Prunus persica fruit mesocarp plus epidermis 30 days after bloom  Prunus persica  Fantasia  30 days after bloom  fruit mesocarp plus epidermis  1647  
 LIBEST_024791 DR  Prunus persica    adult  flower bud  96  Vector: pGEM-T Easy; Enriched in dormant bud
 LIBEST_022411 PP1  Prunus persica  Pop-DG  adult trees  mesocarp  720  Organ: fruits; Full-length cDNA library made from a mix of poly-A+ RNA from mesocarp of peach fruits stored at 5 degrees for different times, and showing symptoms of chilling injury.
 LIBEST_022916 Subtraction suppressive hybridization library B Forward  Prunus persica  Canadian Harmony on Tennessee Natural rootstock  current years growth  bark (mostly cambium)  46  Organ: branch; Vector: pCR2.1; Site_1: EcoR1; Site_2: EcoR1; Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturers protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the B Forward library represent sequences upregulated during long day photoperiods (16 h light/8 h dark) at 25oC.
 LIBEST_027181 Suppression Subtractive Hybridization Library of Floral Buds in Prunus persica during Domancy-Releasing  Prunus persica      floral Bud  9  
 LIBEST_016802 S2  Prunus persica    endocarp hardening  fruit mesocarp  156  
 LIBEST_022920 Subtraction suppressive hybridization library E Forward  Prunus persica  Canadian Harmony on Tennessee Natural rootstock  current years growth  bark (mostly cambium)  16  Organ: branch; Vector: pCR2.1; Site_1: EcoR1; Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturers protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the E Forward library represent sequences upregulated at 5oC during exposure to short day (8 h light/16 h dark) photoperiods.
 LIBEST_007302 near-ripe peach fruit cDNA library  Prunus persica  Loring  21-60 Newtons flesh resistance  Whole fruit minus the stone  174  Vector: Lambda vector UniZap XR; Site_1: EcoRI; Site_2: XhoI; Fruit cDNA. The mRNA was isolated from whole fruit from which the stone was excised. The fruit was near ripe, registering from 21 to 60 newtons resistance to a penetrometer. The library was constructed using the lambda UniZap XR by Stratagene.
 LIBEST_015605 Prunus persica fruit mesocarp final maturation steps, pre-climateric  Prunus persica    final maturation steps, pre-climateric  fruit mesocarp  12  tissue_lib: S3 ; country: Italy
 LIBEST_017154 Prunus persica L. leaf tissue, healthy  Prunus persica  Baby gold 5    Leaf  1625  Leaf tissue of Prunus persica L. not infected by Plum pox virus
 LIBEST_018856 Peach shoot  Prunus persica  Loring    Shoot  7085  Vector: pBluescript II SK(-); Site_1: EcoRI; Site_2: XhoI
 LIBEST_025548 Floral organ cDNA library of Mei flower  Prunus mume        92  Vector: lTripIEx2
 LIBEST_022918 Subtraction suppressive hybridization library C Forward  Prunus persica  Canadian Harmony on Tennessee Natural rootstock  current years growth  bark (mostly cambium)  22  Organ: branch; Vector: pCR2.1; Site_1: EcoR1; Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturers protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the C Forward library represent sequences upregulated during night break photoperiods at 5oC. Night break simulates a long day (16 h light/8 h dark) photoperiod, but the actual light exposure is equivalent to a short day (8 h light/16 h dark) exposure.
 LIBEST_022412 PPN  Prunus persica  Pop-DG  adult trees  mesocarp  7142  Organ: fruits; Full-length, normalized cDNA library made from a mix of poly-A+ RNA from mesocarp of peach fruits stored at 5 degress for different times, and showing symptoms of chilling injury.
 LIBEST_009893 Prunus persica green fruit  Prunus persica  Suncrest    green fruit  1  
 LIBEST_018885 PU2  Prunus persica  O`Henry    Mesocarp  9495  Organ: Fruit; Vector: pDNR-1r; Site_1: XhoI; Site_2: SmaI
 LIBEST_015940 Apricot cv. Colomer cDNA library  Prunus armeniaca  Colomer  grafted scion    2  Organ: leaf; Vector: pGEM-T; mRNA extracted from leaf samples originating from apricot grafted scion
 LIBEST_011273 Almond developing seed  Prunus dulcis  Nonpareil    embryo  2794  Vector: pBK-CMV; Site_1: EcoRI; Site_2: XhoI; synonym: Prunus amygdalus
 LIBEST_017210 Peach developing fruit mesocarp Stage S4  Prunus persica  Yumyeong    Mesocarp  1667  Vector: pDONR222
 LIBEST_024005 Differentially expressed cDNAs between wild-type and evergrowing Prunus persica from long-days to short-days transition  Prunus persica    current years growth  apical bud tip  180  Vector: pGEM-T easy; Total RNA was isolated from apical bud tips from peach. After DNase I treatment (Invitrogen, Carlsbad, CA, USA), polyA+ RNA was purified using Dynabeads Oligo(dT)25 (Invitrogen). The cDNA was synthetised and the suppression subtractive hybridization (SSH) between WT and evg samples at LD and one, two, four and eight weeks of SD was performed following the manufacturers protocol (Clontech Laboratories, Palo Alto, CA, USA). PCR-amplified products were cloned into pGEM-T easy vector (Promega, Madison, WI, USA). 5,760 clones from subtracted cDNA libraries were screened by hybridization with forward- and reverse-subtracted radiolabeled cDNA. Selected clones from all time points and directions were sequenced using pGEM-T easy vector primer.
 LIBEST_016803 S1  Prunus persica    post-fertilization  fruit mesocarp  137  
 LIBEST_024301 Subtraction library (endodormant buds minus paradormant buds)  Prunus mume  Nanko    lateral buds  17  country: Japan ; lat_lon: 34 N 135 E
 LIBEST_009615 Peach cDNA Library  Prunus persica        22  
 LIBEST_026655 PLMVd-infected GF-305 peach tree leaves  Prunus persica  GF-305    Leaves  13  Sequence obtained by cDNA-AFLP
 LIBEST_020449 Prunus persica fruit skin mature fruit  Prunus persica  Bolero  mature fruit  fruit skin  4690  country: Italy
 LIBEST_024794 SZ  Prunus persica    adult  flower bud  73  Vector: pGEM-T Easy; Enriched in Springlady dormancy released bud
 LIBEST_021000 Subtraction suppressive hybridization library A Reverse  Prunus persica  Canadian Harmony on Tennessee Natural rootstock  current years growth  bark (mostly cambium)  21  Organ: branch; Vector: pCR2.1; Site_1: EcoR1; Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturers protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the A Reverse library (AR library) represent sequences upregulated at 5oC in nightbreak.
 LIBEST_015585 Prunus persica mesocarp S4 climacteric  Prunus persica  Fantasia  S4 climacteric  mesocarp  1049  
 LIBEST_022921 Subtraction suppressive hybridization library E Reverse  Prunus persica  Canadian Harmony on Tennessee Natural rootstock  current years growth  bark (mostly cambium)  40  Organ: branch; Vector: pCR2.1; Site_1: EcoR1; Total RNA was prepared from peach bark and used as a template for cDNA synthesis using the SuperSMART system (Clontech, Palo Alto, CA). Amplification of cDNAs and suppression subtractive hybridization (SSH) followed the manufacturers protocol for SSH (Clontech). PCR-amplified products resulting from two rounds of hybridization were cloned non-directionally into pCR2.1 (Invitrogen, Carlsbad, CA). Colony PCR using M13 forward and reverse primers was used to identify recombinant plasmids containing inserts >200 bp. Recombinant plasmid DNA was sequenced in both directions using Nested PCR primers 1 and 2R (Clontech) or M13 forward and reverse primers. The sequences obtained from the E Reverse library represent sequences downregulated at 5oC during exposure to short day (8 h light/16 h dark) photoperiods.