ppa025850m, ppa025850m (mRNA) Prunus persica

BLAST of ppa025850m vs. Vitis vinifera
Match: GSVIVT01033872001 (assembled CDS)

HSP 1 Score: 709.523 bits (1830), Expect = 0.000e+0
Identity = 353/569 (62.04%), Postives = 426/569 (74.87%), Query Frame = 1

Query:   40 NLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAE----------------------------CRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            ++ +   ++ MYA+   +  A  +F  M E++ ++WS M+  +   G        FR ++ SG+ PD  TL  ++  C + ++L   R VH     HG+L +      LV  Y+  + +DDA  LFD M  RD V+W+VM+G FA+                            C NA+ESLVLFD M EEGVVPDKVAMVT+V ACAKLGAMH+AR +  YI R +F LDVILGTAMIDM+AKCGC++SAREIFDRM+ KNVI+WSAMIAAYGYHG GRKA+ +F +ML SG+ PN IT VSLLYACSH+GL+EEGLR FSLMW++++VRAD+KHYTC+VDLLGRAGRLDEAL+L+ESM VEKDE LWGA LGACR H ++ LAEKA  SLLELQP+N GHY+LLSNIYA AGRW+DVAK RDLMSQRRLKK+PG TWIEVDNK++QFS GD  HP+S++IY ML+SLG KLEL GYVPDTNFVLHDVDEE+K+ +LYSHSEKLAIAFGLIAT + TPIRI KNLRVCGDCHTF K VSA+T RVIIVRD NRFHHF+EG CSCGDYW
Sbjct:  293 DVILGTAMIDMYAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFRMMLSSGMLPDKITLASLLYACINCRNLTQVRQVHAQASVHGMLQNLIVANKLVHFYSYYRALDDAYGLFDGMCVRDSVSWSVMVGGFAKVGDYMNCFGTFRELIRCGARPDNYTLPFCGNANESLVLFDKMREEGVVPDKVAMVTVVFACAKLGAMHKARTIDDYIQRKKFQLDVILGTAMIDMHAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFPMMLRSGILPNKITLVSLLYACSHAGLVEEGLRFFSLMWEDYSVRADVKHYTCVVDLLGRAGRLDEALKLIESMTVEKDEGLWGAFLGACRTHKDVVLAEKAATSLLELQPQNPGHYILLSNIYANAGRWEDVAKTRDLMSQRRLKKIPGWTWIEVDNKSHQFSVGDTTHPRSKEIYEMLKSLGNKLELVGYVPDTNFVLHDVDEELKIGILYSHSEKLAIAFGLIATPEHTPIRIIKNLRVCGDCHTFCKLVSAITGRVIIVRDANRFHHFKEGACSCGDYW 861          

HSP 2 Score: 391.349 bits (1004), Expect = 5.667e-109
Identity = 206/362 (56.91%), Postives = 255/362 (70.44%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTC---MVDLLGRAGRLDEALELVESMAVEKDERLWGALLG 1077
            +VHAQ  V+GML+N+ VANKL+Y Y+  R L DAY LF GM  RDSV+WSVMVGGF  VGD+ N F TFRELIR G  PD YTLPFVIR CRD+K+L+M                     ALVDMY KC+ ++DAR LFDKMQ RDLVTWTVMIG +AEC  A+ESLVLF+ M EEGVVPDKVAMVT+V ACAKLGAMH+AR++  YI R +F LDVILGTAMIDMYAKCGC++SAREIFDRM+ KNVI+WSAMIAAYGYHG GRKA+ +F +ML+SG+ P+ IT  SLLYAC +   + +  ++ +    + +V   +++      +V        LD+A  L + M V +D   W  ++G
Sbjct:   99 QVHAQASVHGMLENIVVANKLIYFYSYYRALDDAYGLFDGMCVRDSVSWSVMVGGFAKVGDYINCFGTFRELIRCGARPDNYTLPFVIRACRDLKNLQM---------------------ALVDMYVKCREIEDARFLFDKMQERDLVTWTVMIGGYAECGKANESLVLFEKMREEGVVPDKVAMVTVVFACAKLGAMHKARIIDDYIQRKKFQLDVILGTAMIDMYAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFRMMLSSGMLPDKITLASLLYACINCRNLTQVRQVHA----QASVHGMLQNLIVANKLVHFYSYYRALDDAYGLFDGMCV-RDSVSWSVMVG 434          

HSP 3 Score: 191.045 bits (484), Expect = 1.124e-48
Identity = 106/292 (36.30%), Postives = 161/292 (55.14%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPF----------------------------VIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSL--DVILGTAMIDMYAKCGCIDSAREIFDRMQV-KNVITWSAMIAAYGYH 783
            +VHAQ  V+GMLQNL VANKL++ Y+  R L DAY LF GM  RDSV+WSVMVGGF  VGD+ N F TFRELIR G  PD YTLPF                            V+  C  +  +   R + D + +     D     A++DM+AKC  V+ AR++FD+M+ +++++W+ MI A+       ++L LF  M+  G++P+K+ +V+++ AC+  G +    L  + +    +S+  DV   T ++D+  + G +D A ++ + M V K+   W A + A   H
Sbjct:  381 QVHAQASVHGMLQNLIVANKLVHFYSYYRALDDAYGLFDGMCVRDSVSWSVMVGGFAKVGDYMNCFGTFRELIRCGARPDNYTLPFCGNANESLVLFDKMREEGVVPDKVAMVTVVFACAKLGAMHKARTIDDYIQRKKFQLDVILGTAMIDMHAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFPMMLRSGILPNKITLVSLLYACSHAGLVEEG-LRFFSLMWEDYSVRADVKHYTCVVDLLGRAGRLDEALKLIESMTVEKDEGLWGAFLGACRTH 671          

HSP 4 Score: 176.792 bits (447), Expect = 2.194e-44
Identity = 102/386 (26.42%), Postives = 190/386 (49.22%), Query Frame = 1

Query:   34 LQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYG----------------------------YHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVR----ADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            L+NL +A  L+ MY + R + DA  LF  M ERD VTW+VM+GG+   G        F ++   GV PD   +  V+  C  +  +   R++ D + +     D     A++DMYAKC  V+ AR++FD+M+ +++++W+ MI A+       ++L LF  M+  G++PDK+ + +++ AC     + + R +H     +    ++I+   ++  Y+    +D A  +FD M V++ ++WS M+  +                             + G+  +++ +F  M   GV P+ +  V++++AC+  G + +   I     D++  R     D+   T M+D+  + G ++ A E+ + M  EK+   W A++ A   HG
Sbjct:  192 LKNLQMA--LVDMYVKCREIEDARFLFDKMQERDLVTWTVMIGGYAECGKANESLVLFEKMREEGVVPDKVAMVTVVFACAKLGAMHKARIIDDYIQRKKFQLDVILGTAMIDMYAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFRMMLSSGMLPDKITLASLLYACINCRNLTQVRQVHAQASVHGMLQNLIVANKLVHFYSYYRALDDAYGLFDGMCVRDSVSWSVMVGGFAKVGDYMNCFGTFRELIRCGARPDNYTLPFCGNANESLVLFDKMREEGVVPDKVAMVTVVFACAKLGAMHKARTI-----DDYIQRKKFQLDVILGTAMIDMHAKCGCVESAREIFDRME-EKNVISWSAMIAAYGYHG 569          

BLAST of ppa025850m vs. Vitis vinifera
Match: GSVIVT01012159001 (assembled CDS)

HSP 1 Score: 493.812 bits (1270), Expect = 8.107e-140
Identity = 248/545 (45.50%), Postives = 351/545 (64.40%), Query Frame = 1

Query:   55 NKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADN-FACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIE-EGVVPDKVAMVTIVNACA-KLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSG-----VSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDE-RLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            N L+ MYA+   + D+ ALF    +RD V+W+ M+  F     F+   A FR ++  GV  D  T+  V+  C  ++ L +G+ +H  VL++  L +N F  +ALVDMY  C+ V+  R++FD +  R +  W  MI  +A     +++L+LF  MI+  G++P+   M +++ AC   L A+ + + +H Y  RN  + D+ +G+A++DMYAKCGC++ +R +F+ M  KNVITW+ +I A G HG G +A+ +F  M+          PN +TF+++  ACSHSGLI EGL +F  M  +  V     HY C+VDLLGRAG+L+EA ELV +M  E D+   W +LLGACRIH N++L E A  +LL L+P  A HYVLLSNIY+ AG W    ++R  M Q  +KK PG +WIE  ++ ++F  GD  HPQSE+++  LE+L +K+   GYVPDT+ VLH+VDE+ K  +L  HSEKLAIAFG++ T  GT IR+ KNLRVC DCH  TKF+S + +R IIVRDV RFHHF+EGTCSCGDYW
Sbjct:  152 NALMAMYAKLGRVDDSKALFESFVDRDMVSWNTMISSFSQSDRFSEALAFFRLMVLEGVELDGVTIASVLPACSHLERLDVGKEIHAYVLRNNDLIENSFVGSALVDMYCNCRQVESGRRVFDHILGRRIELWNAMISGYARNGLDEKALILFIEMIKVAGLLPNTTTMASVMPACVHSLAAIAKGKEIHAYAIRNMLASDITVGSALVDMYAKCGCLNLSRRVFNEMPNKNVITWNVLIMACGMHGKGEEALELFKNMVAEAGRGGEAKPNEVTFITVFAACSHSGLISEGLNLFYRMKHDHGVEPTSDHYACVVDLLGRAGQLEEAYELVNTMPAEFDKVGAWSSLLGACRIHQNVELGEVAAKNLLHLEPNVASHYVLLSNIYSSAGLWNKAMEVRKNMRQMGVKKEPGCSWIEFRDEVHKFMAGDVSHPQSEQLHGFLETLSEKMRKEGYVPDTSCVLHNVDEDEKENLLCGHSEKLAIAFGILNTPPGTTIRVAKNLRVCNDCHAATKFISKIMEREIIVRDVRRFHHFKEGTCSCGDYW 696          

HSP 2 Score: 165.622 bits (418), Expect = 5.057e-41
Identity = 110/385 (28.57%), Postives = 186/385 (48.31%), Query Frame = 1

Query:  130 RDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACA---------------------ALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLD-VILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLN-SGVSPNVITFVSLLYACSHS-GLIEEGLRIFSLMWDEFAVR----ADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGR 1200
            R + +W   +       DF    +T+ E+  SG  PD +  P V++    ++DLK G  +H   +K G  + +   A                     AL+ MYAK   VDD++ LF+   +RD+V+W  MI +F++     E+L  F  M+ EGV  D V + +++ AC+ L  +   + +H Y+ RN   ++   +G+A++DMY  C  ++S R +FD +  + +  W+AMI+ Y  +G   KA+ +F  M+  +G+ PN  T  S++ AC HS   I +G  I +     +A+R    +DI   + +VD+  + G L+ +  +   M   K+   W  L+ AC +HG     E+A+               L  N+ A AGR
Sbjct:   55 RSTASWVDALRSRTRSNDFREAISTYIEMTVSGARPDNFAFPAVLKAVSGLQDLKTGEQIHAAAVKFGYGSSSVTVANTLVNMYGKCGGIGDKTFTNNALMAMYAKLGRVDDSKALFESFVDRDMVSWNTMISSFSQSDRFSEALAFFRLMVLEGVELDGVTIASVLPACSHLERLDVGKEIHAYVLRNNDLIENSFVGSALVDMYCNCRQVESGRRVFDHILGRRIELWNAMISGYARNGLDEKALILFIEMIKVAGLLPNTTTMASVMPACVHSLAAIAKGKEIHA-----YAIRNMLASDITVGSALVDMYAKCGCLNLSRRVFNEMP-NKNVITWNVLIMACGMHGK---GEEALE--------------LFKNMVAEAGR 416          

HSP 3 Score: 105.531 bits (262), Expect = 6.211e-23
Identity = 80/318 (25.16%), Postives = 157/318 (49.37%), Query Frame = 1

Query:    1 EVHAQVVVNG-MLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIR-SGVTPDIYTLPFVIRVC-RDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEG-----VVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILG--TAMIDMYAKCGCIDSAREIFDRM--QVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVIT-FVSLLYACSHSGLIEEGLRI 915
            E+HA V+ N  +++N  V + L+ MY   R +     +F  +  R    W+ M+ G+   G        F E+I+ +G+ P+  T+  V+  C   +  +  G+ +H   +++ L +D    +ALVDMYAKC  ++ +R++F++M N++++TW V+I A       +E+L LF  M+ E        P++V  +T+  AC+  G +     L +Y  ++   ++        ++D+  + G ++ A E+ + M  +   V  WS+++ A   H +          +L+  + PNV + +V L    S +GL  + + +
Sbjct:  235 EIHAYVLRNNDLIENSFVGSALVDMYCNCRQVESGRRVFDHILGRRIELWNAMISGYARNGLDEKALILFIEMIKVAGLLPNTTTMASVMPACVHSLAAIAKGKEIHAYAIRNMLASDITVGSALVDMYAKCGCLNLSRRVFNEMPNKNVITWNVLIMACGMHGKGEEALELFKNMVAEAGRGGEAKPNEVTFITVFAACSHSGLISEGLNL-FYRMKHDHGVEPTSDHYACVVDLLGRAGQLEEAYELVNTMPAEFDKVGAWSSLLGACRIHQNVELGEVAAKNLLH--LEPNVASHYVLLSNIYSSAGLWNKAMEV 549          

HSP 4 Score: 65.855 bits (159), Expect = 5.454e-11
Identity = 52/258 (20.16%), Postives = 112/258 (43.41%), Query Frame = 1

Query:  430 NRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQF-SLDVILGTAMIDMYAKCGCI--------------------DSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLEL 1140
            +R   +W   + +     +  E++  +  M   G  PD  A   ++ A + L  +     +H    +  + S  V +   +++MY KCG I                    D ++ +F+    +++++W+ MI+++       +A+A F LM+  GV  + +T  S+L ACSH   ++ G  I + +     +  +    + +VD+     +++    + + +   + E LW A++     +G   L EKA+   +E+
Sbjct:   54 SRSTASWVDALRSRTRSNDFREAISTYIEMTVSGARPDNFAFPAVLKAVSGLQDLKTGEQIHAAAVKFGYGSSSVTVANTLVNMYGKCGGIGDKTFTNNALMAMYAKLGRVDDSKALFESFVDRDMVSWNTMISSFSQSDRFSEALAFFRLMVLEGVELDGVTIASVLPACSHLERLDVGKEIHAYVLRNNDLIENSFVGSALVDMYCNCRQVESGRRVFDHILGRRIE-LWNAMISGYARNG---LDEKALILFIEM 307          

BLAST of ppa025850m vs. Vitis vinifera
Match: GSVIVT01008721001 (assembled CDS)

HSP 1 Score: 484.182 bits (1245), Expect = 6.424e-137
Identity = 239/556 (42.99%), Postives = 357/556 (64.21%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVN---VGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +HA+V+ +    +  + ++L+ MY +     DA  LF  M  +D V+W+ ++ G      +G   N F   R    SG  P+  TL  V+  C DM  L  G+ +H +V+K G+        +L++MY K   +D A QLF++M  R LV+W  M+        A++ + LF+ M   G+ PD+  MV ++ AC   G   +A  +H YI R  F+ D+I+ TA++++YAK G ++++ +IF+ ++ ++ I W+AM+A Y  H  GR+AI +F LM+  GV  + +TF  LL ACSHSGL+EEG + F +M + + V   + HY+CMVDLLGR+GRL++A EL++SM +E    +WGALLGACR++GN++L ++    LL L P +  +Y++LSNIY+ AG W+D +K+R LM +RRL + PG ++IE  NK ++F  GD++HP+S++I+  LE L +K+  AG  P T FVLHD+DEEVK+ M+  HSEKLAIAFGL+ T  G P+ ITKNLR+CGDCH+  KF S + +R II+RD  RFHHF +G CSC DYW
Sbjct:   84 IHARVIKSLNYSDGFIGDRLVSMYFKLGYDEDAQRLFDEMPNKDLVSWNSLMSGLSGRGYLGACLNAFCRMRT--ESGRQPNEVTLLSVVSACADMGALDEGKSLHGVVVKLGMSGKAKVVNSLINMYGKLGFLDAASQLFEEMPVRSLVSWNSMVVIHNHNGYAEKGMDLFNLMKRAGINPDQATMVALLRACTDTGLGRQAESIHAYIHRCGFNADIIIATALLNLYAKLGRLNASEDIFEEIKDRDRIAWTAMLAGYAVHACGREAIKLFDLMVKEGVEVDHVTFTHLLSACSHSGLVEEGKKYFEIMSEVYRVEPRLDHYSCMVDLLGRSGRLEDAYELIKSMPMEPSSGVWGALLGACRVYGNVELGKEVAEQLLSLDPSDHRNYIMLSNIYSAAGLWRDASKVRALMKERRLTRNPGCSFIEHGNKIHRFVVGDQLHPRSDEIHTKLEELIRKIREAGCAPKTEFVLHDIDEEVKVDMINKHSEKLAIAFGLLVTGSGVPLIITKNLRICGDCHSTAKFASLLEKRTIIIRDSKRFHHFADGLCSCRDYW 637          

HSP 2 Score: 71.633 bits (174), Expect = 9.939e-13
Identity = 54/226 (23.89%), Postives = 107/226 (47.35%), Query Frame = 1

Query:  559 TIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGH-GRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLM 1233
            ++V A +   ++     +H  + ++    D  +G  ++ MY K G  + A+ +FD M  K++++W+++++     G+ G    A   +   SG  PN +T +S++ AC+  G ++EG  +  ++  +  +    K    ++++ G+ G LD A +L E M V +    W +++    IH +   AEK ++               L N+  RAG   D A M  L+
Sbjct:   67 SLVFAISSCTSVSYCSAIHARVIKSLNYSDGFIGDRLVSMYFKLGYDEDAQRLFDEMPNKDLVSWNSLMSGLSGRGYLGACLNAFCRMRTESGRQPNEVTLLSVVSACADMGALDEGKSLHGVV-VKLGMSGKAKVVNSLINMYGKLGFLDAASQLFEEMPV-RSLVSWNSMV---VIHNHNGYAEKGMD---------------LFNLMKRAGINPDQATMVALL 272          

BLAST of ppa025850m vs. Vitis vinifera
Match: GSVIVT01033004001 (assembled CDS)

HSP 1 Score: 483.797 bits (1244), Expect = 8.390e-137
Identity = 244/539 (45.27%), Postives = 326/539 (60.48%), Query Frame = 1

Query:  142 TWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVP--------------------------------DKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +W+ ++      GD       F  + +  + P+  T P  I+ C  + DL  GR  H   L  G   D F  +ALVDMY+KC  + DAR LFD++ +R++V+WT MI  + +  +A  +L +FD M E  V+                                 + V +  ++ ACA  G+    + +H  + +     +V +GT++IDMY KCG ++ AR+ FDRM+ KNV +WSAM+A YG HGH ++A+ +F+ M  +GV PN ITFVS+L ACSH+GL+EEG   F  M  EF V   ++HY CMVDLLGRAG L EA +L++ M +  D  +WGALLGACR+H N+DL E +   L EL P+N G+YVLLSNIYA AGRW+DV +MR LM    L K PG + +++  + + F  GDR HPQ EKIY  LE L  KL+  GYVPD   VLHDV  E K  +L  HSEKLA+AFG++ T  GT I I KNLRVCGDCHT  KF+S +  R I+VRD  RFHHFR+G CSCGDYW
Sbjct:   43 SWNSVIAELARSGDSVEALRAFSSMRKLSLKPNRSTFPCAIKSCSALLDLHSGRQAHQQALIFGFEPDLFVSSALVDMYSKCGELRDARTLFDEISHRNIVSWTSMITGYVQNDDAHRALRVFDGMAERDVISWNSIIAVYAQNGMSTESMEIFHRMVKDGEINYNAVTLSAVLLACAHSGSQRLGKCIHDQVIKMGLESNVFVGTSIIDMYCKCGKVEMARKAFDRMREKNVKSWSAMVAGYGMHGHAKEALEVFYEMNMAGVKPNYITFVSVLAACSHAGLLEEGWHWFKAMSHEFDVEPGVEHYGCMVDLLGRAGYLKEAFDLIKGMKLRPDFVVWGALLGACRMHKNVDLGEISARKLFELDPKNCGYYVLLSNIYADAGRWEDVERMRILMKNSGLVKPPGFSLVDIKGRVHVFLVGDREHPQHEKIYEYLEKLSMKLQEVGYVPDMTSVLHDVGHEEKEMVLRVHSEKLAVAFGIMNTVPGTTIHIIKNLRVCGDCHTAIKFISKIVDREIVVRDSKRFHHFRDGLCSCGDYW 581          

HSP 2 Score: 95.9005 bits (237), Expect = 4.921e-20
Identity = 65/276 (23.55%), Postives = 134/276 (48.55%), Query Frame = 1

Query:  412 LFDKMQNR-DLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAM-------------------------------IAAYGYHGHGRKAIAIFHLMLNSG-VSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLEL 1140
            LF+K  ++ ++ +W  +I   A   ++ E+L  F  M +  + P++      + +C+ L  +H  R  H       F  D+ + +A++DMY+KCG +  AR +FD +  +N+++W++M                               IA Y  +G   +++ IFH M+  G ++ N +T  ++L AC+HSG    G  I   +  +  + +++   T ++D+  + G+++ A +  + M  EK+ + W A++    +HG+   A++A+    E+
Sbjct:   31 LFNKYVDKTNVFSWNSVIAELARSGDSVEALRAFSSMRKLSLKPNRSTFPCAIKSCSALLDLHSGRQAHQQALIFGFEPDLFVSSALVDMYSKCGELRDARTLFDEISHRNIVSWTSMITGYVQNDDAHRALRVFDGMAERDVISWNSIIAVYAQNGMSTESMEIFHRMVKDGEINYNAVTLSAVLLACAHSGSQRLGKCIHDQV-IKMGLESNVFVGTSIIDMYCKCGKVEMARKAFDRMR-EKNVKSWSAMVAGYGMHGH---AKEALEVFYEM 301          

HSP 3 Score: 60.8474 bits (146), Expect = 1.754e-9
Identity = 44/169 (26.04%), Postives = 78/169 (46.15%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMG-----RLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNR-DLVTWTVMIGAFAECRNAD 492
            +H QV+  G+  N+ V   ++ MY +   +  A   F  M E++  +WS MV G+   G        F E+  +GV P+  T   V+  C     L+ G      + H+  ++ G+  +++ C  +VD+  +   + +A  L   M+ R D V W  ++GA    +N D
Sbjct:  231 IHDQVIKMGLESNVFVGTSIIDMYCKCGKVEMARKAFDRMREKNVKSWSAMVAGYGMHGHAKEALEVFYEMNMAGVKPNYITFVSVLAACSHAGLLEEGWHWFKAMSHEFDVEPGV--EHYGC--MVDLLGRAGYLKEAFDLIKGMKLRPDFVVWGALLGACRMHKNVD 395          

BLAST of ppa025850m vs. Vitis vinifera
Match: GSVIVT01032394001 (assembled CDS)

HSP 1 Score: 481.1 bits (1237), Expect = 5.438e-136
Identity = 253/598 (42.31%), Postives = 355/598 (59.36%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIR--SGVT--------PDIYTLPFVIRVCRDMKDL----------------------------KMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEG-------VVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +H  VV +G   N+ V   L+ +Y++   LGDA  LF GM  RD V WS M+  +   G        F ++I+   G+T        P +   P  I  C     +                            +   +    +  +GL  D+        MY     +D A  +F+ + N     W VMI  FA       SL L+  M+E+G       V+P++V++++++ AC  LGA+ +    H Y+ +  F  D+++ TA++DMY+KCG +D AR +FD    K+++ WSAMIA+YG HGHGRKAI +F  M+ +GV P+ +TF  +L ACSHSGL+EEG   F LM +EF +   + +Y CMVDLLGRAG+L EA++L+E+M VE D  +WG+LLGACRIH N+DLAEK  + L  L P +AG++VLLSNIYA   RW +V K+R +M++R   K+ G + +E DN+ ++F  GDR HPQ EK+YA LE L   ++  GYVP T+FVLHD++EE K A L  HSE+LAIAFGLI T+ GT +RITKNLR+CGDCH   K +S +  RVI+VRD++RFH F +G CSCGDYW
Sbjct:  360 LHGYVVRSGFNSNVFVGASLIELYSKCGSLGDAVKLFKGMIVRDVVIWSSMIAAYGIHGRGGEALEIFDQMIQVMQGITSCYQISMQPQVQP-PLAITSCTLATHIPWKVKAFYMRAHFRWLGHFWEIFPTYPFQAADMSKSNIFAYGLQYDSRILTKFAIMYVSFNRIDAASIVFEDIPNPCSFLWNVMIRGFATDGRFLSSLELYSKMMEKGLKPDNSGVIPNRVSILSVLLACGNLGALRKGEWFHSYVIQTGFEFDILVATAIMDMYSKCGSLDLARCLFDETAGKDLVCWSAMIASYGIHGHGRKAIDLFDQMVKAGVRPSHVTFTCVLSACSHSGLLEEGKMYFQLMTEEFVIARKLSNYACMVDLLGRAGQLSEAVDLIENMPVEPDASIWGSLLGACRIHNNLDLAEKIADHLFHLDPVHAGYHVLLSNIYAAKSRWNEVEKVRKMMARRGANKIQGFSLVEYDNQVHKFGVGDRSHPQWEKLYAKLEELAAPMKHLGYVPLTDFVLHDIEEEAKEAALSYHSERLAIAFGLINTSPGTTLRITKNLRICGDCHNAIKLISKIVNRVILVRDMHRFHRFEDGVCSCGDYW 956          

HSP 2 Score: 138.658 bits (348), Expect = 6.620e-33
Identity = 89/347 (25.65%), Postives = 166/347 (47.84%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELI-RSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLL-ADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESM 1035
            ++H+QV   G+L +   A KL  +YA+   L  A  +F      +   W+  +  +     +      F  +I  +G  PD +T+P  ++ C  ++ L++G+++H    K+  + +D F  +ALV++Y+KC  + +A ++F++ Q  D V WT M+  + +  + +E+L LF  M                                  +  + F  D+ L  +++++YAK GC   A  +F +M  K+VI+WS MIA Y  +    +A+ +FH M+     PN +T VS L AC+ S  +EEG +I  +     AV  D+  +  ++    + G   +++ +  +M
Sbjct:   22 QLHSQVFKTGILHDTFFATKLNSLYAKCASLQAARKVFDETPHPNVHLWNSTLRSYCREKQWEETLRLFHLMICTAGEAPDNFTIPIALKACAGLRMLELGKVIHGFAKKNDEIGSDMFVGSALVELYSKCGQMGEALKVFEEFQRPDTVLWTSMVTGYQQNNDPEEALALFSQM----------------------------------VMMDCFDGDLPLVNSLLNLYAKTGCEKIAANLFSKMPEKDVISWSTMIACYANNEAANEALNLFHEMIEKRFEPNSVTVVSALQACAVSRNLEEGKKIHKI-----AVWKDVVSWVALLSGYAQNGMAYKSMGVFRNM 329          

HSP 3 Score: 137.887 bits (346), Expect = 1.129e-32
Identity = 77/262 (29.39%), Postives = 135/262 (51.53%), Query Frame = 1

Query:   40 NLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLML 825
            +L + N LL +YA+      A  LF  M E+D ++WS M+  + N          F E+I     P+  T+   ++ C   ++L+ G+ +H I +                                    +D+V+W  ++  +A+   A +S+ +F  M+ +G+ PD VA+V I+ A ++LG   +A  LH Y+ R+ F+ +V +G ++I++Y+KCG +  A ++F  M V++V+ WS+MIAAYG HG G +A+ IF  M+
Sbjct:  205 DLPLVNSLLNLYAKTGCEKIAANLFSKMPEKDVISWSTMIACYANNEAANEALNLFHEMIEKRFEPNSVTVVSALQACAVSRNLEEGKKIHKIAVW-----------------------------------KDVVSWVALLSGYAQNGMAYKSMGVFRNMLSDGIQPDAVAVVKILAASSELGIFQQALCLHGYVVRSGFNSNVFVGASLIELYSKCGSLGDAVKLFKGMIVRDVVIWSSMIAAYGIHGRGGEALEIFDQMI 431          

HSP 4 Score: 110.153 bits (274), Expect = 2.522e-24
Identity = 93/381 (24.41%), Postives = 167/381 (43.83%), Query Frame = 1

Query:  130 RDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIE--EGV-------VPDKVAMVTIVNACA-------KLGAM----HRARLLHYY-----------------ICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLML-------NSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGN----IDLAEKAVNS 1128
            +D V+W  ++ G+   G        FR ++  G+ PD   +  ++    ++   +    +H  V++ G  ++ F  A+L+++Y+KC  + DA +LF  M  RD+V W+ MI A+       E+L +FD MI+  +G+       +  +V     + +C        K+ A     H   L H++                 I       D  + T    MY     ID+A  +F+ +       W+ MI  +   G    ++ ++  M+       NSGV PN ++ +S+L AC + G + +G    S +  +     DI   T ++D+  + G LD A  L +  A  KD   W A++ +  IHG+    IDL ++ V +
Sbjct:  301 KDVVSWVALLSGYAQNGMAYKSMGVFRNMLSDGIQPDAVAVVKILAASSELGIFQQALCLHGYVVRSGFNSNVFVGASLIELYSKCGSLGDAVKLFKGMIVRDVVIWSSMIAAYGIHGRGGEALEIFDQMIQVMQGITSCYQISMQPQVQPPLAITSCTLATHIPWKVKAFYMRAHFRWLGHFWEIFPTYPFQAADMSKSNIFAYGLQYDSRILTKFAIMYVSFNRIDAASIVFEDIPNPCSFLWNVMIRGFATDGRFLSSLELYSKMMEKGLKPDNSGVIPNRVSILSVLLACGNLGALRKGEWFHSYV-IQTGFEFDILVATAIMDMYSKCGSLDLARCLFDETA-GKDLVCWSAMIASYGIHGHGRKAIDLFDQMVKA 679          

HSP 5 Score: 107.071 bits (266), Expect = 2.135e-23
Identity = 76/316 (24.05%), Postives = 149/316 (47.15%), Query Frame = 1

Query:  307 VHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMI-EEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRN-QFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFV-SLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESM---AVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMS 1236
            +H  V K G+L D F    L  +YAKC  +  AR++FD+  + ++  W   + ++   +  +E+L LF  MI   G  PD   +   + ACA L  +   +++H +  +N +   D+ +G+A++++Y+KCG +  A ++F+  Q  + + W++M+  Y  +    +A+A+F  M+        +  V SLL   + +G  +    +FS M ++     D+  ++ M+         +EAL L   M     E +     + L AC +  N++  +K     + +  +      LLS        +K +   R+++S
Sbjct:   23 LHSQVFKTGILHDTFFATKLNSLYAKCASLQAARKVFDETPHPNVHLWNSTLRSYCREKQWEETLRLFHLMICTAGEAPDNFTIPIALKACAGLRMLELGKVIHGFAKKNDEIGSDMFVGSALVELYSKCGQMGEALKVFEEFQRPDTVLWTSMVTGYQQNNDPEEALALFSQMVMMDCFDGDLPLVNSLLNLYAKTGCEKIAANLFSKMPEK-----DVISWSTMIACYANNEAANEALNLFHEMIEKRFEPNSVTVVSALQACAVSRNLEEGKKI--HKIAVWKDVVSWVALLSGYAQNGMAYKSMGVFRNMLS 331          

HSP 6 Score: 70.0922 bits (170), Expect = 2.892e-12
Identity = 49/214 (22.90%), Postives = 101/214 (47.20%), Query Frame = 1

Query:  610 LHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLML-NSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVL--LSNIYARAGRWKDVAKMRDLMSQR 1242
            LH  + +     D    T +  +YAKC  + +AR++FD     NV  W++ + +Y       + + +FHLM+  +G +P+  T    L AC+   ++E G  I         + +D+   + +V+L  + G++ EAL++ E      D  LW +++   + + + + A    + ++ +   +    ++  L N+YA+ G  K  A +   M ++
Sbjct:   23 LHSQVFKTGILHDTFFATKLNSLYAKCASLQAARKVFDETPHPNVHLWNSTLRSYCREKQWEETLRLFHLMICTAGEAPDNFTIPIALKACAGLRMLELGKVIHGFAKKNDEIGSDMFVGSALVELYSKCGQMGEALKVFEEFQ-RPDTVLWTSMVTGYQQNNDPEEALALFSQMVMMDCFDGDLPLVNSLLNLYAKTGCEKIAANLFSKMPEK 235          

BLAST of ppa025850m vs. Vitis vinifera
Match: GSVIVT01008173001 (assembled CDS)

HSP 1 Score: 480.33 bits (1235), Expect = 9.276e-136
Identity = 227/554 (40.97%), Postives = 349/554 (63.00%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYA-LFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +H       ++ ++ V N L+ MY +  ++ +A   +F  M  +   +W+ ++ G+    D       + ++  SG+ PD +T+  ++  C  MK L  G  +H   L++GL  D F   +L+ +Y  C     A+ LFD M++R LV+W VMI  +++    DE++ LF  M+ +G+ P ++A++ +  AC++L A+   + LH +  +   + D+ + +++IDMYAK GCI  ++ IFDR++ K+V +W+ +IA YG HG G++A+ +F  ML  G+ P+  TF  +L ACSH+GL+E+GL  F+ M +   +   ++HYTC+VD+LGRAGR+D+AL L+E M  + D R+W +LL +CRIHGN+ L EK  N LLEL+PE   +YVL+SN++A +G+W DV ++R  M    L+K  G +WIEV  K + F  GD + P+ E++      L  K+   GY PDT  VLHD++EE K+ +L  HSEKLAI+FGL+ TA G P+R+ KNLR+CGDCH   KF+S V  R I+VRD  RFHHFR+G CSCGDYW
Sbjct:  230 IHGMATKMDLVSDVFVGNALIAMYGKCGLVEEAVKRVFDLMDTKTVSSWNALLCGYAQNSDPRKALDLYLQMTDSGLDPDWFTIGSLLLACSRMKSLHYGEEIHGFALRNGLAVDPFIGISLLSLYICCGKPFAAQVLFDGMEHRSLVSWNVMIAGYSQNGLPDEAINLFRQMLSDGIQPYEIAIMCVCGACSQLSALRLGKELHCFALKAHLTEDIFVSSSIIDMYAKGGCIGLSQRIFDRLREKDVASWNVIIAGYGIHGRGKEALELFEKMLRLGLKPDDFTFTGILMACSHAGLVEDGLEYFNQMLNLHNIEPKLEHYTCVVDMLGRAGRIDDALRLIEEMPGDPDSRIWSSLLSSCRIHGNLGLGEKVANKLLELEPEKPENYVLISNLFAGSGKWDDVRRVRGRMKDIGLQKDAGCSWIEVGGKVHNFLIGDEMLPELEEVRETWRRLEVKISSIGYTPDTGSVLHDLEEEDKIGILRGHSEKLAISFGLLNTAKGLPVRVYKNLRICGDCHNAAKFISKVVNRDIVVRDNKRFHHFRDGICSCGDYW 783          

HSP 2 Score: 169.859 bits (429), Expect = 2.682e-42
Identity = 114/448 (25.45%), Postives = 220/448 (49.11%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVAN-KLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIR-SGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDA-RQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIE---------------EGLRIFSLMWDEFAV---------------RADIKHYTCMVDLLGRAGRLDEALELVESM---AVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQ--PENAGHYVLLSNIYARAGRWKDVAKMRDLM 1233
            +H  V  +    N  V N +++ MY+      D+  +F  +  ++   W+ +V  +     F +  + F ELI  +   PD +TLP VI+ C  + DL +G+++H +  K  L++D F   AL+ MY KC +V++A +++FD M  + + +W  ++  +A+  +  ++L L+  M + G+ PD   + +++ AC+++ ++H    +H +  RN  ++D  +G +++ +Y  CG   +A+ +FD M+ +++++W+ MIA Y  +G   +AI +F  ML+ G+ P  I  + +  ACS    +                E + + S + D +A                  D+  +  ++   G  GR  EALEL E M    ++ D+  +  +L AC   G ++   +  N +L L        HY  + ++  RAGR  D  ++ + M
Sbjct:  127 LHEMVSASTQFCNDFVLNTRIITMYSMCGSPSDSRMVFDKLRRKNLFQWNAIVSAYTRNELFEDAMSIFSELISVTEHKPDNFTLPCVIKACAGLLDLGLGQIIHGMATKMDLVSDVFVGNALIAMYGKCGLVEEAVKRVFDLMDTKTVSSWNALLCGYAQNSDPRKALDLYLQMTDSGLDPDWFTIGSLLLACSRMKSLHYGEEIHGFALRNGLAVDPFIGISLLSLYICCGKPFAAQVLFDGMEHRSLVSWNVMIAGYSQNGLPDEAINLFRQMLSDGIQPYEIAIMCVCGACSQLSALRLGKELHCFALKAHLTEDIFVSSSIIDMYAKGGCIGLSQRIFDRLREKDVASWNVIIAGYGIHGRGKEALELFEKMLRLGLKPDDFTFTGILMACSHAGLVEDGLEYFNQMLNLHNIEPKLEHYTCVVDMLGRAGRIDDALRLIEEM 574          

HSP 3 Score: 122.479 bits (306), Expect = 4.910e-28
Identity = 63/222 (28.38%), Postives = 118/222 (53.15%), Query Frame = 1

Query:  259 VIRVCRDMKDLKMGRLVHDIVLKHGLLADNFAC-AALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIE-EGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSA-REIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRI 915
            +++ C   KD+++GR +H++V       ++F     ++ MY+ C    D+R +FDK++ ++L  W  ++ A+      ++++ +F  +I      PD   +  ++ ACA L  +   +++H    +     DV +G A+I MY KCG ++ A + +FD M  K V +W+A++  Y  +   RKA+ ++  M +SG+ P+  T  SLL ACS    +  G  I
Sbjct:  111 LLQACGQRKDIEVGRRLHEMVSASTQFCNDFVLNTRIITMYSMCGSPSDSRMVFDKLRRKNLFQWNAIVSAYTRNELFEDAMSIFSELISVTEHKPDNFTLPCVIKACAGLLDLGLGQIIHGMATKMDLVSDVFVGNALIAMYGKCGLVEEAVKRVFDLMDTKTVSSWNALLCGYAQNSDPRKALDLYLQMTDSGLDPDWFTIGSLLLACSRMKSLHYGEEI 332          

HSP 4 Score: 76.6406 bits (187), Expect = 3.090e-14
Identity = 56/200 (28.00%), Postives = 106/200 (53.00%), Query Frame = 1

Query:  550 AMVTIVNACAKLGAMHRARLLHYYICRN-QFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLN-SGVSPNVITFVSLLYACSHSGLIEEGL-RIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLEL 1140
            AM  ++ AC +   +   R LH  +  + QF  D +L T +I MY+ CG    +R +FD+++ KN+  W+A+++AY  +     A++IF  +++ +   P+  T   ++ AC  +GL++ GL +I   M  +  + +D+     ++ + G+ G ++EA++ V  +   K    W ALL  C    N D   KA++  L++
Sbjct:  107 AMGVLLQACGQRKDIEVGRRLHEMVSASTQFCNDFVLNTRIITMYSMCGSPSDSRMVFDKLRRKNLFQWNAIVSAYTRNELFEDAMSIFSELISVTEHKPDNFTLPCVIKAC--AGLLDLGLGQIIHGMATKMDLVSDVFVGNALIAMYGKCGLVEEAVKRVFDLMDTKTVSSWNALL--CGYAQNSD-PRKALDLYLQM 301          

HSP 5 Score: 55.8398 bits (133), Expect = 5.644e-8
Identity = 34/160 (21.25%), Postives = 82/160 (51.25%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMG-RLVHDIVLKHGL--LADNFACAALVDMYAKCKVVDDARQLFDKMQ-NRDLVTWTVMIGA 468
            E+H   +   + +++ V++ ++ MYA+   +G +  +F  + E+D  +W+V++ G+   G        F +++R G+ PD +T   ++  C     ++ G    + ++  H +    +++ C  +VDM  +   +DDA +L ++M  + D   W+ ++ +
Sbjct:  432 ELHCFALKAHLTEDIFVSSSIIDMYAKGGCIGLSQRIFDRLREKDVASWNVIIAGYGIHGRGKEALELFEKMLRLGLKPDDFTFTGILMACSHAGLVEDGLEYFNQMLNLHNIEPKLEHYTC--VVDMLGRAGRIDDALRLIEEMPGDPDSRIWSSLLSS 589          

BLAST of ppa025850m vs. Vitis vinifera
Match: GSVIVT01019975001 (assembled CDS)

HSP 1 Score: 474.937 bits (1221), Expect = 3.897e-134
Identity = 230/522 (44.06%), Postives = 336/522 (64.37%), Query Frame = 1

Query:  100 AYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLH-DVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            A+ LF  + + D V ++ M  G+         F  F +++ SG+ PD YT P +++ C   K L+ GR +H + +K GL  + + C  L++MY  C  +D AR++FDK+    +VT+  MI  +A     +E+L LF  +    + P  V M++++++CA LGA+   + +H Y+ +N F+  V + TA+IDMYAKCG +D A  +F+ M V++   WSAMI AY  HGHG KA+++F  M  +G  P+ ITF+ LLYACSH+GL+EEG   F  M D++ V   IKHY CMVDLLGRAGRL+EA E +  + +     LW  LL AC  HGN++L ++ +  + EL   + G Y++LSN+ ARAGRW+DV  +R LM++R + K+PG + +EV+N  ++F +GD +H  S K++  L+ L K+L+L GYVP+T+ V H D+++E K   L  HSEKLAI FGL+ T  GT IR+ KNLRVCGDCH+  K +S +  R II+RDV RFHHF++G CSC DYW
Sbjct:   74 AHHLFDQIPQPDIVLFNTMARGYARTDTPLRAFTLFTQILFSGLFPDDYTFPSLLKACASCKALEEGRQLHCLAIKLGLSENVYVCPTLINMYTACNEMDCARRVFDKIWEPCVVTYNAMITGYARGSRPNEALSLFRELQARNLKPTDVTMLSVLSSCALLGALDLGKWMHEYVKKNGFNRFVKVDTALIDMYAKCGSLDDAVCVFENMAVRDTQAWSAMIMAYAIHGHGLKAVSLFKEMRKAGTEPDEITFLGLLYACSHTGLVEEGFEYFYGMRDKYGVIPGIKHYGCMVDLLGRAGRLEEAYEFIVGLPIRPTPILWRTLLSACGSHGNVELGKRVIEQIFELDDSHGGDYIILSNLCARAGRWEDVNYVRKLMNERGVVKIPGCSSVEVNNVVHEFFSGDGVHSVSTKLHQALDELVKELKLVGYVPNTSLVFHADMEDEEKEVTLRYHSEKLAITFGLLNTPPGTTIRVVKNLRVCGDCHSAAKLISLIFDRQIILRDVQRFHHFKDGKCSCEDYW 595          

BLAST of ppa025850m vs. Vitis vinifera
Match: GSVIVT01036169001 (assembled CDS)

HSP 1 Score: 473.011 bits (1216), Expect = 1.481e-133
Identity = 236/557 (42.37%), Postives = 355/557 (63.73%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQN-RDLVTWTVMIGAFAECRNADESLVLFDWMI-EEGVVPDKVAMVTIVNACAK-LGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +VH  V+ +G+   + V N ++ MY++  ++ DA A+F  M  R++V+W+ M+ GFV  G     F  F  +   GV         VI++C ++K++   + +H  V+K+G   D     AL+  Y+KC  +DDA +LF  M   +++V+WT +I  + +    D ++ LF  M  EEGV P++    +++NACA    ++ + +  H    ++ FS  + + +A++ MYAK G I+SA E+F R   +++++W++MI+ Y  HG G+K++ IF  M +  +  + ITF+ ++ AC+H+GL+ EG R F LM  ++ +   ++HY+CMVDL  RAG L++A++L+  M       +W  LL ACR+H N+ L E A   L+ LQP+++  YVLLSNIYA AG W++ AK+R LM  +++KK  G +WIEV NKT+ F  GD  HPQS++IY  LE L  +L+ AGY PDT +VLHDV+EE K  +L  HSE+LAIAFGLIAT  GTPI+I KNLRVCGDCHT  K +S +  R I+VRD NRFHHF+ G+CSCGDYW
Sbjct:  214 QVHTMVIKSGLDSTIFVGNSMVNMYSKSLMVSDAKAVFDSMENRNAVSWNSMIAGFVTNGLDLEAFELFYRMRLEGVKLTQTIFATVIKLCANIKEMSFAKQLHCQVIKNGSDFDLNIKTALMVAYSKCSEIDDAFKLFCMMHGVQNVVSWTAIISGYVQNGRTDRAMNLFCQMRREEGVEPNEFTFSSVLNACAAPTASVEQGKQFHSCSIKSGFSNALCVSSALVTMYAKRGNIESANEVFKRQVDRDLVSWNSMISGYAQHGCGKKSLKIFEEMRSKNLELDGITFIGVISACTHAGLVNEGQRYFDLMVKDYHIVPTMEHYSCMVDLYSRAGMLEKAMDLINKMPFPAGATIWRTLLAACRVHLNVQLGELAAEKLISLQPQDSAAYVLLSNIYATAGNWQERAKVRKLMDMKKVKKEAGYSWIEVKNKTFSFMAGDLSHPQSDRIYLKLEELSIRLKDAGYYPDTKYVLHDVEEEHKEVILSQHSERLAIAFGLIATPPGTPIQIVKNLRVCGDCHTVIKLISKIEGRDIVVRDSNRFHHFKGGSCSCGDYW 770          

HSP 2 Score: 125.946 bits (315), Expect = 4.441e-29
Identity = 81/326 (24.85%), Postives = 161/326 (49.39%), Query Frame = 1

Query:  205 FRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACS-----------HSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPE 1149
            F  L RSG   D  +L  V++VC  + D  +G+ VH   +K G + D     +LVDMY K + V+D  ++FD+M+ +++V+WT ++  + +    +++L LF  M  EG+ P+      ++   A  GA+ +   +H  + ++     + +G +M++MY+K   +  A+ +FD M+ +N ++W++MIA +  +G   +A  +F+ M   GV      F +++  C+           H  +I+ G         +F    D+   T ++    +   +D+A +L   M   ++   W A++     +G  D   +A+N   +++ E
Sbjct:   80 FLGLRRSGSPTDGSSLSCVLKVCGCLFDRIVGKQVHCQCIKCGFVEDVSVGTSLVDMYMKTESVEDGERVFDEMRVKNVVSWTSLLAGYRQNGLNEQALKLFSQMQLEGIKPNPFTFAAVLGGLAADGAVEKGVQVHTMVIKSGLDSTIFVGNSMVNMYSKSLMVSDAKAVFDSMENRNAVSWNSMIAGFVTNGLDLEAFELFYRMRLEGVKLTQTIFATVIKLCANIKEMSFAKQLHCQVIKNG--------SDF----DLNIKTALMVAYSKCSEIDDAFKLFCMMHGVQNVVSWTAIISGYVQNGRTD---RAMNLFCQMRRE 390          

HSP 3 Score: 93.9745 bits (232), Expect = 1.870e-19
Identity = 56/239 (23.43%), Postives = 121/239 (50.63%), Query Frame = 1

Query:  379 AKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            +K   +  ++QLFD+   + L     ++  F+      E+L LF  +   G   D  ++  ++  C  L      + +H    +  F  DV +GT+++DMY K   ++    +FD M+VKNV++W++++A Y  +G   +A+ +F  M   G+ PN  TF ++L   +  G +E+G+++ +++  +  + + I     MV++  ++  + +A  + +SM   ++   W +++     +G
Sbjct:   37 SKLSTLSHSQQLFDETPQQGLSRNNHLLFEFSRNDQNKEALNLFLGLRRSGSPTDGSSLSCVLKVCGCLFDRIVGKQVHCQCIKCGFVEDVSVGTSLVDMYMKTESVEDGERVFDEMRVKNVVSWTSLLAGYRQNGLNEQALKLFSQMQLEGIKPNPFTFAAVLGGLAADGAVEKGVQVHTMV-IKSGLDSTIFVGNSMVNMYSKSLMVSDAKAVFDSME-NRNAVSWNSMIAGFVTNG 273          

BLAST of ppa025850m vs. Vitis vinifera
Match: GSVIVT01021023001 (assembled CDS)

HSP 1 Score: 472.626 bits (1215), Expect = 1.934e-133
Identity = 241/539 (44.71%), Postives = 342/539 (63.45%), Query Frame = 1

Query:   49 VANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFAC-AALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            VA  L+ +Y +   L +A+ +F  +  ++SV W VM+ G +N  +    F  F  + RSG   D + +  +I+ C ++   K G+  H + +K   +  NF    +LVDMY KC  +D A +LF+++  RD+V W+ +I  FA    A ES+ +F  M+ + V P+ V   +IV AC+ LG++ + R +H Y+ RN   LDV   T+ IDMYAKCGCI +A  +F ++  KNV +WS MI  +G HG   +A+ +F+ M +    PN +TFVS+L ACSHSG IEEG   F  M  ++ +    +HY CMVDLLGRAG++DEAL  + +M  E     WGALLGACRIH   +LAE+    LL L+ + +G YV+LSNIYA  G W+ V K R  M ++ + K+ G T IE++ K Y FS+ DR   ++ +I ++  SL +++   GYVPD  FVLHDVD+EVK  +L  HSEKLAI FGL+ + +G PIRITKN+RVCGDCHT +KF+S +T+R II+RDV RFHH ++G CSCGDYW
Sbjct:  150 VAPALMNVYTELGSLEEAHKVFEEVPLKNSVIWGVMIKGHLNFSEEFGVFELFSRMRRSGFELDPFVVEGLIQACGNVYAGKEGKTFHGLCIKKNFIDSNFFLQTSLVDMYMKCGFLDFALKLFEEISYRDVVVWSAIIAGFARNGRALESISMFRQMLADSVTPNSVTFASIVLACSSLGSLKQGRSVHGYMIRNGVELDVKNYTSFIDMYAKCGCIVTAYRVFCQIPEKNVFSWSTMINGFGMHGLCAEALNLFYEMRSVNQLPNSVTFVSVLSACSHSGRIEEGWSHFKSMSRDYGITPVEEHYACMVDLLGRAGKIDEALSFINNMPTEPGASAWGALLGACRIHRRAELAEEVAKKLLPLESDQSGVYVMLSNIYADVGMWEMVKKTRLKMCEKGIHKIVGFTSIEIEEKLYLFSSEDRFAYKNTQIESLWNSLKERMRELGYVPDLRFVLHDVDDEVKQEVLCGHSEKLAIVFGLLNSGEGMPIRITKNMRVCGDCHTASKFISLITRRKIIMRDVKRFHHVQDGVCSCGDYW 688          

HSP 2 Score: 159.844 bits (403), Expect = 2.775e-39
Identity = 97/385 (25.19%), Postives = 187/385 (48.57%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLH-YYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPEN 1152
            +++AQ++VN + ++L     +   Y Q   L  A   F  +   +  +W+ ++        F +    F+ +++ G   D + L F ++ C  +   +  +L H + +K  L  D +   AL+++Y +   +++A ++F+++  ++ V W VMI              LF  M   G   D   +  ++ AC  + A    +  H   I +N    +  L T+++DMY KCG +D A ++F+ +  ++V+ WSA+IA +  +G   ++I++F  ML   V+PN +TF S++ ACS  G +++G  +   M     V  D+K+YT  +D+  + G +  A   V     EK+   W  ++    +HG   L  +A+N   E++  N
Sbjct:   33 QLNAQILVNALHRSLLFGPMIFGAYIQLGSLHVASKAFNHITFENLHSWNTILASHSKNKCFYDVLQLFKRMLKEGKLVDSFNLVFAVKACFGLSLFQGAKLFHSLAIKLRLEGDPYVAPALMNVYTELGSLEEAHKVFEEVPLKNSVIWGVMIKGHLNFSEEFGVFELFSRMRRSGFELDPFVVEGLIQACGNVYAGKEGKTFHGLCIKKNFIDSNFFLQTSLVDMYMKCGFLDFALKLFEEISYRDVVVWSAIIAGFARNGRALESISMFRQMLADSVTPNSVTFASIVLACSSLGSLKQGRSVHGYMIRN-GVELDVKNYTSFIDMYAKCGCIVTAYR-VFCQIPEKNVFSWSTMINGFGMHG---LCAEALNLFYEMRSVN 412          

HSP 3 Score: 111.694 bits (278), Expect = 8.667e-25
Identity = 76/284 (26.76%), Postives = 130/284 (45.77%), Query Frame = 1

Query:   40 NLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILG--TAMIDMYAKCGCIDSAREIFDRMQVK-NVITWSAMIAAYGYHGHGRKA--IAIFHLMLNSGVSPNVITFVSLLYA 876
            N  +   L+ MY +   L  A  LF  +  RD V WS ++ GF   G      + FR+++   VTP+  T   ++  C  +  LK GR VH  ++++G+  D     + +DMYAKC  +  A ++F ++  +++ +W+ MI  F       E+L LF  M     +P+ V  V++++AC+  G +      H+      + +  +      M+D+  + G ID A    + M  +     W A++ A   H     A  +A   L L S  S  V   +S +YA
Sbjct:  249 NFFLQTSLVDMYMKCGFLDFALKLFEEISYRDVVVWSAIIAGFARNGRALESISMFRQMLADSVTPNSVTFASIVLACSSLGSLKQGRSVHGYMIRNGVELDVKNYTSFIDMYAKCGCIVTAYRVFCQIPEKNVFSWSTMINGFGMHGLCAEALNLFYEMRSVNQLPNSVTFVSVLSACSHSGRIEEG-WSHFKSMSRDYGITPVEEHYACMVDLLGRAGKIDEALSFINNMPTEPGASAWGALLGACRIHRRAELAEEVAKKLLPLESDQS-GVYVMLSNIYA 530          

BLAST of ppa025850m vs. Vitis vinifera
Match: GSVIVT01000420001 (assembled CDS)

HSP 1 Score: 471.085 bits (1211), Expect = 5.628e-133
Identity = 237/553 (42.86%), Postives = 351/553 (63.47%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            VH Q+   G   ++ V N L+ +YA+   +  A  +F G+ +R+ V+W+ M+ G+   G        F ++ +  V PD   L  V+R   D++DL+ G+ +H  V+K GL  +     +L  MYAKC  V  AR  FD+M+  +++ W  MI  +A+    +E++ LF  MI + +  D + + + + ACA++G++  A+ +  YI + ++  DV + TA+IDM+AKCG +D ARE+FDR   K+V+ WSAMI  YG HG G+ AI +F+ M  +GV PN +TFV LL AC+HSGL+EEG  +F  M   + + A  +HY C+VDLLGR+G L+EA + + +M +E    +WGALLGAC+I+ ++ L E A   L  L P N GHYV LSN+YA +  W  VAK+R LM ++ L K  G + IE++ K   F  GD+ HP+ ++I+  LESL ++L+ AG++P    VLHD+++E K   L +HSE+LAIA+GLI+TA GT +RITKNLR C +CH+ TK +S +  R I+VRD NRFHHF+ G CSC DYW
Sbjct:  191 VHGQIFRLGFESDVFVQNGLVALYAKCGRVEQARIVFEGLDDRNIVSWTSMISGYGQNGLPMEALRIFGQMRQRNVKPDWIALVSVLRAYTDVEDLEQGKSIHGCVVKMGLEFEPDLLISLTAMYAKCGQVMVARSFFDQMEIPNVMMWNAMISGYAKNGYTNEAVGLFQEMISKNIRTDSITVRSAILACAQVGSLDLAKWMGDYINKTEYRNDVFVNTALIDMFAKCGSVDLAREVFDRTLDKDVVVWSAMIVGYGLHGRGQDAIDLFYAMKQAGVCPNDVTFVGLLTACNHSGLVEEGWELFHSM-KYYGIEARHQHYACVVDLLGRSGHLNEAYDFITTMPIEPGVSVWGALLGACKIYRHVTLGEYAAEQLFSLDPFNTGHYVQLSNLYASSRLWDSVAKVRILMREKGLSKDLGYSLIEINGKLQAFRVGDKSHPRFKEIFEELESLERRLKEAGFIPHIESVLHDLNQEEKEETLCNHSERLAIAYGLISTAPGTTLRITKNLRACINCHSATKLISKLVNREIVVRDANRFHHFKNGVCSCRDYW 742          

HSP 2 Score: 184.882 bits (468), Expect = 8.056e-47
Identity = 101/365 (27.67%), Postives = 198/365 (54.25%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            ++HAQ+VV+G++++  +  K +        +G A  +F    E     W+ ++ G+ +   F +    +  +  SGV PD +TLP V++ C  +  L++G+ VH  + + G  +D F    LV +YAKC  V+ AR +F+ + +R++V+WT MI  + +     E+L +F  M +  V PD +A+V+++ A   +  + + + +H  + +     +  L  ++  MYAKCG +  AR  FD+M++ NV+ W+AMI+ Y  +G+  +A+ +F  M++  +  + IT  S + AC+  G ++    +   + ++   R D+   T ++D+  + G +D A E+ +   ++KD  +W A++    +HG
Sbjct:   89 QIHAQLVVSGLVESGFLVTKFVNASWNIGEIGYARKVFDEFPEPSVFLWNAIIRGYSSHNFFGDAIEMYSRMQASGVNPDGFTLPCVLKACSGVPVLEVGKRVHGQIFRLGFESDVFVQNGLVALYAKCGRVEQARIVFEGLDDRNIVSWTSMISGYGQNGLPMEALRIFGQMRQRNVKPDWIALVSVLRAYTDVEDLEQGKSIHGCVVKMGLEFEPDLLISLTAMYAKCGQVMVARSFFDQMEIPNVMMWNAMISGYAKNGYTNEAVGLFQEMISKNIRTDSITVRSAILACAQVGSLDLAKWMGDYI-NKTEYRNDVFVNTALIDMFAKCGSVDLAREVFD-RTLDKDVVVWSAMIVGYGLHG 451          

BLAST of ppa025850m vs. ExPASy TrEMBL
Match: I1JGM4_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 859.366 bits (2219), Expect = 0.000e+0
Identity = 411/554 (74.19%), Postives = 479/554 (86.46%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +VHA VV NG LQ+L +ANKLLY YAQ + + DAY+LF G+  RDS TWSVMVGGF   GD    +ATFREL+R GVTPD YTLPFVIR CRD  DL++GR++HD+VLKHGLL+D+F CA+LVDMYAKC VV+DA++LF++M ++DLVTWTVMIGA+A+C NA ESLVLFD M EEGVVPDKVAMVT+VNACAKLGAMHRAR  + YI RN FSLDVILGTAMIDMYAKCG ++SARE+FDRM+ KNVI+WSAMIAAYGYHG G+ AI +FH+ML+  + PN +TFVSLLYACSH+GLIEEGLR F+ MW+E AVR D+KHYTCMVDLLGRAGRLDEAL L+E+M VEKDERLW ALLGACRIH  ++LAEKA NSLLELQP+N GHYVLLSNIYA+AG+W+ VAK RD+M+QR+LKK+PG TWIEVDNKTYQFS GDR HPQS++IY ML SL KKLE+AGYVPDT+FVL DV+EEVK  MLY+HSEKLAIAFGLIA  +G PIRI+KNLRVCGDCHTF+K VS++ +R IIVRD NRFHHF +GTCSCGDYW
Sbjct:   36 QVHAHVVANGTLQDLVIANKLLYTYAQHKAIDDAYSLFDGLTMRDSKTWSVMVGGFAKAGDHAGCYATFRELLRCGVTPDNYTLPFVIRTCRDRTDLQIGRVIHDVVLKHGLLSDHFVCASLVDMYAKCIVVEDAQRLFERMLSKDLVTWTVMIGAYADC-NAYESLVLFDRMREEGVVPDKVAMVTVVNACAKLGAMHRARFANDYIVRNGFSLDVILGTAMIDMYAKCGSVESAREVFDRMKEKNVISWSAMIAAYGYHGRGKDAIDLFHMMLSCAILPNRVTFVSLLYACSHAGLIEEGLRFFNSMWEEHAVRPDVKHYTCMVDLLGRAGRLDEALRLIEAMTVEKDERLWSALLGACRIHSKMELAEKAANSLLELQPQNPGHYVLLSNIYAKAGKWEKVAKFRDMMTQRKLKKIPGWTWIEVDNKTYQFSVGDRSHPQSKEIYEMLMSLIKKLEMAGYVPDTDFVLQDVEEEVKQEMLYTHSEKLAIAFGLIAIPEGEPIRISKNLRVCGDCHTFSKMVSSIMRRSIIVRDANRFHHFNDGTCSCGDYW 588          

HSP 2 Score: 134.035 bits (336), Expect = 8.280e-29
Identity = 78/269 (29.00%), Postives = 139/269 (51.67%), Query Frame = 1

Query:  301 RLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVR----ADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            R VH  V+ +G L D      L+  YA+ K +DDA  LFD +  RD  TW+VM+G FA+  +       F  ++  GV PD   +  ++  C     +   R++H  + ++    D  +  +++DMYAKC  ++ A+ +F+RM  K+++TW+ MI AY    +  +++ +F  M   GV P+ +  V+++ AC+  G +       +   +++ VR     D+   T M+D+  + G ++ A E+ + M  EK+   W A++ A   HG
Sbjct:   35 RQVHAHVVANGTLQDLVIANKLLYTYAQHKAIDDAYSLFDGLTMRDSKTWSVMVGGFAKAGDHAGCYATFRELLRCGVTPDNYTLPFVIRTCRDRTDLQIGRVIHDVVLKHGLLSDHFVCASLVDMYAKCIVVEDAQRLFERMLSKDLVTWTVMIGAYA-DCNAYESLVLFDRMREEGVVPDKVAMVTVVNACAKLGAMHR-----ARFANDYIVRNGFSLDVILGTAMIDMYAKCGSVESAREVFDRMK-EKNVISWSAMIAAYGYHG 296          

BLAST of ppa025850m vs. ExPASy TrEMBL
Match: A5C1S9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_014227 PE=4 SV=1)

HSP 1 Score: 853.203 bits (2203), Expect = 0.000e+0
Identity = 410/554 (74.01%), Postives = 469/554 (84.66%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +VH Q  V+GMLQNL VANKL+  Y+  R L DAY LF GM  RDSV+WSVMVGGF  VGD+ N F TFRELIR G  PD YTLPFVIR CRD+K+L+MGRL+H IV K GL  D+F CAALVDMY KC+ ++DAR LFDKM  RDLVTWTVMIG +AEC NA+ESLVLFD M EEGVVPDKVAMVT+V ACAKLGAMH+AR +  YI R +F LDVILGTAMIDM+AKCGC++SAREIFDRM+ KNVI+WSAMIAAYGYHG GRKA+ +F +ML SG+ PN IT VSLLYACSH+GL+EEGLR FS MW++++VR D+KHYTC+VDLLGRAGRLDEAL+L+ SM  EKDE LWGA LGACR H ++ LAEKA  SLLELQP+N GHY+LLSNIYA AGRW+DVAK+RDLMSQRRLKK+PG TWIEVDNK++QFS GD  HP+S++IY ML+SLG KLEL GYVPDTNFVLHDVDEE+K+ +LY+HSEKLAIAFGLIAT + TPIRI KNLRVCGDCHTF K VSA+T RVIIVRD NRFHHF+EG CSCGDYW
Sbjct:  756 QVHXQASVHGMLQNLIVANKLVXFYSYYRALDDAYGLFDGMCVRDSVSWSVMVGGFAKVGDYMNCFGTFRELIRCGARPDNYTLPFVIRACRDLKNLQMGRLIHHIVYKFGLDLDHFVCAALVDMYGKCREIEDARFLFDKMXERDLVTWTVMIGGYAECGNANESLVLFDKMREEGVVPDKVAMVTVVFACAKLGAMHKARTIDDYIQRKKFQLDVILGTAMIDMHAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFPMMLRSGILPNKITLVSLLYACSHAGLVEEGLRFFSXMWEDYSVRXDVKHYTCVVDLLGRAGRLDEALKLIXSMTXEKDEGLWGAFLGACRTHKDVXLAEKAATSLLELQPQNPGHYILLSNIYANAGRWEDVAKIRDLMSQRRLKKIPGWTWIEVDNKSHQFSVGDTTHPRSKEIYEMLKSLGNKLELVGYVPDTNFVLHDVDEELKIGILYTHSEKLAIAFGLIATPEHTPIRIIKNLRVCGDCHTFCKLVSAITGRVIIVRDANRFHHFKEGACSCGDYW 1309          

HSP 2 Score: 768.844 bits (1984), Expect = 0.000e+0
Identity = 372/512 (72.66%), Postives = 434/512 (84.77%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLR 1536
            +VHAQ  V+GML+N+ VANKL+Y Y+  R L DAY LF GM  RDSV+WSVMVGGF  VGD+ N F TFRELIR G  PD YTLPFVIR CRD+K+L+MGRL+H IV K GL  D+F CAALVDMY KC+ ++DAR LFDKMQ RDLVTWTVMIG +AEC  A+ESLVLF+ M EEGVVPDKVAMVT+V ACAKLGAMH+AR++  YI R +F LDVILGTAMIDMYAKCGC++SAREIFDRM+ KNVI+WSAMIAAYGYHG GRKA+ +F +ML+SG+ P+ IT  SLLYACSH+GL+EEGLR FS MW++++VR D+KHYTC+VDLLGRAGRLDEAL+L++SM +EKDE LWGA LGACR H ++ LAEKA  SLLELQ +N GHYVLLSNIYA AGRW+DVAK+RDLMSQRRLKK PG TWIEVDNK++QFS GD  HP+S++IY ML+SL  KLEL GYVPDTNFVLHDVDEE+K+ +LY+HSEKLAIAFGLIAT + TPIRI KNLR
Sbjct:   80 QVHAQASVHGMLENIVVANKLIYFYSYYRALDDAYGLFDGMCVRDSVSWSVMVGGFAKVGDYINCFGTFRELIRCGARPDNYTLPFVIRACRDLKNLQMGRLIHHIVYKFGLDLDHFVCAALVDMYVKCREIEDARFLFDKMQERDLVTWTVMIGGYAECGKANESLVLFEKMREEGVVPDKVAMVTVVFACAKLGAMHKARIIDDYIQRKKFQLDVILGTAMIDMYAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFPMMLSSGMLPDKITLASLLYACSHAGLVEEGLRFFSSMWEDYSVRTDVKHYTCVVDLLGRAGRLDEALKLIKSMTIEKDEGLWGAFLGACRTHKDVVLAEKAATSLLELQSQNPGHYVLLSNIYANAGRWEDVAKIRDLMSQRRLKKTPGWTWIEVDNKSHQFSVGDTTHPRSKEIYEMLKSLSNKLELVGYVPDTNFVLHDVDEELKIGILYTHSEKLAIAFGLIATPEHTPIRIIKNLR 591          

HSP 3 Score: 150.984 bits (380), Expect = 6.546e-34
Identity = 85/280 (30.36%), Postives = 144/280 (51.43%), Query Frame = 1

Query:  256 FVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            F I    + ++L   R VH     HG+L +      L+  Y+  + +DDA  LFD M  RD V+W+VM+G FA+  +       F  +I  G  PD   +  ++ AC  L  +   RL+H+ + +    LD  +  A++DMY KC  I+ AR +FD+MQ ++++TW+ MI  Y   G   +++ +F  M   GV P+ +  V++++AC+  G + +  RI          + D+   T M+D+  + G ++ A E+ + M  EK+   W A++ A   HG
Sbjct:   64 FYISALVNCRNLTQVRQVHAQASVHGMLENIVVANKLIYFYSYYRALDDAYGLFDGMCVRDSVSWSVMVGGFAKVGDYINCFGTFRELIRCGARPDNYTLPFVIRACRDLKNLQMGRLIHHIVYKFGLDLDHFVCAALVDMYVKCREIEDARFLFDKMQERDLVTWTVMIGGYAECGKANESLVLFEKMREEGVVPDKVAMVTVVFACAKLGAMHKA-RIIDDYIQRKKFQLDVILGTAMIDMYAKCGCVESAREIFDRME-EKNVISWSAMIAAYGYHG 341          

HSP 4 Score: 147.132 bits (370), Expect = 9.452e-33
Identity = 84/274 (30.66%), Postives = 142/274 (51.82%), Query Frame = 1

Query:  286 DLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVR----ADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            +L   R VH     HG+L +      LV  Y+  + +DDA  LFD M  RD V+W+VM+G FA+  +       F  +I  G  PD   +  ++ AC  L  +   RL+H+ + +    LD  +  A++DMY KC  I+ AR +FD+M  ++++TW+ MI  Y   G+  +++ +F  M   GV P+ +  V++++AC+  G + +   I     D++  R     D+   T M+D+  + G ++ A E+ + M  EK+   W A++ A   HG
Sbjct:  750 NLTQVRQVHXQASVHGMLQNLIVANKLVXFYSYYRALDDAYGLFDGMCVRDSVSWSVMVGGFAKVGDYMNCFGTFRELIRCGARPDNYTLPFVIRACRDLKNLQMGRLIHHIVYKFGLDLDHFVCAALVDMYGKCREIEDARFLFDKMXERDLVTWTVMIGGYAECGNANESLVLFDKMREEGVVPDKVAMVTVVFACAKLGAMHKARTI-----DDYIQRKKFQLDVILGTAMIDMHAKCGCVESAREIFDRME-EKNVISWSAMIAAYGYHG 1017          

BLAST of ppa025850m vs. ExPASy TrEMBL
Match: B9IIT4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_777414 PE=4 SV=1)

HSP 1 Score: 795.808 bits (2054), Expect = 0.000e+0
Identity = 383/554 (69.13%), Postives = 455/554 (82.13%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +VHAQV   G++ +L VANKLLYM A+ + L  A+ LF  M ERD V+WSVM+GGFV  GD+   F TFRELIR+G  PD ++LPFVI+ CRD   L MGRL+H  VLK+GL  DNF C+ LVDMYAKC ++D+A+QLFD+M  +DLVT TVMI  +AEC   +ES VLFD M  +G VPDKVAMVTIVNACAKLGAM++ARL+H Y+C  ++SLDV LGTAMIDMYAKCG IDS+REIFDRM+ KNVI+WSAMI AYGYHG GR+A+ +FH+MLNSG+ PN ITF+SLLYACSH+GL+++GL++FSLM   + VR D+KHYTCMVDLLGRAGRLD+AL L+E+M VEKDE +W A LGACRIH  +DLAEKA   LL LQ +N GHY+LLSNIYA AGRWKDVAK+R+LM++RRLKK+PG TWIEVDN  Y+F  GD  H +S +IY ML+SL +KLE AGYVPDTN VLHDVDEEVKL +L++HSEKLAIAFGLIAT DGTPIRITKNLRVCGDCH+F K VSA+TQR IIVRD NRFHHF+EG CSCGDYW
Sbjct:   21 QVHAQVTTTGIIHDLIVANKLLYMCAKHKDLVTAHLLFNKMEERDPVSWSVMIGGFVKNGDYERCFQTFRELIRAGSKPDNFSLPFVIKACRDTMGLIMGRLIHSTVLKNGLHLDNFVCSTLVDMYAKCGMIDNAKQLFDRMPKKDLVTRTVMIAGYAECGKPNESWVLFDQMRRDGFVPDKVAMVTIVNACAKLGAMNKARLVHDYVCARRYSLDVELGTAMIDMYAKCGSIDSSREIFDRMEQKNVISWSAMIGAYGYHGQGREALELFHMMLNSGIIPNRITFISLLYACSHAGLVDDGLQLFSLMSVSYGVRPDVKHYTCMVDLLGRAGRLDQALRLIENMEVEKDEGIWCAFLGACRIHRQVDLAEKAAKLLLSLQTQNPGHYILLSNIYANAGRWKDVAKIRNLMAKRRLKKIPGYTWIEVDNIIYRFGAGDNSHLRSNEIYEMLKSLSQKLESAGYVPDTNSVLHDVDEEVKLGILHAHSEKLAIAFGLIATPDGTPIRITKNLRVCGDCHSFCKLVSAITQRDIIVRDANRFHHFKEGICSCGDYW 574          

BLAST of ppa025850m vs. ExPASy TrEMBL
Match: F6HKM7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0007g03070 PE=4 SV=1)

HSP 1 Score: 672.929 bits (1735), Expect = 0.000e+0
Identity = 321/413 (77.72%), Postives = 364/413 (88.14%), Query Frame = 1

Query:  424 MQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            M  RDLVTWTVMIG +AEC NA+ESLVLFD M EEGVVPDKVAMVT+V ACAKLGAMH+AR +  YI R +F LDVILGTAMIDM+AKCGC++SAREIFDRM+ KNVI+WSAMIAAYGYHG GRKA+ +F +ML SG+ PN IT VSLLYACSH+GL+EEGLR FSLMW++++VRAD+KHYTC+VDLLGRAGRLDEAL+L+ESM VEKDE LWGA LGACR H ++ LAEKA  SLLELQP+N GHY+LLSNIYA AGRW+DVAK RDLMSQRRLKK+PG TWIEVDNK++QFS GD  HP+S++IY ML+SLG KLEL GYVPDTNFVLHDVDEE+K+ +LYSHSEKLAIAFGLIAT + TPIRI KNLRVCGDCHTF K VSA+T RVIIVRD NRFHHF+EG CSCGDYW
Sbjct:    1 MPERDLVTWTVMIGGYAECGNANESLVLFDKMREEGVVPDKVAMVTVVFACAKLGAMHKARTIDDYIQRKKFQLDVILGTAMIDMHAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFPMMLRSGILPNKITLVSLLYACSHAGLVEEGLRFFSLMWEDYSVRADVKHYTCVVDLLGRAGRLDEALKLIESMTVEKDEGLWGAFLGACRTHKDVVLAEKAATSLLELQPQNPGHYILLSNIYANAGRWEDVAKTRDLMSQRRLKKIPGWTWIEVDNKSHQFSVGDTTHPRSKEIYEMLKSLGNKLELVGYVPDTNFVLHDVDEELKIGILYSHSEKLAIAFGLIATPEHTPIRIIKNLRVCGDCHTFCKLVSAITGRVIIVRDANRFHHFKEGACSCGDYW 413          

HSP 2 Score: 126.331 bits (316), Expect = 1.727e-26
Identity = 65/224 (29.02%), Postives = 120/224 (53.57%), Query Frame = 1

Query:  121 MGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSL--DVILGTAMIDMYAKCGCIDSAREIFDRMQV-KNVITWSAMIAAYGYH 783
            M ERD VTW+VM+GG+   G+       F ++   GV PD   +  V+  C  +  +   R + D + +     D     A++DM+AKC  V+ AR++FD+M+ +++++W+ MI A+       ++L LF  M+  G++P+K+ +V+++ AC+  G +    L  + +    +S+  DV   T ++D+  + G +D A ++ + M V K+   W A + A   H
Sbjct:    1 MPERDLVTWTVMIGGYAECGNANESLVLFDKMREEGVVPDKVAMVTVVFACAKLGAMHKARTIDDYIQRKKFQLDVILGTAMIDMHAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFPMMLRSGILPNKITLVSLLYACSHAGLVEEG-LRFFSLMWEDYSVRADVKHYTCVVDLLGRAGRLDEALKLIESMTVEKDEGLWGAFLGACRTH 223          

BLAST of ppa025850m vs. ExPASy TrEMBL
Match: B9SWG6_RICCO (Bipolar kinesin KRP-130, putative OS=Ricinus communis GN=RCOM_0293150 PE=3 SV=1)

HSP 1 Score: 614.379 bits (1583), Expect = 2.090e-173
Identity = 294/428 (68.69%), Postives = 357/428 (83.41%), Query Frame = 1

Query:    1 EVHAQVVVNGML-QNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEV 1281
            ++HA +  N +L  NLTVANKLLY+Y   + L DAYALF  M +++ V+WS+M+GGFV VG+F   + TF+ELIR+GV PD YTLPFVI+ CRD   L MGRL+H +VLK+GL  D+F CAALVDMYAKCKV++DA+ LFD M ++DLVTWTVMIG +AEC NA ESLVLFD + EEG VPDK+AMV+IVNACAKLGAM++AR +H Y+ RN+FSLDVILGTAMIDMYAKCG +DSAREIFD M  KNVI+WSAMIAAYGYHG G+KA+ +F +ML+SG+ PN ITFVSLLYACSH+GL+E+GL + SLMW+ F VR DIKH+TCMVDLLGRAG+L EA   +E+M VEKDE LW +LLGACRIH  IDLAEKA  SLLELQP+N G+Y+LLSNIYA AG+WKDVAK+RDLM++R+LKK+PG TWIE+
Sbjct: 1100 QLHALITTNSVLYNNLTVANKLLYVYVHHKCLTDAYALFDEMPQKNPVSWSIMIGGFVKVGEFMQCYKTFKELIRNGVQPDNYTLPFVIKACRDTVALDMGRLIHCVVLKYGLHLDHFVCAALVDMYAKCKVIEDAKLLFDVMPSKDLVTWTVMIGGYAECGNAKESLVLFDHLREEGFVPDKIAMVSIVNACAKLGAMNKARFVHEYVNRNRFSLDVILGTAMIDMYAKCGDVDSAREIFDNMHHKNVISWSAMIAAYGYHGQGKKALELFPMMLSSGILPNNITFVSLLYACSHAGLVEDGLTLLSLMWESFGVRPDIKHHTCMVDLLGRAGQLTEAFRFIENMTVEKDEGLWSSLLGACRIHKRIDLAEKAAKSLLELQPQNPGNYILLSNIYANAGQWKDVAKIRDLMTKRKLKKIPGYTWIEL 1527          

BLAST of ppa025850m vs. ExPASy TrEMBL
Match: F6HKM6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0007g03080 PE=4 SV=1)

HSP 1 Score: 600.512 bits (1547), Expect = 3.123e-169
Identity = 285/381 (74.80%), Postives = 330/381 (86.61%), Query Frame = 1

Query:  517 MIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDY 1659
            M EEGVVPDKVAMVT+V ACAKLGAMH+AR++  YI R +F LDVILGTAMIDMYAKCGC++SAREIFDRM+ KNVI+WSAMIAAYGYHG GRKA+ +F +ML+SG+ P+ IT  SLLYACSH+GL+EEGLR FS MW++++VR D+KHYTC+VDLLGRAGRLDEA +L++SM +EKDE LWGA LGACR H ++ LAEKA  SLLELQ +N GHYVLLSNIYA AGRW+DVAK+RDLMSQRRLKK PG TWIEVDNK++QFS GD  HP+S++IY ML+SL  KLEL GYVPDTNFVLHDVDEE+K+ +LY+HSEKLAIAFGLIAT + T IRI KNLRVCGDCHTF K VS +  RVIIVRD NRFHHF+EG CSCGDY
Sbjct:    1 MREEGVVPDKVAMVTVVFACAKLGAMHKARIIDDYIQRKKFQLDVILGTAMIDMYAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFRMMLSSGMLPDKITLASLLYACSHAGLVEEGLRFFSSMWEDYSVRTDVKHYTCVVDLLGRAGRLDEAFKLIKSMTIEKDEGLWGAFLGACRTHKDVVLAEKAATSLLELQSQNPGHYVLLSNIYANAGRWEDVAKIRDLMSQRRLKKTPGWTWIEVDNKSHQFSVGDTTHPRSKEIYEMLKSLSNKLELVGYVPDTNFVLHDVDEELKIGILYTHSEKLAIAFGLIATPEHTSIRIIKNLRVCGDCHTFCKLVSEIAGRVIIVRDANRFHHFKEGACSCGDY 381          

HSP 2 Score: 97.4413 bits (241), Expect = 8.589e-18
Identity = 52/189 (27.51%), Postives = 99/189 (52.38%), Query Frame = 1

Query:  226 GVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSL--DVILGTAMIDMYAKCGCIDSAREIFDRMQV-KNVITWSAMIAAYGYH 783
            GV PD   +  V+  C  +  +   R++ D + +     D     A++DMYAKC  V+ AR++FD+M+ +++++W+ MI A+       ++L LF  M+  G++PDK+ + +++ AC+  G +    L  +      +S+  DV   T ++D+  + G +D A ++   M + K+   W A + A   H
Sbjct:    5 GVVPDKVAMVTVVFACAKLGAMHKARIIDDYIQRKKFQLDVILGTAMIDMYAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFRMMLSSGMLPDKITLASLLYACSHAGLVEEG-LRFFSSMWEDYSVRTDVKHYTCVVDLLGRAGRLDEAFKLIKSMTIEKDEGLWGAFLGACRTH 192          

BLAST of ppa025850m vs. ExPASy TrEMBL
Match: F6HUU4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0066g00420 PE=4 SV=1)

HSP 1 Score: 543.117 bits (1398), Expect = 5.917e-152
Identity = 253/509 (49.71%), Postives = 352/509 (69.16%), Query Frame = 1

Query:  136 SVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            S  W+VM+ GF   G F +    + +++  G+ PD +  PF ++ C  + DL+ G+++H  ++  G   D F  AALVDMYAKC  ++ AR +FDKM  RDLV+WT MI  +A      E+L  FD M   GV+P++V++++++ AC  LGA+ +    H Y+ +  F  D+++ TA++DMY+KCG +D AR +FD    K+++ WSAMIA+YG HGHGRKAI +F  M+ +GV P+ +TF  +L ACSHSGL+EEG   F LM +EF +   + +Y CMVDLLGRAG+L EA++L+E+M VE D  +WG+LLGACRIH N+DLAEK  + L  L P +AG++VLLSNIYA   RW +V K+R +M++R   K+ G + +E DN+ ++F  GDR HPQ EK+YA LE L   ++  GYVP T+FVLHD++EE K A L  HSE+LAIAFGLI T+ GT +RITKNLR+CGDCH   K +S +  RVI+VRD++RFH F +G CSCGDYW
Sbjct:  754 SFLWNVMIRGFATDGRFLSSLELYSKMMEKGLKPDKFAFPFALKSCAGLSDLQRGKVIHQHLVCCGCSNDLFVDAALVDMYAKCGDIEAARLVFDKMAVRDLVSWTSMISGYAHNGYNSETLGFFDLMRSSGVIPNRVSILSVLLACGNLGALRKGEWFHSYVIQTGFEFDILVATAIMDMYSKCGSLDLARCLFDETAGKDLVCWSAMIASYGIHGHGRKAIDLFDQMVKAGVRPSHVTFTCVLSACSHSGLLEEGKMYFQLMTEEFVIARKLSNYACMVDLLGRAGQLSEAVDLIENMPVEPDASIWGSLLGACRIHNNLDLAEKIADHLFHLDPVHAGYHVLLSNIYAAKSRWNEVEKVRKMMARRGANKIQGFSLVEYDNQVHKFGVGDRSHPQWEKLYAKLEELAAPMKHLGYVPLTDFVLHDIEEEAKEAALSYHSERLAIAFGLINTSPGTTLRITKNLRICGDCHNAIKLISKIVNRVILVRDMHRFHRFEDGVCSCGDYW 1262          

HSP 2 Score: 357.451 bits (916), Expect = 4.605e-96
Identity = 180/454 (39.65%), Postives = 280/454 (61.67%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLML-NSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESL 1362
            VH  V+      +L + N LL +YA+      A  LF  M E+D ++WS M+  + N          F E+I     P+  T+   ++ C   ++L+ G+ +H I +  G   D     AL+DMY KC   D+A  LF ++  +D+V+W  ++  +A+   A +S+ +F  M+ +G+ PD VA+V I+ A ++LG   +A  LH Y+ R+ F+ +V +G ++I++Y+KCG +  A ++F  M V++V+ WS+MIAAYG HG G +A+ IF  M+ NS V PN +TF+S+L ACSH+GL+EEGL+IF  M  ++ +R D +H+  MVDLLGR G+L +A++++  M +     +WGALLGACRIH NI++ E A  +L  L P +AG+Y+LLSNIYA  G+W +VA++R  + +R LKK+ G + +EV    + F   DR HP S+KIY +L  L
Sbjct:  228 VHGLVIRREFDGDLPLVNSLLNLYAKTGCEKIAANLFSKMPEKDVISWSTMIACYANNEAANEALNLFHEMIEKRFEPNSVTVVSALQACAVSRNLEEGKKIHKIAVWKGFELDFSVSTALIDMYMKCSCPDEAVDLFQRLPKKDVVSWVALLSGYAQNGMAYKSMGVFRNMLSDGIQPDAVAVVKILAASSELGIFQQALCLHGYVVRSGFNSNVFVGASLIELYSKCGSLGDAVKLFKGMIVRDVVIWSSMIAAYGIHGRGGEALEIFDQMVKNSTVRPNNVTFLSILSACSHAGLVEEGLKIFDRMVHDYQLRPDSEHFGIMVDLLGRIGQLGKAMDIINRMPIPAGPHVWGALLGACRIHHNIEMGEAAAKNLFWLDPSHAGYYILLSNIYAVDGKWDNVAELRTRIKERGLKKMFGQSMVEVRGGVHSFLASDRFHPDSQKIYELLRKL 681          

HSP 3 Score: 187.193 bits (474), Expect = 8.242e-45
Identity = 112/369 (30.35%), Postives = 200/369 (54.20%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELI-RSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLL-ADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMI-EEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSL-MWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            ++H+QV   G+L +   A KL  +YA+   L  A  +F      +   W+  +  +     +      F  +I  +G  PD +T+P  ++ C  ++ L++G+++H    K+  + +D F  +ALV++Y+KC  + +A ++F++ Q  D V WT M+  + +  + +E+L LF  M+  + VV D V +V++V+ACA+L  +     +H  + R +F  D+ L  +++++YAK GC   A  +F +M  K+VI+WS MIA Y  +    +A+ +FH M+     PN +T VS L AC+ S  +EEG +I  + +W  F +  D    T ++D+  +    DEA++L + +  +KD   W ALL     +G
Sbjct:   22 QLHSQVFKTGILHDTFFATKLNSLYAKCASLQAARKVFDETPHPNVHLWNSTLRSYCREKQWEETLRLFHLMICTAGEAPDNFTIPIALKACAGLRMLELGKVIHGFAKKNDEIGSDMFVGSALVELYSKCGQMGEALKVFEEFQRPDTVLWTSMVTGYQQNNDPEEALALFSQMVMMDCVVLDPVTLVSVVSACAQLLNVKAGSCVHGLVIRREFDGDLPLVNSLLNLYAKTGCEKIAANLFSKMPEKDVISWSTMIACYANNEAANEALNLFHEMIEKRFEPNSVTVVSALQACAVSRNLEEGKKIHKIAVWKGFEL--DFSVSTALIDMYMKCSCPDEAVDLFQRLP-KKDVVSWVALLSGYAQNG 387          

HSP 4 Score: 186.422 bits (472), Expect = 1.406e-44
Identity = 113/402 (28.11%), Postives = 209/402 (51.99%), Query Frame = 1

Query:   40 NLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSG-VTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLE---LQPENAGHYVLLSNIYARAGRWKDVAKMRDLM 1233
            ++ V + L+ +Y++   +G+A  +F      D+V W+ MV G+    D     A F +++    V  D  TL  V+  C  + ++K G  VH +V++     D     +L+++YAK      A  LF KM  +D+++W+ MI  +A    A+E+L LF  MIE+   P+ V +V+ + ACA    +   + +H       F LD  + TA+IDMY KC C D A ++F R+  K+V++W A+++ Y  +G   K++ +F  ML+ G+ P+ +  V +L A S  G+ ++ L +   +       +++     +++L  + G L +A++L + M V +D  +W +++ A  IHG    A +  + +++   ++P N     +LS   + AG  ++  K+ D M
Sbjct:  138 DMFVGSALVELYSKCGQMGEALKVFEEFQRPDTVLWTSMVTGYQQNNDPEEALALFSQMVMMDCVVLDPVTLVSVVSACAQLLNVKAGSCVHGLVIRREFDGDLPLVNSLLNLYAKTGCEKIAANLFSKMPEKDVISWSTMIACYANNEAANEALNLFHEMIEKRFEPNSVTVVSALQACAVSRNLEEGKKIHKIAVWKGFELDFSVSTALIDMYMKCSCPDEAVDLFQRLPKKDVVSWVALLSGYAQNGMAYKSMGVFRNMLSDGIQPDAVAVVKILAASSELGIFQQALCLHGYV-VRSGFNSNVFVGASLIELYSKCGSLGDAVKLFKGMIV-RDVVIWSSMIAAYGIHGRGGEALEIFDQMVKNSTVRPNNVTFLSILS-ACSHAGLVEEGLKIFDRM 536          

HSP 5 Score: 145.591 bits (366), Expect = 2.750e-32
Identity = 83/249 (33.33%), Postives = 142/249 (57.03%), Query Frame = 1

Query:  394 VDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGN----IDLAEKAVNS 1128
            +D A  +F+ + N     W VMI  FA       SL L+  M+E+G+ PDK A    + +CA L  + R +++H ++     S D+ +  A++DMYAKCG I++AR +FD+M V+++++W++MI+ Y ++G+  + +  F LM +SGV PN ++ +S+L AC + G + +G    S +  +     DI   T ++D+  + G LD A  L +  A  KD   W A++ +  IHG+    IDL ++ V +
Sbjct:  739 IDAASIVFEDIPNPCSFLWNVMIRGFATDGRFLSSLELYSKMMEKGLKPDKFAFPFALKSCAGLSDLQRGKVIHQHLVCCGCSNDLFVDAALVDMYAKCGDIEAARLVFDKMAVRDLVSWTSMISGYAHNGYNSETLGFFDLMRSSGVIPNRVSILSVLLACGNLGALRKGEWFHSYV-IQTGFEFDILVATAIMDMYSKCGSLDLARCLFDETA-GKDLVCWSAMIASYGIHGHGRKAIDLFDQMVKA 985          

HSP 6 Score: 122.479 bits (306), Expect = 2.493e-25
Identity = 76/279 (27.24%), Postives = 141/279 (50.54%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILG--TAMIDMYAKCGCIDSAREIFDRMQVK-NVITWSAMIAAYGYHGH----GRKAIAIFHL 819
            +H  +V  G   +L V   L+ MYA+   +  A  +F  M  RD V+W+ M+ G+ + G  +     F  +  SGV P+  ++  V+  C ++  L+ G   H  V++ G   D     A++DMY+KC  +D AR LFD+   +DLV W+ MI ++    +  +++ LFD M++ GV P  V    +++AC+  G +   + +++ +   +F +   L     M+D+  + G +  A ++ + M V+ +   W +++ A   H +     + A  +FHL
Sbjct:  811 IHQHLVCCGCSNDLFVDAALVDMYAKCGDIEAARLVFDKMAVRDLVSWTSMISGYAHNGYNSETLGFFDLMRSSGVIPNRVSILSVLLACGNLGALRKGEWFHSYVIQTGFEFDILVATAIMDMYSKCGSLDLARCLFDETAGKDLVCWSAMIASYGIHGHGRKAIDLFDQMVKAGVRPSHVTFTCVLSACSHSGLLEEGK-MYFQLMTEEFVIARKLSNYACMVDLLGRAGQLSEAVDLIENMPVEPDASIWGSLLGACRIHNNLDLAEKIADHLFHL 1088          

HSP 7 Score: 111.309 bits (277), Expect = 5.748e-22
Identity = 69/268 (25.75%), Postives = 130/268 (48.51%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVV-PDKVAMVTIVNACAKLGAMHRA-----RLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQV-KNVITWSAMIAAYGYH 783
            ++H   V  G   + +V+  L+ MY +     +A  LF  + ++D V+W  ++ G+   G        FR ++  G+ PD   +  ++    ++   +    +H  V++ G  ++ F  A+L+++Y+KC  + DA +LF  M  RD+V W+ MI A+       E+L +FD M++   V P+ V  ++I++AC+  G +        R++H Y    Q   D      M+D+  + G +  A +I +RM +      W A++ A   H
Sbjct:  328 KIHKIAVWKGFELDFSVSTALIDMYMKCSCPDEAVDLFQRLPKKDVVSWVALLSGYAQNGMAYKSMGVFRNMLSDGIQPDAVAVVKILAASSELGIFQQALCLHGYVVRSGFNSNVFVGASLIELYSKCGSLGDAVKLFKGMIVRDVVIWSSMIAAYGIHGRGGEALEIFDQMVKNSTVRPNNVTFLSILSACSHAGLVEEGLKIFDRMVHDY----QLRPDSEHFGIMVDLLGRIGQLGKAMDIINRMPIPAGPHVWGALLGACRIH 591          

BLAST of ppa025850m vs. ExPASy TrEMBL
Match: F5CAE0_FUNHY (Pentatricopeptide repeat protein 78 (Fragment) OS=Funaria hygrometrica PE=2 SV=1)

HSP 1 Score: 541.962 bits (1395), Expect = 1.318e-151
Identity = 260/554 (46.93%), Postives = 364/554 (65.70%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            E+H++VV +G + +++V N L+ MYA+   + DA  LF  M  +D ++W+ M+GG    G      A F+++ ++G+ P+  T   ++  C     L  GR +H  V++ GL  D      LV+MY+ C  V DARQ+FD+M  RD+V +  MIG +A      E+L LFD + EEG+ PDKV  + ++NACA  G++  A+ +H  + ++ +  D  LG A++  YAKCG    A  +FD+M  +NVI+W+A+I     HG G+  + +F  M   G+ P+++TFVSLL ACSH+GL+EEG R F  M  +F +   I+HY CMVDLLGRAG+LDE   L+++M  + + R+WGALLGACRIHGN+ +AE+A  S L+L P+NA  YV LS++YA AG W   AK+R LM QR + K PG +WIEV +K + F   DR HP+SEKIYA L+ L   +++ GYVPDT  V+HDVDE  K   +  HSE+LAIA+GLI+T  GTPIRI KNLRVC DCHT TKF++ +  R I+ RDVNRFHHF++G CSCGDYW
Sbjct:  467 EIHSRVVKDGFMFDISVQNALISMYARCGSIKDARLLFNKMVRKDIISWTAMIGGLAKSGLGAEALAVFQDMQQAGLKPNRVTYTSILNACSSPAALDWGRRIHQQVIEAGLATDAHVANTLVNMYSMCGSVKDARQVFDRMTQRDIVAYNAMIGGYAAHNLGKEALKLFDRLQEEGLKPDKVTYINMLNACANSGSLEWAKEIHSLVLKDGYLSDTSLGNALVSTYAKCGSFSDALLVFDKMMKRNVISWNAIIGGCAQHGRGQDVLQLFERMKMEGIKPDIVTFVSLLSACSHAGLLEEGRRYFCSMSRDFGITPTIEHYGCMVDLLGRAGQLDEVEALIKTMPFQANTRIWGALLGACRIHGNVPVAERAAESSLKLDPDNAAVYVALSHMYAAAGMWDSAAKLRKLMEQRGVTKEPGRSWIEVGDKLHYFVAEDRSHPESEKIYAELDKLTHAMKMEGYVPDTRSVMHDVDEGEKENAVCHHSERLAIAYGLISTLPGTPIRIFKNLRVCPDCHTATKFITKIVDREIVARDVNRFHHFKDGVCSCGDYW 1020          

HSP 2 Score: 222.631 bits (566), Expect = 1.770e-55
Identity = 129/401 (32.17%), Postives = 218/401 (54.36%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLE--LQPENAGHYVLLSNIYARAG 1197
            E+H + +   +L ++ VAN +L MYA+   + +A  +F  M  +  V+W++++GG+ + G     F  F+++ + GV P+  T   V+        LK G+ VH  +L  G  +D     ALV MYAKC    D RQ+F+K+ NRDL+ W  MIG  AE  N +E+  ++  M  EG++P+K+  V ++NAC    A+H  R +H  + ++ F  D+ +  A+I MYA+CG I  AR +F++M  K++I+W+AMI      G G +A+A+F  M  +G+ PN +T+ S+L ACS    ++ G RI   +  E  +  D      +V++    G + +A ++ + M  ++D   + A++G    H     A K  + L E  L+P+    Y+ + N  A +G
Sbjct:  265 EIHVEAMKARLLFDVNVANCILNMYAKCGSIHEAREVFDKMETKSVVSWTIIIGGYADCGHSEIAFEIFQKMQQEGVVPNRITYINVLNAFSGPAALKWGKTVHSHILNAGHESDLAVGTALVKMYAKCGSYKDCRQVFEKLVNRDLIAWNTMIGGLAEGGNWEEASEIYHQMQREGMMPNKITYVILLNACVNPTALHWGREIHSRVVKDGFMFDISVQNALISMYARCGSIKDARLLFNKMVRKDIISWTAMIGGLAKSGLGAEALAVFQDMQQAGLKPNRVTYTSILNACSSPAALDWGRRIHQQV-IEAGLATDAHVANTLVNMYSMCGSVKDARQVFDRM-TQRDIVAYNAMIGGYAAHNLGKEALKLFDRLQEEGLKPDKV-TYINMLNACANSG 662          

HSP 3 Score: 214.927 bits (546), Expect = 3.691e-53
Identity = 108/364 (29.67%), Postives = 197/364 (54.12%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            VH+ ++  G   +L V   L+ MYA+     D   +F  +  RD + W+ M+GG    G++      + ++ R G+ P+  T   ++  C +   L  GR +H  V+K G + D     AL+ MYA+C  + DAR LF+KM  +D+++WT MIG  A+     E+L +F  M + G+ P++V   +I+NAC+   A+   R +H  +     + D  +   +++MY+ CG +  AR++FDRM  ++++ ++AMI  Y  H  G++A+ +F  +   G+ P+ +T++++L AC++SG +E    I SL+  +    +D      +V    + G   +AL + + M ++++   W A++G C  HG
Sbjct:  367 VHSHILNAGHESDLAVGTALVKMYAKCGSYKDCRQVFEKLVNRDLIAWNTMIGGLAEGGNWEEASEIYHQMQREGMMPNKITYVILLNACVNPTALHWGREIHSRVVKDGFMFDISVQNALISMYARCGSIKDARLLFNKMVRKDIISWTAMIGGLAKSGLGAEALAVFQDMQQAGLKPNRVTYTSILNACSSPAALDWGRRIHQQVIEAGLATDAHVANTLVNMYSMCGSVKDARQVFDRMTQRDIVAYNAMIGGYAAHNLGKEALKLFDRLQEEGLKPDKVTYINMLNACANSGSLEWAKEIHSLVLKD-GYLSDTSLGNALVSTYAKCGSFSDALLVFDKM-MKRNVISWNAIIGGCAQHG 728          

HSP 4 Score: 198.364 bits (503), Expect = 3.575e-48
Identity = 123/451 (27.27%), Postives = 227/451 (50.33%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMG--ERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYAC-----------SHSGLIEEG--------------------LRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESM---AVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYV-LLSNIYARAGRWKDVAKMRDLMSQR 1242
            EVH  ++ +  + +    N L+ MY Q   + +A  ++  +   ER   +W+ MV G+V  G         RE+ + G+     T   ++  C+    L+ GR +H   +K  LL D      +++MYAKC  + +AR++FDKM+ + +V+WT++IG +A+C +++ +  +F  M +EGVVP+++  + ++NA +   A+   + +H +I       D+ +GTA++ MYAKCG     R++F+++  +++I W+ MI      G+  +A  I+H M   G+ PN IT+V LL AC            HS ++++G                    ++   L++++  VR DI  +T M+  L ++G   EAL + + M    ++ +   + ++L AC     +D   +    ++E       H    L N+Y+  G  KD  ++ D M+QR
Sbjct:  162 EVHEHIIQHCTVLDQYTVNALINMYIQCGSIEEARQVWNKLNHTERTVHSWNAMVVGYVQYGYIEEALKLLREMQQHGLALGRATTMRLLSSCKSPSALECGREIHVEAMKARLLFDVNVANCILNMYAKCGSIHEAREVFDKMETKSVVSWTIIIGGYADCGHSEIAFEIFQKMQQEGVVPNRITYINVLNAFSGPAALKWGKTVHSHILNAGHESDLAVGTALVKMYAKCGSYKDCRQVFEKLVNRDLIAWNTMIGGLAEGGNWEEASEIYHQMQREGMMPNKITYVILLNACVNPTALHWGREIHSRVVKDGFMFDISVQNALISMYARCGSIKDARLLFNKM-VRKDIISWTAMIGGLAKSGLGAEALAVFQDMQQAGLKPNRVTYTSILNACSSPAALDWGRRIHQQVIEAGLATDAHVANTLVNMYSMCGSVKDARQVFDRMTQR 611          

HSP 5 Score: 152.525 bits (384), Expect = 2.250e-34
Identity = 83/310 (26.77%), Postives = 165/310 (53.23%), Query Frame = 1

Query:  259 VIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQN--RDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLE--LQPENAGHYVLLS 1176
            +++ C ++KDL  GR VH+ +++H  + D +   AL++MY +C  +++ARQ+++K+ +  R + +W  M+  + +    +E+L L   M + G+   +   + ++++C    A+   R +H    + +   DV +   +++MYAKCG I  ARE+FD+M+ K+V++W+ +I  Y   GH   A  IF  M   GV PN IT++++L A S    ++ G  + S + +     +D+   T +V +  + G   +  ++ E + V +D   W  ++G     GN + A +  + +    + P    + +LL+
Sbjct:  147 MLKRCIEVKDLVAGREVHEHIIQHCTVLDQYTVNALINMYIQCGSIEEARQVWNKLNHTERTVHSWNAMVVGYVQYGYIEEALKLLREMQQHGLALGRATTMRLLSSCKSPSALECGREIHVEAMKARLLFDVNVANCILNMYAKCGSIHEAREVFDKMETKSVVSWTIIIGGYADCGHSEIAFEIFQKMQQEGVVPNRITYINVLNAFSGPAALKWGKTVHSHILNA-GHESDLAVGTALVKMYAKCGSYKDCRQVFEKL-VNRDLIAWNTMIGGLAEGGNWEEASEIYHQMQREGMMPNKITYVILLN 454          

HSP 6 Score: 70.8626 bits (172), Expect = 8.609e-10
Identity = 45/209 (21.53%), Postives = 104/209 (49.76%), Query Frame = 1

Query:  490 DESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQ--VKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLA 1110
            D ++ +  ++ ++G   +    + ++  C ++  +   R +H +I ++   LD     A+I+MY +CG I+ AR++++++    + V +W+AM+  Y  +G+  +A+ +   M   G++    T + LL +C     +E G R   +   +  +  D+    C++++  + G + EA E+ + M   K    W  ++G     G+ ++A
Sbjct:  123 DRAMDVVQYLQQQGARVNSCDYMKMLKRCIEVKDLVAGREVHEHIIQHCTVLDQYTVNALINMYIQCGSIEEARQVWNKLNHTERTVHSWNAMVVGYVQYGYIEEALKLLREMQQHGLALGRATTMRLLSSCKSPSALECG-REIHVEAMKARLLFDVNVANCILNMYAKCGSIHEAREVFDKMET-KSVVSWTIIIGGYADCGHSEIA 329          

BLAST of ppa025850m vs. ExPASy TrEMBL
Match: A9T8E9_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_192620 PE=4 SV=1)

HSP 1 Score: 528.094 bits (1359), Expect = 1.970e-147
Identity = 260/554 (46.93%), Postives = 359/554 (64.80%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            E+H++V   G   ++ V N L+ MY++   + DA  +F  M  +D ++W+ M+GG    G        ++E+ ++GV P+  T   ++  C     L+ GR +H  V++ GL  D      LV+MY+ C  V DARQ+FD+M  RD+V +  MIG +A      E+L LFD + EEG+ PDKV  + ++NACA  G++  AR +H  + +  F  D  +G A++  YAKCG    A  +F++M  +NVI+W+A+I     HG G+ A+ +F  M   GV P+++TFVSLL ACSH+GL+EEG R F  M  +FA+   I+HY CMVDLLGRAG+LDEA  L+++M  + + R+WGALLGACRIHGN+ +AE+A  S L+L  +NA  YV LS++YA AG W   AK+R LM QR + K PG +WI+V +K + F   DR HPQSEKIYA L+ L   +++ GYVPDT  V+HDVDE  K   +  HSE+LAIA+GLI+T  GT I I KNLRVC DCHT TKF+S +  R II RDVNRFHHF++G CSCGDYW
Sbjct:  349 EIHSRVAKAGFTSDIGVQNALISMYSRCGSIKDARLVFDKMVRKDVISWTAMIGGLAKSGFGAEALTVYQEMQQAGVEPNRVTYTSILNACSSPAALEWGRRIHQQVVEAGLATDAHVGNTLVNMYSMCGSVKDARQVFDRMIQRDIVAYNAMIGGYAAHNLGKEALKLFDRLQEEGLKPDKVTYINMLNACANSGSLEWAREIHTLVRKGGFFSDTSVGNALVSTYAKCGSFSDASIVFEKMTKRNVISWNAIIGGSAQHGRGQDALQLFERMKMEGVKPDIVTFVSLLSACSHAGLLEEGRRYFCSMSQDFAIIPTIEHYGCMVDLLGRAGQLDEAEALIKTMPFQANTRIWGALLGACRIHGNVPVAERAAESSLKLDLDNAVVYVALSHMYAAAGMWDSAAKLRKLMEQRGVTKEPGRSWIQVGDKLHYFVAEDRSHPQSEKIYAELDRLTHAMKMKGYVPDTRSVMHDVDEGEKENAVCHHSERLAIAYGLISTPPGTRIHIFKNLRVCPDCHTATKFISKIVDREIIARDVNRFHHFKDGVCSCGDYW 902          

HSP 2 Score: 228.409 bits (581), Expect = 3.226e-57
Identity = 131/401 (32.67%), Postives = 219/401 (54.61%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLE--LQPENAGHYVLLSNIYARAG 1197
            E+H Q +  G+L ++ VAN +L MYA+   + +A  +F  M ++  V+W++ +GG+ + G     F  F+++ + GV P+  T   V+        LK G+ VH  +L  G  +D     ALV MYAKC    D RQ+F+K+ NRDL+ W  MIG  AE    +E+  +++ M  EGV+P+K+  V ++NAC    A+H  + +H  + +  F+ D+ +  A+I MY++CG I  AR +FD+M  K+VI+W+AMI      G G +A+ ++  M  +GV PN +T+ S+L ACS    +E G RI   +  E  +  D      +V++    G + +A ++ + M +++D   + A++G    H     A K  + L E  L+P+    Y+ + N  A +G
Sbjct:  147 EIHFQAMQAGLLFDVKVANCILNMYAKCGSIEEAREVFDKMEKKSVVSWTITIGGYADCGRSETAFEIFQKMEQEGVVPNRITYISVLNAFSSPAALKWGKAVHSRILNAGHESDTAVGTALVKMYAKCGSYKDCRQVFEKLVNRDLIAWNTMIGGLAEGGYWEEASEVYNQMQREGVMPNKITYVILLNACVNSAALHWGKEIHSRVAKAGFTSDIGVQNALISMYSRCGSIKDARLVFDKMVRKDVISWTAMIGGLAKSGFGAEALTVYQEMQQAGVEPNRVTYTSILNACSSPAALEWGRRIHQQV-VEAGLATDAHVGNTLVNMYSMCGSVKDARQVFDRM-IQRDIVAYNAMIGGYAAHNLGKEALKLFDRLQEEGLKPDKV-TYINMLNACANSG 544          

HSP 3 Score: 204.527 bits (519), Expect = 4.989e-50
Identity = 105/364 (28.85%), Postives = 193/364 (53.02%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            VH++++  G   +  V   L+ MYA+     D   +F  +  RD + W+ M+GG    G +      + ++ R GV P+  T   ++  C +   L  G+ +H  V K G  +D     AL+ MY++C  + DAR +FDKM  +D+++WT MIG  A+     E+L ++  M + GV P++V   +I+NAC+   A+   R +H  +     + D  +G  +++MY+ CG +  AR++FDRM  ++++ ++AMI  Y  H  G++A+ +F  +   G+ P+ +T++++L AC++SG +E    I +L+  +    +D      +V    + G   +A  + E M  +++   W A++G    HG
Sbjct:  249 VHSRILNAGHESDTAVGTALVKMYAKCGSYKDCRQVFEKLVNRDLIAWNTMIGGLAEGGYWEEASEVYNQMQREGVMPNKITYVILLNACVNSAALHWGKEIHSRVAKAGFTSDIGVQNALISMYSRCGSIKDARLVFDKMVRKDVISWTAMIGGLAKSGFGAEALTVYQEMQQAGVEPNRVTYTSILNACSSPAALEWGRRIHQQVVEAGLATDAHVGNTLVNMYSMCGSVKDARQVFDRMIQRDIVAYNAMIGGYAAHNLGKEALKLFDRLQEEGLKPDKVTYINMLNACANSGSLEWAREIHTLV-RKGGFFSDTSVGNALVSTYAKCGSFSDASIVFEKM-TKRNVISWNAIIGGSAQHG 610          

HSP 4 Score: 202.986 bits (515), Expect = 1.452e-49
Identity = 123/451 (27.27%), Postives = 224/451 (49.67%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMG--ERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFS-------------------------------LMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESM---AVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHY-VLLSNIYARAGRWKDVAKMRDLMSQR 1242
            +VH  ++ +  + +    N L+ MY Q   + +A  ++  +   ER   +W+ MV G++  G         R++ + G+ PD  T+   +  C+    L+ GR +H   ++ GLL D      +++MYAKC  +++AR++FDKM+ + +V+WT+ IG +A+C  ++ +  +F  M +EGVVP+++  ++++NA +   A+   + +H  I       D  +GTA++ MYAKCG     R++F+++  +++I W+ MI      G+  +A  +++ M   GV PN IT+V LL AC +S  +  G  I S                               L++D+  VR D+  +T M+  L ++G   EAL + + M    VE +   + ++L AC     ++   +    ++E       H    L N+Y+  G  KD  ++ D M QR
Sbjct:   44 QVHQHIIQHRTVPDQYTVNALINMYIQCGSIEEARQVWKKLSYMERTVHSWNAMVVGYIQYGYIEKALKLLRQMQQHGLAPDRTTIMSFLSSCKSPGALEWGREIHFQAMQAGLLFDVKVANCILNMYAKCGSIEEAREVFDKMEKKSVVSWTITIGGYADCGRSETAFEIFQKMEQEGVVPNRITYISVLNAFSSPAALKWGKAVHSRILNAGHESDTAVGTALVKMYAKCGSYKDCRQVFEKLVNRDLIAWNTMIGGLAEGGYWEEASEVYNQMQREGVMPNKITYVILLNACVNSAALHWGKEIHSRVAKAGFTSDIGVQNALISMYSRCGSIKDARLVFDKM-VRKDVISWTAMIGGLAKSGFGAEALTVYQEMQQAGVEPNRVTYTSILNACSSPAALEWGRRIHQQVVEAGLATDAHVGNTLVNMYSMCGSVKDARQVFDRMIQR 493          

HSP 5 Score: 156.377 bits (394), Expect = 1.558e-35
Identity = 85/310 (27.42%), Postives = 163/310 (52.58%), Query Frame = 1

Query:  259 VIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKM--QNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLE--LQPENAGHYVLLS 1176
            +++ C ++KDL  GR VH  +++H  + D +   AL++MY +C  +++ARQ++ K+    R + +W  M+  + +    +++L L   M + G+ PD+  +++ +++C   GA+   R +H+   +     DV +   +++MYAKCG I+ ARE+FD+M+ K+V++W+  I  Y   G    A  IF  M   GV PN IT++S+L A S    ++ G  + S + +     +D    T +V +  + G   +  ++ E + V +D   W  ++G     G  + A +  N +    + P    + +LL+
Sbjct:   29 MLKRCIEVKDLVAGRQVHQHIIQHRTVPDQYTVNALINMYIQCGSIEEARQVWKKLSYMERTVHSWNAMVVGYIQYGYIEKALKLLRQMQQHGLAPDRTTIMSFLSSCKSPGALEWGREIHFQAMQAGLLFDVKVANCILNMYAKCGSIEEAREVFDKMEKKSVVSWTITIGGYADCGRSETAFEIFQKMEQEGVVPNRITYISVLNAFSSPAALKWGKAVHSRILNA-GHESDTAVGTALVKMYAKCGSYKDCRQVFEKL-VNRDLIAWNTMIGGLAEGGYWEEASEVYNQMQREGVMPNKITYVILLN 336          

HSP 6 Score: 82.0333 bits (201), Expect = 3.734e-13
Identity = 48/199 (24.12%), Postives = 104/199 (52.26%), Query Frame = 1

Query:  490 DESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQV--KNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRI-FSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLG 1077
            D ++ +  ++ ++G   +    + ++  C ++  +   R +H +I +++   D     A+I+MY +CG I+ AR+++ ++    + V +W+AM+  Y  +G+  KA+ +   M   G++P+  T +S L +C   G +E G  I F  M  +  +  D+K   C++++  + G ++EA E+ + M  +K    W   +G
Sbjct:    5 DGAVDVVQYLQQQGAQVNSSDYMKMLKRCIEVKDLVAGRQVHQHIIQHRTVPDQYTVNALINMYIQCGSIEEARQVWKKLSYMERTVHSWNAMVVGYIQYGYIEKALKLLRQMQQHGLAPDRTTIMSFLSSCKSPGALEWGREIHFQAM--QAGLLFDVKVANCILNMYAKCGSIEEAREVFDKME-KKSVVSWTITIG 200          

BLAST of ppa025850m vs. ExPASy TrEMBL
Match: B8LQA8_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 520.39 bits (1339), Expect = 4.107e-145
Identity = 247/554 (44.58%), Postives = 369/554 (66.61%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            ++H   + +G+  ++ V N L+ MYA+   +  A+ LF  M  RD  +W+ ++GG+          A F  +   G+ P+  T+  V+  C  +  L+ G+ +H   ++ G  +++    ALV+MYAKC  V+ A +LF++M  +++V W  +I  +++  +  E+L LF  M  +G+ PD  A+V+++ ACA   A+ + + +H Y  R+ F  +V++GT ++D+YAKCG +++A+++F+RM  ++V++W+ MI AYG HGHG  A+A+F  M  +G   + I F ++L ACSH+GL+++GL+ F  M  ++ +   ++HY C+VDLLGRAG LDEA  ++++M++E D  +WGALLGACRIH NI+L E+A   L EL P+NAG+YVLLSNIYA A RW+DVAK+R +M ++ +KK PG + + V      F  GDR HPQSE+IYAMLE L +++  AGYVP+TN  L DV+EE K  +L SHSEKLAI+FG+I T+ G PIRI KNLRVC DCH  TKF+S +  R IIVRD NRFHH + G CSCGDYW
Sbjct:  242 QIHCYAIRSGIESDVLVVNGLVNMYAKCGNVNTAHKLFERMPIRDVASWNAIIGGYSLNSQHHEALAFFNRMQVRGIKPNSITMVSVLPACAHLFALEQGQQIHGYAIRSGFESNDVVGNALVNMYAKCGNVNSAYKLFERMPKKNVVAWNAIISGYSQHGHPHEALALFIEMQAQGIKPDSFAIVSVLPACAHFLALEQGKQIHGYTIRSGFESNVVVGTGLVDIYAKCGNVNTAQKLFERMPEQDVVSWTTMILAYGIHGHGEDALALFSKMQETGTKLDHIAFTAILTACSHAGLVDQGLQYFQCMKSDYGLAPKLEHYACLVDLLGRAGHLDEANGIIKNMSLEPDANVWGALLGACRIHCNIELGEQAAKHLFELDPDNAGYYVLLSNIYAEAQRWEDVAKLRKMMKEKGVKKQPGCSVVAVHRDVQTFLVGDRTHPQSEQIYAMLEILYEQMRKAGYVPNTNLALQDVEEEAKENILSSHSEKLAISFGIINTSPGIPIRIMKNLRVCSDCHNATKFISKIVGREIIVRDANRFHHVKNGFCSCGDYW 795          

HSP 2 Score: 224.557 bits (571), Expect = 4.659e-56
Identity = 120/370 (32.43%), Postives = 209/370 (56.49%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHY----TCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGN 1098
            +VH  ++  G   ++ V   L  MY +   L +A  +F  M +RD V+W+ ++ G+   G      A F E+  +G+ P+  TL  V+ VC  +  L+ G+ +H   ++ G+ +D      LV+MYAKC  V+ A +LF++M  RD+ +W  +IG ++      E+L  F+ M   G+ P+ + MV+++ ACA L A+ + + +H Y  R+ F  + ++G A+++MYAKCG ++SA ++F+RM  KNV+ W+A+I+ Y  HGH  +A+A+F  M   G+ P+    VS+L AC+H   +E+G +I       + +R+  +      T +VD+  + G ++ A +L E M  E+D   W  ++ A  IHG+
Sbjct:  141 KVHEDIIARGFESDVIVGTALASMYTKCGSLENARQVFDRMPKRDVVSWNAIIAGYSQNGQPYEALALFSEMQVNGIKPNSSTLVSVMPVCAHLLALEQGKQIHCYAIRSGIESDVLVVNGLVNMYAKCGNVNTAHKLFERMPIRDVASWNAIIGGYSLNSQHHEALAFFNRMQVRGIKPNSITMVSVLPACAHLFALEQGQQIHGYAIRSGFESNDVVGNALVNMYAKCGNVNSAYKLFERMPKKNVVAWNAIISGYSQHGHPHEALALFIEMQAQGIKPDSFAIVSVLPACAHFLALEQGKQIHG-----YTIRSGFESNVVVGTGLVDIYAKCGNVNTAQKLFERMP-EQDVVSWTTMILAYGIHGH 504          

HSP 3 Score: 199.519 bits (506), Expect = 1.605e-48
Identity = 103/343 (30.03%), Postives = 190/343 (55.39%), Query Frame = 1

Query:  133 DSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHY----TCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPE 1149
            ++V W   + G+V  G +      + ++ R+G+ PD      VI+ C    DL+ GR VH+ ++  G  +D     AL  MY KC  +++ARQ+FD+M  RD+V+W  +I  +++     E+L LF  M   G+ P+   +V+++  CA L A+ + + +H Y  R+    DV++   +++MYAKCG +++A ++F+RM +++V +W+A+I  Y  +    +A+A F+ M   G+ PN IT VS+L AC+H   +E+G +I       +A+R+  +        +V++  + G ++ A +L E M  +K+   W A++     HG+     +A+   +E+Q +
Sbjct:   84 NAVVWKETIIGYVKNGFWNKALRLYYQMQRTGINPDKLVFLSVIKACGSQSDLQAGRKVHEDIIARGFESDVIVGTALASMYTKCGSLENARQVFDRMPKRDVVSWNAIIAGYSQNGQPYEALALFSEMQVNGIKPNSSTLVSVMPVCAHLLALEQGKQIHCYAIRSGIESDVLVVNGLVNMYAKCGNVNTAHKLFERMPIRDVASWNAIIGGYSLNSQHHEALAFFNRMQVRGIKPNSITMVSVLPACAHLFALEQGQQIHG-----YAIRSGFESNDVVGNALVNMYAKCGNVNSAYKLFERMP-KKNVVAWNAIISGYSQHGH---PHEALALFIEMQAQ 417          

BLAST of ppa025850m vs. ExPASy Swiss-Prot
Match: PP348_ARATH (Pentatricopeptide repeat-containing protein At4g33990 OS=Arabidopsis thaliana GN=EMB2758 PE=2 SV=2)

HSP 1 Score: 481.871 bits (1239), Expect = 5.231e-135
Identity = 236/555 (42.52%), Postives = 362/555 (65.23%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACA-ALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEG-VVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +H+  + +G+   L V+NKL+ +YA+   L D   +F  M  RD ++W+ ++  +          + F+E+  S + PD  TL  +  +   + D++  R V    L+ G   ++     A+V MYAK  +VD AR +F+ + N D+++W  +I  +A+   A E++ +++ M EEG +  ++   V+++ AC++ GA+ +   LH  + +N   LDV + T++ DMY KCG ++ A  +F ++   N + W+ +IA +G+HGHG KA+ +F  ML+ GV P+ ITFV+LL ACSHSGL++EG   F +M  ++ +   +KHY CMVD+ GRAG+L+ AL+ ++SM+++ D  +WGALL ACR+HGN+DL + A   L E++PE+ G++VLLSN+YA AG+W+ V ++R +   + L+K PG + +EVDNK   F TG++ HP  E++Y  L +L  KL++ GYVPD  FVL DV+++ K  +L SHSE+LAIAF LIAT   T IRI KNLRVCGDCH+ TKF+S +T+R IIVRD NRFHHF+ G CSCGDYW
Sbjct:  269 IHSYSIKHGLESELFVSNKLIDLYAEFGRLRDCQKVFDRMYVRDLISWNSIIKAYELNEQPLRAISLFQEMRLSRIQPDCLTLISLASILSQLGDIRACRSVQGFTLRKGWFLEDITIGNAVVVMYAKLGLVDSARAVFNWLPNTDVISWNTIISGYAQNGFASEAIEMYNIMEEEGEIAANQGTWVSVLPACSQAGALRQGMKLHGRLLKNGLYLDVFVVTSLADMYGKCGRLEDALSLFYQIPRVNSVPWNTLIACHGFHGHGEKAVMLFKEMLDEGVKPDHITFVTLLSACSHSGLVDEGQWCFEMMQTDYGITPSLKHYGCMVDMYGRAGQLETALKFIKSMSLQPDASIWGALLSACRVHGNVDLGKIASEHLFEVEPEHVGYHVLLSNMYASAGKWEGVDEIRSIAHGKGLRKTPGWSSMEVDNKVEVFYTGNQTHPMYEEMYRELTALQAKLKMIGYVPDHRFVLQDVEDDEKEHILMSHSERLAIAFALIATPAKTTIRIFKNLRVCGDCHSVTKFISKITEREIIVRDSNRFHHFKNGVCSCGDYW 823          

HSP 2 Score: 172.17 bits (435), Expect = 8.866e-42
Identity = 112/410 (27.32%), Postives = 209/410 (50.98%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRE-LIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPE---NAGHYVLLSNIYARAGRWKDVAKM 1221
            +HA++VV+  +QN+ ++ KL+ +Y     +  A   F  +  RD   W++M+ G+   G+ +     F   ++ SG+TPD  T P V++ CR + D   G  +H + LK G + D +  A+L+ +Y++ K V +AR LFD+M  RD+ +W  MI  + +  NA E+L L + +       D V +V++++AC + G  +R   +H Y  ++    ++ +   +ID+YA+ G +   +++FDRM V+++I+W+++I AY  +    +AI++F  M  S + P+ +T +SL    S  G I     +      +     DI     +V +  + G +D A   V +     D   W  ++     +G    A +  N ++E + E   N G +V +    ++AG  +   K+
Sbjct:   73 LHARLVVSKQIQNVCISAKLVNLYCYLGNVALARHTFDHIQNRDVYAWNLMISGYGRAGNSSEVIRCFSLFMLSSGLTPDYRTFPSVLKACRTVID---GNKIHCLALKFGFMWDVYVAASLIHLYSRYKAVGNARILFDEMPVRDMGSWNAMISGYCQSGNAKEALTLSNGLR----AMDSVTVVSLLSACTEAGDFNRGVTIHSYSIKHGLESELFVSNKLIDLYAEFGRLRDCQKVFDRMYVRDLISWNSIIKAYELNEQPLRAISLFQEMRLSRIQPDCLTLISLASILSQLGDIRACRSVQGFTLRKGWFLEDITIGNAVVVMYAKLGLVDSA-RAVFNWLPNTDVISWNTIISGYAQNGFASEAIEMYN-IMEEEGEIAANQGTWVSVLPACSQAGALRQGMKL 473          

HSP 3 Score: 69.707 bits (169), Expect = 6.197e-11
Identity = 55/198 (27.78%), Postives = 92/198 (46.46%), Query Frame = 1

Query:  559 TIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHL-MLNSGVSPNVITFVSLLYACSHSGLIEEGLRI------FSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSL 1131
            T+   C  L +   A+ LH  +  ++   +V +   ++++Y   G +  AR  FD +Q ++V  W+ MI+ YG  G+  + I  F L ML+SG++P+  TF S+L AC     + +G +I      F  MWD +   +       ++ L  R   +  A  L + M V +D   W A++      GN   A    N L
Sbjct:   59 TLFRYCTNLQS---AKCLHARLVVSKQIQNVCISAKLVNLYCYLGNVALARHTFDHIQNRDVYAWNLMISGYGRAGNSSEVIRCFSLFMLSSGLTPDYRTFPSVLKACR---TVIDGNKIHCLALKFGFMWDVYVAAS-------LIHLYSRYKAVGNARILFDEMPV-RDMGSWNAMISGYCQSGNAKEALTLSNGL 242          

BLAST of ppa025850m vs. ExPASy Swiss-Prot
Match: PPR32_ARATH (Pentatricopeptide repeat-containing protein At1g11290 OS=Arabidopsis thaliana GN=PCMP-H40 PE=2 SV=1)

HSP 1 Score: 477.248 bits (1227), Expect = 1.288e-133
Identity = 238/554 (42.96%), Postives = 348/554 (62.82%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            E+H   + +G    + ++  L+ MYA+   L  A  LF GM ER+ V+W+ M+  +V   +       F++++  GV P   ++   +  C D+ DL+ GR +H + ++ GL  +     +L+ MY KCK VD A  +F K+Q+R LV+W  MI  FA+     ++L  F  M    V PD    V+++ A A+L   H A+ +H  + R+    +V + TA++DMYAKCG I  AR IFD M  ++V TW+AMI  YG HG G+ A+ +F  M    + PN +TF+S++ ACSHSGL+E GL+ F +M + +++   + HY  MVDLLGRAGRL+EA + +  M V+    ++GA+LGAC+IH N++ AEKA   L EL P++ G++VLL+NIY  A  W+ V ++R  M ++ L+K PG + +E+ N+ + F +G   HP S+KIYA LE L   ++ AGYVPDTN VL  V+ +VK  +L +HSEKLAI+FGL+ T  GT I + KNLRVC DCH  TK++S VT R I+VRD+ RFHHF+ G CSCGDYW
Sbjct:  257 EIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEVDTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITAIAELSITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMSERHVTTWNAMIDGYGTHGFGKAALELFEEMQKGTIKPNGVTFLSVISACSHSGLVEAGLKCFYMMKENYSIELSMDHYGAMVDLLGRAGRLNEAWDFIMQMPVKPAVNVYGAMLGACQIHKNVNFAEKAAERLFELNPDDGGYHVLLANIYRAASMWEKVGQVRVSMLRQGLRKTPGCSMVEIKNEVHSFFSGSTAHPDSKKIYAFLEKLICHIKEAGYVPDTNLVL-GVENDVKEQLLSTHSEKLAISFGLLNTTAGTTIHVRKNLRVCADCHNATKYISLVTGREIVVRDMQRFHHFKNGACSCGDYW 809          

HSP 2 Score: 191.815 bits (486), Expect = 1.081e-47
Identity = 97/303 (32.01%), Postives = 175/303 (57.76%), Query Frame = 1

Query:   16 VVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSL 924
            V  NG+ Q      KL+ ++ +   + +A  +F  +  + +V +  M+ GF  V D       F  +    V P +Y   ++++VC D  +L++G+ +H +++K G   D FA   L +MYAKC+ V++AR++FD+M  RDLV+W  ++  +++   A  +L +   M EE + P  + +V+++ A + L  +   + +H Y  R+ F   V + TA++DMYAKCG +++AR++FD M  +NV++W++MI AY  + + ++A+ IF  ML+ GV P  ++ +  L+AC+  G +E G  I  L
Sbjct:   60 VFKNGLYQEHFFQTKLVSLFCRYGSVDEAARVFEPIDSKLNVLYHTMLKGFAKVSDLDKALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVGKEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKPTDVSVMGALHACADLGDLERGRFIHKL 362          

HSP 3 Score: 179.874 bits (455), Expect = 4.252e-44
Identity = 103/365 (28.22%), Postives = 186/365 (50.96%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            E+H  +V +G   +L     L  MYA+ R + +A  +F  M ERD V+W+ +V G+   G         + +    + P   T+  V+     ++ + +G+ +H   ++ G  +      ALVDMYAKC  ++ ARQLFD M  R++V+W  MI A+ +  N  E++++F  M++EGV P  V+++  ++ACA LG + R R +H          +V +  ++I MY KC  +D+A  +F ++Q + +++W+AMI  +  +G    A+  F  M +  V P+  T+VS++ A +   +      I  ++     +  ++   T +VD+  + G +  A  L+  M  E+    W A++     HG
Sbjct:  156 EIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEVDTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITAIAELSITHHAKWIHGVVM-RSCLDKNVFVTTALVDMYAKCGAIMIA-RLIFDMMSERHVTTWNAMIDGYGTHG 518          

HSP 4 Score: 125.176 bits (313), Expect = 1.243e-27
Identity = 78/293 (26.62%), Postives = 153/293 (52.22%), Query Frame = 1

Query:  220 RSGVTPDIYTLP--FVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRAD----IKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGA 1080
            R+ +  ++Y  P   ++  C  +K+L   R +  +V K+GL  ++F    LV ++ +   VD+A ++F+ + ++  V +  M+  FA+  + D++L  F  M  + V P       ++  C     +   + +H  + ++ FSLD+   T + +MYAKC  ++ AR++FDRM  +++++W+ ++A Y  +G  R A+ +   M    + P+ IT VS+L A S   LI  G  I       +A+R+     +   T +VD+  + G L+ A +L + M +E++   W +++ A
Sbjct:   28 RNYIPANVYEHPAALLLERCSSLKEL---RQILPLVFKNGLYQEHFFQTKLVSLFCRYGSVDEAARVFEPIDSKLNVLYHTMLKGFAKVSDLDKALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVGKEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHG-----YAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGM-LERNVVSWNSMIDA 311          

BLAST of ppa025850m vs. ExPASy Swiss-Prot
Match: PP265_ARATH (Pentatricopeptide repeat-containing protein At3g46790, chloroplastic OS=Arabidopsis thaliana GN=CRR2 PE=2 SV=1)

HSP 1 Score: 474.167 bits (1219), Expect = 1.091e-132
Identity = 235/559 (42.04%), Postives = 353/559 (63.15%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCR----DMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEE--GVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            VH  ++ NG  Q+  +A KL+ MY+    +  A  +F    +R    W+ +       G        + ++ R GV  D +T  +V++ C      +  L  G+ +H  + + G  +  +    LVDMYA+   VD A  +F  M  R++V+W+ MI  +A+   A E+L  F  M+ E     P+ V MV+++ ACA L A+ + +L+H YI R      + + +A++ MY +CG ++  + +FDRM  ++V++W+++I++YG HG+G+KAI IF  ML +G SP  +TFVS+L ACSH GL+EEG R+F  MW +  ++  I+HY CMVDLLGRA RLDEA ++V+ M  E   ++WG+LLG+CRIHGN++LAE+A   L  L+P+NAG+YVLL++IYA A  W +V +++ L+  R L+K+PG  W+EV  K Y F + D  +P  E+I+A L  L + ++  GY+P T  VL++++ E K  ++  HSEKLA+AFGLI T+ G PIRITKNLR+C DCH FTKF+S   ++ I+VRDVNRFH F+ G CSCGDYW
Sbjct:   99 VHRHILDNGSDQDPFLATKLIGMYSDLGSVDYARKVFDKTRKRTIYVWNALFRALTLAGHGEEVLGLYWKMNRIGVESDRFTYTYVLKACVASECTVNHLMKGKEIHAHLTRRGYSSHVYIMTTLVDMYARFGCVDYASYVFGGMPVRNVVSWSAMIACYAKNGKAFEALRTFREMMRETKDSSPNSVTMVSVLQACASLAALEQGKLIHGYILRRGLDSILPVISALVTMYGRCGKLEVGQRVFDRMHDRDVVSWNSLISSYGVHGYGKKAIQIFEEMLANGASPTPVTFVSVLGACSHEGLVEEGKRLFETMWRDHGIKPQIEHYACMVDLLGRANRLDEAAKMVQDMRTEPGPKVWGSLLGSCRIHGNVELAERASRRLFALEPKNAGNYVLLADIYAEAQMWDEVKRVKKLLEHRGLQKLPGRCWMEVRRKMYSFVSVDEFNPLMEQIHAFLVKLAEDMKEKGYIPQTKGVLYELETEEKERIVLGHSEKLALAFGLINTSKGEPIRITKNLRLCEDCHLFTKFISKFMEKEILVRDVNRFHRFKNGVCSCGDYW 657          

HSP 2 Score: 139.428 bits (350), Expect = 6.368e-32
Identity = 89/314 (28.34%), Postives = 152/314 (48.41%), Query Frame = 1

Query:  232 TPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNAC----AKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLML--NSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENA 1155
            +P   T   +I  C     L     VH  +L +G   D F    L+ MY+    VD AR++FDK + R +  W  +  A     + +E L L+  M   GV  D+     ++ AC      +  + + + +H ++ R  +S  V + T ++DMYA+ GC+D A  +F  M V+NV++WSAMIA Y  +G   +A+  F  M+      SPN +T VS+L AC+    +E+G  I   +     + + +   + +V + GR G+L+    + + M  ++D   W +L+ +  +HG     +KA+    E+    A
Sbjct:   74 SPSQQTYELLILCCGHRSSLSDALRVHRHILDNGSDQDPFLATKLIGMYSDLGSVDYARKVFDKTRKRTIYVWNALFRALTLAGHGEEVLGLYWKMNRIGVESDRFTYTYVLKACVASECTVNHLMKGKEIHAHLTRRGYSSHVYIMTTLVDMYARFGCVDYASYVFGGMPVRNVVSWSAMIACYAKNGKAFEALRTFREMMRETKDSSPNSVTMVSVLQACASLAALEQGKLIHGYILRR-GLDSILPVISALVTMYGRCGKLEVGQRVFDRMH-DRDVVSWNSLISSYGVHG---YGKKAIQIFEEMLANGA 382          

HSP 3 Score: 122.094 bits (305), Expect = 1.052e-26
Identity = 68/267 (25.47%), Postives = 137/267 (51.31%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIR--SGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQ-FSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVK-NVITWSAMIAAYGYHGH 789
            E+HA +   G   ++ +   L+ MYA+   +  A  +FGGM  R+ V+WS M+  +   G       TFRE++R     +P+  T+  V++ C  +  L+ G+L+H  +L+ GL +     +ALV MY +C  ++  +++FD+M +RD+V+W  +I ++       +++ +F+ M+  G  P  V  V+++ AC+  G +   + L   + R+      +     M+D+  +   +D A ++   M+ +     W +++ +   HG+
Sbjct:  203 EIHAHLTRRGYSSHVYIMTTLVDMYARFGCVDYASYVFGGMPVRNVVSWSAMIACYAKNGKAFEALRTFREMMRETKDSSPNSVTMVSVLQACASLAALEQGKLIHGYILRRGLDSILPVISALVTMYGRCGKLEVGQRVFDRMHDRDVVSWNSLISSYGVHGYGKKAIQIFEEMLANGASPTPVTFVSVLGACSHEGLVEEGKRLFETMWRDHGIKPQIEHYACMVDLLGRANRLDEAAKMVQDMRTEPGPKVWGSLLGSCRIHGN 469          

BLAST of ppa025850m vs. ExPASy Swiss-Prot
Match: PP285_ARATH (Pentatricopeptide repeat-containing protein At3g57430, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H81 PE=2 SV=2)

HSP 1 Score: 472.626 bits (1215), Expect = 3.174e-132
Identity = 236/568 (41.55%), Postives = 357/568 (62.85%), Query Frame = 1

Query:    1 EVHAQVVVNGML-QNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRS-GVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWM------IEEG-----VVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDER-LWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            E+HA  + NG L +N  V + L+ MY   + +     +F GM +R    W+ M+ G+            F  +  S G+  +  T+  V+  C           +H  V+K GL  D F    L+DMY++   +D A ++F KM++RDLVTW  MI  +    + +++L+L   M      + +G     + P+ + ++TI+ +CA L A+ + + +H Y  +N  + DV +G+A++DMYAKCGC+  +R++FD++  KNVITW+ +I AYG HG+G++AI +  +M+  GV PN +TF+S+  ACSHSG+++EGLRIF +M  ++ V     HY C+VDLLGRAGR+ EA +L+  M  + ++   W +LLGA RIH N+++ E A  +L++L+P  A HYVLL+NIY+ AG W    ++R  M ++ ++K PG +WIE  ++ ++F  GD  HPQSEK+   LE+L +++   GYVPDT+ VLH+V+E+ K  +L  HSEKLAIAFG++ T+ GT IR+ KNLRVC DCH  TKF+S +  R II+RDV RFH F+ GTCSCGDYW
Sbjct:  323 ELHAYALKNGSLDENSFVGSALVDMYCNCKQVLSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTMAGVVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMEDRDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRVSLKPNSITLMTILPSCAALSALAKGKEIHAYAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVFDQIPQKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHSGMVDEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEAYQLMNMMPRDFNKAGAWSSLLGASRIHNNLEIGEIAAQNLIQLEPNVASHYVLLANIYSSAGLWDKATEVRRNMKEQGVRKEPGCSWIEHGDEVHKFVAGDSSHPQSEKLSGYLETLWERMRKEGYVPDTSCVLHNVEEDEKEILLCGHSEKLAIAFGILNTSPGTIIRVAKNLRVCNDCHLATKFISKIVDREIILRDVRRFHRFKNGTCSCGDYW 890          

HSP 2 Score: 182.956 bits (463), Expect = 5.023e-45
Identity = 122/465 (26.24%), Postives = 221/465 (47.53%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADN-FACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEE-GVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLN-----------SGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVR----ADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGN----IDL----------------------------AEKAVNSLLELQPE-----NAGHYVLLSNIYARAGRWKDVAKMRDLM 1233
            +VHA  +  G L N  + N L+ MY +   L  +  L G  G RD VTW+ ++                RE++  GV PD +T+  V+  C  ++ L+ G+ +H   LK+G L +N F  +ALVDMY  CK V   R++FD M +R +  W  MI  +++  +  E+L+LF  M E  G++ +   M  +V AC + GA  R   +H ++ +     D  +   ++DMY++ G ID A  IF +M+ ++++TW+ MI  Y +  H   A+ + H M N             + PN IT +++L +C+    + +G  I +     +A++     D+   + +VD+  + G L  + ++ + +  +K+   W  ++ A  +HGN    IDL                             ++ +     ++P+     ++ HY  + ++  RAGR K+  ++ ++M
Sbjct:  223 QVHAYGLRKGEL-NSFIINTLVAMYGKLGKLASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLEALEYLREMVLEGVEPDEFTISSVLPACSHLEMLRTGKELHAYALKNGSLDENSFVGSALVDMYCNCKQVLSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTMAGVVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMEDRDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRVSLKPNSITLMTILPSCAALSALAKGKEIHA-----YAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVFDQIP-QKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHSGMVDEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEAYQLMNMM 680          

HSP 3 Score: 153.68 bits (387), Expect = 3.263e-36
Identity = 105/365 (28.77%), Postives = 178/365 (48.77%), Query Frame = 1

Query:    1 EVHAQVVVNGM-LQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDM---KDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLD--VILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNS-GVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALL 1074
            ++HA V   G  + ++TVAN L+ +Y +    G  Y +F  + ER+ V+W+ ++    +   +      FR ++   V P  +TL  V+  C ++   + L MG+ VH   L+ G L ++F    LV MY K   +  ++ L      RDLVTW  ++ +  +     E+L     M+ EGV PD+  + +++ AC+ L  +   + LH Y  +N  SLD    +G+A++DMY  C  + S R +FD M  + +  W+AMIA Y  + H ++A+ +F  M  S G+  N  T   ++ AC  SG       I   +      R      T M D+  R G++D A+ +   M  ++D   W  ++
Sbjct:  118 QIHAHVYKFGYGVDSVTVANTLVNLYRKCGDFGAVYKVFDRISERNQVSWNSLISSLCSFEKWEMALEAFRCMLDENVEPSSFTLVSVVTACSNLPMPEGLMMGKQVHAYGLRKGEL-NSFIINTLVAMYGKLGKLASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLEALEYLREMVLEGVEPDEFTISSVLPACSHLEMLRTGKELHAYALKNG-SLDENSFVGSALVDMYCNCKQVLSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTMAGVVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTLM-DMYSRLGKIDIAMRIFGKME-DRDLVTWNTMI 478          

HSP 4 Score: 111.694 bits (278), Expect = 1.422e-23
Identity = 69/295 (23.39%), Postives = 145/295 (49.15%), Query Frame = 1

Query:  202 TFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACA-ALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKL---GAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALL 1074
            T+ ++I  G+ PD Y  P +++   D++D+++G+ +H  V K G   D+   A  LV++Y KC       ++FD++  R+ V+W  +I +       + +L  F  M++E V P    +V++V AC+ L     +   + +H Y  R       I+ T ++ MY K G + S++ +      ++++TW+ ++++   +    +A+     M+  GV P+  T  S+L ACSH  ++  G  + +      ++  +    + +VD+     ++     + + M  ++   LW A++
Sbjct:   84 TYVDMIVLGIKPDNYAFPALLKAVADLQDMELGKQIHAHVYKFGYGVDSVTVANTLVNLYRKCGDFGAVYKVFDRISERNQVSWNSLISSLCSFEKWEMALEAFRCMLDENVEPSSFTLVSVVTACSNLPMPEGLMMGKQVHAYGLRKGELNSFIINT-LVAMYGKLGKLASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLEALEYLREMVLEGVEPDEFTISSVLPACSHLEMLRTGKELHAYALKNGSLDENSFVGSALVDMYCNCKQVLSGRRVFDGM-FDRKIGLWNAMI 376          

HSP 5 Score: 77.0258 bits (188), Expect = 3.882e-13
Identity = 51/221 (23.08%), Postives = 108/221 (48.87%), Query Frame = 1

Query:  427 QNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLD-VILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHY--TCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGA 1080
            Q+R    W  ++ +        E+++ +  MI  G+ PD  A   ++ A A L  M   + +H ++ +  + +D V +   ++++Y KCG   +  ++FDR+  +N ++W+++I++         A+  F  ML+  V P+  T VS++ ACS+  +  EGL +   +      + ++  +    +V + G+ G+L  +  L+ S    +D   W  +L +
Sbjct:   58 QSRSPEWWIDLLRSKVRSNLLREAVLTYVDMIVLGIKPDNYAFPALLKAVADLQDMELGKQIHAHVYKFGYGVDSVTVANTLVNLYRKCGDFGAVYKVFDRISERNQVSWNSLISSLCSFEKWEMALEAFRCMLDENVEPSSFTLVSVVTACSNLPM-PEGLMMGKQVHAYGLRKGELNSFIINTLVAMYGKLGKLASSKVLLGSFG-GRDLVTWNTVLSS 276          

BLAST of ppa025850m vs. ExPASy Swiss-Prot
Match: PP224_ARATH (Pentatricopeptide repeat-containing protein At3g12770 OS=Arabidopsis thaliana GN=PCMP-H43 PE=2 SV=1)

HSP 1 Score: 468.774 bits (1205), Expect = 4.583e-131
Identity = 233/555 (41.98%), Postives = 349/555 (62.88%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMG--ERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            VHAQV   G   ++ V N L+ +YA+ R LG A  +F G+   ER  V+W+ +V  +   G+       F ++ +  V PD   L  V+     ++DLK GR +H  V+K GL  +     +L  MYAKC  V  A+ LFDKM++ +L+ W  MI  +A+   A E++ +F  MI + V PD +++ + ++ACA++G++ +AR ++ Y+ R+ +  DV + +A+IDM+AKCG ++ AR +FDR   ++V+ WSAMI  YG HG  R+AI+++  M   GV PN +TF+ LL AC+HSG++ EG   F+ M D   +    +HY C++DLLGRAG LD+A E+++ M V+    +WGALL AC+ H +++L E A   L  + P N GHYV LSN+YA A  W  VA++R  M ++ L K  G +W+EV  +   F  GD+ HP+ E+I   +E +  +L+  G+V + +  LHD+++E     L SHSE++AIA+GLI+T  GTP+RITKNLR C +CH  TK +S +  R I+VRD NRFHHF++G CSCGDYW
Sbjct:  141 VHAQVFRLGFDADVFVQNGLIALYAKCRRLGSARTVFEGLPLPERTIVSWTAIVSAYAQNGEPMEALEIFSQMRKMDVKPDWVALVSVLNAFTCLQDLKQGRSIHASVVKMGLEIEPDLLISLNTMYAKCGQVATAKILFDKMKSPNLILWNAMISGYAKNGYAREAIDMFHEMINKDVRPDTISITSAISACAQVGSLEQARSMYEYVGRSDYRDDVFISSALIDMFAKCGSVEGARLVFDRTLDRDVVVWSAMIVGYGLHGRAREAISLYRAMERGGVHPNDVTFLGLLMACNHSGMVREGWWFFNRMADH-KINPQQQHYACVIDLLGRAGHLDQAYEVIKCMPVQPGVTVWGALLSACKKHRHVELGEYAAQQLFSIDPSNTGHYVQLSNLYAAARLWDRVAEVRVRMKEKGLNKDVGCSWVEVRGRLEAFRVGDKSHPRYEEIERQVEWIESRLKEGGFVANKDASLHDLNDEEAEETLCSHSERIAIAYGLISTPQGTPLRITKNLRACVNCHAATKLISKLVDREIVVRDTNRFHHFKDGVCSCGDYW 694          

HSP 2 Score: 188.348 bits (477), Expect = 1.195e-46
Identity = 103/367 (28.07%), Postives = 199/367 (54.22%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFD--KMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            ++HA+++V G+  +  +  KL++  +    +  A  +F  +       W+ ++ G+     F +    +  +  + V+PD +T P +++ C  +  L+MGR VH  V + G  AD F    L+ +YAKC+ +  AR +F+   +  R +V+WT ++ A+A+     E+L +F  M +  V PD VA+V+++NA   L  + + R +H  + +    ++  L  ++  MYAKCG + +A+ +FD+M+  N+I W+AMI+ Y  +G+ R+AI +FH M+N  V P+ I+  S + AC+  G +E+   ++  +      R D+   + ++D+  + G + E   LV    +++D  +W A++    +HG
Sbjct:   39 QIHARLLVLGLQFSGFLITKLIHASSSFGDITFARQVFDDLPRPQIFPWNAIIRGYSRNNHFQDALLMYSNMQLARVSPDSFTFPHLLKACSGLSHLQMGRFVHAQVFRLGFDADVFVQNGLIALYAKCRRLGSARTVFEGLPLPERTIVSWTAIVSAYAQNGEPMEALEIFSQMRKMDVKPDWVALVSVLNAFTCLQDLKQGRSIHASVVKMGLEIEPDLLISLNTMYAKCGQVATAKILFDKMKSPNLILWNAMISGYAKNGYAREAIDMFHEMINKDVRPDTISITSAISACAQVGSLEQARSMYEYV-GRSDYRDDVFISSALIDMFAKCGSV-EGARLVFDRTLDRDVVVWSAMIVGYGLHG 403          

BLAST of ppa025850m vs. ExPASy Swiss-Prot
Match: PP390_ARATH (Pentatricopeptide repeat-containing protein At5g16860 OS=Arabidopsis thaliana GN=PCMP-H92 PE=2 SV=1)

HSP 1 Score: 465.307 bits (1196), Expect = 5.067e-130
Identity = 241/601 (40.10%), Postives = 352/601 (58.57%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATF-----------------------------------RELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKH-------GLLADNFACAALVDMYAKCKVVDDARQLFDKM--QNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEG--VVPDKVAMVTIVNACAKLGAMHRARLLHYYICRN-QFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            ++H   V + M+QN+ V N L+ MYA+  ++ +A  +F  M  +D V+W+ MV G+  +G F +    F                                   R+++ SG+ P+  TL  V+  C  +  L  G+ +H   +K+       G   +N     L+DMYAKCK VD AR +FD +  + RD+VTWTVMIG +++  +A+++L L   M EE     P+   +   + ACA L A+   + +H Y  RN Q ++ + +   +IDMYAKCG I  AR +FD M  KN +TW++++  YG HG+G +A+ IF  M   G   + +T + +LYACSHSG+I++G+  F+ M   F V    +HY C+VDLLGRAGRL+ AL L+E M +E    +W A L  CRIHG ++L E A   + EL   + G Y LLSN+YA AGRWKDV ++R LM  + +KK PG +W+E    T  F  GD+ HP +++IY +L    ++++  GYVP+T F LHDVD+E K  +L+ HSEKLA+A+G++ T  G  IRITKNLRVCGDCHT   ++S +    II+RD +RFHHF+ G+CSC  YW
Sbjct:  250 QLHCFAVTSEMIQNMFVGNCLVDMYAKCGMMDEANTVFSNMSVKDVVSWNAMVAGYSQIGRFEDAVRLFEKMQEEKIKMDVVTWSAAISGYAQRGLGYEALGVCRQMLSSGIKPNEVTLISVLSGCASVGALMHGKEIHCYAIKYPIDLRKNGHGDENMVINQLIDMYAKCKKVDTARAMFDSLSPKERDVVTWTVMIGGYSQHGDANKALELLSEMFEEDCQTRPNAFTISCALVACASLAALRIGKQIHAYALRNQQNAVPLFVSNCLIDMYAKCGSISDARLVFDNMMAKNEVTWTSLMTGYGMHGYGEEALGIFDEMRRIGFKLDGVTLLVVLYACSHSGMIDQGMEYFNRMKTVFGVSPGPEHYACLVDLLGRAGRLNAALRLIEEMPMEPPPVVWVAFLSCCRIHGKVELGEYAAEKITELASNHDGSYTLLSNLYANAGRWKDVTRIRSLMRHKGVKKRPGCSWVEGIKGTTTFFVGDKTHPHAKEIYQVLLDHMQRIKDIGYVPETGFALHDVDDEEKDDLLFEHSEKLALAYGILTTPQGAAIRITKNLRVCGDCHTAFTYMSRIIDHDIILRDSSRFHHFKNGSCSCKGYW 850          

HSP 2 Score: 170.629 bits (431), Expect = 2.580e-41
Identity = 114/433 (26.33%), Postives = 204/433 (47.11%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVT--WSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEE-GVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAY---------------------------------GY--HGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADI-----------KHYTCMVDLLGRAGRLDEALELVESMA-VEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPEN 1152
            +H +++  G+L  L + + L+  Y     L  A +L       D+    W+ ++  + + G        F  +     TPD YT PFV + C ++  ++ G   H + L  G +++ F   ALV MY++C+ + DAR++FD+M   D+V+W  +I ++A+      +L +F  M  E G  PD + +V ++  CA LG     + LH +   ++   ++ +G  ++DMYAKCG +D A  +F  M VK+V++W+AM+A Y                                 GY   G G +A+ +   ML+SG+ PN +T +S+L  C+  G +  G  I       +A++  I                ++D+  +  ++D A  + +S++  E+D   W  ++G    HG+   A KA+  L E+  E+
Sbjct:   47 IHQKLLSFGIL-TLNLTSHLISTYISVGCLSHAVSLLRRFPPSDAGVYHWNSLIRSYGDNGCANKCLYLFGLMHSLSWTPDNYTFPFVFKACGEISSVRCGESAHALSLVTGFISNVFVGNALVAMYSRCRSLSDARKVFDEMSVWDVVSWNSIIESYAKLGKPKVALEMFSRMTNEFGCRPDNITLVNVLPPCASLGTHSLGKQLHCFAVTSEMIQNMFVGNCLVDMYAKCGMMDEANTVFSNMSVKDVVSWNAMVAGYSQIGRFEDAVRLFEKMQEEKIKMDVVTWSAAISGYAQRGLGYEALGVCRQMLSSGIKPNEVTLISVLSGCASVGALMHGKEIHC-----YAIKYPIDLRKNGHGDENMVINQLIDMYAKCKKVDTARAMFDSLSPKERDVVTWTVMIGGYSQHGD---ANKALELLSEMFEED 470          

BLAST of ppa025850m vs. ExPASy Swiss-Prot
Match: PP251_ARATH (Putative pentatricopeptide repeat-containing protein At3g23330 OS=Arabidopsis thaliana GN=PCMP-H32 PE=3 SV=1)

HSP 1 Score: 464.922 bits (1195), Expect = 6.617e-130
Identity = 213/518 (41.12%), Postives = 335/518 (64.67%), Query Frame = 1

Query:  109 LFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +F  M  +D V+++ ++ G+   G + +     RE+  + + PD +TL  V+ +  +  D+  G+ +H  V++ G+ +D +  ++LVDMYAK   ++D+ ++F ++  RD ++W  ++  + +    +E+L LF  M+   V P  VA  +++ ACA L  +H  + LH Y+ R  F  ++ + +A++DMY+KCG I +AR+IFDRM V + ++W+A+I  +  HGHG +A+++F  M   GV PN + FV++L ACSH GL++E    F+ M   + +  +++HY  + DLLGRAG+L+EA   +  M VE    +W  LL +C +H N++LAEK    +  +  EN G YVL+ N+YA  GRWK++AK+R  M ++ L+K P  +WIE+ NKT+ F +GDR HP  +KI   L+++ +++E  GYV DT+ VLHDVDEE K  +L+ HSE+LA+AFG+I T  GT IR+TKN+R+C DCH   KF+S +T+R IIVRD +RFHHF  G CSCGDYW
Sbjct:  198 VFEVMPRKDVVSYNTIIAGYAQSGMYEDALRMVREMGTTDLKPDSFTLSSVLPIFSEYVDVIKGKEIHGYVIRKGIDSDVYIGSSLVDMYAKSARIEDSERVFSRLYCRDGISWNSLVAGYVQNGRYNEALRLFRQMVTAKVKPGAVAFSSVIPACAHLATLHLGKQLHGYVLRGGFGSNIFIASALVDMYSKCGNIKAARKIFDRMNVLDEVSWTAIIMGHALHGHGHEAVSLFEEMKRQGVKPNQVAFVAVLTACSHVGLVDEAWGYFNSMTKVYGLNQELEHYAAVADLLGRAGKLEEAYNFISKMCVEPTGSVWSTLLSSCSVHKNLELAEKVAEKIFTVDSENMGAYVLMCNMYASNGRWKEMAKLRLRMRKKGLRKKPACSWIEMKNKTHGFVSGDRSHPSMDKINEFLKAVMEQMEKEGYVADTSGVLHDVDEEHKRELLFGHSERLAVAFGIINTEPGTTIRVTKNIRICTDCHVAIKFISKITEREIIVRDNSRFHHFNRGNCSCGDYW 715          

HSP 2 Score: 149.443 bits (376), Expect = 6.154e-35
Identity = 94/402 (23.38%), Postives = 195/402 (48.51%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKC-----KV-------------------------------VDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGN 1098
            ++HAQ +    L + T A+ ++ +Y   ++L +A  LF  +     + W  ++  F +   F+   A+F E+  SG  PD    P V++ C  M DL+ G  VH  +++ G+  D +   AL++MYAK      K+                               +D  R++F+ M  +D+V++  +I  +A+    +++L +   M    + PD   + +++   ++   + + + +H Y+ R     DV +G++++DMYAK   I+ +  +F R+  ++ I+W++++A Y  +G   +A+ +F  M+ + V P  + F S++ AC+H   +  G ++   +       ++I   + +VD+  + G +  A ++ + M V  DE  W A++    +HG+
Sbjct:   26 QLHAQFIRTQSLSH-TSASIVISIYTNLKLLHEALLLFKTLKSPPVLAWKSVIRCFTDQSLFSKALASFVEMRASGRCPDHNVFPSVLKSCTMMMDLRFGESVHGFIVRLGMDCDLYTGNALMNMYAKLLGMGSKISVGNVFDEMPQRTSNSGDEDVKAETCIMPFGIDSVRRVFEVMPRKDVVSYNTIIAGYAQSGMYEDALRMVREMGTTDLKPDSFTLSSVLPIFSEYVDVIKGKEIHGYVIRKGIDSDVYIGSSLVDMYAKSARIEDSERVFSRLYCRDGISWNSLVAGYVQNGRYNEALRLFRQMVTAKVKPGAVAFSSVIPACAHLATLHLGKQLHGYVL-RGGFGSNIFIASALVDMYSKCGNIKAARKIFDRMNV-LDEVSWTAIIMGHALHGH 424          

HSP 3 Score: 131.339 bits (329), Expect = 1.734e-29
Identity = 75/264 (28.41%), Postives = 140/264 (53.03%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILG--TAMIDMYAKCGCIDSAREIFDRMQVKNV-ITWSAMIAAYGYH 783
            E+H  V+  G+  ++ + + L+ MYA+   + D+  +F  +  RD ++W+ +V G+V  G +      FR+++ + V P       VI  C  +  L +G+ +H  VL+ G  ++ F  +ALVDMY+KC  +  AR++FD+M   D V+WT +I   A   +  E++ LF+ M  +GV P++VA V ++ AC+ +G +  A   ++      + L+  L    A+ D+  + G ++ A     +M V+     WS ++++   H
Sbjct:  263 EIHGYVIRKGIDSDVYIGSSLVDMYAKSARIEDSERVFSRLYCRDGISWNSLVAGYVQNGRYNEALRLFRQMVTAKVKPGAVAFSSVIPACAHLATLHLGKQLHGYVLRGGFGSNIFIASALVDMYSKCGNIKAARKIFDRMNVLDEVSWTAIIMGHALHGHGHEAVSLFEEMKRQGVKPNQVAFVAVLTACSHVGLVDEA-WGYFNSMTKVYGLNQELEHYAAVADLLGRAGKLEEAYNFISKMCVEPTGSVWSTLLSSCSVH 525          

BLAST of ppa025850m vs. ExPASy Swiss-Prot
Match: PPR21_ARATH (Pentatricopeptide repeat-containing protein At1g08070 OS=Arabidopsis thaliana GN=PCMP-H12 PE=2 SV=1)

HSP 1 Score: 463.381 bits (1191), Expect = 1.925e-129
Identity = 224/536 (41.79%), Postives = 342/536 (63.81%), Query Frame = 1

Query:   61 LLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVI--LGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            L+  YA R  + +A  LF  +  +D V+W+ M+ G+   G++      F++++++ V PD  T+  V+  C     +++GR VH  +  HG  ++     AL+D+Y+KC  ++ A  LF+++  +D+++W  +IG +       E+L+LF  M+  G  P+ V M++I+ ACA LGA+   R +H YI +    +     L T++IDMYAKCG I++A ++F+ +  K++ +W+AMI  +  HG    +  +F  M   G+ P+ ITFV LL ACSHSG+++ G  IF  M  ++ +   ++HY CM+DLLG +G   EA E++  M +E D  +W +LL AC++HGN++L E    +L++++PEN G YVLLSNIYA AGRW +VAK R L++ + +KKVPG + IE+D+  ++F  GD+ HP++ +IY MLE +   LE AG+VPDT+ VL +++EE K   L  HSEKLAIAFGLI+T  GT + I KNLRVC +CH  TK +S + +R II RD  RFHHFR+G CSC DYW
Sbjct:  206 LIKGYASRGYIENAQKLFDEIPVKDVVSWNAMISGYAETGNYKEALELFKDMMKTNVRPDESTMVTVVSACAQSGSIELGRQVHLWIDDHGFGSNLKIVNALIDLYSKCGELETACGLFERLPYKDVISWNTLIGGYTHMNLYKEALLLFQEMLRSGETPNDVTMLSILPACAHLGAIDIGRWIHVYIDKRLKGVTNASSLRTSLIDMYAKCGDIEAAHQVFNSILHKSLSSWNAMIFGFAMHGRADASFDLFSRMRKIGIQPDDITFVGLLSACSHSGMLDLGRHIFRTMTQDYKMTPKLEHYGCMIDLLGHSGLFKEAEEMINMMEMEPDGVIWCSLLKACKMHGNVELGESFAENLIKIEPENPGSYVLLSNIYASAGRWNEVAKTRALLNDKGMKKVPGCSSIEIDSVVHEFIIGDKFHPRNREIYGMLEEMEVLLEKAGFVPDTSEVLQEMEEEWKEGALRHHSEKLAIAFGLISTKPGTKLTIVKNLRVCRNCHEATKLISKIYKREIIARDRTRFHHFRDGVCSCNDYW 741          

HSP 2 Score: 182.956 bits (463), Expect = 5.023e-45
Identity = 121/428 (28.27%), Postives = 214/428 (50.00%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLL-YMYAQRRVLGDAYAL--FGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVT-------------------------------WTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEF-AVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSL--LELQPENAGHYVLLS 1176
            +HAQ++  G+       +KL+ +        G  YA+  F  + E + + W+ M  G     D  +    +  +I  G+ P+ YT PFV++ C   K  K G+ +H  VLK G   D +   +L+ MY +   ++DA ++FDK  +RD+V+                               W  MI  +AE  N  E+L LF  M++  V PD+  MVT+V+ACA+ G++   R +H +I  + F  ++ +  A+ID+Y+KCG +++A  +F+R+  K+VI+W+ +I  Y +    ++A+ +F  ML SG +PN +T +S+L AC+H G I+ G  I   +      V       T ++D+  + G ++ A ++  S+ + K    W A++    +HG  D +    + +  + +QP++     LLS
Sbjct:   52 IHAQMIKIGLHNTNYALSKLIEFCILSPHFEGLPYAISVFKTIQEPNLLIWNTMFRGHALSSDPVSALKLYVCMISLGLLPNSYTFPFVLKSCAKSKAFKEGQQIHGHVLKLGCDLDLYVHTSLISMYVQNGRLEDAHKVFDKSPHRDVVSYTALIKGYASRGYIENAQKLFDEIPVKDVVSWNAMISGYAETGNYKEALELFKDMMKTNVRPDESTMVTVVSACAQSGSIELGRQVHLWIDDHGFGSNLKIVNALIDLYSKCGELETACGLFERLPYKDVISWNTLIGGYTHMNLYKEALLLFQEMLRSGETPNDVTMLSILPACAHLGAIDIGRWIHVYIDKRLKGVTNASSLRTSLIDMYAKCGDIEAAHQVFNSI-LHKSLSSWNAMIFGFAMHGRADASFDLFSRMRKIGIQPDDITFVGLLS 478          

HSP 3 Score: 124.02 bits (310), Expect = 2.769e-27
Identity = 71/267 (26.59%), Postives = 141/267 (52.81%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKH--GLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRN-QFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVK-NVITWSAMIAAYGYHGH 789
            +VH  +  +G   NL + N L+ +Y++   L  A  LF  +  +D ++W+ ++GG+ ++  +      F+E++RSG TP+  T+  ++  C  +  + +GR +H  + K   G+   +    +L+DMYAKC  ++ A Q+F+ + ++ L +W  MI  FA    AD S  LF  M + G+ PD +  V +++AC+  G +   R +   + ++ + +  +     MID+    G    A E+ + M+++ + + W +++ A   HG+
Sbjct:  287 QVHLWIDDHGFGSNLKIVNALIDLYSKCGELETACGLFERLPYKDVISWNTLIGGYTHMNLYKEALLLFQEMLRSGETPNDVTMLSILPACAHLGAIDIGRWIHVYIDKRLKGVTNASSLRTSLIDMYAKCGDIEAAHQVFNSILHKSLSSWNAMIFGFAMHGRADASFDLFSRMRKIGIQPDDITFVGLLSACSHSGMLDLGRHIFRTMTQDYKMTPKLEHYGCMIDLLGHSGLFKEAEEMINMMEMEPDGVIWCSLLKACKMHGN 553          

HSP 4 Score: 116.316 bits (290), Expect = 5.773e-25
Identity = 78/306 (25.49%), Postives = 150/306 (49.02%), Query Frame = 1

Query:  262 IRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVD---MYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDM-------------------------------YAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLG 1077
            + +  + K L+  R++H  ++K GL   N+A + L++   +    + +  A  +F  +Q  +L+ W  M    A   +   +L L+  MI  G++P+      ++ +CAK  A    + +H ++ +    LD+ + T++I M                               YA  G I++A+++FD + VK+V++W+AMI+ Y   G+ ++A+ +F  M+ + V P+  T V+++ AC+ SG IE G ++  L  D+    +++K    ++DL  + G L+ A  L E +   KD   W  L+G
Sbjct:   37 LSLLHNCKTLQSLRIIHAQMIKIGLHNTNYALSKLIEFCILSPHFEGLPYAISVFKTIQEPNLLIWNTMFRGHALSSDPVSALKLYVCMISLGLLPNSYTFPFVLKSCAKSKAFKEGQQIHGHVLKLGCDLDLYVHTSLISMYVQNGRLEDAHKVFDKSPHRDVVSYTALIKGYASRGYIENAQKLFDEIPVKDVVSWNAMISGYAETGNYKEALELFKDMMKTNVRPDESTMVTVVSACAQSGSIELGRQV-HLWIDDHGFGSNLKIVNALIDLYSKCGELETACGLFERLPY-KDVISWNTLIG 340          

BLAST of ppa025850m vs. ExPASy Swiss-Prot
Match: PP320_ARATH (Pentatricopeptide repeat-containing protein At4g18750, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H45 PE=2 SV=1)

HSP 1 Score: 459.144 bits (1180), Expect = 3.631e-128
Identity = 223/537 (41.53%), Postives = 341/537 (63.50%), Query Frame = 1

Query:   55 NKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEG-VVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            N LL MY++   L  A A+F  M +R  V+++ M+ G+   G        F E+   G++PD+YT+  V+  C   + L  G+ VH+ + ++ L  D F   AL+DMYAKC  + +A  +F +M+ +D+++W  +IG +++   A+E+L LF+ ++EE    PD+  +  ++ ACA L A  + R +H YI RN +  D  +  +++DMYAKCG +  A  +FD +  K++++W+ MIA YG HG G++AIA+F+ M  +G+  + I+FVSLLYACSHSGL++EG R F++M  E  +   ++HY C+VD+L R G L +A   +E+M +  D  +WGALL  CRIH ++ LAEK    + EL+PEN G+YVL++NIYA A +W+ V ++R  + QR L+K PG +WIE+  +   F  GD  +P++E I A L  +  ++   GY P T + L D +E  K   L  HSEKLA+A G+I++  G  IR+TKNLRVCGDCH   KF+S +T+R I++RD NRFH F++G CSC  +W
Sbjct:  335 NTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPACASLSAFDKGREIHGYIMRNGYFSDRHVANSLVDMYAKCGALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKIEPTVEHYACIVDMLARTGDLIKAYRFIENMPIPPDATIWGALLCGCRIHHDVKLAEKVAEKVFELEPENTGYYVLMANIYAEAEKWEQVKRLRKRIGQRGLRKNPGCSWIEIKGRVNIFVAGDSSNPETENIEAFLRKVRARMIEEGYSPLTKYALIDAEEMEKEEALCGHSEKLAMALGIISSGHGKIIRVTKNLRVCGDCHEMAKFMSKLTRREIVLRDSNRFHQFKDGHCSCRGFW 871          

HSP 2 Score: 199.519 bits (506), Expect = 5.186e-50
Identity = 121/437 (27.69%), Postives = 223/437 (51.03%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLML-NSGVSPNVITFVSLLYACSHSGLIEEG-------------------------------LRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESM---AVEKDERLWGALLGACRIHGNIDLAEKAVNSL---LELQPENAGHYVLLSNIYARAG 1197
            ++H  ++ +G  +  +V N L+  Y + + +  A  +F  M ERD ++W+ ++ G+V+ G    G + F +++ SG+  D+ T+  V   C D + + +GR VH I +K     ++  C  L+DMY+KC  +D A+ +F +M +R +V++T MI  +A    A E++ LF+ M EEG+ PD   +  ++N CA+   +   + +H +I  N    D+ +  A++DMYAKCG +  A  +F  M+VK++I+W+ +I  Y  + +  +A+++F+L+L     SP+  T   +L AC+     ++G                               L +  +++D+ A + D+  +T M+   G  G   EA+ L   M    +E DE  + +LL AC   G +D   +  N +    +++P    HY  + ++ AR G
Sbjct:  216 QLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPACASLSAFDKGREIHGYIMRNGYFSDRHVANSLVDMYAKCGALLLAHMLFDDIASK-DLVSWTVMIAGYGMHGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKIEP-TVEHYACIVDMLARTG 650          

HSP 3 Score: 172.94 bits (437), Expect = 5.198e-42
Identity = 106/371 (28.57%), Postives = 187/371 (50.40%), Query Frame = 1

Query:   25 NGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLE 1137
            NG + +  + +KL  MY     L +A  +F  +    ++ W++++      GDF+     F++++ SGV  D YT   V +    ++ +  G  +H  +LK G    N    +LV  Y K + VD AR++FD+M  RD+++W  +I  +     A++ L +F  M+  G+  D   +V++   CA    +   R +H    +  FS +      ++DMY+KCG +DSA+ +F  M  ++V+++++MIA Y   G   +A+ +F  M   G+SP+V T  ++L  C+   L++EG R+   +  E  +  DI     ++D+  + G + EA ELV S    KD   W  ++G    +   + A    N LLE
Sbjct:  123 NGFVIDSNLGSKLSLMYTNCGDLKEASRVFDEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVEMDSYTFSCVSKSFSSLRSVHGGEQLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWI-KENDLGFDIFVSNALMDMYAKCGSMQEA-ELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLE 491          

HSP 4 Score: 125.176 bits (313), Expect = 1.243e-27
Identity = 82/304 (26.97%), Postives = 150/304 (49.34%), Query Frame = 1

Query:  238 DIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPE 1149
            D  TL  V+++C D K LK G+ V + +  +G + D+   + L  MY  C  + +A ++FD+++    + W +++   A+  +   S+ LF  M+  GV  D      +  + + L ++H    LH +I ++ F     +G +++  Y K   +DSAR++FD M  ++VI+W+++I  Y  +G   K +++F  ML SG+  ++ T VS+   C+ S LI  G  + S+       R D +    ++D+  + G LD A  +   M+          + G  R      LA +AV    E++ E
Sbjct:   93 DPRTLCSVLQLCADSKSLKDGKEVDNFIRGNGFVIDSNLGSKLSLMYTNCGDLKEASRVFDEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVEMDSYTFSCVSKSFSSLRSVHGGEQLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFSRED-RFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAR----EGLAGEAVKLFEEMEEE 391          

BLAST of ppa025850m vs. ExPASy Swiss-Prot
Match: PP296_ARATH (Pentatricopeptide repeat-containing protein At3g63370 OS=Arabidopsis thaliana GN=PCMP-H83 PE=2 SV=2)

HSP 1 Score: 459.144 bits (1180), Expect = 3.631e-128
Identity = 230/555 (41.44%), Postives = 351/555 (63.24%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLEL-AGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            E+HA V+ +G   NL V N L+ MY++  +       F  M ++D ++W+ ++ G+            FR++ +  +  D   L  ++R    +K + + + +H  +L+ GLL D      LVD+Y KC+ +  A ++F+ ++ +D+V+WT MI + A   N  E++ LF  M+E G+  D VA++ I++A A L A+++ R +H Y+ R  F L+  +  A++DMYA CG + SA+ +FDR++ K ++ +++MI AYG HG G+ A+ +F  M +  VSP+ I+F++LLYACSH+GL++EG     +M  E+ +    +HY C+VD+LGRA  + EA E V+ M  E    +W ALL ACR H   ++ E A   LLEL+P+N G+ VL+SN++A  GRW DV K+R  M    ++K PG +WIE+D K ++F+  D+ HP+S++IY  L  + +KLE   GYV DT FVLH+VDE  K+ ML+ HSE++AIA+GL+ T D   +RITKNLRVC DCHTF K VS + +R I++RD NRFHHF  G CSCGD W
Sbjct:  407 ELHAYVIKHGWDSNLQVGNTLIDMYSKCNLTCYMGRAFLRMHDKDLISWTTVIAGYAQNDCHVEALELFRDVAKKRMEIDEMILGSILRASSVLKSMLIVKEIHCHILRKGLL-DTVIQNELVDVYGKCRNMGYATRVFESIKGKDVVSWTSMISSSALNGNESEAVELFRRMVETGLSADSVALLCILSAAASLSALNKGREIHCYLLRKGFCLEGSIAVAVVDMYACCGDLQSAKAVFDRIERKGLLQYTSMINAYGMHGCGKAAVELFDKMRHENVSPDHISFLALLYACSHAGLLDEGRGFLKIMEHEYELEPWPEHYVCLVDMLGRANCVVEAFEFVKMMKTEPTAEVWCALLAACRSHSEKEIGEIAAQRLLELEPKNPGNLVLVSNVFAEQGRWNDVEKVRAKMKASGMEKHPGCSWIEMDGKVHKFTARDKSHPESKEIYEKLSEVTRKLEREVGYVADTKFVLHNVDEGEKVQMLHGHSERIAIAYGLLRTPDRACLRITKNLRVCRDCHTFCKLVSKLFRRDIVMRDANRFHHFESGLCSCGDSW 960          

HSP 2 Score: 144.436 bits (363), Expect = 1.980e-33
Identity = 101/392 (25.77%), Postives = 186/392 (47.45%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGER-DSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADN-FACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACS-----------HSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLE 1137
            E+H+ +V  G      + N L+ MYA+   L  A  LF G  E+ D+V W+ ++  +   G        FRE+  +G  P+ YT+   +  C      K+G+ +H  VLK    +   + C AL+ MY +C  +  A ++  +M N D+VTW  +I  + +     E+L  F  MI  G   D+V+M +I+ A  +L  +     LH Y+ ++ +  ++ +G  +IDMY+KC         F RM  K++I+W+ +IA Y  +    +A+ +F  +    +  + +   S+L A S           H  ++ +GL + +++ +E            +VD+ G+   +  A  + ES+   KD   W +++ +  ++GN   A +    ++E
Sbjct:  203 ELHSLLVKLGYHSTGFIVNALVSMYAKNDDLSAARRLFDGFQEKGDAVLWNSILSSYSTSGKSLETLELFREMHMTGPAPNSYTIVSALTACDGFSYAKLGKEIHASVLKSSTHSSELYVCNALIAMYTRCGKMPQAERILRQMNNADVVTWNSLIKGYVQNLMYKEALEFFSDMIAAGHKSDEVSMTSIIAASGRLSNLLAGMELHAYVIKHGWDSNLQVGNTLIDMYSKCNLTCYMGRAFLRMHDKDLISWTTVIAGYAQNDCHVEALELFRDVAKKRMEIDEMILGSILRASSVLKSMLIVKEIHCHILRKGL-LDTVIQNE------------LVDVYGKCRNMGYATRVFESIK-GKDVVSWTSMISSSALNGNESEAVELFRRMVE 580          

HSP 3 Score: 133.265 bits (334), Expect = 4.564e-30
Identity = 83/305 (27.21%), Postives = 156/305 (51.15%), Query Frame = 1

Query:   49 VANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNR-DLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNAC-----AKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLM----WD 933
            +A KL++MY +   L DA  +F  M +R +  W+ M+G +V+ G+  +  A +  +   GV   + + P +++ C  ++D++ G  +H +++K G  +  F   ALV MYAK   +  AR+LFD  Q + D V W  ++ +++    + E+L LF  M   G  P+   +V+ + AC     AKLG    A +L      +  S ++ +  A+I MY +CG +  A  I  +M   +V+TW+++I  Y  +   ++A+  F  M+ +G   + ++  S++ A      +  G+ + + +    WD
Sbjct:  118 LAGKLVFMYGKCGSLDDAEKVFDEMPDRTAFAWNTMIGAYVSNGEPASALALYWNMRVEGVPLGLSSFPALLKACAKLRDIRSGSELHSLLVKLGYHSTGFIVNALVSMYAKNDDLSAARRLFDGFQEKGDAVLWNSILSSYSTSGKSLETLELFREMHMTGPAPNSYTIVSALTACDGFSYAKLGKEIHASVLK----SSTHSSELYVCNALIAMYTRCGKMPQAERILRQMNNADVVTWNSLIKGYVQNLMYKEALEFFSDMIAAGHKSDEVSMTSIIAASGRLSNLLAGMELHAYVIKHGWD 418          

HSP 4 Score: 114.39 bits (285), Expect = 2.194e-24
Identity = 75/292 (25.68%), Postives = 136/292 (46.58%), Query Frame = 1

Query:  205 FRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLAD-NFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVK-NVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALL 1074
            F+ L  S     +    +V+ +C   + +  GR +H  + K     + +F    LV MY KC  +DDA ++FD+M +R    W  MIGA+        +L L+  M  EGV     +   ++ ACAKL  +     LH  + +  +     +  A++ MYAK   + +AR +FD  Q K + + W++++++Y   G   + + +F  M  +G +PN  T VS L AC      + G  I + +       +++     ++ +  R G++ +A  ++  M    D   W +L+
Sbjct:   68 FQRLDVSENNSPVEAFAYVLELCGKRRAVSQGRQLHSRIFKTFPSFELDFLAGKLVFMYGKCGSLDDAEKVFDEMPDRTAFAWNTMIGAYVSNGEPASALALYWNMRVEGVPLGLSSFPALLKACAKLRDIRSGSELHSLLVKLGYHSTGFIVNALVSMYAKNDDLSAARRLFDGFQEKGDAVLWNSILSSYSTSGKSLETLELFREMHMTGPAPNSYTIVSALTACDGFSYAKLGKEIHASVLKSSTHSSELYVCNALIAMYTRCGKMPQAERILRQMN-NADVVTWNSLI 358          

BLAST of ppa025850m vs. TAIR10
Match: AT4G33990.1 (| Symbols: EMB2758 | Tetratricopeptide repeat (TPR)-like superfamily protein | chr4:16290141-16292612 REVERSE LENGTH=823)

HSP 1 Score: 481.871 bits (1239), Expect = 4.716e-136
Identity = 236/555 (42.52%), Postives = 362/555 (65.23%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACA-ALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEG-VVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +H+  + +G+   L V+NKL+ +YA+   L D   +F  M  RD ++W+ ++  +          + F+E+  S + PD  TL  +  +   + D++  R V    L+ G   ++     A+V MYAK  +VD AR +F+ + N D+++W  +I  +A+   A E++ +++ M EEG +  ++   V+++ AC++ GA+ +   LH  + +N   LDV + T++ DMY KCG ++ A  +F ++   N + W+ +IA +G+HGHG KA+ +F  ML+ GV P+ ITFV+LL ACSHSGL++EG   F +M  ++ +   +KHY CMVD+ GRAG+L+ AL+ ++SM+++ D  +WGALL ACR+HGN+DL + A   L E++PE+ G++VLLSN+YA AG+W+ V ++R +   + L+K PG + +EVDNK   F TG++ HP  E++Y  L +L  KL++ GYVPD  FVL DV+++ K  +L SHSE+LAIAF LIAT   T IRI KNLRVCGDCH+ TKF+S +T+R IIVRD NRFHHF+ G CSCGDYW
Sbjct:  269 IHSYSIKHGLESELFVSNKLIDLYAEFGRLRDCQKVFDRMYVRDLISWNSIIKAYELNEQPLRAISLFQEMRLSRIQPDCLTLISLASILSQLGDIRACRSVQGFTLRKGWFLEDITIGNAVVVMYAKLGLVDSARAVFNWLPNTDVISWNTIISGYAQNGFASEAIEMYNIMEEEGEIAANQGTWVSVLPACSQAGALRQGMKLHGRLLKNGLYLDVFVVTSLADMYGKCGRLEDALSLFYQIPRVNSVPWNTLIACHGFHGHGEKAVMLFKEMLDEGVKPDHITFVTLLSACSHSGLVDEGQWCFEMMQTDYGITPSLKHYGCMVDMYGRAGQLETALKFIKSMSLQPDASIWGALLSACRVHGNVDLGKIASEHLFEVEPEHVGYHVLLSNMYASAGKWEGVDEIRSIAHGKGLRKTPGWSSMEVDNKVEVFYTGNQTHPMYEEMYRELTALQAKLKMIGYVPDHRFVLQDVEDDEKEHILMSHSERLAIAFALIATPAKTTIRIFKNLRVCGDCHSVTKFISKITEREIIVRDSNRFHHFKNGVCSCGDYW 823          

HSP 2 Score: 172.17 bits (435), Expect = 7.993e-43
Identity = 112/410 (27.32%), Postives = 209/410 (50.98%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRE-LIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPE---NAGHYVLLSNIYARAGRWKDVAKM 1221
            +HA++VV+  +QN+ ++ KL+ +Y     +  A   F  +  RD   W++M+ G+   G+ +     F   ++ SG+TPD  T P V++ CR + D   G  +H + LK G + D +  A+L+ +Y++ K V +AR LFD+M  RD+ +W  MI  + +  NA E+L L + +       D V +V++++AC + G  +R   +H Y  ++    ++ +   +ID+YA+ G +   +++FDRM V+++I+W+++I AY  +    +AI++F  M  S + P+ +T +SL    S  G I     +      +     DI     +V +  + G +D A   V +     D   W  ++     +G    A +  N ++E + E   N G +V +    ++AG  +   K+
Sbjct:   73 LHARLVVSKQIQNVCISAKLVNLYCYLGNVALARHTFDHIQNRDVYAWNLMISGYGRAGNSSEVIRCFSLFMLSSGLTPDYRTFPSVLKACRTVID---GNKIHCLALKFGFMWDVYVAASLIHLYSRYKAVGNARILFDEMPVRDMGSWNAMISGYCQSGNAKEALTLSNGLR----AMDSVTVVSLLSACTEAGDFNRGVTIHSYSIKHGLESELFVSNKLIDLYAEFGRLRDCQKVFDRMYVRDLISWNSIIKAYELNEQPLRAISLFQEMRLSRIQPDCLTLISLASILSQLGDIRACRSVQGFTLRKGWFLEDITIGNAVVVMYAKLGLVDSA-RAVFNWLPNTDVISWNTIISGYAQNGFASEAIEMYN-IMEEEGEIAANQGTWVSVLPACSQAGALRQGMKL 473          

HSP 3 Score: 69.707 bits (169), Expect = 5.587e-12
Identity = 55/198 (27.78%), Postives = 92/198 (46.46%), Query Frame = 1

Query:  559 TIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHL-MLNSGVSPNVITFVSLLYACSHSGLIEEGLRI------FSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSL 1131
            T+   C  L +   A+ LH  +  ++   +V +   ++++Y   G +  AR  FD +Q ++V  W+ MI+ YG  G+  + I  F L ML+SG++P+  TF S+L AC     + +G +I      F  MWD +   +       ++ L  R   +  A  L + M V +D   W A++      GN   A    N L
Sbjct:   59 TLFRYCTNLQS---AKCLHARLVVSKQIQNVCISAKLVNLYCYLGNVALARHTFDHIQNRDVYAWNLMISGYGRAGNSSEVIRCFSLFMLSSGLTPDYRTFPSVLKACR---TVIDGNKIHCLALKFGFMWDVYVAAS-------LIHLYSRYKAVGNARILFDEMPV-RDMGSWNAMISGYCQSGNAKEALTLSNGL 242          

BLAST of ppa025850m vs. TAIR10
Match: AT1G11290.1 (| Symbols: CRR22 | Pentatricopeptide repeat (PPR) superfamily protein | chr1:3791454-3793883 REVERSE LENGTH=809)

HSP 1 Score: 477.248 bits (1227), Expect = 1.162e-134
Identity = 238/554 (42.96%), Postives = 348/554 (62.82%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            E+H   + +G    + ++  L+ MYA+   L  A  LF GM ER+ V+W+ M+  +V   +       F++++  GV P   ++   +  C D+ DL+ GR +H + ++ GL  +     +L+ MY KCK VD A  +F K+Q+R LV+W  MI  FA+     ++L  F  M    V PD    V+++ A A+L   H A+ +H  + R+    +V + TA++DMYAKCG I  AR IFD M  ++V TW+AMI  YG HG G+ A+ +F  M    + PN +TF+S++ ACSHSGL+E GL+ F +M + +++   + HY  MVDLLGRAGRL+EA + +  M V+    ++GA+LGAC+IH N++ AEKA   L EL P++ G++VLL+NIY  A  W+ V ++R  M ++ L+K PG + +E+ N+ + F +G   HP S+KIYA LE L   ++ AGYVPDTN VL  V+ +VK  +L +HSEKLAI+FGL+ T  GT I + KNLRVC DCH  TK++S VT R I+VRD+ RFHHF+ G CSCGDYW
Sbjct:  257 EIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEVDTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITAIAELSITHHAKWIHGVVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMSERHVTTWNAMIDGYGTHGFGKAALELFEEMQKGTIKPNGVTFLSVISACSHSGLVEAGLKCFYMMKENYSIELSMDHYGAMVDLLGRAGRLNEAWDFIMQMPVKPAVNVYGAMLGACQIHKNVNFAEKAAERLFELNPDDGGYHVLLANIYRAASMWEKVGQVRVSMLRQGLRKTPGCSMVEIKNEVHSFFSGSTAHPDSKKIYAFLEKLICHIKEAGYVPDTNLVL-GVENDVKEQLLSTHSEKLAISFGLLNTTAGTTIHVRKNLRVCADCHNATKYISLVTGREIVVRDMQRFHHFKNGACSCGDYW 809          

HSP 2 Score: 191.815 bits (486), Expect = 9.749e-49
Identity = 97/303 (32.01%), Postives = 175/303 (57.76%), Query Frame = 1

Query:   16 VVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSL 924
            V  NG+ Q      KL+ ++ +   + +A  +F  +  + +V +  M+ GF  V D       F  +    V P +Y   ++++VC D  +L++G+ +H +++K G   D FA   L +MYAKC+ V++AR++FD+M  RDLV+W  ++  +++   A  +L +   M EE + P  + +V+++ A + L  +   + +H Y  R+ F   V + TA++DMYAKCG +++AR++FD M  +NV++W++MI AY  + + ++A+ IF  ML+ GV P  ++ +  L+AC+  G +E G  I  L
Sbjct:   60 VFKNGLYQEHFFQTKLVSLFCRYGSVDEAARVFEPIDSKLNVLYHTMLKGFAKVSDLDKALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVGKEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKPTDVSVMGALHACADLGDLERGRFIHKL 362          

HSP 3 Score: 179.874 bits (455), Expect = 3.834e-45
Identity = 103/365 (28.22%), Postives = 186/365 (50.96%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            E+H  +V +G   +L     L  MYA+ R + +A  +F  M ERD V+W+ +V G+   G         + +    + P   T+  V+     ++ + +G+ +H   ++ G  +      ALVDMYAKC  ++ ARQLFD M  R++V+W  MI A+ +  N  E++++F  M++EGV P  V+++  ++ACA LG + R R +H          +V +  ++I MY KC  +D+A  +F ++Q + +++W+AMI  +  +G    A+  F  M +  V P+  T+VS++ A +   +      I  ++     +  ++   T +VD+  + G +  A  L+  M  E+    W A++     HG
Sbjct:  156 EIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKPTDVSVMGALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEVDTAASMFGKLQSRTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITAIAELSITHHAKWIHGVVM-RSCLDKNVFVTTALVDMYAKCGAIMIA-RLIFDMMSERHVTTWNAMIDGYGTHG 518          

HSP 4 Score: 125.176 bits (313), Expect = 1.121e-28
Identity = 78/293 (26.62%), Postives = 153/293 (52.22%), Query Frame = 1

Query:  220 RSGVTPDIYTLP--FVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRAD----IKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGA 1080
            R+ +  ++Y  P   ++  C  +K+L   R +  +V K+GL  ++F    LV ++ +   VD+A ++F+ + ++  V +  M+  FA+  + D++L  F  M  + V P       ++  C     +   + +H  + ++ FSLD+   T + +MYAKC  ++ AR++FDRM  +++++W+ ++A Y  +G  R A+ +   M    + P+ IT VS+L A S   LI  G  I       +A+R+     +   T +VD+  + G L+ A +L + M +E++   W +++ A
Sbjct:   28 RNYIPANVYEHPAALLLERCSSLKEL---RQILPLVFKNGLYQEHFFQTKLVSLFCRYGSVDEAARVFEPIDSKLNVLYHTMLKGFAKVSDLDKALQFFVRMRYDDVEPVVYNFTYLLKVCGDEAELRVGKEIHGLLVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMALEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHG-----YAMRSGFDSLVNISTALVDMYAKCGSLETARQLFDGM-LERNVVSWNSMIDA 311          

BLAST of ppa025850m vs. TAIR10
Match: AT3G46790.1 (| Symbols: CRR2 | Tetratricopeptide repeat (TPR)-like superfamily protein | chr3:17231975-17233948 REVERSE LENGTH=657)

HSP 1 Score: 474.167 bits (1219), Expect = 9.834e-134
Identity = 235/559 (42.04%), Postives = 353/559 (63.15%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCR----DMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEE--GVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            VH  ++ NG  Q+  +A KL+ MY+    +  A  +F    +R    W+ +       G        + ++ R GV  D +T  +V++ C      +  L  G+ +H  + + G  +  +    LVDMYA+   VD A  +F  M  R++V+W+ MI  +A+   A E+L  F  M+ E     P+ V MV+++ ACA L A+ + +L+H YI R      + + +A++ MY +CG ++  + +FDRM  ++V++W+++I++YG HG+G+KAI IF  ML +G SP  +TFVS+L ACSH GL+EEG R+F  MW +  ++  I+HY CMVDLLGRA RLDEA ++V+ M  E   ++WG+LLG+CRIHGN++LAE+A   L  L+P+NAG+YVLL++IYA A  W +V +++ L+  R L+K+PG  W+EV  K Y F + D  +P  E+I+A L  L + ++  GY+P T  VL++++ E K  ++  HSEKLA+AFGLI T+ G PIRITKNLR+C DCH FTKF+S   ++ I+VRDVNRFH F+ G CSCGDYW
Sbjct:   99 VHRHILDNGSDQDPFLATKLIGMYSDLGSVDYARKVFDKTRKRTIYVWNALFRALTLAGHGEEVLGLYWKMNRIGVESDRFTYTYVLKACVASECTVNHLMKGKEIHAHLTRRGYSSHVYIMTTLVDMYARFGCVDYASYVFGGMPVRNVVSWSAMIACYAKNGKAFEALRTFREMMRETKDSSPNSVTMVSVLQACASLAALEQGKLIHGYILRRGLDSILPVISALVTMYGRCGKLEVGQRVFDRMHDRDVVSWNSLISSYGVHGYGKKAIQIFEEMLANGASPTPVTFVSVLGACSHEGLVEEGKRLFETMWRDHGIKPQIEHYACMVDLLGRANRLDEAAKMVQDMRTEPGPKVWGSLLGSCRIHGNVELAERASRRLFALEPKNAGNYVLLADIYAEAQMWDEVKRVKKLLEHRGLQKLPGRCWMEVRRKMYSFVSVDEFNPLMEQIHAFLVKLAEDMKEKGYIPQTKGVLYELETEEKERIVLGHSEKLALAFGLINTSKGEPIRITKNLRLCEDCHLFTKFISKFMEKEILVRDVNRFHRFKNGVCSCGDYW 657          

HSP 2 Score: 139.428 bits (350), Expect = 5.742e-33
Identity = 89/314 (28.34%), Postives = 152/314 (48.41%), Query Frame = 1

Query:  232 TPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNAC----AKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLML--NSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENA 1155
            +P   T   +I  C     L     VH  +L +G   D F    L+ MY+    VD AR++FDK + R +  W  +  A     + +E L L+  M   GV  D+     ++ AC      +  + + + +H ++ R  +S  V + T ++DMYA+ GC+D A  +F  M V+NV++WSAMIA Y  +G   +A+  F  M+      SPN +T VS+L AC+    +E+G  I   +     + + +   + +V + GR G+L+    + + M  ++D   W +L+ +  +HG     +KA+    E+    A
Sbjct:   74 SPSQQTYELLILCCGHRSSLSDALRVHRHILDNGSDQDPFLATKLIGMYSDLGSVDYARKVFDKTRKRTIYVWNALFRALTLAGHGEEVLGLYWKMNRIGVESDRFTYTYVLKACVASECTVNHLMKGKEIHAHLTRRGYSSHVYIMTTLVDMYARFGCVDYASYVFGGMPVRNVVSWSAMIACYAKNGKAFEALRTFREMMRETKDSSPNSVTMVSVLQACASLAALEQGKLIHGYILRR-GLDSILPVISALVTMYGRCGKLEVGQRVFDRMH-DRDVVSWNSLISSYGVHG---YGKKAIQIFEEMLANGA 382          

HSP 3 Score: 122.094 bits (305), Expect = 9.486e-28
Identity = 68/267 (25.47%), Postives = 137/267 (51.31%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIR--SGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQ-FSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVK-NVITWSAMIAAYGYHGH 789
            E+HA +   G   ++ +   L+ MYA+   +  A  +FGGM  R+ V+WS M+  +   G       TFRE++R     +P+  T+  V++ C  +  L+ G+L+H  +L+ GL +     +ALV MY +C  ++  +++FD+M +RD+V+W  +I ++       +++ +F+ M+  G  P  V  V+++ AC+  G +   + L   + R+      +     M+D+  +   +D A ++   M+ +     W +++ +   HG+
Sbjct:  203 EIHAHLTRRGYSSHVYIMTTLVDMYARFGCVDYASYVFGGMPVRNVVSWSAMIACYAKNGKAFEALRTFREMMRETKDSSPNSVTMVSVLQACASLAALEQGKLIHGYILRRGLDSILPVISALVTMYGRCGKLEVGQRVFDRMHDRDVVSWNSLISSYGVHGYGKKAIQIFEEMLANGASPTPVTFVSVLGACSHEGLVEEGKRLFETMWRDHGIKPQIEHYACMVDLLGRANRLDEAAKMVQDMRTEPGPKVWGSLLGSCRIHGN 469          

BLAST of ppa025850m vs. TAIR10
Match: AT3G57430.1 (| Symbols: OTP84 | Tetratricopeptide repeat (TPR)-like superfamily protein | chr3:21255731-21258403 REVERSE LENGTH=890)

HSP 1 Score: 472.626 bits (1215), Expect = 2.861e-133
Identity = 236/568 (41.55%), Postives = 357/568 (62.85%), Query Frame = 1

Query:    1 EVHAQVVVNGML-QNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRS-GVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWM------IEEG-----VVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDER-LWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            E+HA  + NG L +N  V + L+ MY   + +     +F GM +R    W+ M+ G+            F  +  S G+  +  T+  V+  C           +H  V+K GL  D F    L+DMY++   +D A ++F KM++RDLVTW  MI  +    + +++L+L   M      + +G     + P+ + ++TI+ +CA L A+ + + +H Y  +N  + DV +G+A++DMYAKCGC+  +R++FD++  KNVITW+ +I AYG HG+G++AI +  +M+  GV PN +TF+S+  ACSHSG+++EGLRIF +M  ++ V     HY C+VDLLGRAGR+ EA +L+  M  + ++   W +LLGA RIH N+++ E A  +L++L+P  A HYVLL+NIY+ AG W    ++R  M ++ ++K PG +WIE  ++ ++F  GD  HPQSEK+   LE+L +++   GYVPDT+ VLH+V+E+ K  +L  HSEKLAIAFG++ T+ GT IR+ KNLRVC DCH  TKF+S +  R II+RDV RFH F+ GTCSCGDYW
Sbjct:  323 ELHAYALKNGSLDENSFVGSALVDMYCNCKQVLSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTMAGVVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMEDRDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRVSLKPNSITLMTILPSCAALSALAKGKEIHAYAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVFDQIPQKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHSGMVDEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEAYQLMNMMPRDFNKAGAWSSLLGASRIHNNLEIGEIAAQNLIQLEPNVASHYVLLANIYSSAGLWDKATEVRRNMKEQGVRKEPGCSWIEHGDEVHKFVAGDSSHPQSEKLSGYLETLWERMRKEGYVPDTSCVLHNVEEDEKEILLCGHSEKLAIAFGILNTSPGTIIRVAKNLRVCNDCHLATKFISKIVDREIILRDVRRFHRFKNGTCSCGDYW 890          

HSP 2 Score: 182.956 bits (463), Expect = 4.528e-46
Identity = 122/465 (26.24%), Postives = 221/465 (47.53%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADN-FACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEE-GVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLN-----------SGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVR----ADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGN----IDL----------------------------AEKAVNSLLELQPE-----NAGHYVLLSNIYARAGRWKDVAKMRDLM 1233
            +VHA  +  G L N  + N L+ MY +   L  +  L G  G RD VTW+ ++                RE++  GV PD +T+  V+  C  ++ L+ G+ +H   LK+G L +N F  +ALVDMY  CK V   R++FD M +R +  W  MI  +++  +  E+L+LF  M E  G++ +   M  +V AC + GA  R   +H ++ +     D  +   ++DMY++ G ID A  IF +M+ ++++TW+ MI  Y +  H   A+ + H M N             + PN IT +++L +C+    + +G  I +     +A++     D+   + +VD+  + G L  + ++ + +  +K+   W  ++ A  +HGN    IDL                             ++ +     ++P+     ++ HY  + ++  RAGR K+  ++ ++M
Sbjct:  223 QVHAYGLRKGEL-NSFIINTLVAMYGKLGKLASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLEALEYLREMVLEGVEPDEFTISSVLPACSHLEMLRTGKELHAYALKNGSLDENSFVGSALVDMYCNCKQVLSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTMAGVVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTLMDMYSRLGKIDIAMRIFGKMEDRDLVTWNTMITGYVFSEHHEDALLLLHKMQNLERKVSKGASRVSLKPNSITLMTILPSCAALSALAKGKEIHA-----YAIKNNLATDVAVGSALVDMYAKCGCLQMSRKVFDQIP-QKNVITWNVIIMAYGMHGNGQEAIDLLRMMMVQGVKPNEVTFISVFAACSHSGMVDEGLRIFYVMKPDYGVEPSSDHYACVVDLLGRAGRIKEAYQLMNMM 680          

HSP 3 Score: 153.68 bits (387), Expect = 2.942e-37
Identity = 105/365 (28.77%), Postives = 178/365 (48.77%), Query Frame = 1

Query:    1 EVHAQVVVNGM-LQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDM---KDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLD--VILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNS-GVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALL 1074
            ++HA V   G  + ++TVAN L+ +Y +    G  Y +F  + ER+ V+W+ ++    +   +      FR ++   V P  +TL  V+  C ++   + L MG+ VH   L+ G L ++F    LV MY K   +  ++ L      RDLVTW  ++ +  +     E+L     M+ EGV PD+  + +++ AC+ L  +   + LH Y  +N  SLD    +G+A++DMY  C  + S R +FD M  + +  W+AMIA Y  + H ++A+ +F  M  S G+  N  T   ++ AC  SG       I   +      R      T M D+  R G++D A+ +   M  ++D   W  ++
Sbjct:  118 QIHAHVYKFGYGVDSVTVANTLVNLYRKCGDFGAVYKVFDRISERNQVSWNSLISSLCSFEKWEMALEAFRCMLDENVEPSSFTLVSVVTACSNLPMPEGLMMGKQVHAYGLRKGEL-NSFIINTLVAMYGKLGKLASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLEALEYLREMVLEGVEPDEFTISSVLPACSHLEMLRTGKELHAYALKNG-SLDENSFVGSALVDMYCNCKQVLSGRRVFDGMFDRKIGLWNAMIAGYSQNEHDKEALLLFIGMEESAGLLANSTTMAGVVPACVRSGAFSRKEAIHGFVVKRGLDRDRFVQNTLM-DMYSRLGKIDIAMRIFGKME-DRDLVTWNTMI 478          

HSP 4 Score: 111.694 bits (278), Expect = 1.282e-24
Identity = 69/295 (23.39%), Postives = 145/295 (49.15%), Query Frame = 1

Query:  202 TFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACA-ALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKL---GAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALL 1074
            T+ ++I  G+ PD Y  P +++   D++D+++G+ +H  V K G   D+   A  LV++Y KC       ++FD++  R+ V+W  +I +       + +L  F  M++E V P    +V++V AC+ L     +   + +H Y  R       I+ T ++ MY K G + S++ +      ++++TW+ ++++   +    +A+     M+  GV P+  T  S+L ACSH  ++  G  + +      ++  +    + +VD+     ++     + + M  ++   LW A++
Sbjct:   84 TYVDMIVLGIKPDNYAFPALLKAVADLQDMELGKQIHAHVYKFGYGVDSVTVANTLVNLYRKCGDFGAVYKVFDRISERNQVSWNSLISSLCSFEKWEMALEAFRCMLDENVEPSSFTLVSVVTACSNLPMPEGLMMGKQVHAYGLRKGELNSFIINT-LVAMYGKLGKLASSKVLLGSFGGRDLVTWNTVLSSLCQNEQLLEALEYLREMVLEGVEPDEFTISSVLPACSHLEMLRTGKELHAYALKNGSLDENSFVGSALVDMYCNCKQVLSGRRVFDGM-FDRKIGLWNAMI 376          

HSP 5 Score: 77.0258 bits (188), Expect = 3.500e-14
Identity = 51/221 (23.08%), Postives = 108/221 (48.87%), Query Frame = 1

Query:  427 QNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLD-VILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHY--TCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGA 1080
            Q+R    W  ++ +        E+++ +  MI  G+ PD  A   ++ A A L  M   + +H ++ +  + +D V +   ++++Y KCG   +  ++FDR+  +N ++W+++I++         A+  F  ML+  V P+  T VS++ ACS+  +  EGL +   +      + ++  +    +V + G+ G+L  +  L+ S    +D   W  +L +
Sbjct:   58 QSRSPEWWIDLLRSKVRSNLLREAVLTYVDMIVLGIKPDNYAFPALLKAVADLQDMELGKQIHAHVYKFGYGVDSVTVANTLVNLYRKCGDFGAVYKVFDRISERNQVSWNSLISSLCSFEKWEMALEAFRCMLDENVEPSSFTLVSVVTACSNLPM-PEGLMMGKQVHAYGLRKGELNSFIINTLVAMYGKLGKLASSKVLLGSFG-GRDLVTWNTVLSS 276          

BLAST of ppa025850m vs. TAIR10
Match: AT3G12770.1 (| Symbols: MEF22 | mitochondrial editing factor 22 | chr3:4057027-4059193 REVERSE LENGTH=694)

HSP 1 Score: 468.774 bits (1205), Expect = 4.132e-132
Identity = 233/555 (41.98%), Postives = 349/555 (62.88%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMG--ERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            VHAQV   G   ++ V N L+ +YA+ R LG A  +F G+   ER  V+W+ +V  +   G+       F ++ +  V PD   L  V+     ++DLK GR +H  V+K GL  +     +L  MYAKC  V  A+ LFDKM++ +L+ W  MI  +A+   A E++ +F  MI + V PD +++ + ++ACA++G++ +AR ++ Y+ R+ +  DV + +A+IDM+AKCG ++ AR +FDR   ++V+ WSAMI  YG HG  R+AI+++  M   GV PN +TF+ LL AC+HSG++ EG   F+ M D   +    +HY C++DLLGRAG LD+A E+++ M V+    +WGALL AC+ H +++L E A   L  + P N GHYV LSN+YA A  W  VA++R  M ++ L K  G +W+EV  +   F  GD+ HP+ E+I   +E +  +L+  G+V + +  LHD+++E     L SHSE++AIA+GLI+T  GTP+RITKNLR C +CH  TK +S +  R I+VRD NRFHHF++G CSCGDYW
Sbjct:  141 VHAQVFRLGFDADVFVQNGLIALYAKCRRLGSARTVFEGLPLPERTIVSWTAIVSAYAQNGEPMEALEIFSQMRKMDVKPDWVALVSVLNAFTCLQDLKQGRSIHASVVKMGLEIEPDLLISLNTMYAKCGQVATAKILFDKMKSPNLILWNAMISGYAKNGYAREAIDMFHEMINKDVRPDTISITSAISACAQVGSLEQARSMYEYVGRSDYRDDVFISSALIDMFAKCGSVEGARLVFDRTLDRDVVVWSAMIVGYGLHGRAREAISLYRAMERGGVHPNDVTFLGLLMACNHSGMVREGWWFFNRMADH-KINPQQQHYACVIDLLGRAGHLDQAYEVIKCMPVQPGVTVWGALLSACKKHRHVELGEYAAQQLFSIDPSNTGHYVQLSNLYAAARLWDRVAEVRVRMKEKGLNKDVGCSWVEVRGRLEAFRVGDKSHPRYEEIERQVEWIESRLKEGGFVANKDASLHDLNDEEAEETLCSHSERIAIAYGLISTPQGTPLRITKNLRACVNCHAATKLISKLVDREIVVRDTNRFHHFKDGVCSCGDYW 694          

HSP 2 Score: 188.348 bits (477), Expect = 1.078e-47
Identity = 103/367 (28.07%), Postives = 199/367 (54.22%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFD--KMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            ++HA+++V G+  +  +  KL++  +    +  A  +F  +       W+ ++ G+     F +    +  +  + V+PD +T P +++ C  +  L+MGR VH  V + G  AD F    L+ +YAKC+ +  AR +F+   +  R +V+WT ++ A+A+     E+L +F  M +  V PD VA+V+++NA   L  + + R +H  + +    ++  L  ++  MYAKCG + +A+ +FD+M+  N+I W+AMI+ Y  +G+ R+AI +FH M+N  V P+ I+  S + AC+  G +E+   ++  +      R D+   + ++D+  + G + E   LV    +++D  +W A++    +HG
Sbjct:   39 QIHARLLVLGLQFSGFLITKLIHASSSFGDITFARQVFDDLPRPQIFPWNAIIRGYSRNNHFQDALLMYSNMQLARVSPDSFTFPHLLKACSGLSHLQMGRFVHAQVFRLGFDADVFVQNGLIALYAKCRRLGSARTVFEGLPLPERTIVSWTAIVSAYAQNGEPMEALEIFSQMRKMDVKPDWVALVSVLNAFTCLQDLKQGRSIHASVVKMGLEIEPDLLISLNTMYAKCGQVATAKILFDKMKSPNLILWNAMISGYAKNGYAREAIDMFHEMINKDVRPDTISITSAISACAQVGSLEQARSMYEYV-GRSDYRDDVFISSALIDMFAKCGSV-EGARLVFDRTLDRDVVVWSAMIVGYGLHG 403          

BLAST of ppa025850m vs. TAIR10
Match: AT5G16860.1 (| Symbols: | Tetratricopeptide repeat (TPR)-like superfamily protein | chr5:5543834-5546386 FORWARD LENGTH=850)

HSP 1 Score: 465.307 bits (1196), Expect = 4.568e-131
Identity = 241/601 (40.10%), Postives = 352/601 (58.57%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATF-----------------------------------RELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKH-------GLLADNFACAALVDMYAKCKVVDDARQLFDKM--QNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEG--VVPDKVAMVTIVNACAKLGAMHRARLLHYYICRN-QFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            ++H   V + M+QN+ V N L+ MYA+  ++ +A  +F  M  +D V+W+ MV G+  +G F +    F                                   R+++ SG+ P+  TL  V+  C  +  L  G+ +H   +K+       G   +N     L+DMYAKCK VD AR +FD +  + RD+VTWTVMIG +++  +A+++L L   M EE     P+   +   + ACA L A+   + +H Y  RN Q ++ + +   +IDMYAKCG I  AR +FD M  KN +TW++++  YG HG+G +A+ IF  M   G   + +T + +LYACSHSG+I++G+  F+ M   F V    +HY C+VDLLGRAGRL+ AL L+E M +E    +W A L  CRIHG ++L E A   + EL   + G Y LLSN+YA AGRWKDV ++R LM  + +KK PG +W+E    T  F  GD+ HP +++IY +L    ++++  GYVP+T F LHDVD+E K  +L+ HSEKLA+A+G++ T  G  IRITKNLRVCGDCHT   ++S +    II+RD +RFHHF+ G+CSC  YW
Sbjct:  250 QLHCFAVTSEMIQNMFVGNCLVDMYAKCGMMDEANTVFSNMSVKDVVSWNAMVAGYSQIGRFEDAVRLFEKMQEEKIKMDVVTWSAAISGYAQRGLGYEALGVCRQMLSSGIKPNEVTLISVLSGCASVGALMHGKEIHCYAIKYPIDLRKNGHGDENMVINQLIDMYAKCKKVDTARAMFDSLSPKERDVVTWTVMIGGYSQHGDANKALELLSEMFEEDCQTRPNAFTISCALVACASLAALRIGKQIHAYALRNQQNAVPLFVSNCLIDMYAKCGSISDARLVFDNMMAKNEVTWTSLMTGYGMHGYGEEALGIFDEMRRIGFKLDGVTLLVVLYACSHSGMIDQGMEYFNRMKTVFGVSPGPEHYACLVDLLGRAGRLNAALRLIEEMPMEPPPVVWVAFLSCCRIHGKVELGEYAAEKITELASNHDGSYTLLSNLYANAGRWKDVTRIRSLMRHKGVKKRPGCSWVEGIKGTTTFFVGDKTHPHAKEIYQVLLDHMQRIKDIGYVPETGFALHDVDDEEKDDLLFEHSEKLALAYGILTTPQGAAIRITKNLRVCGDCHTAFTYMSRIIDHDIILRDSSRFHHFKNGSCSCKGYW 850          

HSP 2 Score: 170.629 bits (431), Expect = 2.326e-42
Identity = 114/433 (26.33%), Postives = 204/433 (47.11%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVT--WSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEE-GVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAY---------------------------------GY--HGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADI-----------KHYTCMVDLLGRAGRLDEALELVESMA-VEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPEN 1152
            +H +++  G+L  L + + L+  Y     L  A +L       D+    W+ ++  + + G        F  +     TPD YT PFV + C ++  ++ G   H + L  G +++ F   ALV MY++C+ + DAR++FD+M   D+V+W  +I ++A+      +L +F  M  E G  PD + +V ++  CA LG     + LH +   ++   ++ +G  ++DMYAKCG +D A  +F  M VK+V++W+AM+A Y                                 GY   G G +A+ +   ML+SG+ PN +T +S+L  C+  G +  G  I       +A++  I                ++D+  +  ++D A  + +S++  E+D   W  ++G    HG+   A KA+  L E+  E+
Sbjct:   47 IHQKLLSFGIL-TLNLTSHLISTYISVGCLSHAVSLLRRFPPSDAGVYHWNSLIRSYGDNGCANKCLYLFGLMHSLSWTPDNYTFPFVFKACGEISSVRCGESAHALSLVTGFISNVFVGNALVAMYSRCRSLSDARKVFDEMSVWDVVSWNSIIESYAKLGKPKVALEMFSRMTNEFGCRPDNITLVNVLPPCASLGTHSLGKQLHCFAVTSEMIQNMFVGNCLVDMYAKCGMMDEANTVFSNMSVKDVVSWNAMVAGYSQIGRFEDAVRLFEKMQEEKIKMDVVTWSAAISGYAQRGLGYEALGVCRQMLSSGIKPNEVTLISVLSGCASVGALMHGKEIHC-----YAIKYPIDLRKNGHGDENMVINQLIDMYAKCKKVDTARAMFDSLSPKERDVVTWTVMIGGYSQHGD---ANKALELLSEMFEED 470          

BLAST of ppa025850m vs. TAIR10
Match: AT3G23330.1 (| Symbols: | Tetratricopeptide repeat (TPR)-like superfamily protein | chr3:8347200-8349347 FORWARD LENGTH=715)

HSP 1 Score: 464.922 bits (1195), Expect = 5.966e-131
Identity = 213/518 (41.12%), Postives = 335/518 (64.67%), Query Frame = 1

Query:  109 LFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +F  M  +D V+++ ++ G+   G + +     RE+  + + PD +TL  V+ +  +  D+  G+ +H  V++ G+ +D +  ++LVDMYAK   ++D+ ++F ++  RD ++W  ++  + +    +E+L LF  M+   V P  VA  +++ ACA L  +H  + LH Y+ R  F  ++ + +A++DMY+KCG I +AR+IFDRM V + ++W+A+I  +  HGHG +A+++F  M   GV PN + FV++L ACSH GL++E    F+ M   + +  +++HY  + DLLGRAG+L+EA   +  M VE    +W  LL +C +H N++LAEK    +  +  EN G YVL+ N+YA  GRWK++AK+R  M ++ L+K P  +WIE+ NKT+ F +GDR HP  +KI   L+++ +++E  GYV DT+ VLHDVDEE K  +L+ HSE+LA+AFG+I T  GT IR+TKN+R+C DCH   KF+S +T+R IIVRD +RFHHF  G CSCGDYW
Sbjct:  198 VFEVMPRKDVVSYNTIIAGYAQSGMYEDALRMVREMGTTDLKPDSFTLSSVLPIFSEYVDVIKGKEIHGYVIRKGIDSDVYIGSSLVDMYAKSARIEDSERVFSRLYCRDGISWNSLVAGYVQNGRYNEALRLFRQMVTAKVKPGAVAFSSVIPACAHLATLHLGKQLHGYVLRGGFGSNIFIASALVDMYSKCGNIKAARKIFDRMNVLDEVSWTAIIMGHALHGHGHEAVSLFEEMKRQGVKPNQVAFVAVLTACSHVGLVDEAWGYFNSMTKVYGLNQELEHYAAVADLLGRAGKLEEAYNFISKMCVEPTGSVWSTLLSSCSVHKNLELAEKVAEKIFTVDSENMGAYVLMCNMYASNGRWKEMAKLRLRMRKKGLRKKPACSWIEMKNKTHGFVSGDRSHPSMDKINEFLKAVMEQMEKEGYVADTSGVLHDVDEEHKRELLFGHSERLAVAFGIINTEPGTTIRVTKNIRICTDCHVAIKFISKITEREIIVRDNSRFHHFNRGNCSCGDYW 715          

HSP 2 Score: 149.443 bits (376), Expect = 5.548e-36
Identity = 94/402 (23.38%), Postives = 195/402 (48.51%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKC-----KV-------------------------------VDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGN 1098
            ++HAQ +    L + T A+ ++ +Y   ++L +A  LF  +     + W  ++  F +   F+   A+F E+  SG  PD    P V++ C  M DL+ G  VH  +++ G+  D +   AL++MYAK      K+                               +D  R++F+ M  +D+V++  +I  +A+    +++L +   M    + PD   + +++   ++   + + + +H Y+ R     DV +G++++DMYAK   I+ +  +F R+  ++ I+W++++A Y  +G   +A+ +F  M+ + V P  + F S++ AC+H   +  G ++   +       ++I   + +VD+  + G +  A ++ + M V  DE  W A++    +HG+
Sbjct:   26 QLHAQFIRTQSLSH-TSASIVISIYTNLKLLHEALLLFKTLKSPPVLAWKSVIRCFTDQSLFSKALASFVEMRASGRCPDHNVFPSVLKSCTMMMDLRFGESVHGFIVRLGMDCDLYTGNALMNMYAKLLGMGSKISVGNVFDEMPQRTSNSGDEDVKAETCIMPFGIDSVRRVFEVMPRKDVVSYNTIIAGYAQSGMYEDALRMVREMGTTDLKPDSFTLSSVLPIFSEYVDVIKGKEIHGYVIRKGIDSDVYIGSSLVDMYAKSARIEDSERVFSRLYCRDGISWNSLVAGYVQNGRYNEALRLFRQMVTAKVKPGAVAFSSVIPACAHLATLHLGKQLHGYVL-RGGFGSNIFIASALVDMYSKCGNIKAARKIFDRMNV-LDEVSWTAIIMGHALHGH 424          

HSP 3 Score: 131.339 bits (329), Expect = 1.564e-30
Identity = 75/264 (28.41%), Postives = 140/264 (53.03%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILG--TAMIDMYAKCGCIDSAREIFDRMQVKNV-ITWSAMIAAYGYH 783
            E+H  V+  G+  ++ + + L+ MYA+   + D+  +F  +  RD ++W+ +V G+V  G +      FR+++ + V P       VI  C  +  L +G+ +H  VL+ G  ++ F  +ALVDMY+KC  +  AR++FD+M   D V+WT +I   A   +  E++ LF+ M  +GV P++VA V ++ AC+ +G +  A   ++      + L+  L    A+ D+  + G ++ A     +M V+     WS ++++   H
Sbjct:  263 EIHGYVIRKGIDSDVYIGSSLVDMYAKSARIEDSERVFSRLYCRDGISWNSLVAGYVQNGRYNEALRLFRQMVTAKVKPGAVAFSSVIPACAHLATLHLGKQLHGYVLRGGFGSNIFIASALVDMYSKCGNIKAARKIFDRMNVLDEVSWTAIIMGHALHGHGHEAVSLFEEMKRQGVKPNQVAFVAVLTACSHVGLVDEA-WGYFNSMTKVYGLNQELEHYAAVADLLGRAGKLEEAYNFISKMCVEPTGSVWSTLLSSCSVH 525          

BLAST of ppa025850m vs. TAIR10
Match: AT1G08070.1 (| Symbols: OTP82 | Tetratricopeptide repeat (TPR)-like superfamily protein | chr1:2514374-2516599 REVERSE LENGTH=741)

HSP 1 Score: 463.381 bits (1191), Expect = 1.736e-130
Identity = 224/536 (41.79%), Postives = 342/536 (63.81%), Query Frame = 1

Query:   61 LLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVI--LGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            L+  YA R  + +A  LF  +  +D V+W+ M+ G+   G++      F++++++ V PD  T+  V+  C     +++GR VH  +  HG  ++     AL+D+Y+KC  ++ A  LF+++  +D+++W  +IG +       E+L+LF  M+  G  P+ V M++I+ ACA LGA+   R +H YI +    +     L T++IDMYAKCG I++A ++F+ +  K++ +W+AMI  +  HG    +  +F  M   G+ P+ ITFV LL ACSHSG+++ G  IF  M  ++ +   ++HY CM+DLLG +G   EA E++  M +E D  +W +LL AC++HGN++L E    +L++++PEN G YVLLSNIYA AGRW +VAK R L++ + +KKVPG + IE+D+  ++F  GD+ HP++ +IY MLE +   LE AG+VPDT+ VL +++EE K   L  HSEKLAIAFGLI+T  GT + I KNLRVC +CH  TK +S + +R II RD  RFHHFR+G CSC DYW
Sbjct:  206 LIKGYASRGYIENAQKLFDEIPVKDVVSWNAMISGYAETGNYKEALELFKDMMKTNVRPDESTMVTVVSACAQSGSIELGRQVHLWIDDHGFGSNLKIVNALIDLYSKCGELETACGLFERLPYKDVISWNTLIGGYTHMNLYKEALLLFQEMLRSGETPNDVTMLSILPACAHLGAIDIGRWIHVYIDKRLKGVTNASSLRTSLIDMYAKCGDIEAAHQVFNSILHKSLSSWNAMIFGFAMHGRADASFDLFSRMRKIGIQPDDITFVGLLSACSHSGMLDLGRHIFRTMTQDYKMTPKLEHYGCMIDLLGHSGLFKEAEEMINMMEMEPDGVIWCSLLKACKMHGNVELGESFAENLIKIEPENPGSYVLLSNIYASAGRWNEVAKTRALLNDKGMKKVPGCSSIEIDSVVHEFIIGDKFHPRNREIYGMLEEMEVLLEKAGFVPDTSEVLQEMEEEWKEGALRHHSEKLAIAFGLISTKPGTKLTIVKNLRVCRNCHEATKLISKIYKREIIARDRTRFHHFRDGVCSCNDYW 741          

HSP 2 Score: 182.956 bits (463), Expect = 4.528e-46
Identity = 121/428 (28.27%), Postives = 214/428 (50.00%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLL-YMYAQRRVLGDAYAL--FGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVT-------------------------------WTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEF-AVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSL--LELQPENAGHYVLLS 1176
            +HAQ++  G+       +KL+ +        G  YA+  F  + E + + W+ M  G     D  +    +  +I  G+ P+ YT PFV++ C   K  K G+ +H  VLK G   D +   +L+ MY +   ++DA ++FDK  +RD+V+                               W  MI  +AE  N  E+L LF  M++  V PD+  MVT+V+ACA+ G++   R +H +I  + F  ++ +  A+ID+Y+KCG +++A  +F+R+  K+VI+W+ +I  Y +    ++A+ +F  ML SG +PN +T +S+L AC+H G I+ G  I   +      V       T ++D+  + G ++ A ++  S+ + K    W A++    +HG  D +    + +  + +QP++     LLS
Sbjct:   52 IHAQMIKIGLHNTNYALSKLIEFCILSPHFEGLPYAISVFKTIQEPNLLIWNTMFRGHALSSDPVSALKLYVCMISLGLLPNSYTFPFVLKSCAKSKAFKEGQQIHGHVLKLGCDLDLYVHTSLISMYVQNGRLEDAHKVFDKSPHRDVVSYTALIKGYASRGYIENAQKLFDEIPVKDVVSWNAMISGYAETGNYKEALELFKDMMKTNVRPDESTMVTVVSACAQSGSIELGRQVHLWIDDHGFGSNLKIVNALIDLYSKCGELETACGLFERLPYKDVISWNTLIGGYTHMNLYKEALLLFQEMLRSGETPNDVTMLSILPACAHLGAIDIGRWIHVYIDKRLKGVTNASSLRTSLIDMYAKCGDIEAAHQVFNSI-LHKSLSSWNAMIFGFAMHGRADASFDLFSRMRKIGIQPDDITFVGLLS 478          

HSP 3 Score: 124.02 bits (310), Expect = 2.496e-28
Identity = 71/267 (26.59%), Postives = 141/267 (52.81%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKH--GLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRN-QFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVK-NVITWSAMIAAYGYHGH 789
            +VH  +  +G   NL + N L+ +Y++   L  A  LF  +  +D ++W+ ++GG+ ++  +      F+E++RSG TP+  T+  ++  C  +  + +GR +H  + K   G+   +    +L+DMYAKC  ++ A Q+F+ + ++ L +W  MI  FA    AD S  LF  M + G+ PD +  V +++AC+  G +   R +   + ++ + +  +     MID+    G    A E+ + M+++ + + W +++ A   HG+
Sbjct:  287 QVHLWIDDHGFGSNLKIVNALIDLYSKCGELETACGLFERLPYKDVISWNTLIGGYTHMNLYKEALLLFQEMLRSGETPNDVTMLSILPACAHLGAIDIGRWIHVYIDKRLKGVTNASSLRTSLIDMYAKCGDIEAAHQVFNSILHKSLSSWNAMIFGFAMHGRADASFDLFSRMRKIGIQPDDITFVGLLSACSHSGMLDLGRHIFRTMTQDYKMTPKLEHYGCMIDLLGHSGLFKEAEEMINMMEMEPDGVIWCSLLKACKMHGN 553          

HSP 4 Score: 116.316 bits (290), Expect = 5.205e-26
Identity = 78/306 (25.49%), Postives = 150/306 (49.02%), Query Frame = 1

Query:  262 IRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVD---MYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDM-------------------------------YAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLG 1077
            + +  + K L+  R++H  ++K GL   N+A + L++   +    + +  A  +F  +Q  +L+ W  M    A   +   +L L+  MI  G++P+      ++ +CAK  A    + +H ++ +    LD+ + T++I M                               YA  G I++A+++FD + VK+V++W+AMI+ Y   G+ ++A+ +F  M+ + V P+  T V+++ AC+ SG IE G ++  L  D+    +++K    ++DL  + G L+ A  L E +   KD   W  L+G
Sbjct:   37 LSLLHNCKTLQSLRIIHAQMIKIGLHNTNYALSKLIEFCILSPHFEGLPYAISVFKTIQEPNLLIWNTMFRGHALSSDPVSALKLYVCMISLGLLPNSYTFPFVLKSCAKSKAFKEGQQIHGHVLKLGCDLDLYVHTSLISMYVQNGRLEDAHKVFDKSPHRDVVSYTALIKGYASRGYIENAQKLFDEIPVKDVVSWNAMISGYAETGNYKEALELFKDMMKTNVRPDESTMVTVVSACAQSGSIELGRQV-HLWIDDHGFGSNLKIVNALIDLYSKCGELETACGLFERLPY-KDVISWNTLIG 340          

BLAST of ppa025850m vs. TAIR10
Match: AT4G18750.1 (| Symbols: DOT4 | Pentatricopeptide repeat (PPR) superfamily protein | chr4:10304850-10307465 FORWARD LENGTH=871)

HSP 1 Score: 459.144 bits (1180), Expect = 3.274e-129
Identity = 223/537 (41.53%), Postives = 341/537 (63.50%), Query Frame = 1

Query:   55 NKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEG-VVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            N LL MY++   L  A A+F  M +R  V+++ M+ G+   G        F E+   G++PD+YT+  V+  C   + L  G+ VH+ + ++ L  D F   AL+DMYAKC  + +A  +F +M+ +D+++W  +IG +++   A+E+L LF+ ++EE    PD+  +  ++ ACA L A  + R +H YI RN +  D  +  +++DMYAKCG +  A  +FD +  K++++W+ MIA YG HG G++AIA+F+ M  +G+  + I+FVSLLYACSHSGL++EG R F++M  E  +   ++HY C+VD+L R G L +A   +E+M +  D  +WGALL  CRIH ++ LAEK    + EL+PEN G+YVL++NIYA A +W+ V ++R  + QR L+K PG +WIE+  +   F  GD  +P++E I A L  +  ++   GY P T + L D +E  K   L  HSEKLA+A G+I++  G  IR+TKNLRVCGDCH   KF+S +T+R I++RD NRFH F++G CSC  +W
Sbjct:  335 NTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPACASLSAFDKGREIHGYIMRNGYFSDRHVANSLVDMYAKCGALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKIEPTVEHYACIVDMLARTGDLIKAYRFIENMPIPPDATIWGALLCGCRIHHDVKLAEKVAEKVFELEPENTGYYVLMANIYAEAEKWEQVKRLRKRIGQRGLRKNPGCSWIEIKGRVNIFVAGDSSNPETENIEAFLRKVRARMIEEGYSPLTKYALIDAEEMEKEEALCGHSEKLAMALGIISSGHGKIIRVTKNLRVCGDCHEMAKFMSKLTRREIVLRDSNRFHQFKDGHCSCRGFW 871          

HSP 2 Score: 199.519 bits (506), Expect = 4.675e-51
Identity = 121/437 (27.69%), Postives = 223/437 (51.03%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLML-NSGVSPNVITFVSLLYACSHSGLIEEG-------------------------------LRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESM---AVEKDERLWGALLGACRIHGNIDLAEKAVNSL---LELQPENAGHYVLLSNIYARAG 1197
            ++H  ++ +G  +  +V N L+  Y + + +  A  +F  M ERD ++W+ ++ G+V+ G    G + F +++ SG+  D+ T+  V   C D + + +GR VH I +K     ++  C  L+DMY+KC  +D A+ +F +M +R +V++T MI  +A    A E++ LF+ M EEG+ PD   +  ++N CA+   +   + +H +I  N    D+ +  A++DMYAKCG +  A  +F  M+VK++I+W+ +I  Y  + +  +A+++F+L+L     SP+  T   +L AC+     ++G                               L +  +++D+ A + D+  +T M+   G  G   EA+ L   M    +E DE  + +LL AC   G +D   +  N +    +++P    HY  + ++ AR G
Sbjct:  216 QLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPACASLSAFDKGREIHGYIMRNGYFSDRHVANSLVDMYAKCGALLLAHMLFDDIASK-DLVSWTVMIAGYGMHGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKIEP-TVEHYACIVDMLARTG 650          

HSP 3 Score: 172.94 bits (437), Expect = 4.686e-43
Identity = 106/371 (28.57%), Postives = 187/371 (50.40%), Query Frame = 1

Query:   25 NGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLE 1137
            NG + +  + +KL  MY     L +A  +F  +    ++ W++++      GDF+     F++++ SGV  D YT   V +    ++ +  G  +H  +LK G    N    +LV  Y K + VD AR++FD+M  RD+++W  +I  +     A++ L +F  M+  G+  D   +V++   CA    +   R +H    +  FS +      ++DMY+KCG +DSA+ +F  M  ++V+++++MIA Y   G   +A+ +F  M   G+SP+V T  ++L  C+   L++EG R+   +  E  +  DI     ++D+  + G + EA ELV S    KD   W  ++G    +   + A    N LLE
Sbjct:  123 NGFVIDSNLGSKLSLMYTNCGDLKEASRVFDEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVEMDSYTFSCVSKSFSSLRSVHGGEQLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWI-KENDLGFDIFVSNALMDMYAKCGSMQEA-ELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLLLE 491          

HSP 4 Score: 125.176 bits (313), Expect = 1.121e-28
Identity = 82/304 (26.97%), Postives = 150/304 (49.34%), Query Frame = 1

Query:  238 DIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPE 1149
            D  TL  V+++C D K LK G+ V + +  +G + D+   + L  MY  C  + +A ++FD+++    + W +++   A+  +   S+ LF  M+  GV  D      +  + + L ++H    LH +I ++ F     +G +++  Y K   +DSAR++FD M  ++VI+W+++I  Y  +G   K +++F  ML SG+  ++ T VS+   C+ S LI  G  + S+       R D +    ++D+  + G LD A  +   M+          + G  R      LA +AV    E++ E
Sbjct:   93 DPRTLCSVLQLCADSKSLKDGKEVDNFIRGNGFVIDSNLGSKLSLMYTNCGDLKEASRVFDEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVEMDSYTFSCVSKSFSSLRSVHGGEQLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFSRED-RFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAR----EGLAGEAVKLFEEMEEE 391          

HSP 5 Score: 54.299 bits (129), Expect = 2.429e-7
Identity = 38/145 (26.21%), Postives = 70/145 (48.28%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIV---LKHGLLADNFACAALVDMYAKCKVVDDARQLFDKM 426
            E+H  ++ NG   +  VAN L+ MYA+   L  A+ LF  +  +D V+W+VM+ G+   G      A F ++ ++G+  D  +   ++  C     +  G    +I+    K     +++AC  +VDM A+   +  A +  + M
Sbjct:  520 EIHGYIMRNGYFSDRHVANSLVDMYAKCGALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKIEPTVEHYAC--IVDMLARTGDLIKAYRFIENM 662          

BLAST of ppa025850m vs. TAIR10
Match: AT4G30700.1 (| Symbols: | Pentatricopeptide repeat (PPR) superfamily protein | chr4:14962617-14964995 REVERSE LENGTH=792)

HSP 1 Score: 457.218 bits (1175), Expect = 1.244e-128
Identity = 229/554 (41.34%), Postives = 336/554 (60.65%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            ++H+     G   +  V    + +Y++   +    ALF    + D V ++ M+ G+ + G+     + F+EL+ SG      TL  ++ V      L +   +H   LK   L+      AL  +Y+K   ++ AR+LFD+   + L +W  MI  + +    ++++ LF  M +    P+ V +  I++ACA+LGA+   + +H  +    F   + + TA+I MYAKCG I  AR +FD M  KN +TW+ MI+ YG HG G++A+ IF+ MLNSG++P  +TF+ +LYACSH+GL++EG  IF+ M   +     +KHY CMVD+LGRAG L  AL+ +E+M++E    +W  LLGACRIH + +LA      L EL P+N G++VLLSNI++    +   A +R    +R+L K PG T IE+    + F++GD+ HPQ ++IY  LE L  K+  AGY P+T   LHDV+EE +  M+  HSE+LAIAFGLIAT  GT IRI KNLRVC DCHT TK +S +T+RVI+VRD NRFHHF++G CSCGDYW
Sbjct:  242 QIHSLATKTGCYSHDYVLTGFISLYSKCGKIKMGSALFREFRKPDIVAYNAMIHGYTSNGETELSLSLFKELMLSGARLRSSTLVSLVPVSGH---LMLIYAIHGYCLKSNFLSHASVSTALTTVYSKLNEIESARKLFDESPEKSLPSWNAMISGYTQNGLTEDAISLFREMQKSEFSPNPVTITCILSACAQLGALSLGKWVHDLVRSTDFESSIYVSTALIGMYAKCGSIAEARRLFDLMTKKNEVTWNTMISGYGLHGQGQEALNIFYEMLNSGITPTPVTFLCVLYACSHAGLVKEGDEIFNSMIHRYGFEPSVKHYACMVDILGRAGHLQRALQFIEAMSIEPGSSVWETLLGACRIHKDTNLARTVSEKLFELDPDNVGYHVLLSNIHSADRNYPQAATVRQTAKKRKLAKAPGYTLIEIGETPHVFTSGDQSHPQVKEIYEKLEKLEGKMREAGYQPETELALHDVEEEERELMVKVHSERLAIAFGLIATEPGTEIRIIKNLRVCLDCHTVTKLISKITERVIVVRDANRFHHFKDGVCSCGDYW 792          

HSP 2 Score: 113.235 bits (282), Expect = 4.406e-25
Identity = 92/384 (23.96%), Postives = 177/384 (46.09%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRS-GVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVP-DKVAMVTIVNACAKLGAMHRARLLHYYICRNQ-FSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQ 1143
            + HAQ++++G   ++++  KL    +    +  A  +F  +   D   ++V++ GF       +  + F  L +S  + P+  T  F I      +D + GR++H   +  G  ++    + +V MY K   V+DAR++FD+M  +D + W  MI  + +     ES+ +F  +I E     D   ++ I+ A A+L  +     +H    +   +S D +L T  I +Y+KCG I     +F   +  +++ ++AMI  Y  +G    ++++F  ++ SG      T VSL+    H  LI   +  + L    F   A +   T +  +  +   ++ A +L +  + EK    W A++     +G   L E A++   E+Q
Sbjct:   38 QTHAQIILHGFRNDISLLTKLTQRLSDLGAIYYARDIFLSVQRPDVFLFNVLMRGFSVNESPHSSLSVFAHLRKSTDLKPNSSTYAFAISAASGFRDDRAGRVIHGQAVVDGCDSELLLGSNIVKMYFKFWRVEDARKVFDRMPEKDTILWNTMISGYRKNEMYVESIQVFRDLINESCTRLDTTTLLDILPAVAELQELRLGMQIHSLATKTGCYSHDYVL-TGFISLYSKCGKIKMGSALFREFRKPDIVAYNAMIHGYTSNGETELSLSLFKELMLSGARLRSSTLVSLVPVSGHLMLI-YAIHGYCLK-SNFLSHASVS--TALTTVYSKLNEIESARKLFDE-SPEKSLPSWNAMISGYTQNG---LTEDAISLFREMQ 412          

BLAST of ppa025850m vs. Populus trichicarpa v2.0
Match: POPTR_0016s07840.1 ()

HSP 1 Score: 796.193 bits (2055), Expect = 0.000e+0
Identity = 383/554 (69.13%), Postives = 456/554 (82.31%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +VHAQV   G++ +L VANKLLYM A+ + L  A+ LF  M ERD V+WSVM+GGFV  GD+   F TFRELIR+G  PD ++LPFVI+ CRD   L MGRL+H  VLK+GL  DNF C+ LVDMYAKC ++D+A+QLFD+M  +DLVT TVMI  +AEC   +ES VLFD M  +G VPDKVAMVTIVNACAKLGAM++ARL+H Y+C  ++SLDV LGTAMIDMYAKCG IDS+REIFDRM+ KNVI+WSAMI AYGYHG GR+A+ +FH+MLNSG+ PN ITF+SLLYACSH+GL+++GL++FSLM   + VR D+KHYTCMVDLLGRAGRLD+AL L+E+M VEKDE +W A LGACRIH  +DLAEKA   LL LQ +N GHY+LLSNIYA AGRWKDVAK+R+LM++RRLKK+PG TWIEVDN  Y+F  GD  H +S++IY ML+SL +KLE AGYVPDTN VLHDVDEEVKL +L++HSEKLAIAFGLIAT DGTPIRITKNLRVCGDCH+F K VSA+TQR IIVRD NRFHHF+EG CSCGDYW
Sbjct:   21 QVHAQVTTTGIIHDLIVANKLLYMCAKHKDLVTAHLLFNKMEERDPVSWSVMIGGFVKNGDYERCFQTFRELIRAGSKPDNFSLPFVIKACRDTMGLIMGRLIHSTVLKNGLHLDNFVCSTLVDMYAKCGMIDNAKQLFDRMPKKDLVTRTVMIAGYAECGKPNESWVLFDQMRRDGFVPDKVAMVTIVNACAKLGAMNKARLVHDYVCARRYSLDVELGTAMIDMYAKCGSIDSSREIFDRMEQKNVISWSAMIGAYGYHGQGREALELFHMMLNSGIIPNRITFISLLYACSHAGLVDDGLQLFSLMSVSYGVRPDVKHYTCMVDLLGRAGRLDQALRLIENMEVEKDEGIWCAFLGACRIHRQVDLAEKAAKLLLSLQTQNPGHYILLSNIYANAGRWKDVAKIRNLMAKRRLKKIPGYTWIEVDNIIYRFGAGDNSHLRSKEIYEMLKSLSQKLESAGYVPDTNSVLHDVDEEVKLGILHAHSEKLAIAFGLIATPDGTPIRITKNLRVCGDCHSFCKLVSAITQRDIIVRDANRFHHFKEGICSCGDYW 574          

BLAST of ppa025850m vs. Populus trichicarpa v2.0
Match: POPTR_0018s00520.1 ()

HSP 1 Score: 494.967 bits (1273), Expect = 6.130e-140
Identity = 249/537 (46.37%), Postives = 351/537 (65.36%), Query Frame = 1

Query:   61 LLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDM-KDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLD--VILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            L+  YA +  L +A  LF  +  RD V+W+ M+ G+   G      A F E+ R+ VTP++ T+  V+  C      L++G  V   +   GL ++      L+DMY KC  +++A  LF+K+Q++++V+W VMIG +       E+L LF  M++  + P+ V  ++I+ ACA LGA+   + +H Y+ +N  S+   V L T++IDMYAKCG + +A+ IFD M  K++ TW+AMI+ +  HGH   A+ +F  M + G  P+ ITFV +L AC H+GL+  G R FS M  ++ V   + HY CM+DL GRAG  DEA  LV++M ++ D  +W +LLGACRIH  I+LAE     L EL+PEN   YVLLSNIYA AGRW+DVAK+R  ++  R+KKVPG + IEVD+  ++F  GD++HPQS +IY ML+ +  +LE AG+VPDT+ VL+D+DEE K  +L  HSEKLAIAFGLI+T  GT IRI KNLRVCG+CH+ TK +S +  R II RD NRFHHF++G+CSC DYW
Sbjct:  169 LITGYASKGFLDEARELFDEIPVRDVVSWNAMISGYAQSGRVEEAMAFFEEMRRAKVTPNVSTMLSVLSACAQSGSSLQLGNWVRSWIEDRGLGSNIRLVNGLIDMYVKCGDLEEASNLFEKIQDKNVVSWNVMIGGYTHMSCYKEALGLFRRMMQSNIDPNDVTFLSILPACANLGALDLGKWVHAYVDKNMKSMKNTVALWTSLIDMYAKCGDLAAAKRIFDCMNTKSLATWNAMISGFAMHGHTDTALGLFSRMTSEGFVPDDITFVGVLTACKHAGLLSLGRRYFSSMIQDYKVSPKLPHYGCMIDLFGRAGLFDEAETLVKNMEMKPDGAIWCSLLGACRIHRRIELAESVAKHLFELEPENPSAYVLLSNIYAGAGRWEDVAKIRTRLNDNRMKKVPGCSSIEVDSVVHEFLVGDKVHPQSNEIYKMLDEIDMRLEKAGFVPDTSEVLYDMDEEWKEGVLSHHSEKLAIAFGLISTKPGTTIRIMKNLRVCGNCHSATKLISKIFNREIIARDRNRFHHFKDGSCSCKDYW 705          

HSP 2 Score: 158.303 bits (399), Expect = 1.360e-38
Identity = 91/332 (27.41%), Postives = 172/332 (51.81%), Query Frame = 1

Query:  214 LIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAK------CKVV-------------------------DDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLG-AMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEF-AVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLA 1110
            +I SG  P+ YT P + + C  ++    G+ VH  VLK GL  + F   +L++MYA+       ++V                         D+AR+LFD++  RD+V+W  MI  +A+    +E++  F+ M    V P+   M+++++ACA+ G ++     +  +I       ++ L   +IDMY KCG ++ A  +F+++Q KNV++W+ MI  Y +    ++A+ +F  M+ S + PN +TF+S+L AC++ G ++ G  + + +     +++  +  +T ++D+  + G L  A  + + M   K    W A++    +HG+ D A
Sbjct:   88 MISSGTEPNEYTFPSIFKSCTKIRGAHEGKQVHAHVLKLGLEHNAFVHTSLINMYAQNGELVNARLVFDKSSMRDAVSFTALITGYASKGFLDEARELFDEIPVRDVVSWNAMISGYAQSGRVEEAMAFFEEMRRAKVTPNVSTMLSVLSACAQSGSSLQLGNWVRSWIEDRGLGSNIRLVNGLIDMYVKCGDLEEASNLFEKIQDKNVVSWNVMIGGYTHMSCYKEALGLFRRMMQSNIDPNDVTFLSILPACANLGALDLGKWVHAYVDKNMKSMKNTVALWTSLIDMYAKCGDLAAAKRIFDCMNT-KSLATWNAMISGFAMHGHTDTA 418          

HSP 3 Score: 129.798 bits (325), Expect = 5.180e-30
Identity = 82/296 (27.70%), Postives = 147/296 (49.66%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFAC--AALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRN-QFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVK-NVITWSAMIAAYGYHGHGRKAIAIF-HLMLNSGVSPNVITFVSLLYA 876
            V + +   G+  N+ + N L+ MY +   L +A  LF  + +++ V+W+VM+GG+ ++  +      FR +++S + P+  T   ++  C ++  L +G+ VH  V K+     N      +L+DMYAKC  +  A+++FD M  + L TW  MI  FA   + D +L LF  M  EG VPD +  V ++ AC   G +   R     + ++ + S  +     MID++ + G  D A  +   M++K +   W +++ A   H     A ++  HL      +P+    +S +YA
Sbjct:  252 VRSWIEDRGLGSNIRLVNGLIDMYVKCGDLEEASNLFEKIQDKNVVSWNVMIGGYTHMSCYKEALGLFRRMMQSNIDPNDVTFLSILPACANLGALDLGKWVHAYVDKNMKSMKNTVALWTSLIDMYAKCGDLAAAKRIFDCMNTKSLATWNAMISGFAMHGHTDTALGLFSRMTSEGFVPDDITFVGVLTACKHAGLLSLGRRYFSSMIQDYKVSPKLPHYGCMIDLFGRAGLFDEAETLVKNMEMKPDGAIWCSLLGACRIHRRIELAESVAKHLFELEPENPSAYVLLSNIYA 547          

HSP 4 Score: 98.2117 bits (243), Expect = 1.670e-20
Identity = 73/274 (26.64%), Postives = 140/274 (51.09%), Query Frame = 1

Query:  271 CRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDL----VTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSG-LIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLG 1077
            C+ ++ LK    +H  ++K GL   +FA + L++  +  +    A + +  M +        T+  +  +  + R A E   +   +++ G+  +     +++N  A+ G +  ARL    +       D +  TA+I  YA  G +D ARE+FD + V++V++W+AMI+ Y   G   +A+A F  M  + V+PNV T +S+L AC+ SG  ++ G  + S + D   + ++I+    ++D+  + G L+EA  L E +  +K+   W  ++G
Sbjct:   40 CKTLQTLKQ---IHSQIIKTGLHNTHFALSKLIEGLSSSESPFLALEYYVHMISSGTEPNEYTFPSIFKSCTKIRGAHEGKQVHAHVLKLGLEHNAFVHTSLINMYAQNGELVNARL----VFDKSSMRDAVSFTALITGYASKGFLDEARELFDEIPVRDVVSWNAMISGYAQSGRVEEAMAFFEEMRRAKVTPNVSTMLSVLSACAQSGSSLQLGNWVRSWIEDR-GLGSNIRLVNGLIDMYVKCGDLEEASNLFEKIQ-DKNVVSWNVMIG 304          

BLAST of ppa025850m vs. Populus trichicarpa v2.0
Match: POPTR_0003s19010.1 ()

HSP 1 Score: 493.812 bits (1270), Expect = 1.366e-139
Identity = 243/553 (43.94%), Postives = 358/553 (64.74%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            VH  V+  G    + V+  L+ MY++   +  A  +F GM  R  V+W+ M+ G+V  GD       F++++  GV P   T+   +  C D+ DL+ G+ VH +V +  L +D     +L+ MY+KCK VD A  +F  ++N+ LV+W  MI  +A+    +E+L  F  M    + PD   MV+++ A A+L    +A+ +H  + R     +V + TA++DMYAKCG I +AR++FD M  ++VITW+AMI  YG HG G+ ++ +F  M    + PN ITF+  L ACSHSGL+EEGL  F  M  ++ +   + HY  MVDLLGRAGRL++A + ++ M ++    ++GA+LGAC+IH N+DL EKA   + +L P++ G++VLL+NIYA A  W  VAK+R +M +  L+K PG + +E+ N+ + F +G   HPQS+KIY+ LE+L  ++  AGYVPDTN + HDV+++VK+ +L +HSEKLAIAFGL+ T+ GTPI I KNLRVCGDCH  TK++S VT R IIVRD++RFH F++G CSCGDYW
Sbjct:  261 VHGYVLRAGFESLVNVSTALVDMYSKCGSVSIARVIFDGMDHRTVVSWNSMIDGYVQSGDAEGAMLIFQKMLDEGVQPTNVTVMGALHACADLGDLERGKFVHKLVDQLKLDSDVSVMNSLISMYSKCKRVDIAADIFKNLRNKTLVSWNAMILGYAQNGCVNEALNAFCEMQSRNIKPDSFTMVSVIPALAELSIPRQAKWIHGLVIRRFLDKNVFVMTALVDMYAKCGAIHTARKLFDMMNARHVITWNAMIDGYGTHGLGKTSVELFKEMKKGTIKPNDITFLCALSACSHSGLVEEGLCFFESMKKDYGIEPTMDHYGAMVDLLGRAGRLNQAWDFIQKMPIKPGITVYGAMLGACKIHKNVDLGEKAAFEIFKLNPDDGGYHVLLANIYATASMWGKVAKVRTIMEKSGLQKTPGCSLVEIGNEVHSFYSGTTSHPQSKKIYSYLETLVDEIRAAGYVPDTNSI-HDVEDDVKVQLLNTHSEKLAIAFGLLNTSTGTPIHIRKNLRVCGDCHNATKYISLVTGREIIVRDMHRFHLFKDGVCSCGDYW 812          

HSP 2 Score: 203.756 bits (517), Expect = 2.823e-52
Identity = 115/387 (29.72%), Postives = 203/387 (52.45%), Query Frame = 1

Query:    1 EVHA---QVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPEN 1152
            EVH    Q++ NG+        KL+ ++ +   L +A  +F  + ++    +  M+ G+       +  + F  +    V P +Y   +++++C D  DLK G+ +H  V+  G   + FA   +V+MYAKC+ ++DA  +FD+M  RDLV W  MI  +A+   A  +L+L   M EEG  PD + +V+I+ A A    +     +H Y+ R  F   V + TA++DMY+KCG +  AR IFD M  + V++W++MI  Y   G    A+ IF  ML+ GV P  +T +  L+AC+  G +E G  +  L+ D+  + +D+     ++ +  +  R+D A ++ +++   K    W A++     +G ++   +A+N+  E+Q  N
Sbjct:   55 EVHQILPQIIKNGLYNETLFQTKLISLFCKYGNLTEASRVFEPIEDKFDALYHTMLKGYAKSSSLDSALSFFSRMKHDSVRPVVYNFTYLLKLCGDNSDLKRGKEIHGSVITSGFSWNLFAMTGVVNMYAKCRQINDAYNMFDRMPERDLVCWNTMISGYAQNGFAKVALMLVLRMSEEGHRPDSITIVSILPAVADTRLLRIGMAVHGYVLRAGFESLVNVSTALVDMYSKCGSVSIARVIFDGMDHRTVVSWNSMIDGYVQSGDAEGAMLIFQKMLDEGVQPTNVTVMGALHACADLGDLERGKFVHKLV-DQLKLDSDVSVMNSLISMYSKCKRVDIAADIFKNLR-NKTLVSWNAMILGYAQNGCVN---EALNAFCEMQSRN 436          

HSP 3 Score: 192.971 bits (489), Expect = 4.983e-49
Identity = 109/365 (29.86%), Postives = 190/365 (52.05%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            E+H  V+ +G   NL     ++ MYA+ R + DAY +F  M ERD V W+ M+ G+   G           +   G  PD  T+  ++    D + L++G  VH  VL+ G  +      ALVDMY+KC  V  AR +FD M +R +V+W  MI  + +  +A+ ++++F  M++EGV P  V ++  ++ACA LG + R + +H  + + +   DV +  ++I MY+KC  +D A +IF  ++ K +++W+AMI  Y  +G   +A+  F  M +  + P+  T VS++ A +   +  +   I  L+   F +  ++   T +VD+  + G +  A +L + M   +    W A++     HG
Sbjct:  159 EIHGSVITSGFSWNLFAMTGVVNMYAKCRQINDAYNMFDRMPERDLVCWNTMISGYAQNGFAKVALMLVLRMSEEGHRPDSITIVSILPAVADTRLLRIGMAVHGYVLRAGFESLVNVSTALVDMYSKCGSVSIARVIFDGMDHRTVVSWNSMIDGYVQSGDAEGAMLIFQKMLDEGVQPTNVTVMGALHACADLGDLERGKFVHKLVDQLKLDSDVSVMNSLISMYSKCKRVDIAADIFKNLRNKTLVSWNAMILGYAQNGCVNEALNAFCEMQSRNIKPDSFTMVSVIPALAELSIPRQAKWIHGLVIRRF-LDKNVFVMTALVDMYAKCGAIHTARKLFDMMNA-RHVITWNAMIDGYGTHG 521          

BLAST of ppa025850m vs. Populus trichicarpa v2.0
Match: POPTR_0013s04190.1 ()

HSP 1 Score: 489.189 bits (1258), Expect = 3.364e-138
Identity = 256/581 (44.06%), Postives = 354/581 (60.93%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHG----LLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEE-------------------------GVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGD 1656
            E+H   V NG++ ++ V N L+ MY++   L  A  LF  M ERD V+WS M+  +     F  G      +  S V P    +  ++ +  D+++ +MG+ +H  V+++     ++     C  L+DMYAKC  +D A  LF     R +V+WT MI  +  C + +E   LF  MIEE                         GV P+++ MV++++ CA  GA+   +  H YI +    +DVIL TA+IDMYAKCG I  A+ +F     +++ TW+ M+A YG HG+G KA+ +F  M   GV PN ITF+  L+ACSH+GL+ EG  +F  M  +F +   ++HY CMVDLLGRAG LDEA +++ESM V  +  +WGA+L AC+IH N ++ E A   LL L+P+N G+ VL+SNIYA A RW DVA MR  +    +KK PG++ IEV+   + F  GD  HP  EKI  ML  + KKL+ AGY+PDT+ VLH++DEE K   L  HSEKLA+AFGLI+TA GTPIR+ KNLR+C DCHT TK +S + +RVIIVRD NRFHHFREG+CSCGD
Sbjct:   31 EIHGFSVKNGLVSDVFVVNALMQMYSECGSLVSARLLFDKMSERDVVSWSTMIRAYSRNKLFIEGLKLIENMHFSNVKPSEVAMISMVNLFSDLENGEMGKAMHGYVIRNSNSEKMVVPLTTC--LIDMYAKCGNLDAAITLFYGFSQRSIVSWTAMIAGYIRCNDLEEGERLFVRMIEENVFPNDITMLSLIISCGFVGAMRDNGVRPNELTMVSLLSLCAVNGALDMGKWFHAYIDKQGVEVDVILKTALIDMYAKCGDISGAQRLFSEAIDRDICTWNVMMAGYGMHGYGEKALKLFTEMETLGVKPNDITFIGALHACSHAGLVVEGKGLFEKMIHDFGLVPKVEHYGCMVDLLGRAGLLDEAYKMIESMPVTPNIAIWGAMLAACKIHKNSNMGELAARELLALEPQNCGYKVLMSNIYAAANRWNDVAGMRKAVKDTGIKKEPGMSSIEVNGLVHDFKMGDTAHPLIEKISEMLAEMSKKLKEAGYLPDTSVVLHNIDEEEKETALNYHSEKLAMAFGLISTAPGTPIRVVKNLRICDDCHTVTKLLSKIYKRVIIVRDRNRFHHFREGSCSCGD 609          

HSP 2 Score: 160.229 bits (404), Expect = 3.579e-39
Identity = 101/329 (30.70%), Postives = 170/329 (51.67%), Query Frame = 1

Query:  238 DIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFS--LDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLML-------------------------NSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQ 1143
            D + +P V++ C  +   +MG+ +H   +K+GL++D F   AL+ MY++C  +  AR LFDKM  RD+V+W+ MI A++  +   E L L + M    V P +VAM+++VN  + L      + +H Y+ RN  S  + V L T +IDMYAKCG +D+A  +F     +++++W+AMIA Y       +   +F  M+                         ++GV PN +T VSLL  C+ +G ++ G + F    D+  V  D+   T ++D+  + G +  A  L  S A+++D   W  ++    +HG     EKA+    E++
Sbjct:    9 DSFIIPSVLKACSQISVARMGKEIHGFSVKNGLVSDVFVVNALMQMYSECGSLVSARLLFDKMSERDVVSWSTMIRAYSRNKLFIEGLKLIENMHFSNVKPSEVAMISMVNLFSDLENGEMGKAMHGYVIRNSNSEKMVVPLTTCLIDMYAKCGNLDAAITLFYGFSQRSIVSWTAMIAGYIRCNDLEEGERLFVRMIEENVFPNDITMLSLIISCGFVGAMRDNGVRPNELTMVSLLSLCAVNGALDMG-KWFHAYIDKQGVEVDVILKTALIDMYAKCGDISGAQRLF-SEAIDRDICTWNVMMAGYGMHG---YGEKALKLFTEME 332          

BLAST of ppa025850m vs. Populus trichicarpa v2.0
Match: POPTR_0002s22590.1 ()

HSP 1 Score: 484.567 bits (1246), Expect = 8.285e-137
Identity = 247/554 (44.58%), Postives = 349/554 (63.00%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVI-LGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +H  VV  G    + ++N L+ MY +      +  +F  M ++++V+WS ++G  +       GF+ FR+++  G  P    +   +   R  ++      V+ +V+++GL  D    +A   M+A+C  V+ AR+LFD + ++DLVTW   I A+ +     E+L L   M+ +G+ PD + ++ ++ AC+ L +   A ++H  I    F   ++ + TA+ID+Y KCG +  AR++FD MQ +N+ITWSAMI+ YG HG GR+A+ +F  M  + V P+ ITFVS+L ACSHSGL+ EG   F+ M  +F V    +HY CMVD+LGRAG+LDEA + +E M V  +  +WGALLGACRIH N+DLAE    +L +L P NAG YV+L NIY   G+ K+   +R LM  R +KK+ G + IE+ NK Y F  GDR HPQ++ IY+ LE L  ++   GY PD NFVLHDVDEE K +MLY HSEKLAI FGL+    G+ IRI KNLRVCGDCHT TKF+S VT R I+VRD +RFHHF+ G CSC DYW
Sbjct:   98 IHQDVVKFGYQSQVFISNSLITMYGKCDKYELSRQVFDEMPDKNAVSWSAIIGACLQDDRCKEGFSLFRQMLSEGSRPSRGAILNAMACVRSHEEADD---VYRVVVENGLDLDQSVQSAAAGMFARCGRVEVARKLFDGIMSKDLVTWATTIEAYVKADMPLEALGLLKQMMLQGIFPDAITLLGVIRACSTLASFQLAHIVHGIITTGFFYNQLLAVETALIDLYVKCGSLTYARKVFDGMQERNIITWSAMISGYGMHGWGREALNLFDQM-KASVKPDHITFVSILSACSHSGLVAEGWECFNSMARDFGVTPRPEHYACMVDILGRAGKLDEACDFIERMPVRPNAAVWGALLGACRIHLNVDLAEMVARALFDLDPHNAGRYVILYNIYTLTGKRKEADSIRTLMKNRGVKKIAGYSVIEIKNKLYAFVAGDRSHPQTDLIYSELERLMDRIRQEGYTPDINFVLHDVDEETKESMLYLHSEKLAIVFGLLNLGPGSVIRIRKNLRVCGDCHTATKFISKVTGREIVVRDAHRFHHFKNGACSCRDYW 647          

HSP 2 Score: 175.252 bits (443), Expect = 1.075e-43
Identity = 98/353 (27.76%), Postives = 181/353 (51.27%), Query Frame = 1

Query:   37 QNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            +NL     L+  YA    +  AY+LF      D   W+VM+ G V+   + +    +++++R G+ PD +T PF+I+ C  ++  + G  +H  V+K G  +  F   +L+ MY KC   + +RQ+FD+M +++ V+W+ +IGA  +     E   LF  M+ EG  P + A   I+NA A + +   A  ++  +  N   LD  + +A   M+A+CG ++ AR++FD +  K+++TW+  I AY       +A+ +   M+  G+ P+ IT + ++ ACS     +    +  ++   F     +   T ++DL  + G L  A ++ + M  E++   W A++    +HG
Sbjct:    8 RNLHFLTNLIAQYASLGSVSYAYSLFSSTPSADLFLWNVMIRGLVDNSHYHHAILLYKQMLRLGIQPDNFTFPFIIKACSCLRHFEFGIRIHQDVVKFGYQSQVFISNSLITMYGKCDKYELSRQVFDEMPDKNAVSWSAIIGACLQDDRCKEGFSLFRQMLSEGSRPSRGA---ILNAMACVRSHEEADDVYRVVVENGLDLDQSVQSAAAGMFARCGRVEVARKLFDGIMSKDLVTWATTIEAYVKADMPLEALGLLKQMMLQGIFPDAITLLGVIRACSTLASFQLAHIVHGIITTGFFYNQLLAVETALIDLYVKCGSLTYARKVFDGMQ-ERNIITWSAMISGYGMHG 356          

HSP 3 Score: 118.627 bits (296), Expect = 1.194e-26
Identity = 77/269 (28.62%), Postives = 131/269 (48.70%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFAC-AALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILG------TAMIDMYAKCGCIDSAREIFDRMQVK-NVITWSAMIAAYGYH 783
            +V+  VV NG+  + +V +    M+A+   +  A  LF G+  +D VTW+  +  +V            ++++  G+ PD  TL  VIR C  +   ++  +VH I+          A   AL+D+Y KC  +  AR++FD MQ R+++TW+ MI  +       E+L LFD M +  V PD +  V+I++AC+     H   +   + C N  + D  +         M+D+  + G +D A +  +RM V+ N   W A++ A   H
Sbjct:  195 DVYRVVVENGLDLDQSVQSAAAGMFARCGRVEVARKLFDGIMSKDLVTWATTIEAYVKADMPLEALGLLKQMMLQGIFPDAITLLGVIRACSTLASFQLAHIVHGIITTGFFYNQLLAVETALIDLYVKCGSLTYARKVFDGMQERNIITWSAMISGYGMHGWGREALNLFDQM-KASVKPDHITFVSILSACS-----HSGLVAEGWECFNSMARDFGVTPRPEHYACMVDILGRAGKLDEACDFIERMPVRPNAAVWGALLGACRIH 457          

BLAST of ppa025850m vs. Populus trichicarpa v2.0
Match: POPTR_0016s13660.1 ()

HSP 1 Score: 483.026 bits (1242), Expect = 2.411e-136
Identity = 243/554 (43.86%), Postives = 353/554 (63.72%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            ++H+ VV  G   +  V   L+ +Y +   + +A+ +F  + +++  +W+  + G+V VG        FR L+  G+ PD ++L  V+  C+   DL+ G  + + + ++G+  + F   ALVD Y KC  ++ AR +FD M  +++V+W+ MI  +A      E+L LF  M+ EG+ PD  AMV ++ +CA+LGA+         I  N+F  + +LGTA+IDMYAKCG +D A E+F  M+ K+ + W+A I+     GH + A+ +F  M  SG+ P+  TFV LL AC+H+GL+EEG R F+ M   + +  +I+HY CMVDLLGRAG LDEA +L++SM +E +  +WGALLG CR+H +  L E  +  L+ L+P ++G+YVLLSNIYA + +W++ AK+R +MS+R +KK+PG +WIEVD   +QF  GD  HP SEKIYA L  L K L+ AGYVP T+ VL D++EE K   +  HSEKLA+AFGLI+TA    I + KNLRVCGDCH   K +S +  R IIVRD NRFH F +G CSC DYW
Sbjct:   31 KMHSLVVKAGCEADAFVKISLINLYTKCGFIDNAFKVFDDIPDKNFASWTATISGYVGVGKCREAIDMFRRLLEMGLRPDSFSLVEVLSACKRTGDLRSGEWIDEYITENGMARNVFVATALVDFYGKCGNMERARSVFDGMLEKNIVSWSSMIQGYASNGLPKEALDLFFKMLNEGLKPDCYAMVGVLCSCARLGALELGDWASNLINGNEFLDNSVLGTALIDMYAKCGRMDRAWEVFRGMRKKDRVVWNAAISGLAMSGHVKDALGLFGQMEKSGIKPDRNTFVGLLCACTHAGLVEEGRRYFNSMECVYTLTPEIEHYGCMVDLLGRAGCLDEAHQLIKSMPMEANAIVWGALLGGCRLHRDTQLVEVVLKKLIALEPWHSGNYVLLSNIYAASHKWEEAAKIRSIMSERGVKKIPGYSWIEVDGVVHQFLVGDTSHPLSEKIYAKLGELAKDLKAAGYVPTTDHVLFDIEEEEKEHFIGCHSEKLAVAFGLISTAPNDKILVVKNLRVCGDCHEAIKHISRIAGREIIVRDNNRFHCFTDGLCSCKDYW 584          

HSP 2 Score: 188.734 bits (478), Expect = 9.397e-48
Identity = 91/295 (30.85%), Postives = 171/295 (57.97%), Query Frame = 1

Query:  220 RSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLM-WDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNI 1101
            + G++PD +T PFV++ C  + D ++G  +H +V+K G  AD F   +L+++Y KC  +D+A ++FD + +++  +WT  I  +       E++ +F  ++E G+ PD  ++V +++AC + G +     +  YI  N  + +V + TA++D Y KCG ++ AR +FD M  KN+++WS+MI  Y  +G  ++A+ +F  MLN G+ P+    V +L +C+  G +E G    +L+  +EF   + +   T ++D+  + GR+D A E+   M  +KD  +W A +    + G++
Sbjct:    3 KEGLSPDSFTFPFVLKACARVLDSELGVKMHSLVVKAGCEADAFVKISLINLYTKCGFIDNAFKVFDDIPDKNFASWTATISGYVGVGKCREAIDMFRRLLEMGLRPDSFSLVEVLSACKRTGDLRSGEWIDEYITENGMARNVFVATALVDFYGKCGNMERARSVFDGMLEKNIVSWSSMIQGYASNGLPKEALDLFFKMLNEGLKPDCYAMVGVLCSCARLGALELGDWASNLINGNEFLDNSVLG--TALIDMYAKCGRMDRAWEVFRGMR-KKDRVVWNAAISGLAMSGHV 294          

HSP 3 Score: 91.6633 bits (226), Expect = 1.563e-18
Identity = 58/211 (27.49%), Postives = 107/211 (50.71%), Query Frame = 1

Query:  517 MIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG----NIDLAEKAVNSLLE 1137
            M +EG+ PD      ++ ACA++        +H  + +     D  +  ++I++Y KCG ID+A ++FD +  KN  +W+A I+ Y   G  R+AI +F  +L  G+ P+  + V +L AC  +G +  G  I   + +    R ++   T +VD  G+ G ++ A  + + M +EK+   W +++     +G     +DL  K +N  L+
Sbjct:    1 MRKEGLSPDSFTFPFVLKACARVLDSELGVKMHSLVVKAGCEADAFVKISLINLYTKCGFIDNAFKVFDDIPDKNFASWTATISGYVGVGKCREAIDMFRRLLEMGLRPDSFSLVEVLSACKRTGDLRSGEWIDEYITENGMAR-NVFVATALVDFYGKCGNMERARSVFDGM-LEKNIVSWSSMIQGYASNGLPKEALDLFFKMLNEGLK 209          

BLAST of ppa025850m vs. Populus trichicarpa v2.0
Match: POPTR_0012s03810.1 ()

HSP 1 Score: 479.174 bits (1232), Expect = 3.481e-135
Identity = 247/612 (40.36%), Postives = 359/612 (58.66%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGER---DSVTWSVMVGGFVNVGDFTNGFATFRELIRSG---VTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEG----------------------------------------------------VVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQF-SLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +H  V + G   N+ V N ++ MY +   L  A  LF  M ER   D V+W+ +V  +V  GD  N    F  + + G   + PD+ +L  V+  C  M     G+ VH I ++ G   D F   ALVDMYAKC +VD+A ++FD+++ +D+V+W  M+  +++    +++L LF+ M EE                                                     V P+   +   + ACA+L A+   R +H YI RN F S  + +   +IDMYAK G ID AR +FD ++ KN ++W++++  YG HG G++A+ +F  M   G+ P+ +T + +LYACSHSG+I++G+  F+ M  EF V    +HY CMVDLLGRAGRL+EA+EL+E M +E    +W ALL  CRIH N++L E A   LLEL  EN G Y LLSNIYA A RWKDVA++R LM    ++K PG +W++    T  F   D+ HPQS++IY +L  L +++++ GYVP+T+F LHDVD+E K+ +L+ HSEKLA+A+G++ +A G PIRITKNLRVCGDCH    ++S +    II+RD +RFHHF++G+CSC  YW
Sbjct:   32 IHGVVFLTGFESNVFVCNAIVAMYGRCGELDCARKLFDEMCERRVYDLVSWNSIVAVYVQRGDCKNALRLFDRMCKLGDIDMRPDVVSLVNVLPACASMGAWLHGKAVHGIAVRSGSFEDLFVGNALVDMYAKCGMVDEASKVFDRIKEKDVVSWNAMVNGYSQIGRFEDALGLFEKMREENIELNVVSWSAVIAGFAQRGLGCETLDVFREMQHGEANDALELFSWIFKQDGLVKPNCFTISCALIACARLAALRLGRQIHAYILRNHFDSAFLYVANCLIDMYAKSGDIDVARFVFDNLKQKNFVSWTSLMTGYGMHGRGKEALEVFDEMRRVGLQPDGVTLLVVLYACSHSGMIDQGIEFFNSMSKEFGVIPGQEHYACMVDLLGRAGRLNEAMELIEGMQMEPSSIVWVALLSGCRIHANVELGEHAAKQLLELNSENDGSYTLLSNIYANARRWKDVARVRSLMKNSGIRKRPGCSWVQGKKGTTTFYVADKTHPQSKQIYEILRGLTQRIKVLGYVPETSFALHDVDDEEKVDLLFEHSEKLALAYGILISAPGAPIRITKNLRVCGDCHNAITYISMIIDHEIILRDSSRFHHFKKGSCSCSGYW 643          

HSP 2 Score: 163.31 bits (412), Expect = 4.228e-40
Identity = 84/242 (34.71%), Postives = 139/242 (57.44%), Query Frame = 1

Query:  220 RSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNR---DLVTWTVMIGAFAECRNADESLVLFDWMIEEGVV---PDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLM 927
            R G TPD YT P V + C D+   ++G  +H +V   G  ++ F C A+V MY +C  +D AR+LFD+M  R   DLV+W  ++  + +  +   +L LFD M + G +   PD V++V ++ ACA +GA    + +H    R+    D+ +G A++DMYAKCG +D A ++FDR++ K+V++W+AM+  Y   G    A+ +F  M    +  NV+++ +++   +  GL  E L +F  M
Sbjct:    3 RLGWTPDNYTFPLVFKACGDLMCCRLGASIHGVVFLTGFESNVFVCNAIVAMYGRCGELDCARKLFDEMCERRVYDLVSWNSIVAVYVQRGDCKNALRLFDRMCKLGDIDMRPDVVSLVNVLPACASMGAWLHGKAVHGIAVRSGSFEDLFVGNALVDMYAKCGMVDEASKVFDRIKEKDVVSWNAMVNGYSQIGRFEDALGLFEKMREENIELNVVSWSAVIAGFAQRGLGCETLDVFREM 244          

BLAST of ppa025850m vs. Populus trichicarpa v2.0
Match: POPTR_0004s05810.1 ()

HSP 1 Score: 478.019 bits (1229), Expect = 7.754e-135
Identity = 235/553 (42.50%), Postives = 345/553 (62.39%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            VH   V   +    T  N LL MYA+  VL  A  +F  M  R  VTW+ ++  +   G        F E+ R GV+PDI+T+  V+  C     L+ G+ VH+ + ++ + ++ F C AL+DMYAKC  ++DA  +F +M  +D+++W  MIG +++    +E+L LF  M+ E + PD   +  I+ ACA L ++ R + +H +I RN F  D  +  A++DMY KCG    AR +FD +  K++ITW+ MIA YG HG G  AI  F+ M  +G+ P+ ++F+S+LYACSHSGL++EG R F++M DE  V+  ++HY C+VDLL R+G+L  A + ++SM +E D  +WGALL  CRIH ++ LAEK    + EL+PEN G+YVLL+N YA A +W++V K+R  + +R LKK PG +WIEV +K + F  G+  HPQ++KI  +L+ L  K++  GY P T + L + D   K   L  HSEKLA+AFG++       IR++KNLRVCGDCH   KF+S    R I++RD NRFHHF++G C C  +W
Sbjct:  227 VHGSGVKACVHWKTTFCNTLLDMYAKCGVLDGAILVFDLMSVRTVVTWTSLIAAYAREGLSDEAIRLFHEMDREGVSPDIFTITTVLHACACNGSLENGKDVHNYIRENDMQSNIFVCNALMDMYAKCGSMEDANSVFLEMPVKDIISWNTMIGGYSKNSLPNEALSLFGDMVLE-MKPDGTTLACILPACASLASLDRGKEVHGHILRNGFFSDQQVANALVDMYVKCGVPVLARLLFDMIPTKDLITWTVMIAGYGMHGFGNNAITTFNEMRQAGIEPDEVSFISILYACSHSGLLDEGWRFFNVMQDECNVKPKLEHYACIVDLLARSGKLAMAYKFIKSMPIEPDATIWGALLSGCRIHHDVKLAEKVAEHVFELEPENTGYYVLLANTYAEAEKWEEVKKLRQKIGRRGLKKNPGCSWIEVKSKVHIFLAGNSSHPQAKKIEVLLKRLRSKMKEEGYFPKTRYALINADSLQKETALCGHSEKLAMAFGILNLPPARTIRVSKNLRVCGDCHEMAKFISKTLGREIVLRDSNRFHHFKDGVCCCRGFW 778          

HSP 2 Score: 197.978 bits (502), Expect = 1.549e-50
Identity = 114/387 (29.46%), Postives = 210/387 (54.26%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAV----NSLLELQPE 1149
            +VH+ +  +G+  +  + +KL++MY     L +   +F  +       W++M+ G+  +GDF    + FR+++  GV  + +T+  V++    +  +K G+ VH  +LK GL + N    +L+  Y K + VD AR+LFD++ NRD+++W  MI  +     +++ + LF  M+  GV  D   MV+I+ ACA  G +   R +H    +            ++DMYAKCG +D A  +FD M V+ V+TW+++IAAY   G   +AI +FH M   GVSP++ T  ++L+AC+ +G +E G  + + +  E  ++++I     ++D+  + G +++A  +   M V KD   W  ++G    +    L  +A+    + +LE++P+
Sbjct:   24 KVHSFICSSGISIDSVLGSKLVFMYVTCGDLREGRLIFDKIRNEKVFLWNLMMNGYTKIGDFKESVSLFRQMLDLGVEVNSHTVSCVLKCFAALGSVKEGKWVHGFLLKLGLGSYNAVVNSLIAFYLKIRRVDVARKLFDELSNRDVISWNSMISGYVANGFSEKGVELFKKMLYLGVDMDLATMVSILQACANCGDVSLGRAVHGSGVKACVHWKTTFCNTLLDMYAKCGVLDGAILVFDLMSVRTVVTWTSLIAAYAREGLSDEAIRLFHEMDREGVSPDIFTITTVLHACACNGSLENGKDVHNYI-RENDMQSNIFVCNALMDMYAKCGSMEDANSVFLEMPV-KDIISWNTMIGG---YSKNSLPNEALSLFGDMVLEMKPD 405          

HSP 3 Score: 186.037 bits (471), Expect = 6.091e-47
Identity = 111/365 (30.41%), Postives = 192/365 (52.60%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFS-LMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            VH  ++  G+     V N L+  Y + R +  A  LF  +  RD ++W+ M+ G+V  G    G   F++++  GV  D+ T+  +++ C +  D+ +GR VH   +K  +      C  L+DMYAKC V+D A  +FD M  R +VTWT +I A+A    +DE++ LF  M  EGV PD   + T+++ACA  G++   + +H YI  N    ++ +  A++DMYAKCG ++ A  +F  M VK++I+W+ MI  Y  +    +A+++F  M+   + P+  T   +L AC+    ++ G  +   ++ + F   +D +    +VD+  + G +     L+  M   KD   W  ++    +HG
Sbjct:  126 VHGFLLKLGLGSYNAVVNSLIAFYLKIRRVDVARKLFDELSNRDVISWNSMISGYVANGFSEKGVELFKKMLYLGVDMDLATMVSILQACANCGDVSLGRAVHGSGVKACVHWKTTFCNTLLDMYAKCGVLDGAILVFDLMSVRTVVTWTSLIAAYAREGLSDEAIRLFHEMDREGVSPDIFTITTVLHACACNGSLENGKDVHNYIRENDMQSNIFVCNALMDMYAKCGSMEDANSVFLEMPVKDIISWNTMIGGYSKNSLPNEALSLFGDMVLE-MKPDGTTLACILPACASLASLDRGKEVHGHILRNGFF--SDQQVANALVDMYVKCG-VPVLARLLFDMIPTKDLITWTVMIAGYGMHG 486          

HSP 4 Score: 124.405 bits (311), Expect = 2.176e-28
Identity = 77/301 (25.58%), Postives = 155/301 (51.50%), Query Frame = 1

Query:  259 VIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYT----CMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPE 1149
            ++++  ++K L+ G+ VH  +   G+  D+   + LV MY  C  + + R +FDK++N  +  W +M+  + +  +  ES+ LF  M++ GV  +   +  ++   A LG++   + +H ++ +        +  ++I  Y K   +D AR++FD +  ++VI+W++MI+ Y  +G   K + +F  ML  GV  ++ T VS+L AC++ G +  G  +         V+A +   T     ++D+  + G LD A+ + + M+V +    W +L+ A    G   L+++A+    E+  E
Sbjct:    9 ILQLSAELKSLQDGKKVHSFICSSGISIDSVLGSKLVFMYVTCGDLREGRLIFDKIRNEKVFLWNLMMNGYTKIGDFKESVSLFRQMLDLGVEVNSHTVSCVLKCFAALGSVKEGKWVHGFLLKLGLGSYNAVVNSLIAFYLKIRRVDVARKLFDELSNRDVISWNSMISGYVANGFSEKGVELFKKMLYLGVDMDLATMVSILQACANCGDVSLGRAVHG-----SGVKACVHWKTTFCNTLLDMYAKCGVLDGAILVFDLMSV-RTVVTWTSLIAAYAREG---LSDEAIRLFHEMDRE 300          

HSP 5 Score: 73.559 bits (179), Expect = 4.406e-13
Identity = 48/192 (25.00%), Postives = 98/192 (51.04%), Query Frame = 1

Query:  559 TIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLL----GRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAV 1122
            +I+   A+L ++   + +H +IC +  S+D +LG+ ++ MY  CG +   R IFD+++ + V  W+ M+  Y   G  ++++++F  ML+ GV  N  T   +L   +  G ++EG  +       F ++  +  Y  +V+ L     +  R+D A +L + ++  +D   W +++     +G    +EK V
Sbjct:    8 SILQLSAELKSLQDGKKVHSFICSSGISIDSVLGSKLVFMYVTCGDLREGRLIFDKIRNEKVFLWNLMMNGYTKIGDFKESVSLFRQMLDLGVEVNSHTVSCVLKCFAALGSVKEGKWVHG-----FLLKLGLGSYNAVVNSLIAFYLKIRRVDVARKLFDELS-NRDVISWNSMISGYVANG---FSEKGV 190          

HSP 6 Score: 57.3806 bits (137), Expect = 3.267e-8
Identity = 30/91 (32.97%), Postives = 48/91 (52.75%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVC 273
            EVH  ++ NG   +  VAN L+ MY +  V   A  LF  +  +D +TW+VM+ G+   G   N   TF E+ ++G+ PD  +   ++  C
Sbjct:  427 EVHGHILRNGFFSDQQVANALVDMYVKCGVPVLARLLFDMIPTKDLITWTVMIAGYGMHGFGNNAITTFNEMRQAGIEPDEVSFISILYAC 517          

BLAST of ppa025850m vs. Populus trichicarpa v2.0
Match: POPTR_0001s32930.1 ()

HSP 1 Score: 476.093 bits (1224), Expect = 2.947e-134
Identity = 246/564 (43.62%), Postives = 351/564 (62.23%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELI---------RSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVP-DKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            + H Q +V G   +L V++ L+ MY++   L +A  LF  +  R+ VTW+ ++ G+V   D       F+E +           G + D   +  V+  C  + +  +   VH + +K GL         L+D YAKC  V  +R++FD M  +D+V+W  MI  +A+   + ++  +F  M++ G    ++V + T++ ACA  GA+     LH  + +  +  +VI+ T++IDMY KCG  + AR  FD M+ KNV +W+AMIA YG HG  R+A+ +F+ M+ +GV PN ITF+S+L ACSH+G +EEG R F+ M  E+ V   ++HY CMVDLLGRAG + EA  L++SM V +D  LWG+LL ACRIH +++LAE +   L +L P N G+YVLL+NIYA AGRWKDV +MR L+  R L K PG + +E+  + + F  GD+ HPQ EKIY  LE L  KL+ AGYVP+   VLHDVDEE K  ++  HSEKLA+AFG++ +  G+ I + KNLRVCGDCHT  K +S +  R IIVRD  RFHHF++G CSCGDYW
Sbjct:  103 QAHQQALVFGFESDLFVSSALIDMYSKCGKLSNARVLFDEIPRRNIVTWTSLITGYVQNDDAHEALMVFKEFLFEKSEGNGEEVGTSVDSVAMISVLSACSRVSNKAVSEGVHGVAIKVGLDKVMGVENTLLDAYAKCGEVSLSRKVFDDMAEKDVVSWNSMIAVYAQNGLSTDAFEVFHGMLKAGGGKYNEVTLSTLLLACAHEGALRVGMCLHDQVIKMGYVNNVIMATSIIDMYCKCGQAEMARNAFDGMKEKNVRSWTAMIAGYGMHGFAREALDVFYQMIWAGVKPNYITFISVLAACSHAGFLEEGWRWFNAMSHEYNVEPGVEHYGCMVDLLGRAGYIKEAYNLIKSMKVRRDFVLWGSLLAACRIHKDVELAEISARELFKLDPSNCGYYVLLANIYADAGRWKDVERMRILVKDRGLVKPPGYSLVELKGRVHVFLVGDKEHPQHEKIYKYLEELSVKLQEAGYVPNMASVLHDVDEEEKEMIVRVHSEKLAVAFGVMNSIPGSTIHVIKNLRVCGDCHTVIKLISKIVSREIIVRDAKRFHHFKDGLCSCGDYW 666          

HSP 2 Score: 167.162 bits (422), Expect = 2.928e-41
Identity = 98/333 (29.43%), Postives = 174/333 (52.25%), Query Frame = 1

Query:  133 DSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMI---------EEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILG--TAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSP-NVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            D  +W+ ++      GD       F  + +  + P+  T P  I+ C  + DL  G+  H   L  G  +D F  +AL+DMY+KC  + +AR LFD++  R++VTWT +I  + +  +A E+L++F   +         E G   D VAM+++++AC+++     +  +H    +    LD ++G    ++D YAKCG +  +R++FD M  K+V++W++MIA Y  +G    A  +FH ML +G    N +T  +LL AC+H G +  G+ +   +     V  ++   T ++D+  + G+ + A    + M  EK+ R W A++    +HG
Sbjct:   46 DVYSWNSLIAELARGGDSCESLRAFSWMRKLDIKPNRSTFPCAIKSCSALFDLNSGKQAHQQALVFGFESDLFVSSALIDMYSKCGKLSNARVLFDEIPRRNIVTWTSLITGYVQNDDAHEALMVFKEFLFEKSEGNGEEVGTSVDSVAMISVLSACSRVSNKAVSEGVHGVAIK--VGLDKVMGVENTLLDAYAKCGEVSLSRKVFDDMAEKDVVSWNSMIAVYAQNGLSTDAFEVFHGMLKAGGGKYNEVTLSTLLLACAHEGALRVGMCLHDQVIKMGYVN-NVIMATSIIDMYCKCGQAEMARNAFDGMK-EKNVRSWTAMIAGYGMHG 374          

BLAST of ppa025850m vs. Populus trichicarpa v2.0
Match: POPTR_0013s14820.1 ()

HSP 1 Score: 475.707 bits (1223), Expect = 3.848e-134
Identity = 238/553 (43.04%), Postives = 348/553 (62.93%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            VH Q+  +G   ++ V N L+ +YA+   +  A A+FG + +R  V+W+ ++ G+   G        F E+ ++ V PD   L  V+R   D++DL+ G+ +H  V+K GL  +     +L  +YAKC  V  AR  F++++N  L+ W  MI  + +   A+E++ LF  M  + + PD + + + + ACA++G++  AR +  YI  ++F  DVI+ T++ID YAKCG +D AR +FDR+  K+V+ WSAM+  YG HG GR++I +FH M  +GVSPN +TFV LL AC +SGL+EEG  +F  M D + +    +HY C+VDLLGRAG LD A   V +M +E    +WGALL AC+IH ++ L E A   L  L P N GHYV LSN+YA +  W  VAK+R LM ++ L K  G + IE++ K   F  GD+ HP+S++I+  +E L ++L+ AG+VP T  VLHD++ E     L +HSE+LAIA+GLI+T  GT +RITKNLR C +CH   K +S +  R I+VRD  RFHHF++G CSCGDYW
Sbjct:   36 VHGQIFRHGFESDVFVQNGLVALYAKCGEIVRANAVFGRLVDRTIVSWTSIISGYAQNGQPIEALRIFSEMRKTNVRPDWIALVSVLRAYTDVEDLEHGKSIHGCVIKMGLECEFDLLISLTSLYAKCGHVMVARLFFNQVENPSLIFWNAMISGYVKNGYAEEAIELFRLMKSKNIRPDSITVTSSIAACAQIGSLELARWMDEYISMSEFRNDVIVNTSLIDTYAKCGSVDMARFVFDRIPDKDVVVWSAMMVGYGLHGQGRESIILFHAMRQAGVSPNDVTFVGLLTACKNSGLVEEGWDLFHRMRD-YGIEPRHQHYACVVDLLGRAGHLDRAYNFVMNMPIEPGVSVWGALLSACKIHRHVTLGEYAAERLFSLDPYNTGHYVQLSNLYASSCLWDCVAKVRVLMREKGLTKHLGYSVIEINGKLQAFQAGDKTHPRSKEIFEEVEDLERRLKEAGFVPHTESVLHDLNYEETEETLCNHSERLAIAYGLISTPPGTTLRITKNLRACDNCHAAIKLISKLVSREIVVRDACRFHHFKDGACSCGDYW 587          

HSP 2 Score: 150.599 bits (379), Expect = 2.836e-36
Identity = 86/293 (29.35%), Postives = 156/293 (53.24%), Query Frame = 1

Query:  229 VTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEF----AVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            V+PD ++ P V++ C  +  L+MGR VH  + +HG  +D F    LV +YAKC  +  A  +F ++ +R +V+WT +I  +A+     E+L +F  M +  V PD +A+V+++ A   +  +   + +H  + +     +  L  ++  +YAKCG +  AR  F++++  ++I W+AMI+ Y  +G+  +AI +F LM +  + P+ IT  S + AC+  G +E      +   DE+      R D+   T ++D   + G +D A   V     +KD  +W A++    +HG
Sbjct:   10 VSPDGFSFPCVLKACSALPALEMGRRVHGQIFRHGFESDVFVQNGLVALYAKCGEIVRANAVFGRLVDRTIVSWTSIISGYAQNGQPIEALRIFSEMRKTNVRPDWIALVSVLRAYTDVEDLEHGKSIHGCVIKMGLECEFDLLISLTSLYAKCGHVMVARLFFNQVENPSLIFWNAMISGYVKNGYAEEAIELFRLMKSKNIRPDSITVTSSIAACAQIGSLE-----LARWMDEYISMSEFRNDVIVNTSLIDTYAKCGSVDMA-RFVFDRIPDKDVVVWSAMMVGYGLHG 296          

BLAST of ppa025850m vs. GDR
Match: MDP0000275151 (MDP0000275151)

HSP 1 Score: 981.86 bits (2537), Expect = 0.000e+0
Identity = 469/554 (84.66%), Postives = 516/554 (93.14%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +VHAQV  NGMLQNLTVANKLLYMY Q+RVLG+AYALFGGM E+D+V+WSVMVGGFV  GD+TNGFATFRELIR GV+PD YTLPFVIRVCRD+ DL+MGRLVH IVLKHGL++DNF CAALVDMYAKC+V+DDARQLFD MQ+RDLV+WTVMIGA AECRNADE+LVLFD M++EGV+PDKVAM T+VNACAKLGAMH+ARL+  YICRN+FSLDVILGTAMIDMYAKCGC+DSAREIFDRM VKNVITWSAMIAAYGYHGHGRKAI IF +ML+SGVSPN ITF+SLLYACSHSGLIEEGLR FS MWDE+AVRAD+KHYTCMVDLLGRAG+LDEAL+LVES AVEKDE LWGA LGACRIHGN+DLA+K VNSLLELQPENAGHYVLLSNIYAR GRWK+++KMRDLM+QR+LKK+PGLTWIEVDNK YQFS GDR HP SEKIYAM++SL +KLELAGYVPDTNFVLHDVDEEVKL ML SHSEKLAIAFGL+ATADGTPIRITKNLRVCGDCH+F KFVSA+TQRVIIVRD NRFHHF+EG CSCGDYW
Sbjct: 1075 QVHAQVTANGMLQNLTVANKLLYMYTQQRVLGEAYALFGGMEEKDAVSWSVMVGGFVKAGDYTNGFATFRELIRGGVSPDNYTLPFVIRVCRDLMDLQMGRLVHGIVLKHGLVSDNFVCAALVDMYAKCRVIDDARQLFDNMQSRDLVSWTVMIGACAECRNADEALVLFDRMVDEGVLPDKVAMATVVNACAKLGAMHKARLVDDYICRNKFSLDVILGTAMIDMYAKCGCVDSAREIFDRMHVKNVITWSAMIAAYGYHGHGRKAIDIFEMMLSSGVSPNAITFISLLYACSHSGLIEEGLRFFSSMWDEYAVRADVKHYTCMVDLLGRAGKLDEALKLVESTAVEKDEGLWGAFLGACRIHGNVDLAKKVVNSLLELQPENAGHYVLLSNIYAREGRWKEMSKMRDLMTQRKLKKIPGLTWIEVDNKIYQFSVGDRSHPWSEKIYAMIKSLNEKLELAGYVPDTNFVLHDVDEEVKLGMLSSHSEKLAIAFGLLATADGTPIRITKNLRVCGDCHSFIKFVSAITQRVIIVRDANRFHHFKEGACSCGDYW 1628          

HSP 2 Score: 145.976 bits (367), Expect = 9.452e-35
Identity = 85/299 (28.43%), Postives = 155/299 (51.84%), Query Frame = 1

Query:  256 FVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVR----ADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGN----IDLAEKAVNS 1128
            F + V    +++   R VH  V  +G+L +      L+ MY + +V+ +A  LF  M+ +D V+W+VM+G F +  +       F  +I  GV PD   +  ++  C  L  +   RL+H  + ++    D  +  A++DMYAKC  ID AR++FD MQ +++++W+ MI A     +  +A+ +F  M++ GV P+ +   +++ AC+  G + +   +     D++  R     D+   T M+D+  + G +D A E+ + M V K+   W A++ A   HG+    ID+ E  ++S
Sbjct: 1059 FYVNVLLSSRNIFHIRQVHAQVTANGMLQNLTVANKLLYMYTQQRVLGEAYALFGGMEEKDAVSWSVMVGGFVKAGDYTNGFATFRELIRGGVSPDNYTLPFVIRVCRDLMDLQMGRLVHGIVLKHGLVSDNFVCAALVDMYAKCRVIDDARQLFDNMQSRDLVSWTVMIGACAECRNADEALVLFDRMVDEGVLPDKVAMATVVNACAKLGAMHKARLV-----DDYICRNKFSLDVILGTAMIDMYAKCGCVDSAREIFDRMHV-KNVITWSAMIAAYGYHGHGRKAIDIFEMMLSS 1351          

BLAST of ppa025850m vs. GDR
Match: MDP0000548396 (MDP0000548396)

HSP 1 Score: 502.671 bits (1293), Expect = 3.978e-142
Identity = 247/554 (44.58%), Postives = 364/554 (65.70%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKH-GLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            VH +VVV+G+  ++ V N LL MY++   +  A  +F  M ERD  +W+ M+ G V  GD   G   F E+ ++G+  D  +L  ++  C ++  LK G+ VH  V+++ G + + F   +L++MY KCK    +R+LFD ++ +D V+W  MI  + +  +A ESL LF  M+ EG   D+V + +++ AC ++ A+     +H  I +  F  ++ +GTA+IDMY+KCG +  +R +FD M VKN+++WSAM++ YG HG G +AI+ +H ++ +  +P+     S+L ACSH+GL+ EG  IF+ M  E+ V+  + HY+C+VDLLGRAGR+DEA EL++SM VE    +W ALL ACR+H N+ LAE A  ++ E+ P+  G Y+ LSNIYA   RW DV ++R ++ ++ LKK PG +++E+D   ++F  GD+ HPQ+  IYA L  L  +L+ AGY PDT  V +DV+E+VK  ML+ HSE+LAIAF LI T  GT IRITKNLRVC DCHT TK +S +  R II+RD++RFHHFR G CSCGDYW
Sbjct:  158 VHGEVVVSGLESDVYVGNALLAMYSKFGDMRLAGMVFDRMPERDLTSWNTMISGCVKNGDPREGLMIFEEMGKAGLRADGTSLLGILSGCAELMALKEGKAVHAYVVRNSGEIHNEFLTNSLIEMYCKCKSAAYSRRLFDGVKLKDTVSWNSMISGYEQNGDAFESLRLFCQMVMEGAEVDEVTVTSVLGACDQISALQFGMSVHSCIVKKGFGGNIFVGTALIDMYSKCGSLSCSRCVFDEMPVKNLVSWSAMVSGYGAHGRGEEAISCYHELVANNFTPDEGVLTSVLSACSHAGLVNEGKDIFNRMTVEYNVKPGLAHYSCLVDLLGRAGRIDEAYELIKSMQVEPSSDIWAALLSACRLHKNVKLAEIAAQNVFEMNPKGVGSYICLSNIYASEKRWDDVERVRAVVRRKGLKKPPGCSFVELDKMVHRFLVGDKSHPQTRDIYAKLTDLNLQLKEAGYKPDTASVFYDVEEKVKEKMLWDHSERLAIAFALINTQPGTTIRITKNLRVCNDCHTVTKMISMLMNREIIMRDIHRFHHFRHGVCSCGDYW 711          

BLAST of ppa025850m vs. GDR
Match: MDP0000764434 (MDP0000764434)

HSP 1 Score: 502.671 bits (1293), Expect = 3.978e-142
Identity = 247/554 (44.58%), Postives = 364/554 (65.70%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKH-GLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            VH +VVV+G+  ++ V N LL MY++   +  A  +F  M ERD  +W+ M+ G V  GD   G   F E+ ++G+  D  +L  ++  C ++  LK G+ VH  V+++ G + + F   +L++MY KCK    +R+LFD ++ +D V+W  MI  + +  +A ESL LF  M+ EG   D+V + +++ AC ++ A+     +H  I +  F  ++ +GTA+IDMY+KCG +  +R +FD M VKN+++WSAM++ YG HG G +AI+ +H ++ +  +P+     S+L ACSH+GL+ EG  IF+ M  E+ V+  + HY+C+VDLLGRAGR+DEA EL++SM VE    +W ALL ACR+H N+ LAE A  ++ E+ P+  G Y+ LSNIYA   RW DV ++R ++ ++ LKK PG +++E+D   ++F  GD+ HPQ+  IYA L  L  +L+ AGY PDT  V +DV+E+VK  ML+ HSE+LAIAF LI T  GT IRITKNLRVC DCHT TK +S +  R II+RD++RFHHFR G CSCGDYW
Sbjct:  158 VHGEVVVSGLESDVYVGNALLAMYSKFGDMRLAGMVFDRMPERDLTSWNTMISGCVKNGDPREGLMIFEEMGKAGLRADGTSLLGILSGCAELMALKEGKAVHAYVVRNSGEIHNEFLTNSLIEMYCKCKSAAYSRRLFDGVKLKDTVSWNSMISGYEQNGDAFESLRLFCQMVMEGAEVDEVTVTSVLGACDQISALQFGMSVHSCIVKKGFGGNIFVGTALIDMYSKCGSLSCSRCVFDEMPVKNLVSWSAMVSGYGAHGRGEEAISCYHELVANNFTPDEGVLTSVLSACSHAGLVNEGKDIFNRMTVEYNVKPGLAHYSCLVDLLGRAGRIDEAYELIKSMQVEPSSDIWAALLSACRLHKNVKLAEIAAQNVFEMNPKGVGSYICLSNIYASEKRWDDVERVRAVVRRKGLKKPPGCSFVELDKMVHRFLVGDKSHPQTRDIYAKLTDLNLQLKEAGYKPDTASVFYDVEEKVKEKMLWDHSERLAIAFALINTQPGTTIRITKNLRVCNDCHTVTKMISMLMNREIIMRDIHRFHHFRHGVCSCGDYW 711          

BLAST of ppa025850m vs. GDR
Match: MDP0000144150 (MDP0000144150)

HSP 1 Score: 497.664 bits (1280), Expect = 1.280e-140
Identity = 248/555 (44.68%), Postives = 354/555 (63.78%), Query Frame = 1

Query:    4 VHAQVVVNGMLQN--LTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +H  VV NG  +   + V   ++ MY +   +  A  LF G+G+++ V+W+ M+ G+++  D   G   F  ++  G  P+  T+  +I     +  +++G+ +H  +L+HG +       ALVDMY KC     AR +FD +  +D++ W+ +I A+A     +E+  LF  M   G  P++V MV++++ CA++GA+   + +H YI + +  +DVIL TA++DMYAKCG ID A  +FD    +++  W+AM+  +  HG G++A+ +F  M    V PN ITF+ +L+ACSH+GL+ EG   F  M     +   ++HY CMVDLLGRAG+L EA EL++SM ++ +  +WGALL AC+IH N DLAE A   LL L+P N G+ +L+SNIYA + RW DVA +R  M  R  KK PGL+ IEV+   + F  GDR HPQ+ KIY ML  + KKL+ AGY P+T+ VL ++DEE K   +  HSEKLA+AFGLIA A GTPIR+ KNLRVC DCHT TK +S +  RVIIVRD NRFHHFREG+CSCGDYW
Sbjct:  249 MHVHVVRNGKHEKFGVPVTTAMIDMYVKCGNVTYARRLFDGLGQKNIVSWTAMIAGYIHCRDLHEGAKLFNRMLAEGSFPNEITMLSLIIESGSVGAMELGKWLHAYILRHGFVMSLPLATALVDMYGKCGETRYARAVFDSVDXKDVMIWSALISAYAHANCTNEASQLFARMKNSGARPNQVTMVSLISLCAEVGALDLGKWVHSYINQQRVEVDVILRTALVDMYAKCGEIDMALRLFDEATNRDICMWNAMMTGFSMHGCGKQALELFEQMNREAVEPNDITFIGVLHACSHAGLVAEGENFFEKMVRVHGLAPKVEHYGCMVDLLGRAGKLHEAHELIKSMPIQPNTIVWGALLAACKIHKNPDLAEVASRQLLXLEPRNCGYNILMSNIYAASNRWNDVAGVRKAMKDRGTKKEPGLSSIEVNGSVHDFVMGDRAHPQAGKIYEMLAEMIKKLKEAGYAPNTSVVLQNIDEEEKETAVNYHSEKLAMAFGLIAXAAGTPIRVVKNLRVCDDCHTATKLLSKIYGRVIIVRDRNRFHHFREGSCSCGDYW 803          

HSP 2 Score: 213.386 bits (542), Expect = 4.821e-55
Identity = 121/368 (32.88%), Postives = 202/368 (54.89%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNF---ACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            E H   + +G+  ++ V N L+ MY++   +  A  LF  M +RD V+WS M+   V    F       RE+ R  V P    +  ++ +   + D++MG+ +H  V+++G   + F      A++DMY KC  V  AR+LFD +  +++V+WT MI  +  CR+  E   LF+ M+ EG  P+++ M++++     +GAM   + LH YI R+ F + + L TA++DMY KCG    AR +FD +  K+V+ WSA+I+AY +     +A  +F  M NSG  PN +T VSL+  C+  G ++ G  + S + ++  V  D+   T +VD+  + G +D AL L +  A  +D  +W A++    +HG
Sbjct:  147 ETHGFALKSGLDGDVFVRNALIQMYSESGNVVLARLLFDKMTDRDVVSWSTMIRSCVRNRLFGEALDLIREMHRVRVKPSEIAMISMVNLFAGVADIEMGKAMHVHVVRNG-KHEKFGVPVTTAMIDMYVKCGNVTYARRLFDGLGQKNIVSWTAMIAGYIHCRDLHEGAKLFNRMLAEGSFPNEITMLSLIIESGSVGAMELGKWLHAYILRHGFVMSLPLATALVDMYGKCGETRYARAVFDSVDXKDVMIWSALISAYAHANCTNEASQLFARMKNSGARPNQVTMVSLISLCAEVGALDLGKWVHSYI-NQQRVEVDVILRTALVDMYAKCGEIDMALRLFDE-ATNRDICMWNAMMTGFSMHG 511          

HSP 3 Score: 154.451 bits (389), Expect = 2.657e-37
Identity = 97/294 (32.99%), Postives = 154/294 (52.38%), Query Frame = 1

Query:  205 FRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRN--QFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGA 1080
            + ++ R     D +T+P V++ C       +G+  H   LK GL  D F   AL+ MY++   V  AR LFDKM +RD+V+W+ MI +    R   E+L L   M    V P ++AM+++VN  A +  +   + +H ++ RN       V + TAMIDMY KCG +  AR +FD +  KN+++W+AMIA Y +     +   +F+ ML  G  PN IT +SL+      G +E G  + + +     V + +   T +VD+ G+ G    A  + +S+   KD  +W AL+ A
Sbjct:  114 YAQMRRMDTQVDSFTIPSVLKACGQSSFAXLGKETHGFALKSGLDGDVFVRNALIQMYSESGNVVLARLLFDKMTDRDVVSWSTMIRSCVRNRLFGEALDLIREMHRVRVKPSEIAMISMVNLFAGVADIEMGKAMHVHVVRNGKHEKFGVPVTTAMIDMYVKCGNVTYARRLFDGLGQKNIVSWTAMIAGYIHCRDLHEGAKLFNRMLAEGSFPNEITMLSLIIESGSVGAMELGKWLHAYILRHGFVMS-LPLATALVDMYGKCGETRYARAVFDSVD-XKDVMIWSALISA 405          

BLAST of ppa025850m vs. GDR
Match: MDP0000144202 (MDP0000144202)

HSP 1 Score: 493.041 bits (1268), Expect = 3.152e-139
Identity = 253/554 (45.67%), Postives = 356/554 (64.26%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +VH  V+  G   ++ V N L+ +Y +   +  A  +F  + +R+ V+WS M+G +   G F +G+  F  ++   +TP+  ++   +       D      V  +V  +GL  D     A + MYA+C  +D A+  FD++ ++DLV+W+ MI A+A+     E++ LF  M E+GV+PD VA++++V AC+ L +  +AR +H  + R+ FS  + L T+++D+Y KCG +  AR +FD MQ +N+I+WS MI+ YG HGHGR+A+ +FH M  + + P+ I F+S+L ACSH GLI EG   F+ M  +F V    +HY CMVDLLGR+GRLDEA E +E M V  D  +WG+LLGACR H N +LA+ A   L EL  EN G YVL+SNIYA +G+ KD   +RDLM QR ++KV G T IE+ NK Y F  GD  H QS+ IY  LE L  ++   GY PD NFVLHDV++E+K  MLY+HSEKLAI FGL+     + IRI KNLRVCGDCHT +KF+S VT R I++RD +RFHHF++G CSCGDYW
Sbjct:  120 KVHGDVIECGYALDVFVGNSLIGLYGKFGQVETARKVFYKIPQRNVVSWSSMIGVYAQNGCFEDGWXXFLMMLNEKITPNRASVLNAMACVFQENDAGE---VCGVVTDNGLDLDRSVQNAAMQMYARCGRMDIAQVFFDRISDKDLVSWSSMIEAYAQADLPLEAVELFKEMXEQGVLPDLVALLSVVXACSSLASFQQARWIHGLVIRSFFSNCIALETSIVDLYVKCGSLMYARRVFDEMQERNIISWSTMISGYGMHGHGREAVNLFHQM-KALIIPDHIAFLSVLSACSHGGLIAEGWDCFNSMSRDFHVTPRPEHYACMVDLLGRSGRLDEAFEFIERMPVRPDAGVWGSLLGACRXHSNTELAKIAAGHLFELDAENPGRYVLMSNIYASSGKQKDADDIRDLMKQRGVRKVAGHTSIEIKNKVYTFVAGDHSHAQSDLIYMELEKLINRIRQEGYXPDLNFVLHDVEDEMKEKMLYAHSEKLAIVFGLLNLGPESVIRIKKNLRVCGDCHTASKFISKVTGREIVMRDAHRFHHFKDGACSCGDYW 669          

HSP 2 Score: 169.474 bits (428), Expect = 7.983e-42
Identity = 112/376 (29.79%), Postives = 194/376 (51.60%), Query Frame = 1

Query:    4 VHAQVV-VNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSV-TWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVN 1125
            VHA V+  + +  NL +   L+  YA       AY LF  +     V  W+VM+ GFV+ G +    A + ++   GV PD +T PFVI+ C   ++ ++G+ VH  V++ G   D F   +L+ +Y K   V+ AR++F K+  R++V+W+ MIG +A+    ++    F  M+ E + P++    +++NA A +   + A  +   +  N   LD  +  A + MYA+CG +D A+  FDR+  K++++WS+MI AY       +A+ +F  M   GV P+++  +S++ ACS     ++   I  L+   F     I   T +VDL  + G L  A  + + M  E++   W  ++    +HG+     +AVN
Sbjct:   18 VHAAVLRTHDLHLNLFLCTNLIAHYASLGSPSHAYILFSAVSSASDVFLWNVMIRGFVDNGLYNKAIALYSKMWDVGVRPDHFTFPFVIKACGFARNWRLGKKVHGDVIECGYALDVFVGNSLIGLYGKFGQVETARKVFYKIPQRNVVSWSSMIGVYAQNGCFEDGWXXFLMMLNEKITPNR---ASVLNAMACVFQENDAGEVCGVVTDNGLDLDRSVQNAAMQMYARCGRMDIAQVFFDRISDKDLVSWSSMIEAYAQADLPLEAVELFKEMXEQGVLPDLVALLSVVXACSSLASFQQARWIHGLVIRSFFSNC-IALETSIVDLYVKCGSLMYARRVFDEMQ-ERNIISWSTMISGYGMHGH---GREAVN 385          

BLAST of ppa025850m vs. GDR
Match: MDP0000436155 (MDP0000436155)

HSP 1 Score: 492.271 bits (1266), Expect = 5.376e-139
Identity = 240/555 (43.24%), Postives = 357/555 (64.32%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACA-ALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIE-EGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +H  V+ +G+  +L V N L+ MY++   LG A  +F  M  RD V+W+ +V  +    D       F  +   G+ PD  TL  +  +   + D +  + VH  +L+     ++     A+VDMYAK   +D AR +F+ +  +D V+W  +I  +A+  +A E++ ++  M + + ++P+    V+I+ A   +GA+ +   +H  + +N+   DV +GT +IDMY KCG +D A  +F ++  K+ + W+A+I+ +G HGHG KA+ +F+ ML+ GV P+ +TFVSL+ ACSHSGL+ EG   F  M +++ ++  +KHY CMVDLLGRAG L++A   +E+M+V  D  +WGALLGACRIHGN+++ + A   L E+  EN G+YVLLSNIYA  G+W+ V K+R L   R L K PG + IEV+N    F T ++ HP+ E+IY  L  L  +++  GY+PD +FVL DV+++ K  +L SHSE+LAIAFG+I+TA  TPIRI KNLRVCGDCH  TKF+S +T+R IIVRD NRFHHF++GTCSCGDYW
Sbjct:  289 IHLYVIKHGLDFDLFVCNALINMYSKFGSLGHARRVFDQMEVRDLVSWNSIVAAYEQNNDPITALELFNAMQLRGIQPDFLTLVSLASILAQLSDSEKSKSVHGFILRRDWFVEDVVIGNAVVDMYAKLGAIDSARTVFEGLPTKDAVSWNTLITGYAQNGHASEAIEVYRMMQQCKEIIPNHGTWVSILPAYTHVGALQQGMKVHGRVIKNRVYSDVFVGTCLIDMYGKCGRLDDALCLFSQVPRKSSVPWNALISCHGVHGHGEKALQLFNDMLDEGVKPDHVTFVSLMAACSHSGLVNEGQSFFHTMQEQYGIKPSLKHYGCMVDLLGRAGHLNKAYSFIENMSVRPDASVWGALLGACRIHGNVEMGKIASERLFEVDSENVGYYVLLSNIYANFGKWEGVEKVRSLARNRGLTKTPGWSSIEVNNNVDVFYTANQSHPKCEEIYEKLRDLTARMKSLGYIPDLSFVLQDVEDDEKEHILNSHSERLAIAFGIISTAPKTPIRIFKNLRVCGDCHNATKFISVITEREIIVRDSNRFHHFKDGTCSCGDYW 843          

HSP 2 Score: 192.971 bits (489), Expect = 6.742e-49
Identity = 122/421 (28.98%), Postives = 214/421 (50.83%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFREL-IRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRI--FSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQP--ENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRL 1248
            ++HA +VV G  QN+ +A KL+  YA    L  +   F  +  +D  TW+ MV  +V  G F      F +  + S + PD YT P V++ C ++ D   G+ +H  VLK G   D F  A+L+ MY++   +  AR+LFD M  RD  +W  MI  F +  NA ++L +   M  EG+  D+V + +++ ACA+   +   RL+H Y+ ++    D+ +  A+I+MY+K G +  AR +FD+M+V+++++W++++AAY  +     A+ +F+ M   G+ P+ +T VSL    +     E+   +  F L  D F    D+     +VD+  + G +D A  + E +   KD   W  L+     +G+   A +    + + +    N G +V +   Y   G  +   K+   + + R+
Sbjct:   88 QLHAYLVVLGKSQNIFLAAKLVNRYAYLGDLSFSRRTFDSIPRKDVYTWNSMVSAYVRSGRFREALDCFSQFSLTSDLHPDFYTFPPVLKACENLVD---GKKIHCRVLKLGFEWDVFVAASLIHMYSRFGFIYVARKLFDDMPIRDTGSWNAMISGFCQNANAADALDVLVEMRMEGIKMDRVTVASLLTACAQSNDISSGRLIHLYVIKHGLDFDLFVCNALINMYSKFGSLGHARRVFDQMEVRDLVSWNSIVAAYEQNNDPITALELFNAMQLRGIQPDFLTLVSLASILAQLSDSEKSKSVHGFILRRDWFV--EDVVIGNAVVDMYAKLGAIDSARTVFEGLPT-KDAVSWNTLITGYAQNGHASEAIEVYRMMQQCKEIIPNHGTWVSILPAYTHVGALQQGMKVHGRVIKNRV 502          

HSP 3 Score: 67.781 bits (164), Expect = 3.271e-11
Identity = 51/193 (26.42%), Postives = 93/193 (48.19%), Query Frame = 1

Query:  592 MHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIF-HLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRI------FSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPE 1149
            +H A+ LH Y+     S ++ L   +++ YA  G +  +R  FD +  K+V TW++M++AY   G  R+A+  F    L S + P+  TF  +L AC +   + +G +I          WD F   +       ++ +  R G +  A +L + M + +D   W A++     + N   A  A++ L+E++ E
Sbjct:   83 VHHAKQLHAYLVVLGKSQNIFLAAKLVNRYAYLGDLSFSRRTFDSIPRKDVYTWNSMVSAYVRSGRFREALDCFSQFSLTSDLHPDFYTFPPVLKACEN---LVDGKKIHCRVLKLGFEWDVFVAAS-------LIHMYSRFGFIYVARKLFDDMPI-RDTGSWNAMISGFCQNAN---AADALDVLVEMRME 261          

BLAST of ppa025850m vs. GDR
Match: MDP0000905763 (MDP0000905763)

HSP 1 Score: 488.419 bits (1256), Expect = 7.764e-138
Identity = 238/553 (43.04%), Postives = 361/553 (65.28%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +HA VV  G   ++ ++  L+ MY++   +G A  +F  M  +  V+W+ M+ G+V   D       F++++  G  P   T+   +  C D+ DL+ G  VH ++ +  L +D     +L+ MY+KCK VD A ++F+K+Q +  V+W  MI  +A+     E+L  F  M  + + PD   MV+++ A A+L    +A+ +H  + R  F  +V + TA++DMYAKCG + SAR++FD M  ++V TW+AMI  YG +G G+ A+ +F+ M    + PN ITF+ ++ ACSHSGL+EEGL+ F+ M +++ +   + HY  MVDLLGRAGRL EA + +++M +E    ++GA+LGACRIH NI+L EKA + + +L P + G++VLL+NIY  A  W  VA +R +M ++ L+K PG + +++ N+ + F +G   HPQS++IY+ L++LG +++ AGYVPDTN + HDV+ +VK  +L SHSEKLAIAFGL+ T  G  I I KNLRVCGDCH  TK++S VT+R IIVRD++RFHHF+ GTCSCGDYW
Sbjct:  281 IHAYVVRAGFESHVNLSTALVDMYSKCGSVGTARLIFXRMKHKTVVSWNSMIDGYVQNEDPKEAMEVFQKMLDQGFEPTNVTIMEALHACADLGDLERGMFVHKLLDQMKLGSDVSVMNSLISMYSKCKRVDIAAKIFNKLQGKTPVSWNAMILGYAQNGRVSEALSHFXEMHSQNIKPDSFTMVSVIPAIAELSVTRQAKWIHGLVIRKCFDKNVFVMTALVDMYAKCGAVHSARKLFDMMDERHVTTWNAMIDGYGTNGLGKSAVDLFNEMKKGTIKPNDITFLCVISACSHSGLVEEGLQYFASMKEDYGLEPAMDHYGAMVDLLGRAGRLYEAWDFIQNMPMEPGITVFGAMLGACRIHKNIELGEKAADKIFKLNPADGGYHVLLANIYQTASLWDKVANVRTMMEKKGLQKTPGCSLVDLKNEVHTFYSGSTSHPQSKRIYSFLDTLGDEIKAAGYVPDTNSI-HDVEADVKEQLLNSHSEKLAIAFGLLNTTPGATIHIRKNLRVCGDCHNATKYISLVTRREIIVRDMHRFHHFKNGTCSCGDYW 832          

HSP 2 Score: 204.142 bits (518), Expect = 2.925e-52
Identity = 107/379 (28.23%), Postives = 206/379 (54.35%), Query Frame = 1

Query:   16 VVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPEN 1152
            ++ NG+        KL+ ++       +A  +F  + ++  V +  ++ G+       + F+ F  +   GV P +Y   ++++VC D  DL+ G+ +H  ++  G  ++ FA  A+V++YAKC+ VD+A ++FD+M  RDLV+W  ++  +A+   A  ++ L   M EEG  PD + +VT++ A A  G++   + +H Y+ R  F   V L TA++DMY+KCG + +AR IF RM+ K V++W++MI  Y  +   ++A+ +F  ML+ G  P  +T +  L+AC+  G +E G+ +  L+ D+  + +D+     ++ +  +  R+D A ++   +   K    W A++     +G +    +A++   E+  +N
Sbjct:   83 IIKNGLYNEHLFQTKLVSLFCNYGCPNEAARVFDTVEDKLEVFYHTLLKGYAKNSSLGDAFSFFCRMKYDGVKPVVYNFTYLLKVCGDNADLRRGKEIHGDLISSGFASNLFAMTAVVNLYAKCRQVDEAYKMFDRMPERDLVSWNTIVAGYAQNGLAKIAMELVIRMQEEGQKPDPITLVTLLPAVADFGSLIIGKSIHAYVVRAGFESHVNLSTALVDMYSKCGSVGTARLIFXRMKHKTVVSWNSMIDGYVQNEDPKEAMEVFQKMLDQGFEPTNVTIMEALHACADLGDLERGMFVHKLL-DQMKLGSDVSVMNSLISMYSKCKRVDIAAKIFNKLQ-GKTPVSWNAMILGYAQNGRV---SEALSHFXEMHSQN 456          

HSP 3 Score: 181.8 bits (460), Expect = 1.554e-45
Identity = 105/381 (27.56%), Postives = 197/381 (51.71%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQ 1143
            E+H  ++ +G   NL     ++ +YA+ R + +AY +F  M ERD V+W+ +V G+   G           +   G  PD  TL  ++    D   L +G+ +H  V++ G  +      ALVDMY+KC  V  AR +F +M+++ +V+W  MI  + +  +  E++ +F  M+++G  P  V ++  ++ACA LG + R   +H  + + +   DV +  ++I MY+KC  +D A +IF+++Q K  ++W+AMI  Y  +G   +A++ F  M +  + P+  T VS++ A +   +  +   I  L+  +     ++   T +VD+  + G +  A +L + M  E+    W A++     +G   L + AV+   E++
Sbjct:  179 EIHGDLISSGFASNLFAMTAVVNLYAKCRQVDEAYKMFDRMPERDLVSWNTIVAGYAQNGLAKIAMELVIRMQEEGQKPDPITLVTLLPAVADFGSLIIGKSIHAYVVRAGFESHVNLSTALVDMYSKCGSVGTARLIFXRMKHKTVVSWNSMIDGYVQNEDPKEAMEVFQKMLDQGFEPTNVTIMEALHACADLGDLERGMFVHKLLDQMKLGSDVSVMNSLISMYSKCKRVDIAAKIFNKLQGKTPVSWNAMILGYAQNGRVSEALSHFXEMHSQNIKPDSFTMVSVIPAIAELSVTRQAKWIHGLVIRK-CFDKNVFVMTALVDMYAKCGAVHSARKLFDMMD-ERHVTTWNAMIDG---YGTNGLGKSAVDLFNEMK 554          

HSP 4 Score: 97.0561 bits (240), Expect = 5.035e-20
Identity = 61/271 (22.51%), Postives = 136/271 (50.18%), Query Frame = 1

Query:  220 RSGVTPDIYTLP--FVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHY----TCMVDLLGRAGRLDEA 1014
            R+ +   +Y  P   ++ +C   ++L     +  +++K+GL  ++     LV ++      ++A ++FD ++++  V +  ++  +A+  +  ++   F  M  +GV P       ++  C     + R + +H  +  + F+ ++   TA++++YAKC  +D A ++FDRM  +++++W+ ++A Y  +G  + A+ +   M   G  P+ IT V+LL A +  G +  G  I +     + VRA  + +    T +VD+  + G +  A
Sbjct:   51 RTHIPSHVYKHPAAILLELCTSXQELNQ---IIPLIIKNGLYNEHLFQTKLVSLFCNYGCPNEAARVFDTVEDKLEVFYHTLLKGYAKNSSLGDAFSFFCRMKYDGVKPVVYNFTYLLKVCGDNADLRRGKEIHGDLISSGFASNLFAMTAVVNLYAKCRQVDEAYKMFDRMPERDLVSWNTIVAGYAQNGLAKIAMELVIRMQEEGQKPDPITLVTLLPAVADFGSLIIGKSIHA-----YVVRAGFESHVNLSTALVDMYSKCGSVGTA 313          

BLAST of ppa025850m vs. GDR
Match: MDP0000276836 (MDP0000276836)

HSP 1 Score: 485.337 bits (1248), Expect = 6.572e-137
Identity = 228/553 (41.23%), Postives = 350/553 (63.29%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +HA  +     +++   N +L MY++   L  A  +F  MGER  V+W+ M+ G +  G        F E+ R GV PD YT+  ++  C     LK GR +H+ + +HG+ +  + C  L+DMYAKC  ++DA  +F +M  +D+V+W  MIG +++    +E+L LF  M+++   PD + + +++ ACA L A++R + +H +I RN +  +  +  A++DMY KCG +  AR +FD + VK++I+W+ + A YG HG GR+AIA F+ M  +G+ P+ ++F+S+LYACSHSGL +E  R F  M +++ +   ++HY CMVDLL R G L +A + +++M +E D  +WG+LL  CRIH ++ LAEK    + EL+PEN G+YVLL+NIYA A +W++V  +R+ +++R LKK PG +WIE+  K   F  GD  HPQ+ KI ++L+ L  K++  G  P   + L + DE  K   L  HSEKLAIAFG++    G  IR+TKNLRVCGDCH   KF+S  ++R I++RD NRFHH ++G CSC  +W
Sbjct:  768 LHAYAIKASFDEDVMFCNNVLDMYSKCGDLSSAIQVFKKMGERSVVSWTSMIAGHIREGLSDEAIGLFSEMERDGVNPDAYTITSILHACASSGSLKKGRDIHNYIKEHGMDSSLYVCNTLMDMYAKCGSMEDAHSVFSRMPAKDIVSWNTMIGGYSKNCLPNEALKLFSEMMQKSK-PDSMTIASVLPACASLAALNRGQEIHGHILRNGYFSERHVANALVDMYVKCGVLVLARLLFDXIPVKDLISWTVIXAGYGMHGFGREAIAAFNEMRKAGIEPDSVSFISILYACSHSGLXDEAWRFFDTMRNDYGIVPKLEHYACMVDLLSRTGNLTKAYKFIKTMPIEPDATIWGSLLCGCRIHHDVKLAEKVAEHVFELEPENTGYYVLLANIYAEAEKWEEVKMLRERITRRGLKKNPGCSWIEIKGKVKIFVAGDSSHPQAAKIESLLKRLRLKMKEEGQSPKMQYALINADEAEKEVALCGHSEKLAIAFGILNLQPGKTIRVTKNLRVCGDCHEMAKFISRTSRREIVLRDSNRFHHMKDGNCSCRGFW 1319          

HSP 2 Score: 176.792 bits (447), Expect = 5.000e-44
Identity = 98/360 (27.22%), Postives = 190/360 (52.78%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTC--MVDLLGRAGRLDEALELVESMAVEKDERLWGALLG 1077
            VH+ +  NG+  +  +  KL++MY +   L +A  +F  +       W++M+  +  V +F  G   F ++   G+  + YT   V++    +  ++ G  +H  + K G  +DN    +L+  Y K + ++ AR++FD++ +RD+++W  MI A+     A++ + +F  M+  G+  D   ++ ++ AC   G +   R LH Y  +  F  DV+    ++DMY+KCG + SA ++F +M  ++V++W++MIA +   G   +AI +F  M   GV+P+  T  S+L+AC+ SG +++G  I + + +      D   Y C  ++D+  + G +++A  +   M   KD   W  ++G
Sbjct:  566 VHSVICDNGVEADGQLGAKLVFMYVKCGDLREARRVFDKLSNGKVFVWNLMINEYAKVRNFREGVYLFGKMQELGIQANSYTFSCVLKCFSALGCVREGEWIHGYLYKLGFGSDNTVGNSLMAFYFKNRRIEXARKVFDELCDRDVISWNSMISAYVSNGLAEKGVEIFRQMVSLGIDVDLATIINVLMACVAGGNLSLGRALHAYAIKASFDEDVMFCNNVLDMYSKCGDLSSAIQVFKKMGERSVVSWTSMIAGHIREGLSDEAIGLFSEMERDGVNPDAYTITSILHACASSGSLKKGRDIHNYIKEH---GMDSSLYVCNTLMDMYAKCGSMEDAHSVFSRMPA-KDIVSWNTMIG 921          

HSP 3 Score: 176.792 bits (447), Expect = 5.000e-44
Identity = 109/393 (27.74%), Postives = 197/393 (50.13%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFS-LMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLE--LQPENAGHYVLL 1173
            +H  +   G   + TV N L+  Y + R +  A  +F  + +RD ++W+ M+  +V+ G    G   FR+++  G+  D+ T+  V+  C    +L +GR +H   +K     D   C  ++DMY+KC  +  A Q+F KM  R +V+WT MI        +DE++ LF  M  +GV PD   + +I++ACA  G++ + R +H YI  +     + +   ++DMYAKCG ++ A  +F RM  K++++W+ MI  Y  +    +A+ +F  M+     P+ +T  S+L AC+    +  G  I   ++ + +     + +   +VD+  + G L  A  L + + V KD   W  +     +HG    A  A N + +  ++P++     +L
Sbjct:  667 IHGYLYKLGFGSDNTVGNSLMAFYFKNRRIEXARKVFDELCDRDVISWNSMISAYVSNGLAEKGVEIFRQMVSLGIDVDLATIINVLMACVAGGNLSLGRALHAYAIKASFDEDVMFCNNVLDMYSKCGDLSSAIQVFKKMGERSVVSWTSMIAGHIREGLSDEAIGLFSEMERDGVNPDAYTITSILHACASSGSLKKGRDIHNYIKEHGMDSSLYVCNTLMDMYAKCGSMEDAHSVFSRMPAKDIVSWNTMIGGYSKNCLPNEALKLFSEMMQKS-KPDSMTIASVLPACASLAALNRGQEIHGHILRNGYFSERHVAN--ALVDMYVKCGVLVLARLLFDXIPV-KDLISWTVIXAGYGMHGFGREAIAAFNEMRKAGIEPDSVSFISIL 1055          

HSP 4 Score: 146.747 bits (369), Expect = 5.541e-35
Identity = 87/318 (27.36%), Postives = 167/318 (52.52%), Query Frame = 1

Query:  259 VIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRA----DIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPE--NAGHYVLLSNIYARA 1194
            V+ +C  M  L+ G+ VH ++  +G+ AD    A LV MY KC  + +AR++FDK+ N  +  W +MI  +A+ RN  E + LF  M E G+  +      ++   + LG +     +H Y+ +  F  D  +G +++  Y K   I+ AR++FD +  ++VI+W++MI+AY  +G   K + IF  M++ G+  ++ T +++L AC   G +  G  + +     +A++A    D+     ++D+  + G L  A+++ + M  E+    W +++     H    L+++A+    E++ +  N   Y + S ++A A
Sbjct:  550 VVELCAGMMSLQDGKXVHSVICDNGVEADGQLGAKLVFMYVKCGDLREARRVFDKLSNGKVFVWNLMINEYAKVRNFREGVYLFGKMQELGIQANSYTFSCVLKCFSALGCVREGEWIHGYLYKLGFGSDNTVGNSLMAFYFKNRRIEXARKVFDELCDRDVISWNSMISAYVSNGLAEKGVEIFRQMVSLGIDVDLATIINVLMACVAGGNLSLGRALHA-----YAIKASFDEDVMFCNNVLDMYSKCGDLSSAIQVFKKMG-ERSVVSWTSMIAG---HIREGLSDEAIGLFSEMERDGVNPDAYTITSILHACA 858          

HSP 5 Score: 123.25 bits (308), Expect = 6.561e-28
Identity = 73/264 (27.65%), Postives = 134/264 (50.76%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILG--TAMIDMYAKCGCIDSAREIFDRMQVK-NVITWSAMIAAYGYH 783
            ++H  +  +GM  +L V N L+ MYA+   + DA+++F  M  +D V+W+ M+GG+            F E+++    PD  T+  V+  C  +  L  G+ +H  +L++G  ++     ALVDMY KC V+  AR LFD +  +DL++WTV+   +       E++  F+ M + G+ PD V+ ++I+ AC+  G    A    +   RN + +   L     M+D+ ++ G +  A +    M ++ +   W +++     H
Sbjct:  868 DIHNYIKEHGMDSSLYVCNTLMDMYAKCGSMEDAHSVFSRMPAKDIVSWNTMIGGYSKNCLPNEALKLFSEMMQKS-KPDSMTIASVLPACASLAALNRGQEIHGHILRNGYFSERHVANALVDMYVKCGVLVLARLLFDXIPVKDLISWTVIXAGYGMHGFGREAIAAFNEMRKAGIEPDSVSFISILYACSHSGLXDEA-WRFFDTMRNDYGIVPKLEHYACMVDLLSRTGNLTKAYKFIKTMPIEPDATIWGSLLCGCRIH 1129          

HSP 6 Score: 76.2554 bits (186), Expect = 9.197e-14
Identity = 51/194 (26.29%), Postives = 91/194 (46.91%), Query Frame = 1

Query:  541 DKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAV 1122
            D  A  ++V  CA + ++   + +H  IC N    D  LG  ++ MY KCG +  AR +FD++    V  W+ MI  Y    + R+ + +F  M   G+  N  TF  +L   S  G + EG  I   ++ +    +D      ++    +  R++ A ++ + +  ++D   W +++ A   +G   LAEK V
Sbjct:  543 DLEAYCSVVELCAGMMSLQDGKXVHSVICDNGVEADGQLGAKLVFMYVKCGDLREARRVFDKLSNGKVFVWNLMINEYAKVRNFREGVYLFGKMQELGIQANSYTFSCVLKCFSALGCVREGEWIHGYLY-KLGFGSDNTVGNSLMAFYFKNRRIEXARKVFDELC-DRDVISWNSMISAYVSNG---LAEKGV 731          

BLAST of ppa025850m vs. GDR
Match: MDP0000296577 (MDP0000296577)

HSP 1 Score: 482.641 bits (1241), Expect = 4.260e-136
Identity = 246/590 (41.69%), Postives = 367/590 (62.20%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRD-------------------------------LVTWTVMIGAFAECRNADESLVLFDWMIEEG--VVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQV--KNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYA-MLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +VHAQ+ + G   +  +  K++ MYA    L  A A+F  +    ++ ++ ++  +   G        +  +   G+  D +T PFV++ C ++  + +G+ VH + LK GL +D +   +L++MY KC  + DAR+LFDKM  RD                               +V+WT MI  + +   A+++L LFD M+++   V P+ V +++++ ACA   A+ R R +H +  R     +V + TA++ MYAKCG +  AR+ F+R++    N++ W+ MI AY  HG G +A++ F  M+ +GV P+ ITF  LL  CSHSGL++ GL+ F  M   ++V   ++HY C+VDLLGRAGRL EA +L+  M ++    +WGA+L ACR H N+++AE A  SL  L+PEN+G+YV+LSNIYA AG WK+V  +R L+  + +KK PG +W EV+ K + F  GD  HPQ+++IY  +L+ L KK++ AGYVPDT+FVLHDV EE K   L +HSEKLAIAFGL+ T+ G  +R+TKNLR+CGDCHT TK +S + +R IIVRD+NRFHHF++G CSCGDYW
Sbjct:   37 QVHAQMALRGFEPDAYLGAKMVAMYASSDDLDSAVAIFHRVNNPSTLLYNSIIRAYTLHGFSEETMEIYGRMHCLGLKXDNFTYPFVLKCCAELSRIWIGKCVHGLSLKVGLESDMYVGTSLINMYVKCCDMSDARRLFDKMTVRDVSSWNALIAGYMKDGEICLAEDLFGKMPGRNIVSWTAMISGYTQNGLAEQALFLFDEMLKKDSKVKPNWVTIMSVLPACAHSAALERGRKIHNFASRIGLESNVSIQTALLAMYAKCGSLLDARQCFERVRXTQNNLVAWNTMITAYASHGRGSEAVSTFEDMIVAGVQPDNITFTGLLSGCSHSGLVDVGLKYFDYMKRVYSVEPGVEHYACVVDLLGRAGRLAEAKDLIXKMPMQAGPSIWGAMLSACRKHHNLEIAEIAARSLFILEPENSGNYVMLSNIYAEAGMWKEVDNLRVLLKAQGVKKNPGCSWTEVNGKAHLFLGGDTSHPQAKEIYEFLLDELPKKIKAAGYVPDTSFVLHDVSEEEKEHSLTTHSEKLAIAFGLLNTSPGVVLRVTKNLRICGDCHTATKLISRIYEREIIVRDLNRFHHFKDGNCSCGDYW 626          

HSP 2 Score: 114.005 bits (284), Expect = 3.980e-25
Identity = 81/332 (24.40%), Postives = 154/332 (46.39%), Query Frame = 1

Query:  289 LKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQV-------------------------------KNVITWSAMIAAYGYHGHGRKAIAIFHLML--NSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERL-WGALLGACRIHGNIDLAEKAVNSLL--ELQPENAGHYVLLS 1176
            LK+G+ VH  +   G   D +  A +V MYA    +D A  +F ++ N   + +  +I A+     ++E++ ++  M   G+  D      ++  CA+L  +   + +H    +     D+ +GT++I+MY KC  +  AR +FD+M V                               +N+++W+AMI+ Y  +G   +A+ +F  ML  +S V PN +T +S+L AC+HS  +E G +I +       + +++   T ++ +  + G L +A +  E +   ++  + W  ++ A   HG    A      ++   +QP+N     LLS
Sbjct:   32 LKLGQQVHAQMALRGFEPDAYLGAKMVAMYASSDDLDSAVAIFHRVNNPSTLLYNSIIRAYTLHGFSEETMEIYGRMHCLGLKXDNFTYPFVLKCCAELSRIWIGKCVHGLSLKVGLESDMYVGTSLINMYVKCCDMSDARRLFDKMTVRDVSSWNALIAGYMKDGEICLAEDLFGKMPGRNIVSWTAMISGYTQNGLAEQALFLFDEMLKKDSKVKPNWVTIMSVLPACAHSAALERGRKIHNFA-SRIGLESNVSIQTALLAMYAKCGSLLDARQCFERVRXTQNNLVAWNTMITAYASHGRGSEAVSTFEDMIVAGVQPDNITFTGLLS 362          

BLAST of ppa025850m vs. GDR
Match: MDP0000222134 (MDP0000222134)

HSP 1 Score: 482.641 bits (1241), Expect = 4.260e-136
Identity = 246/590 (41.69%), Postives = 367/590 (62.20%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRD-------------------------------LVTWTVMIGAFAECRNADESLVLFDWMIEEG--VVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQV--KNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYA-MLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +VHAQ+ + G   +  +  K++ MYA    L  A A+F  +    ++ ++ ++  +   G        +  +   G+  D +T PFV++ C ++  + +G+ VH + LK GL +D +   +L++MY KC  + DAR+LFDKM  RD                               +V+WT MI  + +   A+++L LFD M+++   V P+ V +++++ ACA   A+ R R +H +  R     +V + TA++ MYAKCG +  AR+ F+R++    N++ W+ MI AY  HG G +A++ F  M+ +GV P+ ITF  LL  CSHSGL++ GL+ F  M   ++V   ++HY C+VDLLGRAGRL EA +L+  M ++    +WGA+L ACR H N+++AE A  SL  L+PEN+G+YV+LSNIYA AG WK+V  +R L+  + +KK PG +W EV+ K + F  GD  HPQ+++IY  +L+ L KK++ AGYVPDT+FVLHDV EE K   L +HSEKLAIAFGL+ T+ G  +R+TKNLR+CGDCHT TK +S + +R IIVRD+NRFHHF++G CSCGDYW
Sbjct:   37 QVHAQMALRGFEPDAYLGAKMVAMYASSDDLDSAVAIFHRVNNPSTLLYNSIIRAYTLHGFSEETMEIYGRMHCLGLKXDNFTYPFVLKCCAELSRIWIGKCVHGLSLKVGLESDMYVGTSLINMYVKCCDMSDARRLFDKMTVRDVSSWNALIAGYMKDGEICLAEDLFGKMPGRNIVSWTAMISGYTQNGLAEQALFLFDEMLKKDSKVKPNWVTIMSVLPACAHSAALERGRKIHNFASRIGLESNVSIQTALLAMYAKCGSLLDARQCFERVRXTQNNLVAWNTMITAYASHGRGSEAVSTFEDMIVAGVQPDNITFTGLLSGCSHSGLVDVGLKYFDYMKRVYSVEPGVEHYACVVDLLGRAGRLAEAKDLIXKMPMQAGPSIWGAMLSACRKHHNLEIAEIAARSLFILEPENSGNYVMLSNIYAEAGMWKEVDNLRVLLKAQGVKKNPGCSWTEVNGKAHLFLGGDTSHPQAKEIYEFLLDELPKKIKAAGYVPDTSFVLHDVSEEEKEHSLTTHSEKLAIAFGLLNTSPGVVLRVTKNLRICGDCHTATKLISRIYEREIIVRDLNRFHHFKDGNCSCGDYW 626          

HSP 2 Score: 114.005 bits (284), Expect = 3.980e-25
Identity = 81/332 (24.40%), Postives = 154/332 (46.39%), Query Frame = 1

Query:  289 LKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQV-------------------------------KNVITWSAMIAAYGYHGHGRKAIAIFHLML--NSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERL-WGALLGACRIHGNIDLAEKAVNSLL--ELQPENAGHYVLLS 1176
            LK+G+ VH  +   G   D +  A +V MYA    +D A  +F ++ N   + +  +I A+     ++E++ ++  M   G+  D      ++  CA+L  +   + +H    +     D+ +GT++I+MY KC  +  AR +FD+M V                               +N+++W+AMI+ Y  +G   +A+ +F  ML  +S V PN +T +S+L AC+HS  +E G +I +       + +++   T ++ +  + G L +A +  E +   ++  + W  ++ A   HG    A      ++   +QP+N     LLS
Sbjct:   32 LKLGQQVHAQMALRGFEPDAYLGAKMVAMYASSDDLDSAVAIFHRVNNPSTLLYNSIIRAYTLHGFSEETMEIYGRMHCLGLKXDNFTYPFVLKCCAELSRIWIGKCVHGLSLKVGLESDMYVGTSLINMYVKCCDMSDARRLFDKMTVRDVSSWNALIAGYMKDGEICLAEDLFGKMPGRNIVSWTAMISGYTQNGLAEQALFLFDEMLKKDSKVKPNWVTIMSVLPACAHSAALERGRKIHNFA-SRIGLESNVSIQTALLAMYAKCGSLLDARQCFERVRXTQNNLVAWNTMITAYASHGRGSEAVSTFEDMIVAGVQPDNITFTGLLS 362          

BLAST of ppa025850m vs. NCBI nr
Match: gi|356498743|ref|XP_003518209.1| (PREDICTED: pentatricopeptide repeat-containing protein At3g46790, chloroplastic-like [Glycine max])

HSP 1 Score: 859.366 bits (2219), Expect = 0.000e+0
Identity = 411/554 (74.19%), Postives = 479/554 (86.46%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +VHA VV NG LQ+L +ANKLLY YAQ + + DAY+LF G+  RDS TWSVMVGGF   GD    +ATFREL+R GVTPD YTLPFVIR CRD  DL++GR++HD+VLKHGLL+D+F CA+LVDMYAKC VV+DA++LF++M ++DLVTWTVMIGA+A+C NA ESLVLFD M EEGVVPDKVAMVT+VNACAKLGAMHRAR  + YI RN FSLDVILGTAMIDMYAKCG ++SARE+FDRM+ KNVI+WSAMIAAYGYHG G+ AI +FH+ML+  + PN +TFVSLLYACSH+GLIEEGLR F+ MW+E AVR D+KHYTCMVDLLGRAGRLDEAL L+E+M VEKDERLW ALLGACRIH  ++LAEKA NSLLELQP+N GHYVLLSNIYA+AG+W+ VAK RD+M+QR+LKK+PG TWIEVDNKTYQFS GDR HPQS++IY ML SL KKLE+AGYVPDT+FVL DV+EEVK  MLY+HSEKLAIAFGLIA  +G PIRI+KNLRVCGDCHTF+K VS++ +R IIVRD NRFHHF +GTCSCGDYW
Sbjct:   21 QVHAHVVANGTLQDLVIANKLLYTYAQHKAIDDAYSLFDGLTMRDSKTWSVMVGGFAKAGDHAGCYATFRELLRCGVTPDNYTLPFVIRTCRDRTDLQIGRVIHDVVLKHGLLSDHFVCASLVDMYAKCIVVEDAQRLFERMLSKDLVTWTVMIGAYADC-NAYESLVLFDRMREEGVVPDKVAMVTVVNACAKLGAMHRARFANDYIVRNGFSLDVILGTAMIDMYAKCGSVESAREVFDRMKEKNVISWSAMIAAYGYHGRGKDAIDLFHMMLSCAILPNRVTFVSLLYACSHAGLIEEGLRFFNSMWEEHAVRPDVKHYTCMVDLLGRAGRLDEALRLIEAMTVEKDERLWSALLGACRIHSKMELAEKAANSLLELQPQNPGHYVLLSNIYAKAGKWEKVAKFRDMMTQRKLKKIPGWTWIEVDNKTYQFSVGDRSHPQSKEIYEMLMSLIKKLEMAGYVPDTDFVLQDVEEEVKQEMLYTHSEKLAIAFGLIAIPEGEPIRISKNLRVCGDCHTFSKMVSSIMRRSIIVRDANRFHHFNDGTCSCGDYW 573          

HSP 2 Score: 134.035 bits (336), Expect = 7.611e-29
Identity = 78/269 (29.00%), Postives = 139/269 (51.67%), Query Frame = 1

Query:  301 RLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVR----ADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            R VH  V+ +G L D      L+  YA+ K +DDA  LFD +  RD  TW+VM+G FA+  +       F  ++  GV PD   +  ++  C     +   R++H  + ++    D  +  +++DMYAKC  ++ A+ +F+RM  K+++TW+ MI AY    +  +++ +F  M   GV P+ +  V+++ AC+  G +       +   +++ VR     D+   T M+D+  + G ++ A E+ + M  EK+   W A++ A   HG
Sbjct:   20 RQVHAHVVANGTLQDLVIANKLLYTYAQHKAIDDAYSLFDGLTMRDSKTWSVMVGGFAKAGDHAGCYATFRELLRCGVTPDNYTLPFVIRTCRDRTDLQIGRVIHDVVLKHGLLSDHFVCASLVDMYAKCIVVEDAQRLFERMLSKDLVTWTVMIGAYA-DCNAYESLVLFDRMREEGVVPDKVAMVTVVNACAKLGAMHR-----ARFANDYIVRNGFSLDVILGTAMIDMYAKCGSVESAREVFDRMK-EKNVISWSAMIAAYGYHG 281          

BLAST of ppa025850m vs. NCBI nr
Match: gi|147841045|emb|CAN68552.1| (hypothetical protein VITISV_014227 [Vitis vinifera])

HSP 1 Score: 853.203 bits (2203), Expect = 0.000e+0
Identity = 410/554 (74.01%), Postives = 469/554 (84.66%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +VH Q  V+GMLQNL VANKL+  Y+  R L DAY LF GM  RDSV+WSVMVGGF  VGD+ N F TFRELIR G  PD YTLPFVIR CRD+K+L+MGRL+H IV K GL  D+F CAALVDMY KC+ ++DAR LFDKM  RDLVTWTVMIG +AEC NA+ESLVLFD M EEGVVPDKVAMVT+V ACAKLGAMH+AR +  YI R +F LDVILGTAMIDM+AKCGC++SAREIFDRM+ KNVI+WSAMIAAYGYHG GRKA+ +F +ML SG+ PN IT VSLLYACSH+GL+EEGLR FS MW++++VR D+KHYTC+VDLLGRAGRLDEAL+L+ SM  EKDE LWGA LGACR H ++ LAEKA  SLLELQP+N GHY+LLSNIYA AGRW+DVAK+RDLMSQRRLKK+PG TWIEVDNK++QFS GD  HP+S++IY ML+SLG KLEL GYVPDTNFVLHDVDEE+K+ +LY+HSEKLAIAFGLIAT + TPIRI KNLRVCGDCHTF K VSA+T RVIIVRD NRFHHF+EG CSCGDYW
Sbjct:  756 QVHXQASVHGMLQNLIVANKLVXFYSYYRALDDAYGLFDGMCVRDSVSWSVMVGGFAKVGDYMNCFGTFRELIRCGARPDNYTLPFVIRACRDLKNLQMGRLIHHIVYKFGLDLDHFVCAALVDMYGKCREIEDARFLFDKMXERDLVTWTVMIGGYAECGNANESLVLFDKMREEGVVPDKVAMVTVVFACAKLGAMHKARTIDDYIQRKKFQLDVILGTAMIDMHAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFPMMLRSGILPNKITLVSLLYACSHAGLVEEGLRFFSXMWEDYSVRXDVKHYTCVVDLLGRAGRLDEALKLIXSMTXEKDEGLWGAFLGACRTHKDVXLAEKAATSLLELQPQNPGHYILLSNIYANAGRWEDVAKIRDLMSQRRLKKIPGWTWIEVDNKSHQFSVGDTTHPRSKEIYEMLKSLGNKLELVGYVPDTNFVLHDVDEELKIGILYTHSEKLAIAFGLIATPEHTPIRIIKNLRVCGDCHTFCKLVSAITGRVIIVRDANRFHHFKEGACSCGDYW 1309          

HSP 2 Score: 768.844 bits (1984), Expect = 0.000e+0
Identity = 372/512 (72.66%), Postives = 434/512 (84.77%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLR 1536
            +VHAQ  V+GML+N+ VANKL+Y Y+  R L DAY LF GM  RDSV+WSVMVGGF  VGD+ N F TFRELIR G  PD YTLPFVIR CRD+K+L+MGRL+H IV K GL  D+F CAALVDMY KC+ ++DAR LFDKMQ RDLVTWTVMIG +AEC  A+ESLVLF+ M EEGVVPDKVAMVT+V ACAKLGAMH+AR++  YI R +F LDVILGTAMIDMYAKCGC++SAREIFDRM+ KNVI+WSAMIAAYGYHG GRKA+ +F +ML+SG+ P+ IT  SLLYACSH+GL+EEGLR FS MW++++VR D+KHYTC+VDLLGRAGRLDEAL+L++SM +EKDE LWGA LGACR H ++ LAEKA  SLLELQ +N GHYVLLSNIYA AGRW+DVAK+RDLMSQRRLKK PG TWIEVDNK++QFS GD  HP+S++IY ML+SL  KLEL GYVPDTNFVLHDVDEE+K+ +LY+HSEKLAIAFGLIAT + TPIRI KNLR
Sbjct:   80 QVHAQASVHGMLENIVVANKLIYFYSYYRALDDAYGLFDGMCVRDSVSWSVMVGGFAKVGDYINCFGTFRELIRCGARPDNYTLPFVIRACRDLKNLQMGRLIHHIVYKFGLDLDHFVCAALVDMYVKCREIEDARFLFDKMQERDLVTWTVMIGGYAECGKANESLVLFEKMREEGVVPDKVAMVTVVFACAKLGAMHKARIIDDYIQRKKFQLDVILGTAMIDMYAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFPMMLSSGMLPDKITLASLLYACSHAGLVEEGLRFFSSMWEDYSVRTDVKHYTCVVDLLGRAGRLDEALKLIKSMTIEKDEGLWGAFLGACRTHKDVVLAEKAATSLLELQSQNPGHYVLLSNIYANAGRWEDVAKIRDLMSQRRLKKTPGWTWIEVDNKSHQFSVGDTTHPRSKEIYEMLKSLSNKLELVGYVPDTNFVLHDVDEELKIGILYTHSEKLAIAFGLIATPEHTPIRIIKNLR 591          

HSP 3 Score: 150.984 bits (380), Expect = 6.016e-34
Identity = 85/280 (30.36%), Postives = 144/280 (51.43%), Query Frame = 1

Query:  256 FVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            F I    + ++L   R VH     HG+L +      L+  Y+  + +DDA  LFD M  RD V+W+VM+G FA+  +       F  +I  G  PD   +  ++ AC  L  +   RL+H+ + +    LD  +  A++DMY KC  I+ AR +FD+MQ ++++TW+ MI  Y   G   +++ +F  M   GV P+ +  V++++AC+  G + +  RI          + D+   T M+D+  + G ++ A E+ + M  EK+   W A++ A   HG
Sbjct:   64 FYISALVNCRNLTQVRQVHAQASVHGMLENIVVANKLIYFYSYYRALDDAYGLFDGMCVRDSVSWSVMVGGFAKVGDYINCFGTFRELIRCGARPDNYTLPFVIRACRDLKNLQMGRLIHHIVYKFGLDLDHFVCAALVDMYVKCREIEDARFLFDKMQERDLVTWTVMIGGYAECGKANESLVLFEKMREEGVVPDKVAMVTVVFACAKLGAMHKA-RIIDDYIQRKKFQLDVILGTAMIDMYAKCGCVESAREIFDRME-EKNVISWSAMIAAYGYHG 341          

HSP 4 Score: 147.132 bits (370), Expect = 8.687e-33
Identity = 84/274 (30.66%), Postives = 142/274 (51.82%), Query Frame = 1

Query:  286 DLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVR----ADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            +L   R VH     HG+L +      LV  Y+  + +DDA  LFD M  RD V+W+VM+G FA+  +       F  +I  G  PD   +  ++ AC  L  +   RL+H+ + +    LD  +  A++DMY KC  I+ AR +FD+M  ++++TW+ MI  Y   G+  +++ +F  M   GV P+ +  V++++AC+  G + +   I     D++  R     D+   T M+D+  + G ++ A E+ + M  EK+   W A++ A   HG
Sbjct:  750 NLTQVRQVHXQASVHGMLQNLIVANKLVXFYSYYRALDDAYGLFDGMCVRDSVSWSVMVGGFAKVGDYMNCFGTFRELIRCGARPDNYTLPFVIRACRDLKNLQMGRLIHHIVYKFGLDLDHFVCAALVDMYGKCREIEDARFLFDKMXERDLVTWTVMIGGYAECGNANESLVLFDKMREEGVVPDKVAMVTVVFACAKLGAMHKARTI-----DDYIQRKKFQLDVILGTAMIDMHAKCGCVESAREIFDRME-EKNVISWSAMIAAYGYHG 1017          

BLAST of ppa025850m vs. NCBI nr
Match: gi|224140095|ref|XP_002323422.1| (predicted protein [Populus trichocarpa] >gi|222868052|gb|EEF05183.1| predicted protein [Populus trichocarpa])

HSP 1 Score: 795.808 bits (2054), Expect = 0.000e+0
Identity = 383/554 (69.13%), Postives = 455/554 (82.13%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            +VHAQV   G++ +L VANKLLYM A+ + L  A+ LF  M ERD V+WSVM+GGFV  GD+   F TFRELIR+G  PD ++LPFVI+ CRD   L MGRL+H  VLK+GL  DNF C+ LVDMYAKC ++D+A+QLFD+M  +DLVT TVMI  +AEC   +ES VLFD M  +G VPDKVAMVTIVNACAKLGAM++ARL+H Y+C  ++SLDV LGTAMIDMYAKCG IDS+REIFDRM+ KNVI+WSAMI AYGYHG GR+A+ +FH+MLNSG+ PN ITF+SLLYACSH+GL+++GL++FSLM   + VR D+KHYTCMVDLLGRAGRLD+AL L+E+M VEKDE +W A LGACRIH  +DLAEKA   LL LQ +N GHY+LLSNIYA AGRWKDVAK+R+LM++RRLKK+PG TWIEVDN  Y+F  GD  H +S +IY ML+SL +KLE AGYVPDTN VLHDVDEEVKL +L++HSEKLAIAFGLIAT DGTPIRITKNLRVCGDCH+F K VSA+TQR IIVRD NRFHHF+EG CSCGDYW
Sbjct:   21 QVHAQVTTTGIIHDLIVANKLLYMCAKHKDLVTAHLLFNKMEERDPVSWSVMIGGFVKNGDYERCFQTFRELIRAGSKPDNFSLPFVIKACRDTMGLIMGRLIHSTVLKNGLHLDNFVCSTLVDMYAKCGMIDNAKQLFDRMPKKDLVTRTVMIAGYAECGKPNESWVLFDQMRRDGFVPDKVAMVTIVNACAKLGAMNKARLVHDYVCARRYSLDVELGTAMIDMYAKCGSIDSSREIFDRMEQKNVISWSAMIGAYGYHGQGREALELFHMMLNSGIIPNRITFISLLYACSHAGLVDDGLQLFSLMSVSYGVRPDVKHYTCMVDLLGRAGRLDQALRLIENMEVEKDEGIWCAFLGACRIHRQVDLAEKAAKLLLSLQTQNPGHYILLSNIYANAGRWKDVAKIRNLMAKRRLKKIPGYTWIEVDNIIYRFGAGDNSHLRSNEIYEMLKSLSQKLESAGYVPDTNSVLHDVDEEVKLGILHAHSEKLAIAFGLIATPDGTPIRITKNLRVCGDCHSFCKLVSAITQRDIIVRDANRFHHFKEGICSCGDYW 574          

BLAST of ppa025850m vs. NCBI nr
Match: gi|225440839|ref|XP_002276333.1| (PREDICTED: pentatricopeptide repeat-containing protein At1g11290-like [Vitis vinifera])

HSP 1 Score: 775.778 bits (2002), Expect = 0.000e+0
Identity = 373/541 (68.95%), Postives = 443/541 (81.89%), Query Frame = 1

Query:   40 NLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            ++ +   ++ MYA+   +  A  +F  M E++ ++WS M+  +   G        FR ++ SG+ PD  TL  ++  C D+K+L+MGRL+H IV K GL  D+F CAALVDMY KC+ ++DAR LFDKM  RDLVTWTVMIG +AEC NA+ESLVLFD M EEGVVPDKVAMVT+V ACAKLGAMH+AR +  YI R +F LDVILGTAMIDM+AKCGC++SAREIFDRM+ KNVI+WSAMIAAYGYHG GRKA+ +F +ML SG+ PN IT VSLLYACSH+GL+EEGLR FSLMW++++VRAD+KHYTC+VDLLGRAGRLDEAL+L+ESM VEKDE LWGA LGACR H ++ LAEKA  SLLELQP+N GHY+LLSNIYA AGRW+DVAK RDLMSQRRLKK+PG TWIEVDNK++QFS GD  HP+S++IY ML+SLG KLEL GYVPDTNFVLHDVDEE+K+ +LYSHSEKLAIAFGLIAT + TPIRI KNLRVCGDCHTF K VSA+T RVIIVRD NRFHHF+EG CSCGDYW
Sbjct:  165 DVILGTAMIDMYAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFRMMLSSGMLPDKITLASLLYACSDLKNLQMGRLIHHIVYKFGLDLDHFVCAALVDMYGKCREIEDARFLFDKMPERDLVTWTVMIGGYAECGNANESLVLFDKMREEGVVPDKVAMVTVVFACAKLGAMHKARTIDDYIQRKKFQLDVILGTAMIDMHAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFPMMLRSGILPNKITLVSLLYACSHAGLVEEGLRFFSLMWEDYSVRADVKHYTCVVDLLGRAGRLDEALKLIESMTVEKDEGLWGAFLGACRTHKDVVLAEKAATSLLELQPQNPGHYILLSNIYANAGRWEDVAKTRDLMSQRRLKKIPGWTWIEVDNKSHQFSVGDTTHPRSKEIYEMLKSLGNKLELVGYVPDTNFVLHDVDEELKIGILYSHSEKLAIAFGLIATPEHTPIRIIKNLRVCGDCHTFCKLVSAITGRVIIVRDANRFHHFKEGACSCGDYW 705          

HSP 2 Score: 384.03 bits (985), Expect = 4.222e-104
Identity = 195/317 (61.51%), Postives = 241/317 (76.03%), Query Frame = 1

Query:  154 MVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTC--MVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGN 1098
            MVGGF  VGD+ N F TFRELIR G  PD YTLPFVIR CRD+K+L+MGRL+H IV K GL  D+F CAALVDMY KC+ ++DAR LFDKMQ RDLVTWTVMIG +AEC  A+ESLVLF+ M EEGVVPDKVAMVT+V ACAKLGAMH+AR++  YI R +F LDVILGTAMIDMYAKCGC++SAREIFDRM+ KNVI+WSAMIAAYGYHG GRKA+ +F +ML+SG+ P+ IT  SLLYACS    ++ G  I  +++ +F +  D+ H+ C  +VD+ G+   +++A  L + M  E+D   W  ++G     GN
Sbjct:    1 MVGGFAKVGDYINCFGTFRELIRCGARPDNYTLPFVIRACRDLKNLQMGRLIHHIVYKFGLDLDHFVCAALVDMYVKCREIEDARFLFDKMQERDLVTWTVMIGGYAECGKANESLVLFEKMREEGVVPDKVAMVTVVFACAKLGAMHKARIIDDYIQRKKFQLDVILGTAMIDMYAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFRMMLSSGMLPDKITLASLLYACSDLKNLQMGRLIHHIVY-KFGL--DLDHFVCAALVDMYGKCREIEDARFLFDKMP-ERDLVTWTVMIGGYAECGN 313          

HSP 3 Score: 214.157 bits (544), Expect = 5.787e-53
Identity = 112/368 (30.43%), Postives = 199/368 (54.08%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVR----ADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            +H  V   G+  +  V   L+ MY + R + DA  LF  M ERD VTW+VM+GG+   G        F ++   GV PD   +  V+  C  +  +   R++ D + +     D     A++DMYAKC  V+ AR++FD+M+ +++++W+ MI A+       ++L LF  M+  G++PDK+ + +++ AC+ L  +   RL+H+ + +    LD  +  A++DMY KC  I+ AR +FD+M  ++++TW+ MI  Y   G+  +++ +F  M   GV P+ +  V++++AC+  G + +   I     D++  R     D+   T M+D+  + G ++ A E+ + M  EK+   W A++ A   HG
Sbjct:   52 IHHIVYKFGLDLDHFVCAALVDMYVKCREIEDARFLFDKMQERDLVTWTVMIGGYAECGKANESLVLFEKMREEGVVPDKVAMVTVVFACAKLGAMHKARIIDDYIQRKKFQLDVILGTAMIDMYAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFRMMLSSGMLPDKITLASLLYACSDLKNLQMGRLIHHIVYKFGLDLDHFVCAALVDMYGKCREIEDARFLFDKMPERDLVTWTVMIGGYAECGNANESLVLFDKMREEGVVPDKVAMVTVVFACAKLGAMHKARTI-----DDYIQRKKFQLDVILGTAMIDMHAKCGCVESAREIFDRME-EKNVISWSAMIAAYGYHG 413          

HSP 4 Score: 138.272 bits (347), Expect = 4.035e-30
Identity = 77/263 (29.28%), Postives = 138/263 (52.47%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSL--DVILGTAMIDMYAKCGCIDSAREIFDRMQV-KNVITWSAMIAAYGYH 783
            +H  V   G+  +  V   L+ MY + R + DA  LF  M ERD VTW+VM+GG+   G+       F ++   GV PD   +  V+  C  +  +   R + D + +     D     A++DM+AKC  V+ AR++FD+M+ +++++W+ MI A+       ++L LF  M+  G++P+K+ +V+++ AC+  G +    L  + +    +S+  DV   T ++D+  + G +D A ++ + M V K+   W A + A   H
Sbjct:  254 IHHIVYKFGLDLDHFVCAALVDMYGKCREIEDARFLFDKMPERDLVTWTVMIGGYAECGNANESLVLFDKMREEGVVPDKVAMVTVVFACAKLGAMHKARTIDDYIQRKKFQLDVILGTAMIDMHAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFPMMLRSGILPNKITLVSLLYACSHAGLVEEG-LRFFSLMWEDYSVRADVKHYTCVVDLLGRAGRLDEALKLIESMTVEKDEGLWGAFLGACRTH 515          

BLAST of ppa025850m vs. NCBI nr
Match: gi|297740136|emb|CBI30318.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 709.523 bits (1830), Expect = 0.000e+0
Identity = 353/569 (62.04%), Postives = 426/569 (74.87%), Query Frame = 1

Query:   40 NLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAE----------------------------CRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            ++ +   ++ MYA+   +  A  +F  M E++ ++WS M+  +   G        FR ++ SG+ PD  TL  ++  C + ++L   R VH     HG+L +      LV  Y+  + +DDA  LFD M  RD V+W+VM+G FA+                            C NA+ESLVLFD M EEGVVPDKVAMVT+V ACAKLGAMH+AR +  YI R +F LDVILGTAMIDM+AKCGC++SAREIFDRM+ KNVI+WSAMIAAYGYHG GRKA+ +F +ML SG+ PN IT VSLLYACSH+GL+EEGLR FSLMW++++VRAD+KHYTC+VDLLGRAGRLDEAL+L+ESM VEKDE LWGA LGACR H ++ LAEKA  SLLELQP+N GHY+LLSNIYA AGRW+DVAK RDLMSQRRLKK+PG TWIEVDNK++QFS GD  HP+S++IY ML+SLG KLEL GYVPDTNFVLHDVDEE+K+ +LYSHSEKLAIAFGLIAT + TPIRI KNLRVCGDCHTF K VSA+T RVIIVRD NRFHHF+EG CSCGDYW
Sbjct:  293 DVILGTAMIDMYAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFRMMLSSGMLPDKITLASLLYACINCRNLTQVRQVHAQASVHGMLQNLIVANKLVHFYSYYRALDDAYGLFDGMCVRDSVSWSVMVGGFAKVGDYMNCFGTFRELIRCGARPDNYTLPFCGNANESLVLFDKMREEGVVPDKVAMVTVVFACAKLGAMHKARTIDDYIQRKKFQLDVILGTAMIDMHAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFPMMLRSGILPNKITLVSLLYACSHAGLVEEGLRFFSLMWEDYSVRADVKHYTCVVDLLGRAGRLDEALKLIESMTVEKDEGLWGAFLGACRTHKDVVLAEKAATSLLELQPQNPGHYILLSNIYANAGRWEDVAKTRDLMSQRRLKKIPGWTWIEVDNKSHQFSVGDTTHPRSKEIYEMLKSLGNKLELVGYVPDTNFVLHDVDEELKIGILYSHSEKLAIAFGLIATPEHTPIRIIKNLRVCGDCHTFCKLVSAITGRVIIVRDANRFHHFKEGACSCGDYW 861          

HSP 2 Score: 391.349 bits (1004), Expect = 2.645e-106
Identity = 206/362 (56.91%), Postives = 255/362 (70.44%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTC---MVDLLGRAGRLDEALELVESMAVEKDERLWGALLG 1077
            +VHAQ  V+GML+N+ VANKL+Y Y+  R L DAY LF GM  RDSV+WSVMVGGF  VGD+ N F TFRELIR G  PD YTLPFVIR CRD+K+L+M                     ALVDMY KC+ ++DAR LFDKMQ RDLVTWTVMIG +AEC  A+ESLVLF+ M EEGVVPDKVAMVT+V ACAKLGAMH+AR++  YI R +F LDVILGTAMIDMYAKCGC++SAREIFDRM+ KNVI+WSAMIAAYGYHG GRKA+ +F +ML+SG+ P+ IT  SLLYAC +   + +  ++ +    + +V   +++      +V        LD+A  L + M V +D   W  ++G
Sbjct:   99 QVHAQASVHGMLENIVVANKLIYFYSYYRALDDAYGLFDGMCVRDSVSWSVMVGGFAKVGDYINCFGTFRELIRCGARPDNYTLPFVIRACRDLKNLQM---------------------ALVDMYVKCREIEDARFLFDKMQERDLVTWTVMIGGYAECGKANESLVLFEKMREEGVVPDKVAMVTVVFACAKLGAMHKARIIDDYIQRKKFQLDVILGTAMIDMYAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFRMMLSSGMLPDKITLASLLYACINCRNLTQVRQVHA----QASVHGMLQNLIVANKLVHFYSYYRALDDAYGLFDGMCV-RDSVSWSVMVG 434          

HSP 3 Score: 191.045 bits (484), Expect = 5.246e-46
Identity = 106/292 (36.30%), Postives = 161/292 (55.14%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPF----------------------------VIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSL--DVILGTAMIDMYAKCGCIDSAREIFDRMQV-KNVITWSAMIAAYGYH 783
            +VHAQ  V+GMLQNL VANKL++ Y+  R L DAY LF GM  RDSV+WSVMVGGF  VGD+ N F TFRELIR G  PD YTLPF                            V+  C  +  +   R + D + +     D     A++DM+AKC  V+ AR++FD+M+ +++++W+ MI A+       ++L LF  M+  G++P+K+ +V+++ AC+  G +    L  + +    +S+  DV   T ++D+  + G +D A ++ + M V K+   W A + A   H
Sbjct:  381 QVHAQASVHGMLQNLIVANKLVHFYSYYRALDDAYGLFDGMCVRDSVSWSVMVGGFAKVGDYMNCFGTFRELIRCGARPDNYTLPFCGNANESLVLFDKMREEGVVPDKVAMVTVVFACAKLGAMHKARTIDDYIQRKKFQLDVILGTAMIDMHAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFPMMLRSGILPNKITLVSLLYACSHAGLVEEG-LRFFSLMWEDYSVRADVKHYTCVVDLLGRAGRLDEALKLIESMTVEKDEGLWGAFLGACRTH 671          

HSP 4 Score: 176.792 bits (447), Expect = 1.024e-41
Identity = 102/386 (26.42%), Postives = 190/386 (49.22%), Query Frame = 1

Query:   34 LQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYG----------------------------YHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVR----ADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            L+NL +A  L+ MY + R + DA  LF  M ERD VTW+VM+GG+   G        F ++   GV PD   +  V+  C  +  +   R++ D + +     D     A++DMYAKC  V+ AR++FD+M+ +++++W+ MI A+       ++L LF  M+  G++PDK+ + +++ AC     + + R +H     +    ++I+   ++  Y+    +D A  +FD M V++ ++WS M+  +                             + G+  +++ +F  M   GV P+ +  V++++AC+  G + +   I     D++  R     D+   T M+D+  + G ++ A E+ + M  EK+   W A++ A   HG
Sbjct:  192 LKNLQMA--LVDMYVKCREIEDARFLFDKMQERDLVTWTVMIGGYAECGKANESLVLFEKMREEGVVPDKVAMVTVVFACAKLGAMHKARIIDDYIQRKKFQLDVILGTAMIDMYAKCGCVESAREIFDRMEEKNVISWSAMIAAYGYHGQGRKALDLFRMMLSSGMLPDKITLASLLYACINCRNLTQVRQVHAQASVHGMLQNLIVANKLVHFYSYYRALDDAYGLFDGMCVRDSVSWSVMVGGFAKVGDYMNCFGTFRELIRCGARPDNYTLPFCGNANESLVLFDKMREEGVVPDKVAMVTVVFACAKLGAMHKARTI-----DDYIQRKKFQLDVILGTAMIDMHAKCGCVESAREIFDRME-EKNVISWSAMIAAYGYHG 569          

BLAST of ppa025850m vs. NCBI nr
Match: gi|255578965|ref|XP_002530335.1| (Bipolar kinesin KRP-130, putative [Ricinus communis] >gi|223530139|gb|EEF32051.1| Bipolar kinesin KRP-130, putative [Ricinus communis])

HSP 1 Score: 614.379 bits (1583), Expect = 1.921e-173
Identity = 294/428 (68.69%), Postives = 357/428 (83.41%), Query Frame = 1

Query:    1 EVHAQVVVNGML-QNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEV 1281
            ++HA +  N +L  NLTVANKLLY+Y   + L DAYALF  M +++ V+WS+M+GGFV VG+F   + TF+ELIR+GV PD YTLPFVI+ CRD   L MGRL+H +VLK+GL  D+F CAALVDMYAKCKV++DA+ LFD M ++DLVTWTVMIG +AEC NA ESLVLFD + EEG VPDK+AMV+IVNACAKLGAM++AR +H Y+ RN+FSLDVILGTAMIDMYAKCG +DSAREIFD M  KNVI+WSAMIAAYGYHG G+KA+ +F +ML+SG+ PN ITFVSLLYACSH+GL+E+GL + SLMW+ F VR DIKH+TCMVDLLGRAG+L EA   +E+M VEKDE LW +LLGACRIH  IDLAEKA  SLLELQP+N G+Y+LLSNIYA AG+WKDVAK+RDLM++R+LKK+PG TWIE+
Sbjct: 1100 QLHALITTNSVLYNNLTVANKLLYVYVHHKCLTDAYALFDEMPQKNPVSWSIMIGGFVKVGEFMQCYKTFKELIRNGVQPDNYTLPFVIKACRDTVALDMGRLIHCVVLKYGLHLDHFVCAALVDMYAKCKVIEDAKLLFDVMPSKDLVTWTVMIGGYAECGNAKESLVLFDHLREEGFVPDKIAMVSIVNACAKLGAMNKARFVHEYVNRNRFSLDVILGTAMIDMYAKCGDVDSAREIFDNMHHKNVISWSAMIAAYGYHGQGKKALELFPMMLSSGILPNNITFVSLLYACSHAGLVEDGLTLLSLMWESFGVRPDIKHHTCMVDLLGRAGQLTEAFRFIENMTVEKDEGLWSSLLGACRIHKRIDLAEKAAKSLLELQPQNPGNYILLSNIYANAGQWKDVAKIRDLMTKRKLKKIPGYTWIEL 1527          

BLAST of ppa025850m vs. NCBI nr
Match: gi|359488803|ref|XP_002272525.2| (PREDICTED: putative pentatricopeptide repeat-containing protein At5g09950 [Vitis vinifera])

HSP 1 Score: 553.132 bits (1424), Expect = 5.255e-155
Identity = 261/554 (47.11%), Postives = 369/554 (66.61%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            + HA++   G+  +  +  K   MY     +  A  +F  +    S  W+VM+ GF   G F +    + +++  G+ PD +  PF ++ C  + DL+ G+++H  ++  G   D F  AALVDMYAKC  ++ AR +FDKM  RDLV+WT MI  +A      E+L  FD M   GV+P++V++++++ AC  LGA+ +    H Y+ +  F  D+++ TA++DMY+KCG +D AR +FD    K+++ WSAMIA+YG HGHGRKAI +F  M+ +GV P+ +TF  +L ACSHSGL+EEG   F LM +EF +   + +Y CMVDLLGRAG+L EA++L+E+M VE D  +WG+LLGACRIH N+DLAEK  + L  L P +AG++VLLSNIYA   RW +V K+R +M++R   K+ G + +E DN+ ++F  GDR HPQ EK+YA LE L   ++  GYVP T+FVLHD++EE K A L  HSE+LAIAFGLI T+ GT +RITKNLR+CGDCH   K +S +  RVI+VRD++RFH F +G CSCGDYW
Sbjct:  738 KTHAKIFAYGLQYDSRILTKFAIMYVSFNRIDAASIVFEDIPNPCSFLWNVMIRGFATDGRFLSSLELYSKMMEKGLKPDKFAFPFALKSCAGLSDLQRGKVIHQHLVCCGCSNDLFVDAALVDMYAKCGDIEAARLVFDKMAVRDLVSWTSMISGYAHNGYNSETLGFFDLMRSSGVIPNRVSILSVLLACGNLGALRKGEWFHSYVIQTGFEFDILVATAIMDMYSKCGSLDLARCLFDETAGKDLVCWSAMIASYGIHGHGRKAIDLFDQMVKAGVRPSHVTFTCVLSACSHSGLLEEGKMYFQLMTEEFVIARKLSNYACMVDLLGRAGQLSEAVDLIENMPVEPDASIWGSLLGACRIHNNLDLAEKIADHLFHLDPVHAGYHVLLSNIYAAKSRWNEVEKVRKMMARRGANKIQGFSLVEYDNQVHKFGVGDRSHPQWEKLYAKLEELAAPMKHLGYVPLTDFVLHDIEEEAKEAALSYHSERLAIAFGLINTSPGTTLRITKNLRICGDCHNAIKLISKIVNRVILVRDMHRFHRFEDGVCSCGDYW 1291          

HSP 2 Score: 372.859 bits (956), Expect = 9.734e-101
Identity = 187/473 (39.53%), Postives = 292/473 (61.73%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLML-NSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHD 1419
            VH  V+      +L + N LL +YA+      A  LF  M E+D ++WS M+  + N          F E+I     P+  T+   ++ C   ++L+ G+ +H I +  G   D     AL+DMY KC   D+A  LF ++  +D+V+W  ++  +A+   A +S+ +F  M+ +G+ PD VA+V I+ A ++LG   +A  LH Y+ R+ F+ +V +G ++I++Y+KCG +  A ++F  M V++V+ WS+MIAAYG HG G +A+ IF  M+ NS V PN +TF+S+L ACSH+GL+EEGL+IF  M  ++ +R D +H+  MVDLLGR G+L +A++++  M +     +WGALLGACRIH NI++ E A  +L  L P +AG+Y+LLSNIYA  G+W +VA++R  + +R LKK+ G + +EV    + F   DR HP S+KIY +L  L  ++    Y+PD +F+LHD
Sbjct:  228 VHGLVIRREFDGDLPLVNSLLNLYAKTGCEKIAANLFSKMPEKDVISWSTMIACYANNEAANEALNLFHEMIEKRFEPNSVTVVSALQACAVSRNLEEGKKIHKIAVWKGFELDFSVSTALIDMYMKCSCPDEAVDLFQRLPKKDVVSWVALLSGYAQNGMAYKSMGVFRNMLSDGIQPDAVAVVKILAASSELGIFQQALCLHGYVVRSGFNSNVFVGASLIELYSKCGSLGDAVKLFKGMIVRDVVIWSSMIAAYGIHGRGGEALEIFDQMVKNSTVRPNNVTFLSILSACSHAGLVEEGLKIFDRMVHDYQLRPDSEHFGIMVDLLGRIGQLGKAMDIINRMPIPAGPHVWGALLGACRIHHNIEMGEAAAKNLFWLDPSHAGYYILLSNIYAVDGKWDNVAELRTRIKERGLKKMFGQSMVEVRGGVHSFLASDRFHPDSQKIYELLRKLEAQMGKEVYIPDLDFLLHD 700          

HSP 3 Score: 187.193 bits (474), Expect = 7.575e-45
Identity = 112/369 (30.35%), Postives = 200/369 (54.20%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELI-RSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLL-ADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMI-EEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSL-MWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            ++H+QV   G+L +   A KL  +YA+   L  A  +F      +   W+  +  +     +      F  +I  +G  PD +T+P  ++ C  ++ L++G+++H    K+  + +D F  +ALV++Y+KC  + +A ++F++ Q  D V WT M+  + +  + +E+L LF  M+  + VV D V +V++V+ACA+L  +     +H  + R +F  D+ L  +++++YAK GC   A  +F +M  K+VI+WS MIA Y  +    +A+ +FH M+     PN +T VS L AC+ S  +EEG +I  + +W  F +  D    T ++D+  +    DEA++L + +  +KD   W ALL     +G
Sbjct:   22 QLHSQVFKTGILHDTFFATKLNSLYAKCASLQAARKVFDETPHPNVHLWNSTLRSYCREKQWEETLRLFHLMICTAGEAPDNFTIPIALKACAGLRMLELGKVIHGFAKKNDEIGSDMFVGSALVELYSKCGQMGEALKVFEEFQRPDTVLWTSMVTGYQQNNDPEEALALFSQMVMMDCVVLDPVTLVSVVSACAQLLNVKAGSCVHGLVIRREFDGDLPLVNSLLNLYAKTGCEKIAANLFSKMPEKDVISWSTMIACYANNEAANEALNLFHEMIEKRFEPNSVTVVSALQACAVSRNLEEGKKIHKIAVWKGFEL--DFSVSTALIDMYMKCSCPDEAVDLFQRLP-KKDVVSWVALLSGYAQNG 387          

HSP 4 Score: 186.422 bits (472), Expect = 1.292e-44
Identity = 113/402 (28.11%), Postives = 209/402 (51.99%), Query Frame = 1

Query:   40 NLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSG-VTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLE---LQPENAGHYVLLSNIYARAGRWKDVAKMRDLM 1233
            ++ V + L+ +Y++   +G+A  +F      D+V W+ MV G+    D     A F +++    V  D  TL  V+  C  + ++K G  VH +V++     D     +L+++YAK      A  LF KM  +D+++W+ MI  +A    A+E+L LF  MIE+   P+ V +V+ + ACA    +   + +H       F LD  + TA+IDMY KC C D A ++F R+  K+V++W A+++ Y  +G   K++ +F  ML+ G+ P+ +  V +L A S  G+ ++ L +   +       +++     +++L  + G L +A++L + M V +D  +W +++ A  IHG    A +  + +++   ++P N     +LS   + AG  ++  K+ D M
Sbjct:  138 DMFVGSALVELYSKCGQMGEALKVFEEFQRPDTVLWTSMVTGYQQNNDPEEALALFSQMVMMDCVVLDPVTLVSVVSACAQLLNVKAGSCVHGLVIRREFDGDLPLVNSLLNLYAKTGCEKIAANLFSKMPEKDVISWSTMIACYANNEAANEALNLFHEMIEKRFEPNSVTVVSALQACAVSRNLEEGKKIHKIAVWKGFELDFSVSTALIDMYMKCSCPDEAVDLFQRLPKKDVVSWVALLSGYAQNGMAYKSMGVFRNMLSDGIQPDAVAVVKILAASSELGIFQQALCLHGYV-VRSGFNSNVFVGASLIELYSKCGSLGDAVKLFKGMIV-RDVVIWSSMIAAYGIHGRGGEALEIFDQMVKNSTVRPNNVTFLSILS-ACSHAGLVEEGLKIFDRM 536          

HSP 5 Score: 111.309 bits (277), Expect = 5.283e-22
Identity = 69/268 (25.75%), Postives = 130/268 (48.51%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVV-PDKVAMVTIVNACAKLGAMHRA-----RLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQV-KNVITWSAMIAAYGYH 783
            ++H   V  G   + +V+  L+ MY +     +A  LF  + ++D V+W  ++ G+   G        FR ++  G+ PD   +  ++    ++   +    +H  V++ G  ++ F  A+L+++Y+KC  + DA +LF  M  RD+V W+ MI A+       E+L +FD M++   V P+ V  ++I++AC+  G +        R++H Y    Q   D      M+D+  + G +  A +I +RM +      W A++ A   H
Sbjct:  328 KIHKIAVWKGFELDFSVSTALIDMYMKCSCPDEAVDLFQRLPKKDVVSWVALLSGYAQNGMAYKSMGVFRNMLSDGIQPDAVAVVKILAASSELGIFQQALCLHGYVVRSGFNSNVFVGASLIELYSKCGSLGDAVKLFKGMIVRDVVIWSSMIAAYGIHGRGGEALEIFDQMVKNSTVRPNNVTFLSILSACSHAGLVEEGLKIFDRMVHDY----QLRPDSEHFGIMVDLLGRIGQLGKAMDIINRMPIPAGPHVWGALLGACRIH 591          

BLAST of ppa025850m vs. NCBI nr
Match: gi|328774753|gb|AEB39776.1| (pentatricopeptide repeat protein 78 [Funaria hygrometrica])

HSP 1 Score: 541.962 bits (1395), Expect = 1.212e-151
Identity = 260/554 (46.93%), Postives = 364/554 (65.70%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            E+H++VV +G + +++V N L+ MYA+   + DA  LF  M  +D ++W+ M+GG    G      A F+++ ++G+ P+  T   ++  C     L  GR +H  V++ GL  D      LV+MY+ C  V DARQ+FD+M  RD+V +  MIG +A      E+L LFD + EEG+ PDKV  + ++NACA  G++  A+ +H  + ++ +  D  LG A++  YAKCG    A  +FD+M  +NVI+W+A+I     HG G+  + +F  M   G+ P+++TFVSLL ACSH+GL+EEG R F  M  +F +   I+HY CMVDLLGRAG+LDE   L+++M  + + R+WGALLGACRIHGN+ +AE+A  S L+L P+NA  YV LS++YA AG W   AK+R LM QR + K PG +WIEV +K + F   DR HP+SEKIYA L+ L   +++ GYVPDT  V+HDVDE  K   +  HSE+LAIA+GLI+T  GTPIRI KNLRVC DCHT TKF++ +  R I+ RDVNRFHHF++G CSCGDYW
Sbjct:  467 EIHSRVVKDGFMFDISVQNALISMYARCGSIKDARLLFNKMVRKDIISWTAMIGGLAKSGLGAEALAVFQDMQQAGLKPNRVTYTSILNACSSPAALDWGRRIHQQVIEAGLATDAHVANTLVNMYSMCGSVKDARQVFDRMTQRDIVAYNAMIGGYAAHNLGKEALKLFDRLQEEGLKPDKVTYINMLNACANSGSLEWAKEIHSLVLKDGYLSDTSLGNALVSTYAKCGSFSDALLVFDKMMKRNVISWNAIIGGCAQHGRGQDVLQLFERMKMEGIKPDIVTFVSLLSACSHAGLLEEGRRYFCSMSRDFGITPTIEHYGCMVDLLGRAGQLDEVEALIKTMPFQANTRIWGALLGACRIHGNVPVAERAAESSLKLDPDNAAVYVALSHMYAAAGMWDSAAKLRKLMEQRGVTKEPGRSWIEVGDKLHYFVAEDRSHPESEKIYAELDKLTHAMKMEGYVPDTRSVMHDVDEGEKENAVCHHSERLAIAYGLISTLPGTPIRIFKNLRVCPDCHTATKFITKIVDREIVARDVNRFHHFKDGVCSCGDYW 1020          

HSP 2 Score: 222.631 bits (566), Expect = 1.627e-55
Identity = 129/401 (32.17%), Postives = 218/401 (54.36%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLE--LQPENAGHYVLLSNIYARAG 1197
            E+H + +   +L ++ VAN +L MYA+   + +A  +F  M  +  V+W++++GG+ + G     F  F+++ + GV P+  T   V+        LK G+ VH  +L  G  +D     ALV MYAKC    D RQ+F+K+ NRDL+ W  MIG  AE  N +E+  ++  M  EG++P+K+  V ++NAC    A+H  R +H  + ++ F  D+ +  A+I MYA+CG I  AR +F++M  K++I+W+AMI      G G +A+A+F  M  +G+ PN +T+ S+L ACS    ++ G RI   +  E  +  D      +V++    G + +A ++ + M  ++D   + A++G    H     A K  + L E  L+P+    Y+ + N  A +G
Sbjct:  265 EIHVEAMKARLLFDVNVANCILNMYAKCGSIHEAREVFDKMETKSVVSWTIIIGGYADCGHSEIAFEIFQKMQQEGVVPNRITYINVLNAFSGPAALKWGKTVHSHILNAGHESDLAVGTALVKMYAKCGSYKDCRQVFEKLVNRDLIAWNTMIGGLAEGGNWEEASEIYHQMQREGMMPNKITYVILLNACVNPTALHWGREIHSRVVKDGFMFDISVQNALISMYARCGSIKDARLLFNKMVRKDIISWTAMIGGLAKSGLGAEALAVFQDMQQAGLKPNRVTYTSILNACSSPAALDWGRRIHQQV-IEAGLATDAHVANTLVNMYSMCGSVKDARQVFDRM-TQRDIVAYNAMIGGYAAHNLGKEALKLFDRLQEEGLKPDKV-TYINMLNACANSG 662          

HSP 3 Score: 214.927 bits (546), Expect = 3.393e-53
Identity = 108/364 (29.67%), Postives = 197/364 (54.12%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            VH+ ++  G   +L V   L+ MYA+     D   +F  +  RD + W+ M+GG    G++      + ++ R G+ P+  T   ++  C +   L  GR +H  V+K G + D     AL+ MYA+C  + DAR LF+KM  +D+++WT MIG  A+     E+L +F  M + G+ P++V   +I+NAC+   A+   R +H  +     + D  +   +++MY+ CG +  AR++FDRM  ++++ ++AMI  Y  H  G++A+ +F  +   G+ P+ +T++++L AC++SG +E    I SL+  +    +D      +V    + G   +AL + + M ++++   W A++G C  HG
Sbjct:  367 VHSHILNAGHESDLAVGTALVKMYAKCGSYKDCRQVFEKLVNRDLIAWNTMIGGLAEGGNWEEASEIYHQMQREGMMPNKITYVILLNACVNPTALHWGREIHSRVVKDGFMFDISVQNALISMYARCGSIKDARLLFNKMVRKDIISWTAMIGGLAKSGLGAEALAVFQDMQQAGLKPNRVTYTSILNACSSPAALDWGRRIHQQVIEAGLATDAHVANTLVNMYSMCGSVKDARQVFDRMTQRDIVAYNAMIGGYAAHNLGKEALKLFDRLQEEGLKPDKVTYINMLNACANSGSLEWAKEIHSLVLKD-GYLSDTSLGNALVSTYAKCGSFSDALLVFDKM-MKRNVISWNAIIGGCAQHG 728          

HSP 4 Score: 198.364 bits (503), Expect = 3.286e-48
Identity = 123/451 (27.27%), Postives = 227/451 (50.33%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMG--ERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYAC-----------SHSGLIEEG--------------------LRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESM---AVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYV-LLSNIYARAGRWKDVAKMRDLMSQR 1242
            EVH  ++ +  + +    N L+ MY Q   + +A  ++  +   ER   +W+ MV G+V  G         RE+ + G+     T   ++  C+    L+ GR +H   +K  LL D      +++MYAKC  + +AR++FDKM+ + +V+WT++IG +A+C +++ +  +F  M +EGVVP+++  + ++NA +   A+   + +H +I       D+ +GTA++ MYAKCG     R++F+++  +++I W+ MI      G+  +A  I+H M   G+ PN IT+V LL AC            HS ++++G                    ++   L++++  VR DI  +T M+  L ++G   EAL + + M    ++ +   + ++L AC     +D   +    ++E       H    L N+Y+  G  KD  ++ D M+QR
Sbjct:  162 EVHEHIIQHCTVLDQYTVNALINMYIQCGSIEEARQVWNKLNHTERTVHSWNAMVVGYVQYGYIEEALKLLREMQQHGLALGRATTMRLLSSCKSPSALECGREIHVEAMKARLLFDVNVANCILNMYAKCGSIHEAREVFDKMETKSVVSWTIIIGGYADCGHSEIAFEIFQKMQQEGVVPNRITYINVLNAFSGPAALKWGKTVHSHILNAGHESDLAVGTALVKMYAKCGSYKDCRQVFEKLVNRDLIAWNTMIGGLAEGGNWEEASEIYHQMQREGMMPNKITYVILLNACVNPTALHWGREIHSRVVKDGFMFDISVQNALISMYARCGSIKDARLLFNKM-VRKDIISWTAMIGGLAKSGLGAEALAVFQDMQQAGLKPNRVTYTSILNACSSPAALDWGRRIHQQVIEAGLATDAHVANTLVNMYSMCGSVKDARQVFDRMTQR 611          

HSP 5 Score: 152.525 bits (384), Expect = 2.068e-34
Identity = 83/310 (26.77%), Postives = 165/310 (53.23%), Query Frame = 1

Query:  259 VIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQN--RDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLE--LQPENAGHYVLLS 1176
            +++ C ++KDL  GR VH+ +++H  + D +   AL++MY +C  +++ARQ+++K+ +  R + +W  M+  + +    +E+L L   M + G+   +   + ++++C    A+   R +H    + +   DV +   +++MYAKCG I  ARE+FD+M+ K+V++W+ +I  Y   GH   A  IF  M   GV PN IT++++L A S    ++ G  + S + +     +D+   T +V +  + G   +  ++ E + V +D   W  ++G     GN + A +  + +    + P    + +LL+
Sbjct:  147 MLKRCIEVKDLVAGREVHEHIIQHCTVLDQYTVNALINMYIQCGSIEEARQVWNKLNHTERTVHSWNAMVVGYVQYGYIEEALKLLREMQQHGLALGRATTMRLLSSCKSPSALECGREIHVEAMKARLLFDVNVANCILNMYAKCGSIHEAREVFDKMETKSVVSWTIIIGGYADCGHSEIAFEIFQKMQQEGVVPNRITYINVLNAFSGPAALKWGKTVHSHILNA-GHESDLAVGTALVKMYAKCGSYKDCRQVFEKL-VNRDLIAWNTMIGGLAEGGNWEEASEIYHQMQREGMMPNKITYVILLN 454          

HSP 6 Score: 70.8626 bits (172), Expect = 7.912e-10
Identity = 45/209 (21.53%), Postives = 104/209 (49.76%), Query Frame = 1

Query:  490 DESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQ--VKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLA 1110
            D ++ +  ++ ++G   +    + ++  C ++  +   R +H +I ++   LD     A+I+MY +CG I+ AR++++++    + V +W+AM+  Y  +G+  +A+ +   M   G++    T + LL +C     +E G R   +   +  +  D+    C++++  + G + EA E+ + M   K    W  ++G     G+ ++A
Sbjct:  123 DRAMDVVQYLQQQGARVNSCDYMKMLKRCIEVKDLVAGREVHEHIIQHCTVLDQYTVNALINMYIQCGSIEEARQVWNKLNHTERTVHSWNAMVVGYVQYGYIEEALKLLREMQQHGLALGRATTMRLLSSCKSPSALECG-REIHVEAMKARLLFDVNVANCILNMYAKCGSIHEAREVFDKMET-KSVVSWTIIIGGYADCGHSEIA 329          

BLAST of ppa025850m vs. NCBI nr
Match: gi|168044807|ref|XP_001774871.1| (predicted protein [Physcomitrella patens subsp. patens] >gi|162673765|gb|EDQ60283.1| predicted protein [Physcomitrella patens subsp. patens])

HSP 1 Score: 528.094 bits (1359), Expect = 1.810e-147
Identity = 260/554 (46.93%), Postives = 359/554 (64.80%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            E+H++V   G   ++ V N L+ MY++   + DA  +F  M  +D ++W+ M+GG    G        ++E+ ++GV P+  T   ++  C     L+ GR +H  V++ GL  D      LV+MY+ C  V DARQ+FD+M  RD+V +  MIG +A      E+L LFD + EEG+ PDKV  + ++NACA  G++  AR +H  + +  F  D  +G A++  YAKCG    A  +F++M  +NVI+W+A+I     HG G+ A+ +F  M   GV P+++TFVSLL ACSH+GL+EEG R F  M  +FA+   I+HY CMVDLLGRAG+LDEA  L+++M  + + R+WGALLGACRIHGN+ +AE+A  S L+L  +NA  YV LS++YA AG W   AK+R LM QR + K PG +WI+V +K + F   DR HPQSEKIYA L+ L   +++ GYVPDT  V+HDVDE  K   +  HSE+LAIA+GLI+T  GT I I KNLRVC DCHT TKF+S +  R II RDVNRFHHF++G CSCGDYW
Sbjct:  349 EIHSRVAKAGFTSDIGVQNALISMYSRCGSIKDARLVFDKMVRKDVISWTAMIGGLAKSGFGAEALTVYQEMQQAGVEPNRVTYTSILNACSSPAALEWGRRIHQQVVEAGLATDAHVGNTLVNMYSMCGSVKDARQVFDRMIQRDIVAYNAMIGGYAAHNLGKEALKLFDRLQEEGLKPDKVTYINMLNACANSGSLEWAREIHTLVRKGGFFSDTSVGNALVSTYAKCGSFSDASIVFEKMTKRNVISWNAIIGGSAQHGRGQDALQLFERMKMEGVKPDIVTFVSLLSACSHAGLLEEGRRYFCSMSQDFAIIPTIEHYGCMVDLLGRAGQLDEAEALIKTMPFQANTRIWGALLGACRIHGNVPVAERAAESSLKLDLDNAVVYVALSHMYAAAGMWDSAAKLRKLMEQRGVTKEPGRSWIQVGDKLHYFVAEDRSHPQSEKIYAELDRLTHAMKMKGYVPDTRSVMHDVDEGEKENAVCHHSERLAIAYGLISTPPGTRIHIFKNLRVCPDCHTATKFISKIVDREIIARDVNRFHHFKDGVCSCGDYW 902          

HSP 2 Score: 228.409 bits (581), Expect = 2.965e-57
Identity = 131/401 (32.67%), Postives = 219/401 (54.61%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLE--LQPENAGHYVLLSNIYARAG 1197
            E+H Q +  G+L ++ VAN +L MYA+   + +A  +F  M ++  V+W++ +GG+ + G     F  F+++ + GV P+  T   V+        LK G+ VH  +L  G  +D     ALV MYAKC    D RQ+F+K+ NRDL+ W  MIG  AE    +E+  +++ M  EGV+P+K+  V ++NAC    A+H  + +H  + +  F+ D+ +  A+I MY++CG I  AR +FD+M  K+VI+W+AMI      G G +A+ ++  M  +GV PN +T+ S+L ACS    +E G RI   +  E  +  D      +V++    G + +A ++ + M +++D   + A++G    H     A K  + L E  L+P+    Y+ + N  A +G
Sbjct:  147 EIHFQAMQAGLLFDVKVANCILNMYAKCGSIEEAREVFDKMEKKSVVSWTITIGGYADCGRSETAFEIFQKMEQEGVVPNRITYISVLNAFSSPAALKWGKAVHSRILNAGHESDTAVGTALVKMYAKCGSYKDCRQVFEKLVNRDLIAWNTMIGGLAEGGYWEEASEVYNQMQREGVMPNKITYVILLNACVNSAALHWGKEIHSRVAKAGFTSDIGVQNALISMYSRCGSIKDARLVFDKMVRKDVISWTAMIGGLAKSGFGAEALTVYQEMQQAGVEPNRVTYTSILNACSSPAALEWGRRIHQQV-VEAGLATDAHVGNTLVNMYSMCGSVKDARQVFDRM-IQRDIVAYNAMIGGYAAHNLGKEALKLFDRLQEEGLKPDKV-TYINMLNACANSG 544          

HSP 3 Score: 204.527 bits (519), Expect = 4.585e-50
Identity = 105/364 (28.85%), Postives = 193/364 (53.02%), Query Frame = 1

Query:    4 VHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHG 1095
            VH++++  G   +  V   L+ MYA+     D   +F  +  RD + W+ M+GG    G +      + ++ R GV P+  T   ++  C +   L  G+ +H  V K G  +D     AL+ MY++C  + DAR +FDKM  +D+++WT MIG  A+     E+L ++  M + GV P++V   +I+NAC+   A+   R +H  +     + D  +G  +++MY+ CG +  AR++FDRM  ++++ ++AMI  Y  H  G++A+ +F  +   G+ P+ +T++++L AC++SG +E    I +L+  +    +D      +V    + G   +A  + E M  +++   W A++G    HG
Sbjct:  249 VHSRILNAGHESDTAVGTALVKMYAKCGSYKDCRQVFEKLVNRDLIAWNTMIGGLAEGGYWEEASEVYNQMQREGVMPNKITYVILLNACVNSAALHWGKEIHSRVAKAGFTSDIGVQNALISMYSRCGSIKDARLVFDKMVRKDVISWTAMIGGLAKSGFGAEALTVYQEMQQAGVEPNRVTYTSILNACSSPAALEWGRRIHQQVVEAGLATDAHVGNTLVNMYSMCGSVKDARQVFDRMIQRDIVAYNAMIGGYAAHNLGKEALKLFDRLQEEGLKPDKVTYINMLNACANSGSLEWAREIHTLV-RKGGFFSDTSVGNALVSTYAKCGSFSDASIVFEKM-TKRNVISWNAIIGGSAQHG 610          

HSP 4 Score: 202.986 bits (515), Expect = 1.334e-49
Identity = 123/451 (27.27%), Postives = 224/451 (49.67%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMG--ERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFS-------------------------------LMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESM---AVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHY-VLLSNIYARAGRWKDVAKMRDLMSQR 1242
            +VH  ++ +  + +    N L+ MY Q   + +A  ++  +   ER   +W+ MV G++  G         R++ + G+ PD  T+   +  C+    L+ GR +H   ++ GLL D      +++MYAKC  +++AR++FDKM+ + +V+WT+ IG +A+C  ++ +  +F  M +EGVVP+++  ++++NA +   A+   + +H  I       D  +GTA++ MYAKCG     R++F+++  +++I W+ MI      G+  +A  +++ M   GV PN IT+V LL AC +S  +  G  I S                               L++D+  VR D+  +T M+  L ++G   EAL + + M    VE +   + ++L AC     ++   +    ++E       H    L N+Y+  G  KD  ++ D M QR
Sbjct:   44 QVHQHIIQHRTVPDQYTVNALINMYIQCGSIEEARQVWKKLSYMERTVHSWNAMVVGYIQYGYIEKALKLLRQMQQHGLAPDRTTIMSFLSSCKSPGALEWGREIHFQAMQAGLLFDVKVANCILNMYAKCGSIEEAREVFDKMEKKSVVSWTITIGGYADCGRSETAFEIFQKMEQEGVVPNRITYISVLNAFSSPAALKWGKAVHSRILNAGHESDTAVGTALVKMYAKCGSYKDCRQVFEKLVNRDLIAWNTMIGGLAEGGYWEEASEVYNQMQREGVMPNKITYVILLNACVNSAALHWGKEIHSRVAKAGFTSDIGVQNALISMYSRCGSIKDARLVFDKM-VRKDVISWTAMIGGLAKSGFGAEALTVYQEMQQAGVEPNRVTYTSILNACSSPAALEWGRRIHQQVVEAGLATDAHVGNTLVNMYSMCGSVKDARQVFDRMIQR 493          

HSP 5 Score: 156.377 bits (394), Expect = 1.432e-35
Identity = 85/310 (27.42%), Postives = 163/310 (52.58%), Query Frame = 1

Query:  259 VIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKM--QNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLE--LQPENAGHYVLLS 1176
            +++ C ++KDL  GR VH  +++H  + D +   AL++MY +C  +++ARQ++ K+    R + +W  M+  + +    +++L L   M + G+ PD+  +++ +++C   GA+   R +H+   +     DV +   +++MYAKCG I+ ARE+FD+M+ K+V++W+  I  Y   G    A  IF  M   GV PN IT++S+L A S    ++ G  + S + +     +D    T +V +  + G   +  ++ E + V +D   W  ++G     G  + A +  N +    + P    + +LL+
Sbjct:   29 MLKRCIEVKDLVAGRQVHQHIIQHRTVPDQYTVNALINMYIQCGSIEEARQVWKKLSYMERTVHSWNAMVVGYIQYGYIEKALKLLRQMQQHGLAPDRTTIMSFLSSCKSPGALEWGREIHFQAMQAGLLFDVKVANCILNMYAKCGSIEEAREVFDKMEKKSVVSWTITIGGYADCGRSETAFEIFQKMEQEGVVPNRITYISVLNAFSSPAALKWGKAVHSRILNA-GHESDTAVGTALVKMYAKCGSYKDCRQVFEKL-VNRDLIAWNTMIGGLAEGGYWEEASEVYNQMQREGVMPNKITYVILLN 336          

HSP 6 Score: 82.0333 bits (201), Expect = 3.432e-13
Identity = 48/199 (24.12%), Postives = 104/199 (52.26%), Query Frame = 1

Query:  490 DESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQV--KNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRI-FSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLG 1077
            D ++ +  ++ ++G   +    + ++  C ++  +   R +H +I +++   D     A+I+MY +CG I+ AR+++ ++    + V +W+AM+  Y  +G+  KA+ +   M   G++P+  T +S L +C   G +E G  I F  M  +  +  D+K   C++++  + G ++EA E+ + M  +K    W   +G
Sbjct:    5 DGAVDVVQYLQQQGAQVNSSDYMKMLKRCIEVKDLVAGRQVHQHIIQHRTVPDQYTVNALINMYIQCGSIEEARQVWKKLSYMERTVHSWNAMVVGYIQYGYIEKALKLLRQMQQHGLAPDRTTIMSFLSSCKSPGALEWGREIHFQAM--QAGLLFDVKVANCILNMYAKCGSIEEAREVFDKME-KKSVVSWTITIG 200          

BLAST of ppa025850m vs. NCBI nr
Match: gi|148909481|gb|ABR17838.1| (unknown [Picea sitchensis])

HSP 1 Score: 520.39 bits (1339), Expect = 3.775e-145
Identity = 247/554 (44.58%), Postives = 369/554 (66.61%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHYTCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPENAGHYVLLSNIYARAGRWKDVAKMRDLMSQRRLKKVPGLTWIEVDNKTYQFSTGDRIHPQSEKIYAMLESLGKKLELAGYVPDTNFVLHDVDEEVKLAMLYSHSEKLAIAFGLIATADGTPIRITKNLRVCGDCHTFTKFVSAVTQRVIIVRDVNRFHHFREGTCSCGDYW 1662
            ++H   + +G+  ++ V N L+ MYA+   +  A+ LF  M  RD  +W+ ++GG+          A F  +   G+ P+  T+  V+  C  +  L+ G+ +H   ++ G  +++    ALV+MYAKC  V+ A +LF++M  +++V W  +I  +++  +  E+L LF  M  +G+ PD  A+V+++ ACA   A+ + + +H Y  R+ F  +V++GT ++D+YAKCG +++A+++F+RM  ++V++W+ MI AYG HGHG  A+A+F  M  +G   + I F ++L ACSH+GL+++GL+ F  M  ++ +   ++HY C+VDLLGRAG LDEA  ++++M++E D  +WGALLGACRIH NI+L E+A   L EL P+NAG+YVLLSNIYA A RW+DVAK+R +M ++ +KK PG + + V      F  GDR HPQSE+IYAMLE L +++  AGYVP+TN  L DV+EE K  +L SHSEKLAI+FG+I T+ G PIRI KNLRVC DCH  TKF+S +  R IIVRD NRFHH + G CSCGDYW
Sbjct:  242 QIHCYAIRSGIESDVLVVNGLVNMYAKCGNVNTAHKLFERMPIRDVASWNAIIGGYSLNSQHHEALAFFNRMQVRGIKPNSITMVSVLPACAHLFALEQGQQIHGYAIRSGFESNDVVGNALVNMYAKCGNVNSAYKLFERMPKKNVVAWNAIISGYSQHGHPHEALALFIEMQAQGIKPDSFAIVSVLPACAHFLALEQGKQIHGYTIRSGFESNVVVGTGLVDIYAKCGNVNTAQKLFERMPEQDVVSWTTMILAYGIHGHGEDALALFSKMQETGTKLDHIAFTAILTACSHAGLVDQGLQYFQCMKSDYGLAPKLEHYACLVDLLGRAGHLDEANGIIKNMSLEPDANVWGALLGACRIHCNIELGEQAAKHLFELDPDNAGYYVLLSNIYAEAQRWEDVAKLRKMMKEKGVKKQPGCSVVAVHRDVQTFLVGDRTHPQSEQIYAMLEILYEQMRKAGYVPNTNLALQDVEEEAKENILSSHSEKLAISFGIINTSPGIPIRIMKNLRVCSDCHNATKFISKIVGREIIVRDANRFHHVKNGFCSCGDYW 795          

HSP 2 Score: 224.557 bits (571), Expect = 4.282e-56
Identity = 120/370 (32.43%), Postives = 209/370 (56.49%), Query Frame = 1

Query:    1 EVHAQVVVNGMLQNLTVANKLLYMYAQRRVLGDAYALFGGMGERDSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHY----TCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGN 1098
            +VH  ++  G   ++ V   L  MY +   L +A  +F  M +RD V+W+ ++ G+   G      A F E+  +G+ P+  TL  V+ VC  +  L+ G+ +H   ++ G+ +D      LV+MYAKC  V+ A +LF++M  RD+ +W  +IG ++      E+L  F+ M   G+ P+ + MV+++ ACA L A+ + + +H Y  R+ F  + ++G A+++MYAKCG ++SA ++F+RM  KNV+ W+A+I+ Y  HGH  +A+A+F  M   G+ P+    VS+L AC+H   +E+G +I       + +R+  +      T +VD+  + G ++ A +L E M  E+D   W  ++ A  IHG+
Sbjct:  141 KVHEDIIARGFESDVIVGTALASMYTKCGSLENARQVFDRMPKRDVVSWNAIIAGYSQNGQPYEALALFSEMQVNGIKPNSSTLVSVMPVCAHLLALEQGKQIHCYAIRSGIESDVLVVNGLVNMYAKCGNVNTAHKLFERMPIRDVASWNAIIGGYSLNSQHHEALAFFNRMQVRGIKPNSITMVSVLPACAHLFALEQGQQIHGYAIRSGFESNDVVGNALVNMYAKCGNVNSAYKLFERMPKKNVVAWNAIISGYSQHGHPHEALALFIEMQAQGIKPDSFAIVSVLPACAHFLALEQGKQIHG-----YTIRSGFESNVVVGTGLVDIYAKCGNVNTAQKLFERMP-EQDVVSWTTMILAYGIHGH 504          

HSP 3 Score: 199.519 bits (506), Expect = 1.475e-48
Identity = 103/343 (30.03%), Postives = 190/343 (55.39%), Query Frame = 1

Query:  133 DSVTWSVMVGGFVNVGDFTNGFATFRELIRSGVTPDIYTLPFVIRVCRDMKDLKMGRLVHDIVLKHGLLADNFACAALVDMYAKCKVVDDARQLFDKMQNRDLVTWTVMIGAFAECRNADESLVLFDWMIEEGVVPDKVAMVTIVNACAKLGAMHRARLLHYYICRNQFSLDVILGTAMIDMYAKCGCIDSAREIFDRMQVKNVITWSAMIAAYGYHGHGRKAIAIFHLMLNSGVSPNVITFVSLLYACSHSGLIEEGLRIFSLMWDEFAVRADIKHY----TCMVDLLGRAGRLDEALELVESMAVEKDERLWGALLGACRIHGNIDLAEKAVNSLLELQPE 1149
            ++V W   + G+V  G +      + ++ R+G+ PD      VI+ C    DL+ GR VH+ ++  G  +D     AL  MY KC  +++ARQ+FD+M  RD+V+W  +I  +++     E+L LF  M   G+ P+   +V+++  CA L A+ + + +H Y  R+    DV++   +++MYAKCG +++A ++F+RM +++V +W+A+I  Y  +    +A+A F+ M   G+ PN IT VS+L AC+H   +E+G +I       +A+R+  +        +V++  + G ++ A +L E M  +K+   W A++     HG+     +A+   +E+Q +
Sbjct:   84 NAVVWKETIIGYVKNGFWNKALRLYYQMQRTGINPDKLVFLSVIKACGSQSDLQAGRKVHEDIIARGFESDVIVGTALASMYTKCGSLENARQVFDRMPKRDVVSWNAIIAGYSQNGQPYEALALFSEMQVNGIKPNSSTLVSVMPVCAHLLALEQGKQIHCYAIRSGIESDVLVVNGLVNMYAKCGNVNTAHKLFERMPIRDVASWNAIIGGYSLNSQHHEALAFFNRMQVRGIKPNSITMVSVLPACAHLFALEQGQQIHG-----YAIRSGFESNDVVGNALVNMYAKCGNVNSAYKLFERMP-KKNVVAWNAIISGYSQHGH---PHEALALFIEMQAQ 417