Fragaria viridis YNU Genome v1.0 Assembly & Annotation

Analysis NameFragaria viridis YNU Genome v1.0 Assembly & Annotation
MethodNanopore PromethION, NextDenovo (v2.4)
SourceFragaria viridis YNG Genome v1.0
Date performed2021-09-27


Qiao Q, Edger PP, Xue L, La Q, Lu J, Zhang YC, Alan EY, Adrian EP, Steven JK, Montagu MV, Van de Peer Y, Lei JJ, Zhang TC. Evolutionary history and pan-genome dynamics of strawberry (Fragaria spp.). 2021. Accepted to PNAS



Strawberry (Fragaria spp.) has emerged as a model system for various fundamental and applied research in recent years. In total, the genomes of five different species have been sequenced over the past ten years. Here, we report chromosome-scale reference genomes for five strawberry species, including three newly sequenced species’ genomes, and genome re-sequencing data for 128 additional accessions to estimate the genetic diversity and structure of key Fragaria species. Our analyses obtained fully resolved and strongly supported phylogenies and divergence times for most diploid strawberry species. These analyses also uncovered a new diploid species (F. emeiensis Lei). Lastly, we constructed a pan-genome for Fragaria and examined the evolutionary dynamics of gene families associated with important agronomic traits. Notably, we identified multiple independent single base mutations of the MYB10 gene associated with white pigmented fruit shared by different strawberry species. These new reference genomes and datasets, combined with our new phylogenetic estimates, should serve as a powerful comparative genomic platform and resource for future studies in strawberry.


The Fragaria viridis Genome v1.0 assembly file is available in FASTA format.


Chromosomes (FASTA file) fviridis_v1.0.fasta.gz
Repeats (GFF3 file) fviridis_v1.0.repeats.gff3.gz


Gene Predictions

The Fragaria viridis v1.0 genome gene prediction files are available in FASTA and GFF3 formats.


Protein sequences  (FASTA file) fviridis_v1.0.proteins.fasta.gz
CDS  (FASTA file) fviridis_v1.0.cds.fasta.gz
Genes (GFF3 file) fviridis_v1.0.genes.gff3.gz


Transcript Alignments
Transcript alignments were performed by the GDR Team of Main Bioinformatics Lab at WSU. The alignment tool 'BLAT' was used to map transcripts to the Fragaria viridis genome assembly. Alignments with an alignment length of 97% and 97% identify were preserved. The available files are in GFF3 format.


Fragaria x ananassa GDR RefTrans v1 fviridis_v1.0_f.x.ananassa_GDR_reftransV1
fragaria avium GDR RefTrans v1 fviridis_v1.0_p.avium_GDR_reftransV1
fragaria persica GDR RefTrans v1 fviridis_v1.0_p.persica_GDR_reftransV1
Rosa GDR RefTrans v1 fviridis_v1.0_rosa_GDR_reftransV1
Rubus GDR RefTrans v2 fviridis_v1.0_rubus_GDR_reftransV2
Malus_x_domestica GDR RefTrans v1 fviridis_v1.0_m.x.domestica_GDR_reftransV1
Pyrus GDR RefTrans v1 fviridis_v1.0_pyrus_GDR_reftransV1