Microsatellite markers for Fragaria from 'Strawberry Festival' expressed sequence tags

We present 37 microsatellite primer pairs developed from a cDNA library of Fragaria xananassa Duch. cv. Strawberry Festival. Polymorphism was high and the number of presumptive alleles of 13 expressed sequence tag-simple sequence repeats (EST-SSRs) in 70 strawberry cultivars ranged from five to 32 per primer pairs, averaging 16.1. Cross-species amplification was also high and ranged from 89% in Fragaria vesca L. to 100% in the progenitor species of octoploid strawberry, Fragaria chiloensis (L.) Duch. and Fragaria virginiana Duch.