Deciphering gamma-decalactone biosynthesis in strawberry fruit using a combination of genetic mapping, RNA-Seq and eQTL analyses

Publication Overview
TitleDeciphering gamma-decalactone biosynthesis in strawberry fruit using a combination of genetic mapping, RNA-Seq and eQTL analyses
AuthorsSanchez-Sevilla JF, Crus-Rus E, Valpuesta V, Botella MA, Amaya I.
TypeJournal Article
Journal NameBMC Genomics
Volume15
Year2014
Page(s)218
CitationSanchez-Sevilla JF, Crus-Rus E, Valpuesta V, Botella MA, Amaya I.. Deciphering gamma-decalactone biosynthesis in strawberry fruit using a combination of genetic mapping, RNA-Seq and eQTL analyses. BMC Genomics. 2014; 15:218.

Abstract

Background Understanding the basis for volatile organic compound (VOC) biosynthesis and regulation is of great importance for the genetic improvement of fruit flavor. Lactones constitute an essential group of fatty acid-derived VOCs conferring peach-like aroma to a number of fruits including peach, plum, pineapple and strawberry. Early studies on lactone biosynthesis suggest that several enzymatic pathways could be responsible for the diversity of lactones, but detailed information on them remained elusive. In this study, we have integrated genetic mapping and genome-wide transcriptome analysis to investigate the molecular basis of natural variation in γ-decalactone content in strawberry fruit. Results As a result, the fatty acid desaturase FaFAD1 was identified as the gene underlying the locus at LGIII-2 that controls γ-decalactone production in ripening fruit. The FaFAD1 gene is specifically expressed in ripe fruits and its expression fully correlates with the presence of γ-decalactone in all 95 individuals of the mapping population. In addition, we show that the level of expression of FaFAH1, with similarity to cytochrome p450 hydroxylases, significantly correlates with the content of γ-decalactone in the mapping population. The analysis of expression quantitative trait loci (eQTL) suggests that the product of this gene also has a regulatory role in the biosynthetic pathway of lactones. Conclusions Altogether, this study provides mechanistic information of how the production of γ-decalactone is naturally controlled in strawberry, and proposes enzymatic activities necessary for the formation of this VOC in plants.
Projects
This publication contains information about 1 projects:
Project NameDescription
Strawberry-Decalactone_biosynthesis-Sanchez-Sevilla-2014
Features
This publication contains information about 397 features:
Feature NameUniquenameType
F23688-7:C>AF23688-7:C>Agenetic_marker
F23715-36:G>TF23715-36:G>Tgenetic_marker
F23855-57:C>TF23855-57:C>Tgenetic_marker
F24164-18:C>AF24164-18:C>Agenetic_marker
F24350-48:A>GF24350-48:A>Ggenetic_marker
F24406-46:G>AF24406-46:G>Agenetic_marker
F24567-23:T>AF24567-23:T>Agenetic_marker
F25553-34:C>TF25553-34:C>Tgenetic_marker
F25851-64:T>CF25851-64:T>Cgenetic_marker
F25871-35:C>TF25871-35:C>Tgenetic_marker
F25995-36:T>GF25995-36:T>Ggenetic_marker
F26316-58:G>TF26316-58:G>Tgenetic_marker
F26366-53:T>CF26366-53:T>Cgenetic_marker
F27004-13:C>AF27004-13:C>Agenetic_marker
F27089-47:C>TF27089-47:C>Tgenetic_marker
F27572-45:T>AF27572-45:T>Agenetic_marker
F27869-15:C>AF27869-15:C>Agenetic_marker
F28001-24:C>GF28001-24:C>Ggenetic_marker
F28198-22:G>CF28198-22:G>Cgenetic_marker
F28286-9:T>CF28286-9:T>Cgenetic_marker
F28451-58:G>AF28451-58:G>Agenetic_marker
F28471-20:G>CF28471-20:G>Cgenetic_marker
F28568-25:C>GF28568-25:C>Ggenetic_marker
F28600-55:G>AF28600-55:G>Agenetic_marker
F28691-49:G>TF28691-49:G>Tgenetic_marker

Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
Strawberry-232x1392-F1-2014